Reviewed, UniProtKB/Swiss-Prot Q5U3F8 (3HAO_DANRE)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Name:	haao
ORF Names:	zgc:103585

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.
Cofactor	Fe2+ ion By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	3-hydroxyanthranilate 3,4-dioxygenase		PRO_0000245466
Sites
	Metal binding	50	1	Iron; catalytic By similarity
	Metal binding	56	1	Iron; catalytic By similarity
	Metal binding	94	1	Iron; catalytic By similarity
	Binding site	46	1	Dioxygen By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5U3F8-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: A5A2B050B823F038	FASTA	287	33,239
	10 20 30 40 50 60 MTNQSLHVNI DKWIAENETS FLPPVCNKLM FFYQLNIMYV GGPNVRKDYH IEEGEELFYQ 70 80 90 100 110 120 VRGDMVLKVI ENGKHKDVHI REGEMFLLPA RIPHSPQRQA NTVGLVIERR RLSKETDGLR 130 140 150 160 170 180 YFVANSTEVL FERWFYCENL GTQLVPIIKE FMDSKENETG KPDPANPIKP APYPLNTMNV 190 200 210 220 230 240 MTPFSFREWV EKQKPVLASG CPVDMFGEQF ETETLLFGSG TSANKRRTDG WIWQLEGLSN 250 260 270 280 VFMNGKEYSL TAGDCLLIFG ETEYKWQRSQ DCVALYVAQD PDRKRPY

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC085560 mRNA. Translation: AAH85560.1.
IPI	IPI00509740.
RefSeq	NP_001007391.1.
UniGene	Dr.37102
3D structure databases
SMR	Q5U3F8. Positions 6-287.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000100241; ENSDARP00000091014; ENSDARG00000076487; Danio rerio. [Genome view]
GeneID	492518.
KEGG	dre:492518.
Organism-specific databases
ZFIN	ZDB-GENE-041114-89. zgc:103585.
Phylogenomic databases
HOVERGEN	Q5U3F8.
PhylomeDB	Q5U3F8.
Enzyme and pathway databases
BRENDA	1.13.11.6. 96826.
Gene expression databases
Bgee	Q5U3F8.
Family and domain databases
InterPro	IPR010329. 3hydroanth_dOase. IPR016700. 3hydroanth_dOase_met. IPR011051. Cupin_RmlC_type. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
PIRSF	PIRSF017681. 3hydroanth_dOase_animal. 1 hit.
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_DANRE

Accession

Primary (citable) accession number: Q5U3F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	December 7, 2004
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project