Reviewed, UniProtKB/Swiss-Prot Q5U3T0 (MGT4C_DANRE)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc
      Short name=N-acetylglucosaminyltransferase IVc
      Short name=GnT-IVc

Gene names

Name:	mgat4c
ORF Names:	zgc:101663

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C		PRO_0000288601
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 24	18	Signal-anchor for type II membrane protein Potential
	Topological domain	25 – 454	430	Lumenal Potential
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5U3T0-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: E22350447B8D300A	FASTA	454	52,254
	10 20 30 40 50 60 MRCHLKKWVV VAAGLSILTS LYVYMQRAQS GNLKGSVIWT WDRESWMEGI KSHYLHFNIS 70 80 90 100 110 120 INVLGGVLQP SKKYLTVGLS SVRREKGFYL HDTLQSIFSE SSEEELDQMV VVVLLADFDL 130 140 150 160 170 180 PWIQQTADKI SREFAVQLSK GRLLVIHANK EHYPPLTGLK RNFNDAPDRV SFRSKQNVDY 190 200 210 220 230 240 SFLLHFSSNL SQYYIMLEDD VGCSRNFLSS IQQHIRSMTD SKWVTLEFSK LGYIGKLYQS 250 260 270 280 290 300 KDLPTLARFL YNFYQEMPCD FLLSHFRRLL MQDKVIRFRP SLFQHMGTYS SFRGTYNRLK 310 320 330 340 350 360 DEDFVQELAD NPPADVRTNI QVFQTYVPEK AYSQDVEYFW GVSPIGTESF FLVVFREAVR 370 380 390 400 410 420 ISRVQIHTGS DGKDELASAD VELGRVLVTG EPAVDCSGFK TLGSLQHGQF NEEGVQKLLP 430 440 450 DAVVCLRIRV TAAQPEWVII SRIQVWTVKE DKTI

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC085406 mRNA. Translation: AAH85406.1.
IPI	IPI00487920.
RefSeq	NP_001007438.1.
UniGene	Dr.77353
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Genome annotation databases
Ensembl	ENSDART00000055148; ENSDARP00000055147; ENSDARG00000037852; Danio rerio. [Genome view]
GeneID	492796.
KEGG	dre:492796.
Organism-specific databases
ZFIN	ZDB-GENE-041114-149. zgc:101663.
Phylogenomic databases
eggNOG	fiNOG15112.
HOGENOM	HBG444742.
HOVERGEN	Q5U3T0.
InParanoid	Q5U3T0.
OMA	QSEWLII.
OrthoDB	EOG9VDSHX.
PhylomeDB	Q5U3T0.
Enzyme and pathway databases
BRENDA	2.4.1.145. 96826.
Gene expression databases
ArrayExpress	Q5U3T0.
Bgee	Q5U3T0.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MGT4C_DANRE

Accession

Primary (citable) accession number: Q5U3T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 7, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project