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Q5U4C1

- GASP1_MOUSE

UniProt

Q5U4C1 - GASP1_MOUSE

Protein

G-protein coupled receptor-associated sorting protein 1

Gene

Gprasp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN1P1/BECN2.1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. endosome to lysosome transport Source: UniProtKB
    2. G-protein coupled receptor catabolic process Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-protein coupled receptor-associated sorting protein 1
    Short name:
    GASP-1
    Gene namesi
    Name:Gprasp1
    Synonyms:Kiaa0443
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1917418. Gprasp1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13471347G-protein coupled receptor-associated sorting protein 1PRO_0000239051Add
    BLAST

    Proteomic databases

    MaxQBiQ5U4C1.
    PaxDbiQ5U4C1.
    PRIDEiQ5U4C1.

    PTM databases

    PhosphoSiteiQ5U4C1.

    Expressioni

    Tissue specificityi

    Expressed in the brain, with higher expression in the hippocampus, hypothalamus and olfactory bulb.1 Publication

    Gene expression databases

    BgeeiQ5U4C1.
    GenevestigatoriQ5U4C1.

    Interactioni

    Subunit structurei

    Interacts with cytoplasmic tails of a variety of G-protein coupled receptors such as delta opioid receptor/OPRD1, beta-2 adrenergic receptor/ADRB2 and D4 dopamine receptor/DRD4 as well as D2 dopamine receptor/DRD2. Interacts with PER1 By similarity. Interacts with BECN1P1/BECN2; the interaction is direct.By similarity1 Publication

    Protein-protein interaction databases

    IntActiQ5U4C1. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U4C1.
    SMRiQ5U4C1. Positions 1108-1160.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1109 – 11124Poly-Leu

    Sequence similaritiesi

    Belongs to the GPRASP family.Curated

    Phylogenomic databases

    eggNOGiNOG136667.
    GeneTreeiENSGT00550000074488.
    HOVERGENiHBG057167.
    InParanoidiA2AGB6.
    OMAiQADDESI.
    OrthoDBiEOG70PBZ0.
    PhylomeDBiQ5U4C1.
    TreeFamiTF335652.

    Family and domain databases

    InterProiIPR006911. ARM-rpt_dom.
    IPR016024. ARM-type_fold.
    [Graphical view]
    PfamiPF04826. Arm_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5U4C1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTRAEVEPGA QAKAENKPGD ENANAAEVEP EAPLVVRPKV RTQIMTGARP     50
    KVKPKGTPGA RPKGETSTPG GAYAKCKPKA IPIARSKHDA QVWAPNKFRG 100
    ESMSKMGKQC QISAADPPLL SNDSGMVAQA KCLPVDRELA NMDTESIPKK 150
    ANSPAGFQPS YGSEEGTNMG SWYRARPVPK GEAYENSDFK WADKPSGSPS 200
    FWNRDEASTR FRPRKSMKAN NRFRHMAKQE ANTMPRHKNK QEFYNISSSD 250
    SEDESGKTPW FWPKDKTKVW SKPKEEPNSR SWFRSKKEVR VESTSGSECE 300
    NPTKSLFWSG EEAKSRSKPR ARKGVNMRAR QQAKREACSD AMSGAIDTNK 350
    KESWFLPEEK ANVFSKSKTK KEPRTRAVPK EEVKTKARAS TKQEARPEEE 400
    VLVGAWFWDT QESTMADRIS IKTTFVEEEP IVGDWFWSEE EASVDSETCH 450
    TSRPRAKEEQ VSSFCLGSGK KSSMESGPKA TSKSMPVAKE DEVVIGSWFW 500
    ADDEEINLQA DDESIFGSWF WGTGENSLRS VGVNCEKMPK AGEKEVTDSW 550
    FWAGDVNTEA EVEEQARSAS TKATIFVPWF WSEKQPNMDL GSEPCSDIMA 600
    GAEEEPIIGP WFWAKVDNSV EAEVNSKSSL EDEEEPIRSP WFGAREQTDM 650
    KYAAGIRYKP MAEAEDANKK SCVWAKEPCL YPTNRECLKS TLGEKEDTVD 700
    PWLWSNNYPR TKTITGSWLW AAEEGNIDDE TGEKIKLPTL EDNAFNSWFW 750
    KENEESIVEA PKREEFRPEA EEEDIIGSWF WAGDEDRFEP AAKINEENKI 800
    ASEDEDTVGS WFWGNEEASL EAVRRGTFES APGIKEEKVT GSWFWTDKAK 850
    VGAGSQTVET GSETEEEAIF ESLIWAAKKD SIQAGVKRVS KPKDDGNIAV 900
    GSWLWSSDKA TKEAKTLIVS EASPENGKES VVKFGSRAKD EVINKTGSGD 950
    NCKHSTEAET IVGAWFWEGD EASFESNPVP VCKAVCEPES SAEHEPDPSR 1000
    RPQSWDEVTV QFKAGPWGKA GFPPMNPFRF PKEAASLFAE MFGGKPKLVE 1050
    VGPEREPEPQ FPFQYDPSYR SVREIREHLK ARESAQPENW SCNCIQCELR 1100
    IGSEEFEELL LLMDRNRDPF IHEISKIAMG MRGASQFTRD FIRNSGVVSL 1150
    IEALLNYPSS RVRTRFLENM VRMAPPYPDL NMIETYVCQI CEDTFDYDLD 1200
    SPDQLSGLTM ITHLTATSDY HKVVVNYLAG FFYLLNSGNT KTRFHVLKLL 1250
    LNLSENLVMT KRLLVTDSVS EFMDLINREE SDENIQIVLA IFETISKHIQ 1300
    KEALFSDDDD DDEEEDAVNL EPFISAFREA EKIAKELKRK PGNQKAP 1347
    Length:1,347
    Mass (Da):151,745
    Last modified:December 7, 2004 - v1
    Checksum:i1FD29E59CBF91B59
    GO
    Isoform 2 (identifier: Q5U4C1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-473: Missing.

    Show »
    Length:874
    Mass (Da):98,825
    Checksum:i403D64EEC544823D
    GO

    Sequence cautioni

    The sequence BAC41425.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti942 – 9421V → L in BAC41425. (PubMed:12465718)Curated
    Sequence conflicti943 – 9431I → F in BAC30259. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 473473Missing in isoform 2. 1 PublicationVSP_019080Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB093241 mRNA. Translation: BAC41425.1. Different initiation.
    AL683822 Genomic DNA. Translation: CAM27375.1.
    BC027187 mRNA. Translation: AAH27187.1.
    BC085157 mRNA. Translation: AAH85157.1.
    AK039156 mRNA. Translation: BAC30259.1.
    AK050919 mRNA. Translation: BAC34459.2.
    AK083192 mRNA. Translation: BAC38803.1.
    CCDSiCCDS30409.1. [Q5U4C1-1]
    RefSeqiNP_001004359.1. NM_001004359.2. [Q5U4C1-1]
    NP_001005385.1. NM_001005385.1. [Q5U4C1-1]
    NP_080357.4. NM_026081.5. [Q5U4C1-1]
    UniGeneiMm.271980.

    Genome annotation databases

    EnsembliENSMUST00000113144; ENSMUSP00000108769; ENSMUSG00000043384. [Q5U4C1-1]
    ENSMUST00000113145; ENSMUSP00000108770; ENSMUSG00000043384. [Q5U4C1-1]
    ENSMUST00000113147; ENSMUSP00000108772; ENSMUSG00000043384. [Q5U4C1-1]
    ENSMUST00000166554; ENSMUSP00000132225; ENSMUSG00000043384. [Q5U4C1-1]
    GeneIDi67298.
    KEGGimmu:67298.
    UCSCiuc009uhn.1. mouse. [Q5U4C1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB093241 mRNA. Translation: BAC41425.1 . Different initiation.
    AL683822 Genomic DNA. Translation: CAM27375.1 .
    BC027187 mRNA. Translation: AAH27187.1 .
    BC085157 mRNA. Translation: AAH85157.1 .
    AK039156 mRNA. Translation: BAC30259.1 .
    AK050919 mRNA. Translation: BAC34459.2 .
    AK083192 mRNA. Translation: BAC38803.1 .
    CCDSi CCDS30409.1. [Q5U4C1-1 ]
    RefSeqi NP_001004359.1. NM_001004359.2. [Q5U4C1-1 ]
    NP_001005385.1. NM_001005385.1. [Q5U4C1-1 ]
    NP_080357.4. NM_026081.5. [Q5U4C1-1 ]
    UniGenei Mm.271980.

    3D structure databases

    ProteinModelPortali Q5U4C1.
    SMRi Q5U4C1. Positions 1108-1160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q5U4C1. 1 interaction.

    PTM databases

    PhosphoSitei Q5U4C1.

    Proteomic databases

    MaxQBi Q5U4C1.
    PaxDbi Q5U4C1.
    PRIDEi Q5U4C1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000113144 ; ENSMUSP00000108769 ; ENSMUSG00000043384 . [Q5U4C1-1 ]
    ENSMUST00000113145 ; ENSMUSP00000108770 ; ENSMUSG00000043384 . [Q5U4C1-1 ]
    ENSMUST00000113147 ; ENSMUSP00000108772 ; ENSMUSG00000043384 . [Q5U4C1-1 ]
    ENSMUST00000166554 ; ENSMUSP00000132225 ; ENSMUSG00000043384 . [Q5U4C1-1 ]
    GeneIDi 67298.
    KEGGi mmu:67298.
    UCSCi uc009uhn.1. mouse. [Q5U4C1-1 ]

    Organism-specific databases

    CTDi 9737.
    MGIi MGI:1917418. Gprasp1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG136667.
    GeneTreei ENSGT00550000074488.
    HOVERGENi HBG057167.
    InParanoidi A2AGB6.
    OMAi QADDESI.
    OrthoDBi EOG70PBZ0.
    PhylomeDBi Q5U4C1.
    TreeFami TF335652.

    Miscellaneous databases

    ChiTaRSi GPRASP1. mouse.
    NextBioi 324156.
    PROi Q5U4C1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q5U4C1.
    Genevestigatori Q5U4C1.

    Family and domain databases

    InterProi IPR006911. ARM-rpt_dom.
    IPR016024. ARM-type_fold.
    [Graphical view ]
    Pfami PF04826. Arm_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
      DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain and Eye.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-379 AND 943-1347 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo, Hippocampus and Hypothalamus.
    5. "Identification of a novel family of G protein-coupled receptor associated sorting proteins."
      Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
      J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism."
      He C., Wei Y., Sun K., Li B., Dong X., Zou Z., Liu Y., Kinch L.N., Khan S., Sinha S., Xavier R.J., Grishin N.V., Xiao G., Eskelinen E.L., Scherer P.E., Whistler J.L., Levine B.
      Cell 154:1085-1099(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BECN1P1/BECN2.

    Entry informationi

    Entry nameiGASP1_MOUSE
    AccessioniPrimary (citable) accession number: Q5U4C1
    Secondary accession number(s): A2AGB6
    , Q8BKR8, Q8BUN4, Q8BYK9, Q8CHF4, Q8R095
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3