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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

Gene

Plod3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.By similarity

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi670IronPROSITE-ProRule annotation1
Metal bindingi672IronPROSITE-ProRule annotation1
Metal bindingi722IronPROSITE-ProRule annotation1
Active sitei732Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiR-RNO-1650814 Collagen biosynthesis and modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (EC:1.14.11.4By similarity)
Alternative name(s):
Lysyl hydroxylase 3
Short name:
LH3
Gene namesi
Name:Plod3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi631339 Plod3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000004177428 – 741Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5U367
PRIDEiQ5U367

Expressioni

Gene expression databases

BgeeiENSRNOG00000001417
GenevisibleiQ5U367 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ5U367, 1 interactor
STRINGi10116.ENSRNOP00000001921

Structurei

3D structure databases

ProteinModelPortaliQ5U367
SMRiQ5U367
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini650 – 741Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST92

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971 Eukaryota
ENOG410Y4QU LUCA
GeneTreeiENSGT00550000074427
HOGENOMiHOG000231099
HOVERGENiHBG053618
InParanoidiQ5U367
KOiK13646
OMAiLFYTRLY
OrthoDBiEOG091G02DK
PhylomeDBiQ5U367
TreeFamiTF313826

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR006620 Pro_4_hyd_alph
IPR001006 Procol_lys_dOase
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit
PS01325 LYS_HYDROXYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVPEPRL LLLLLLLLPP LPPVTSASDR PRGANPVNPD KLLVITVATA
60 70 80 90 100
ETEGYRRFLQ SAEFFNYTVR TLGLGQEWRG GDVARTVGGG QKVRWLKKEM
110 120 130 140 150
EKYASQEDMI IMFVDSYDVI LASSPTELLK KFVQSGSHLL FSAESFCWPD
160 170 180 190 200
WGLAEQYPEV GVGKRFLNSG GFIGFAPTIH RIVRQWKYKD DDDDQLFYTQ
210 220 230 240 250
LYLDPGLREK LKLSLDHKSR IFQNLNGALD EVVLKFDQNR VRIRNVAYDT
260 270 280 290 300
LPVVVHGNGP TKLQLNYLGN YVPNGWTPQG GCGFCNLNRR TLPGGQPPPR
310 320 330 340 350
VLLAVFVEQP TPFLPRFLQR LLLLDYPPDR ISLFLHNNEV YHEPHIADAW
360 370 380 390 400
PQLQDHFSAV KLVGPEEALS SGEARDMAMD SCRQNPECEF YFSLDADAVL
410 420 430 440 450
TNPETLRILI EQNRKVIAPM LSRHGKLWSN FWGALSPDEY YARSEDYVEL
460 470 480 490 500
VQRKRVGLWN VPYISQAYVI RGETLRTELP EKEVFSSSDT DPDMAFCRSV
510 520 530 540 550
RDKGIFLHLS NQHEFGRLLS TSHYDTDHLH PDLWQIFDNP VDWREQYIHE
560 570 580 590 600
NYSRALDGEG LVEQPCPDVY WFPLLTEQMC DELVEEMEHY GQWSGGRHED
610 620 630 640 650
SRLAGGYENV PTVDIHMKQV GYEDQWLQLL RTYVGPMTEH LFPGYHTKTR
660 670 680 690 700
AVMNFVVRYR PDEQPSLRPH HDSSTFTLNV ALNHKGVDYE GGGCRFLRYD
710 720 730 740
CRVSSPRKGW ALLHPGRLTH YHEGLPTTRG TRYIMVSFVD P
Length:741
Mass (Da):85,060
Last modified:December 7, 2004 - v1
Checksum:iE14883A4D2522767
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 10SVPEPRL → ISPGTPA in CAD23628 (PubMed:12878181).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ430859 mRNA Translation: CAD23628.1
BC085683 mRNA Translation: AAH85683.1
RefSeqiNP_835202.2, NM_178101.3
UniGeneiRn.90152

Genome annotation databases

EnsembliENSRNOT00000001921; ENSRNOP00000001921; ENSRNOG00000001417
GeneIDi288583
KEGGirno:288583
UCSCiRGD:631339 rat

Similar proteinsi

Entry informationi

Entry nameiPLOD3_RAT
AccessioniPrimary (citable) accession number: Q5U367
Secondary accession number(s): Q811A5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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