Q5U3Z7 (Q5U3Z7_RAT) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Serine hydroxymethyltransferase RuleBase RU000585
EC=2.1.2.1 RuleBase RU000585

Gene names

Name:

Shmt2 EMBL AAH85331.1 Ensembl ENSRNOP00000011082 RGD 1308582

Organism

Rattus norvegicus (Rat) EMBL AAH85331.1

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Interconversion of serine and glycine By similarity. RuleBase RU000585
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. RuleBase RU000585
Cofactor	Pyridoxal phosphate By similarity. RuleBase RU000585
Pathway	One-carbon metabolism; tetrahydrofolate pathway. RuleBase RU000585
Sequence similarities	Belongs to the SHMT family. RuleBase RU004104

Ontologies

Keywords
Biological process	One-carbon metabolism RuleBase RU000585
Ligand	Pyridoxal phosphate RuleBase RU000585
Molecular function	Methyltransferase EMBL AAH85331.1 Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from direct assay. Source: RGD glycine biosynthetic process from serine Inferred from direct assay. Source: RGD positive regulation of cell proliferation Inferred from direct assay. Source: RGD protein homotetramerization Inferred from direct assay. Source: RGD
Cellular component	mitochondrial inner membrane Inferred from direct assay. Source: RGD mitochondrial intermembrane space Inferred from direct assay. Source: RGD
Molecular function	L-allo-threonine aldolase activity Inferred from direct assay. Source: RGD amino acid binding Inferred from physical interaction. Source: RGD glycine hydroxymethyltransferase activity Inferred from direct assay. Source: RGD identical protein binding Inferred from direct assay. Source: RGD methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5U3Z7 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 7A0FE0B01FC591A2	FASTA	504	55,765
	10 20 30 40 50 60 MVPFSLLRTT RPLQRCGQLV CMAARAQHSE VAQKQAGEAA GGWTGQESLS DSDPEIWELL 70 80 90 100 110 120 QREKDRQCRG LELIASENFC SRAALEALGS CLNNKYSEGY PGKRYYGGAE VVDEIELLCQ 130 140 150 160 170 180 RRALEAFDLD PAQWGVNVQP YSGSPANLAA YTALLQPHDR IMGLDLPDGG HLTHGYMSDV 190 200 210 220 230 240 KRISATSIFF ESMPYKLNPQ TGLIDYDQLA LTARLFRPRL IIAGTSAYAR LIDYARMREV 250 260 270 280 290 300 CDEVKAHLLA DMAHISGLVA AKVIPSPFKY ADIVTTTTHK TLRGARSGLI FYRKGVRTVD 310 320 330 340 350 360 PKTGQEIPYT FEDRINFAVF PSLQGGPHNH AIAAVAVALK QACTPMFREY SLQVLRNAQA 370 380 390 400 410 420 MADALLKRGY SLVSGGTDTH LVLVDLRPKG LDGARAERVL ELVSITANKN TCPGDRSAIT 430 440 450 460 470 480 PGGLRLGAPA LTSRQFREDD FRRVVDFIDE GVNIGLEVKR KTAKLQDFKS FLLKDPETSQ 490 500 RLANLRQQVE QFARGFPMPG FDER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney EMBL AAH85331.1.
[2]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Rat Genome Sequencing Project Consortium Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway Ensembl ENSRNOP00000011082.
[3]	Ensembl Submitted (JUL-2011) to UniProtKB Cited for: IDENTIFICATION. Strain: Brown Norway Ensembl ENSRNOP00000011082.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC085331 mRNA. Translation: AAH85331.1.
IPI	IPI00195109.
RefSeq	NP_001008323.1. NM_001008322.1.
UniGene	Rn.228322.
3D structure databases
ProteinModelPortal	Q5U3Z7.
SMR	Q5U3Z7. Positions 42-503.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5U3Z7. 1 interaction.
STRING	Q5U3Z7.
Proteomic databases
PRIDE	Q5U3Z7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011082; ENSRNOP00000011082; ENSRNOG00000008106.
GeneID	299857.
KEGG	rno:299857.
NMPDR	fig|10116.3.peg.26907.
UCSC	NM_001008322. rat.
Organism-specific databases
CTD	6472.
RGD	1308582. Shmt2.
Phylogenomic databases
eggNOG	roNOG05069.
GeneTree	ENSGT00390000002762.
HOVERGEN	HBG002807.
InParanoid	Q5U3Z7.
OMA	DYARIKK.
OrthoDB	EOG4G4GQ7.
PhylomeDB	Q5U3Z7.
Gene expression databases
ArrayExpress	Q5U3Z7.
Genevestigator	Q5U3Z7.
Family and domain databases
HAMAP	MF_00051. SHMT. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00600.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	645904.

Entry information

Entry name

Q5U3Z7_RAT

Accession

Primary (citable) accession number: Q5U3Z7

Entry history

Integrated into UniProtKB/TrEMBL:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)