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Q5U316

- RAB35_RAT

UniProt

Q5U316 - RAB35_RAT

Protein

Ras-related protein Rab-35

Gene

Rab35

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth By similarity.By similarity

    Enzyme regulationi

    Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the guanine exchange factors DENND1A, DENND1B and DENND1C By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi120 – 1234GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTP binding Source: UniProtKB
    3. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl

    GO - Biological processi

    1. cellular response to nerve growth factor stimulus Source: UniProtKB
    2. cytokinesis Source: Ensembl
    3. endosomal transport Source: UniProtKB
    4. neuron projection development Source: UniProtKB
    5. protein localization to endosome Source: UniProtKB
    6. protein transport Source: UniProtKB-KW
    7. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-35
    Gene namesi
    Name:Rab35
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi1306362. Rab35.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Membraneclathrin-coated pit By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Endosome 1 Publication. Melanosome By similarity
    Note: Present on sorting endosomes and recycling endosome tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified in stage I to stage IV melanosomes.

    GO - Cellular componenti

    1. cell projection membrane Source: Ensembl
    2. clathrin-coated endocytic vesicle Source: Ensembl
    3. coated pit Source: UniProtKB-SubCell
    4. endosome membrane Source: UniProtKB
    5. intercellular bridge Source: Ensembl
    6. melanosome Source: UniProtKB-SubCell
    7. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Coated pit, Cytoplasmic vesicle, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 201201Ras-related protein Rab-35PRO_0000121247Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity
    Lipidationi201 – 2011S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    PaxDbiQ5U316.
    PRIDEiQ5U316.

    PTM databases

    PhosphoSiteiQ5U316.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5U316.

    Interactioni

    Subunit structurei

    Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner. Interacts with DENND1C; weak interaction which is nucleotide-independent. Interacts (GTP-bound form) with ACAP2 and MICALL1; the interaction is direct and probably recruits ACAP2 and MICALL1 to membranes. Interacts with EHD1; the interaction is indirect through MICALL1 and probably recruits EHD1 to membranes.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000029070.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U316.
    SMRiQ5U316. Positions 4-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000115017.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiQ5U316.
    KOiK07876.
    OMAiNRILVGN.
    OrthoDBiEOG7VB2H4.
    PhylomeDBiQ5U316.
    TreeFamiTF105954.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5U316-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV    50
    EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK 100
    RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS 150
    AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC 200
    C 201
    Length:201
    Mass (Da):23,025
    Last modified:December 7, 2004 - v1
    Checksum:i31EB15D6D42E076E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC085769 mRNA. Translation: AAH85769.1.
    RefSeqiNP_001013064.1. NM_001013046.1.
    UniGeneiRn.18008.

    Genome annotation databases

    EnsembliENSRNOT00000030031; ENSRNOP00000029070; ENSRNOG00000022014.
    GeneIDi288700.
    KEGGirno:288700.
    UCSCiRGD:1306362. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC085769 mRNA. Translation: AAH85769.1 .
    RefSeqi NP_001013064.1. NM_001013046.1.
    UniGenei Rn.18008.

    3D structure databases

    ProteinModelPortali Q5U316.
    SMRi Q5U316. Positions 4-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000029070.

    PTM databases

    PhosphoSitei Q5U316.

    Proteomic databases

    PaxDbi Q5U316.
    PRIDEi Q5U316.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000030031 ; ENSRNOP00000029070 ; ENSRNOG00000022014 .
    GeneIDi 288700.
    KEGGi rno:288700.
    UCSCi RGD:1306362. rat.

    Organism-specific databases

    CTDi 11021.
    RGDi 1306362. Rab35.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000115017.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi Q5U316.
    KOi K07876.
    OMAi NRILVGN.
    OrthoDBi EOG7VB2H4.
    PhylomeDBi Q5U316.
    TreeFami TF105954.

    Miscellaneous databases

    NextBioi 628573.
    PROi Q5U316.

    Gene expression databases

    Genevestigatori Q5U316.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
      Kobayashi H., Fukuda M.
      J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ACAP2; EHD1 AND MICALL1, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiRAB35_RAT
    AccessioniPrimary (citable) accession number: Q5U316
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3