Ubiquitin-like modifier-activating enzyme 1

Contribute

Send feedback

Read comments (?) or add your own

Q5U300 (UBA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin-like modifier-activating enzyme 1

Alternative name(s):
Ubiquitin-activating enzyme E1

Gene names

Name:	Uba1
Synonyms:	Ube1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1058 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Monomer. Interacts with GAN (via BTB domain) By similarity. UniProtKB Q02053
Post-translational modification	ISGylated By similarity.
Miscellaneous	There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW small protein activating enzyme activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1058

1058

Ubiquitin-like modifier-activating enzyme 1

PRO_0000365096

Regions

Repeat

63 – 199

137

1-1

Repeat

459 – 611

153

1-2

Nucleotide binding

478 – 507

ATP By similarity UniProtKB Q02053

Region

63 – 611

549

2 approximate repeats

Sites

Active site

632

Glycyl thioester intermediate By similarity UniProtKB Q02053

Amino acid modifications

Modified residue

Phosphoserine By similarity UniProtKB Q02053

Modified residue

Phosphoserine By similarity UniProtKB Q02053

Modified residue

Phosphoserine By similarity UniProtKB Q02053

Modified residue

Phosphoserine By similarity UniProtKB Q02053

Modified residue

Phosphoserine By similarity UniProtKB Q02053

Modified residue

Phosphotyrosine By similarity UniProtKB Q02053

Modified residue

671

N6-acetyllysine By similarity

Modified residue

800

Phosphothreonine By similarity UniProtKB Q02053

Modified residue

810

Phosphoserine By similarity

Modified residue

816

Phosphoserine By similarity UniProtKB Q02053

Modified residue

820

Phosphoserine By similarity UniProtKB Q02053

Modified residue

835

Phosphoserine By similarity UniProtKB Q02053

Modified residue

980

N6-acetyllysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q5U300 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: FAC21190A3014F79
Show »

FASTA

1,058

117,788

        10         20         30         40         50         60 
MSSSPLSKKR RVSGPDPKPG SNCSSAQSVL SEVSSVPTNG MAKNGSEADI DESLYSRQLY 

        70         80         90        100        110        120 
VLGHEAMKML QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTTQWA DLSSQFYLRE 

       130        140        150        160        170        180 
EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSGFQ VVVLTNSPLE EQLRVGEFCH 

       190        200        210        220        230        240 
SRGIKLVVAD TRGLFGQLFC DFGEEMVLTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH 

       250        260        270        280        290        300 
GFETGDFVSF SEVQGMVQLN GCQPIEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK 

       310        320        330        340        350        360 
ISFKSLPASL AEPDFVMTDF AKYSRPAQLH IGFQALHQFC AQHNRPPRPR NEEDATELVT 

       370        380        390        400        410        420 
LAQAVNARSP PAVQQDNVDE DLIRKLAYVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI 

       430        440        450        460        470        480 
MQWLYFDALE CLPEDKEALT EDKCLPRQNR YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG 

       490        500        510        520        530        540 
CELLKNFAMI GLGCGEGGEV VVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN 

       550        560        570        580        590        600 
PYIQVTSHQN RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT 

       610        620        630        640        650        660 
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA 

       670        680        690        700        710        720 
ENVNQYLTDS KFVERTLRLA GTQPLEVLEA VQRSLVLQRP QTWGDCVTWA CHHWHTQYCN 

       730        740        750        760        770        780 
NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVNNTL HLDYVMAAAN LFAQTYGLTG 

       790        800        810        820        830        840 
SQDRAAVASL LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP 

       850        860        870        880        890        900 
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDISPADR HKSKLIAGKI IPAIATTTAA 

       910        920        930        940        950        960 
VVGLVCLELY KVVQGHQQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV 

       970        980        990       1000       1010       1020 
QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS 

      1030       1040       1050 
RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Brown Norway. Tissue: Kidney.
[3]	Maurya D.K., Bhargava P. Submitted (JAN-2009) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH474009 Genomic DNA. Translation: EDL97701.1. CH474009 Genomic DNA. Translation: EDL97702.1. BC085791 mRNA. Translation: AAH85791.1.
IPI	IPI00368347.
RefSeq	NP_001014102.1. NM_001014080.1.
UniGene	Rn.11800.
3D structure databases
HSSP	HSSP built from PDB template 1Z7L based on UniProtKB Q02053.
ProteinModelPortal	Q5U300.
SMR	Q5U300. Positions 48-1057.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000033950.
PTM databases
PhosphoSite	Q5U300.
Proteomic databases
PaxDb	Q5U300.
PRIDE	Q5U300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000031115; ENSRNOP00000033950; ENSRNOG00000019164.
GeneID	314432.
KEGG	rno:314432.
UCSC	RGD:1359327. rat.
Organism-specific databases
CTD	7317.
RGD	1359327. Uba1.
Phylogenomic databases
eggNOG	COG0476.
GeneTree	ENSGT00390000016689.
HOGENOM	HOG000167329.
HOVERGEN	HBG054199.
InParanoid	Q5U300.
KO	K03178.
OMA	FESGDYV.
OrthoDB	EOG4QZ7K4.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q5U300.
Family and domain databases
Gene3D	1.10.3240.10. 1 hit. 3.40.50.720. 4 hits.
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. IPR018965. Ub-activating_enz_e1_C. IPR023280. Ub-like_act_enz_cat_cys_dom. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR018074. UBQ-activ_enz_E1_AS. IPR000011. UBQ/SUMO-activ_enz_E1-like. [Graphical view]
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
SMART	SM00985. UBA_e1_C. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 2 hits.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. 1 hit. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	667673.

Entry information

Entry name

UBA1_RAT

Accession

Primary (citable) accession number: Q5U300

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents