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Q5U4C1 (GASP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor-associated sorting protein 1

Short name=GASP-1
Gene names
Name:Gprasp1
Synonyms:Kiaa0443
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN1P1/BECN2. Ref.6

Subunit structure

Interacts with cytoplasmic tails of a variety of G-protein coupled receptors such as delta opioid receptor/OPRD1, beta-2 adrenergic receptor/ADRB2 and D4 dopamine receptor/DRD4 as well as D2 dopamine receptor/DRD2. Interacts with PER1 By similarity. Interacts with BECN1P1/BECN2; the interaction is direct. Ref.6

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed in the brain, with higher expression in the hippocampus, hypothalamus and olfactory bulb. Ref.5

Sequence similarities

Belongs to the GPRASP family.

Sequence caution

The sequence BAC41425.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

endosome to lysosome transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5U4C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5U4C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-473: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13471347G-protein coupled receptor-associated sorting protein 1
PRO_0000239051

Regions

Compositional bias1109 – 11124Poly-Leu

Natural variations

Alternative sequence1 – 473473Missing in isoform 2.
VSP_019080

Experimental info

Sequence conflict9421V → L in BAC41425. Ref.1
Sequence conflict9431I → F in BAC30259. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 1FD29E59CBF91B59

FASTA1,347151,745
        10         20         30         40         50         60 
MTRAEVEPGA QAKAENKPGD ENANAAEVEP EAPLVVRPKV RTQIMTGARP KVKPKGTPGA 

        70         80         90        100        110        120 
RPKGETSTPG GAYAKCKPKA IPIARSKHDA QVWAPNKFRG ESMSKMGKQC QISAADPPLL 

       130        140        150        160        170        180 
SNDSGMVAQA KCLPVDRELA NMDTESIPKK ANSPAGFQPS YGSEEGTNMG SWYRARPVPK 

       190        200        210        220        230        240 
GEAYENSDFK WADKPSGSPS FWNRDEASTR FRPRKSMKAN NRFRHMAKQE ANTMPRHKNK 

       250        260        270        280        290        300 
QEFYNISSSD SEDESGKTPW FWPKDKTKVW SKPKEEPNSR SWFRSKKEVR VESTSGSECE 

       310        320        330        340        350        360 
NPTKSLFWSG EEAKSRSKPR ARKGVNMRAR QQAKREACSD AMSGAIDTNK KESWFLPEEK 

       370        380        390        400        410        420 
ANVFSKSKTK KEPRTRAVPK EEVKTKARAS TKQEARPEEE VLVGAWFWDT QESTMADRIS 

       430        440        450        460        470        480 
IKTTFVEEEP IVGDWFWSEE EASVDSETCH TSRPRAKEEQ VSSFCLGSGK KSSMESGPKA 

       490        500        510        520        530        540 
TSKSMPVAKE DEVVIGSWFW ADDEEINLQA DDESIFGSWF WGTGENSLRS VGVNCEKMPK 

       550        560        570        580        590        600 
AGEKEVTDSW FWAGDVNTEA EVEEQARSAS TKATIFVPWF WSEKQPNMDL GSEPCSDIMA 

       610        620        630        640        650        660 
GAEEEPIIGP WFWAKVDNSV EAEVNSKSSL EDEEEPIRSP WFGAREQTDM KYAAGIRYKP 

       670        680        690        700        710        720 
MAEAEDANKK SCVWAKEPCL YPTNRECLKS TLGEKEDTVD PWLWSNNYPR TKTITGSWLW 

       730        740        750        760        770        780 
AAEEGNIDDE TGEKIKLPTL EDNAFNSWFW KENEESIVEA PKREEFRPEA EEEDIIGSWF 

       790        800        810        820        830        840 
WAGDEDRFEP AAKINEENKI ASEDEDTVGS WFWGNEEASL EAVRRGTFES APGIKEEKVT 

       850        860        870        880        890        900 
GSWFWTDKAK VGAGSQTVET GSETEEEAIF ESLIWAAKKD SIQAGVKRVS KPKDDGNIAV 

       910        920        930        940        950        960 
GSWLWSSDKA TKEAKTLIVS EASPENGKES VVKFGSRAKD EVINKTGSGD NCKHSTEAET 

       970        980        990       1000       1010       1020 
IVGAWFWEGD EASFESNPVP VCKAVCEPES SAEHEPDPSR RPQSWDEVTV QFKAGPWGKA 

      1030       1040       1050       1060       1070       1080 
GFPPMNPFRF PKEAASLFAE MFGGKPKLVE VGPEREPEPQ FPFQYDPSYR SVREIREHLK 

      1090       1100       1110       1120       1130       1140 
ARESAQPENW SCNCIQCELR IGSEEFEELL LLMDRNRDPF IHEISKIAMG MRGASQFTRD 

      1150       1160       1170       1180       1190       1200 
FIRNSGVVSL IEALLNYPSS RVRTRFLENM VRMAPPYPDL NMIETYVCQI CEDTFDYDLD 

      1210       1220       1230       1240       1250       1260 
SPDQLSGLTM ITHLTATSDY HKVVVNYLAG FFYLLNSGNT KTRFHVLKLL LNLSENLVMT 

      1270       1280       1290       1300       1310       1320 
KRLLVTDSVS EFMDLINREE SDENIQIVLA IFETISKHIQ KEALFSDDDD DDEEEDAVNL 

      1330       1340 
EPFISAFREA EKIAKELKRK PGNQKAP 

« Hide

Isoform 2 [UniParc].

Checksum: 403D64EEC544823D
Show »

FASTA87498,825

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain and Eye.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-379 AND 943-1347 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Embryo, Hippocampus and Hypothalamus.
[5]"Identification of a novel family of G protein-coupled receptor associated sorting proteins."
Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism."
He C., Wei Y., Sun K., Li B., Dong X., Zou Z., Liu Y., Kinch L.N., Khan S., Sinha S., Xavier R.J., Grishin N.V., Xiao G., Eskelinen E.L., Scherer P.E., Whistler J.L., Levine B.
Cell 154:1085-1099(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BECN1P1/BECN2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB093241 mRNA. Translation: BAC41425.1. Different initiation.
AL683822 Genomic DNA. Translation: CAM27375.1.
BC027187 mRNA. Translation: AAH27187.1.
BC085157 mRNA. Translation: AAH85157.1.
AK039156 mRNA. Translation: BAC30259.1.
AK050919 mRNA. Translation: BAC34459.2.
AK083192 mRNA. Translation: BAC38803.1.
RefSeqNP_001004359.1. NM_001004359.2.
NP_001005385.1. NM_001005385.1.
NP_080357.4. NM_026081.5.
UniGeneMm.271980.

3D structure databases

ProteinModelPortalQ5U4C1.
SMRQ5U4C1. Positions 1108-1160.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ5U4C1. 1 interaction.

PTM databases

PhosphoSiteQ5U4C1.

Proteomic databases

PaxDbQ5U4C1.
PRIDEQ5U4C1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000113144; ENSMUSP00000108769; ENSMUSG00000043384. [Q5U4C1-1]
ENSMUST00000113145; ENSMUSP00000108770; ENSMUSG00000043384. [Q5U4C1-1]
ENSMUST00000113147; ENSMUSP00000108772; ENSMUSG00000043384. [Q5U4C1-1]
ENSMUST00000166554; ENSMUSP00000132225; ENSMUSG00000043384. [Q5U4C1-1]
GeneID67298.
KEGGmmu:67298.
UCSCuc009uhn.1. mouse. [Q5U4C1-1]

Organism-specific databases

CTD9737.
MGIMGI:1917418. Gprasp1.
RougeSearch...

Phylogenomic databases

eggNOGNOG136667.
GeneTreeENSGT00550000074488.
HOVERGENHBG057167.
InParanoidA2AGB6.
OMAQADDESI.
OrthoDBEOG70PBZ0.
PhylomeDBQ5U4C1.
TreeFamTF335652.

Gene expression databases

BgeeQ5U4C1.
GenevestigatorQ5U4C1.

Family and domain databases

InterProIPR006911. ARM-rpt_dom.
IPR016024. ARM-type_fold.
[Graphical view]
PfamPF04826. Arm_2. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSGPRASP1. mouse.
NextBio324156.
PROQ5U4C1.
SOURCESearch...

Entry information

Entry nameGASP1_MOUSE
AccessionPrimary (citable) accession number: Q5U4C1
Secondary accession number(s): A2AGB6 expand/collapse secondary AC list , Q8BKR8, Q8BUN4, Q8BYK9, Q8CHF4, Q8R095
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot