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Protein

Poly(A) RNA polymerase GLD2

Gene

Papd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. hematopoietic progenitor cell differentiation Source: Ensembl
  2. histone mRNA catabolic process Source: UniProtKB
  3. mRNA processing Source: UniProtKB-KW
  4. RNA polyadenylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Alternative name(s):
PAP-associated domain-containing protein 4
Gene namesi
Name:Papd4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1306438. Papd4.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: RGD
  2. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341551Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U315.

Expressioni

Gene expression databases

GenevestigatoriQ5U315.

Interactioni

Subunit structurei

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016135.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 44055PAP-associatedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ5U315.
KOiK14079.
OMAiHHEINDA.
OrthoDBiEOG7DRJ2S.
PhylomeDBiQ5U315.
TreeFamiTF315661.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPNSILGRP PFTPTHQQHN NFFALSPTLY SHQQLIDAQF NFQNVDLSRA
60 70 80 90 100
VSLQPLTYGT VSPIQTSTSP LFRGRKRISD EKAFRLDGKR QRFHSPHQEP
110 120 130 140 150
TIINQLVPLS GDRRYSMPPL FHTHYVPEIV RCVPPLREIP LLEPREITLP
160 170 180 190 200
EAKDKLSQQI LELFETCQQQ ASDLKKKELC RAQLQREIQL LFPQSRLFLV
210 220 230 240 250
GSSLNGFGAR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL
260 270 280 290 300
SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNTVGIRN TFLLRTYAYL
310 320 330 340 350
ENRVRPLVLV IKKWASHHEI NDASRGTLSS YSLVLMVLHY LQTLPEPILP
360 370 380 390 400
SLQKIYPESF STSVQLHLVH HAPCNVPPYL SKNESSLGDL LLGFLKYYAT
410 420 430 440 450
EFDWNTQMIS VREAKAIPRP DDMEWRNKYI CVEEPFDGTN TARAVHEKQK
460 470 480
FDMIKDQFLK SWQRLKNKRD LNSVLPLRAA TLKR
Length:484
Mass (Da):56,007
Last modified:December 7, 2004 - v1
Checksum:i149E8B0C49142A60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085771 mRNA. Translation: AAH85771.1.
RefSeqiNP_001008373.1. NM_001008372.1.
XP_006231838.1. XM_006231776.2.
XP_008758861.1. XM_008760639.1.
UniGeneiRn.98477.

Genome annotation databases

EnsembliENSRNOT00000016135; ENSRNOP00000016135; ENSRNOG00000012099.
GeneIDi361878.
KEGGirno:361878.
UCSCiRGD:1306438. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085771 mRNA. Translation: AAH85771.1.
RefSeqiNP_001008373.1. NM_001008372.1.
XP_006231838.1. XM_006231776.2.
XP_008758861.1. XM_008760639.1.
UniGeneiRn.98477.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016135.

Proteomic databases

PaxDbiQ5U315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016135; ENSRNOP00000016135; ENSRNOG00000012099.
GeneIDi361878.
KEGGirno:361878.
UCSCiRGD:1306438. rat.

Organism-specific databases

CTDi167153.
RGDi1306438. Papd4.

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ5U315.
KOiK14079.
OMAiHHEINDA.
OrthoDBiEOG7DRJ2S.
PhylomeDBiQ5U315.
TreeFamiTF315661.

Miscellaneous databases

NextBioi677940.
PROiQ5U315.

Gene expression databases

GenevestigatoriQ5U315.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiGLD2_RAT
AccessioniPrimary (citable) accession number: Q5U315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 7, 2004
Last modified: April 1, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.