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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

haao

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathway:iNAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (kmo), Kynurenine 3-monooxygenase (kmo), Kynurenine 3-monooxygenase (kmo)
  2. no protein annotated in this organism
  3. 3-hydroxyanthranilate 3,4-dioxygenase (haao), 3-hydroxyanthranilate 3,4-dioxygenase (haao), 3-hydroxyanthranilate 3,4-dioxygenase (haao)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461DioxygenUniRule annotation
Metal bindingi50 – 501Iron; catalyticUniRule annotation
Metal bindingi56 – 561Iron; catalyticUniRule annotation
Binding sitei56 – 561SubstrateUniRule annotation
Metal bindingi94 – 941Iron; catalyticUniRule annotation
Binding sitei98 – 981SubstrateUniRule annotation
Binding sitei108 – 1081SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:haao
ORF Names:zgc:103585
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-041114-89. haao.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2872873-hydroxyanthranilate 3,4-dioxygenasePRO_0000245466Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091014.

Structurei

3D structure databases

ProteinModelPortaliQ5U3F8.
SMRiQ5U3F8. Positions 6-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 163163Domain A (catalytic)UniRule annotationAdd
BLAST
Regioni164 – 18017LinkerUniRule annotationAdd
BLAST
Regioni181 – 287107Domain BUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiNOG77058.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ5U3F8.
KOiK00452.
PhylomeDBiQ5U3F8.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5U3F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNQSLHVNI DKWIAENETS FLPPVCNKLM FFYQLNIMYV GGPNVRKDYH
60 70 80 90 100
IEEGEELFYQ VRGDMVLKVI ENGKHKDVHI REGEMFLLPA RIPHSPQRQA
110 120 130 140 150
NTVGLVIERR RLSKETDGLR YFVANSTEVL FERWFYCENL GTQLVPIIKE
160 170 180 190 200
FMDSKENETG KPDPANPIKP APYPLNTMNV MTPFSFREWV EKQKPVLASG
210 220 230 240 250
CPVDMFGEQF ETETLLFGSG TSANKRRTDG WIWQLEGLSN VFMNGKEYSL
260 270 280
TAGDCLLIFG ETEYKWQRSQ DCVALYVAQD PDRKRPY
Length:287
Mass (Da):33,239
Last modified:December 7, 2004 - v1
Checksum:iA5A2B050B823F038
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085560 mRNA. Translation: AAH85560.1.
RefSeqiNP_001007391.1. NM_001007390.1.
UniGeneiDr.37102.

Genome annotation databases

GeneIDi492518.
KEGGidre:492518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085560 mRNA. Translation: AAH85560.1.
RefSeqiNP_001007391.1. NM_001007390.1.
UniGeneiDr.37102.

3D structure databases

ProteinModelPortaliQ5U3F8.
SMRiQ5U3F8. Positions 6-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi492518.
KEGGidre:492518.

Organism-specific databases

CTDi23498.
ZFINiZDB-GENE-041114-89. haao.

Phylogenomic databases

eggNOGiNOG77058.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ5U3F8.
KOiK00452.
PhylomeDBiQ5U3F8.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.

Miscellaneous databases

NextBioi20865078.
PROiQ5U3F8.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry namei3HAO_DANRE
AccessioniPrimary (citable) accession number: Q5U3F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 7, 2004
Last modified: April 29, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.