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Q5U300

- UBA1_RAT

UniProt

Q5U300 - UBA1_RAT

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Protein

Ubiquitin-like modifier-activating enzyme 1

Gene

Uba1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei632 – 6321Glycyl thioester intermediateBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi478 – 50730ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: Ensembl
  3. ubiquitin activating enzyme activity Source: RefGenome
  4. ubiquitin-protein transferase activity Source: RefGenome

GO - Biological processi

  1. modification-dependent protein catabolic process Source: RefGenome
  2. protein ubiquitination Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 1By similarity
Alternative name(s):
Ubiquitin-activating enzyme E1By similarity
Gene namesi
Name:Uba1Imported
Synonyms:Ube1By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi1359327. Uba1.

Subcellular locationi

Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: RefGenome
  3. desmosome Source: Ensembl
  4. endosome membrane Source: Ensembl
  5. extracellular vesicular exosome Source: Ensembl
  6. heterochromatin Source: Ensembl
  7. lysosomal membrane Source: Ensembl
  8. mitochondrion Source: UniProtKB
  9. nucleus Source: UniProtKB
  10. rough endoplasmic reticulum membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 10581057Ubiquitin-like modifier-activating enzyme 1PRO_0000365096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei55 – 551PhosphotyrosineBy similarity
Modified residuei528 – 5281N6-succinyllysineBy similarity
Modified residuei671 – 6711N6-acetyllysineBy similarity
Modified residuei800 – 8001PhosphothreonineBy similarity
Modified residuei810 – 8101PhosphoserineBy similarity
Modified residuei816 – 8161PhosphoserineBy similarity
Modified residuei820 – 8201PhosphoserineBy similarity
Modified residuei835 – 8351PhosphoserineBy similarity
Modified residuei980 – 9801N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5U300.
PRIDEiQ5U300.

PTM databases

PhosphoSiteiQ5U300.

Expressioni

Gene expression databases

GenevestigatoriQ5U300.

Interactioni

Subunit structurei

Monomer. Interacts with GAN (via BTB domain) (By similarity).By similarity

Protein-protein interaction databases

BioGridi260716. 3 interactions.
STRINGi10116.ENSRNOP00000033950.

Structurei

3D structure databases

ProteinModelPortaliQ5U300.
SMRiQ5U300. Positions 48-1057.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati63 – 1991371-1Sequence AnalysisAdd
BLAST
Repeati459 – 6111531-2Sequence AnalysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 6115492 approximate repeatsSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Sequence Analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0476.
GeneTreeiENSGT00390000016689.
HOGENOMiHOG000167329.
HOVERGENiHBG054199.
InParanoidiQ5U300.
KOiK03178.
OMAiPFFAFSE.
OrthoDBiEOG74R1PV.
PhylomeDBiQ5U300.
TreeFamiTF300586.

Family and domain databases

Gene3Di1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProiIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U300-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSPLSKKR RVSGPDPKPG SNCSSAQSVL SEVSSVPTNG MAKNGSEADI
60 70 80 90 100
DESLYSRQLY VLGHEAMKML QTSSVLVSGL RGLGVEIAKN IILGGVKAVT
110 120 130 140 150
LHDQGTTQWA DLSSQFYLRE EDIGKNRAEV SQPRLAELNS YVPVTAYTGP
160 170 180 190 200
LVEDFLSGFQ VVVLTNSPLE EQLRVGEFCH SRGIKLVVAD TRGLFGQLFC
210 220 230 240 250
DFGEEMVLTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH GFETGDFVSF
260 270 280 290 300
SEVQGMVQLN GCQPIEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK
310 320 330 340 350
ISFKSLPASL AEPDFVMTDF AKYSRPAQLH IGFQALHQFC AQHNRPPRPR
360 370 380 390 400
NEEDATELVT LAQAVNARSP PAVQQDNVDE DLIRKLAYVA AGDLAPINAF
410 420 430 440 450
IGGLAAQEVM KACSGKFMPI MQWLYFDALE CLPEDKEALT EDKCLPRQNR
460 470 480 490 500
YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG CELLKNFAMI GLGCGEGGEV
510 520 530 540 550
VVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN PYIQVTSHQN
560 570 580 590 600
RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT
610 620 630 640 650
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD
660 670 680 690 700
EFEGLFKQPA ENVNQYLTDS KFVERTLRLA GTQPLEVLEA VQRSLVLQRP
710 720 730 740 750
QTWGDCVTWA CHHWHTQYCN NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH
760 770 780 790 800
PLTFDVNNTL HLDYVMAAAN LFAQTYGLTG SQDRAAVASL LQSVQVPEFT
810 820 830 840 850
PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP GFKMYPIDFE
860 870 880 890 900
KDDDSNFHMD FIVAASNLRA ENYDISPADR HKSKLIAGKI IPAIATTTAA
910 920 930 940 950
VVGLVCLELY KVVQGHQQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ
960 970 980 990 1000
EWTLWDRFEV QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF
1010 1020 1030 1040 1050
FMPAAKLKER LDQPMTEIVS RVSKRKLGRH VRALVLELCC NDESGEDVEV

PYVRYTIR
Length:1,058
Mass (Da):117,788
Last modified:December 7, 2004 - v1
Checksum:iFAC21190A3014F79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474009 Genomic DNA. Translation: EDL97701.1.
CH474009 Genomic DNA. Translation: EDL97702.1.
BC085791 mRNA. Translation: AAH85791.1.
RefSeqiNP_001014102.1. NM_001014080.1.
XP_006256674.1. XM_006256612.2.
UniGeneiRn.11800.

Genome annotation databases

EnsembliENSRNOT00000031115; ENSRNOP00000033950; ENSRNOG00000019164.
GeneIDi314432.
KEGGirno:314432.
UCSCiRGD:1359327. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474009 Genomic DNA. Translation: EDL97701.1 .
CH474009 Genomic DNA. Translation: EDL97702.1 .
BC085791 mRNA. Translation: AAH85791.1 .
RefSeqi NP_001014102.1. NM_001014080.1.
XP_006256674.1. XM_006256612.2.
UniGenei Rn.11800.

3D structure databases

ProteinModelPortali Q5U300.
SMRi Q5U300. Positions 48-1057.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 260716. 3 interactions.
STRINGi 10116.ENSRNOP00000033950.

PTM databases

PhosphoSitei Q5U300.

Proteomic databases

PaxDbi Q5U300.
PRIDEi Q5U300.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000031115 ; ENSRNOP00000033950 ; ENSRNOG00000019164 .
GeneIDi 314432.
KEGGi rno:314432.
UCSCi RGD:1359327. rat.

Organism-specific databases

CTDi 7317.
RGDi 1359327. Uba1.

Phylogenomic databases

eggNOGi COG0476.
GeneTreei ENSGT00390000016689.
HOGENOMi HOG000167329.
HOVERGENi HBG054199.
InParanoidi Q5U300.
KOi K03178.
OMAi PFFAFSE.
OrthoDBi EOG74R1PV.
PhylomeDBi Q5U300.
TreeFami TF300586.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi 667673.
PROi Q5U300.

Gene expression databases

Genevestigatori Q5U300.

Family and domain databases

Gene3Di 1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProi IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view ]
Pfami PF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view ]
PRINTSi PR01849. UBIQUITINACT.
SMARTi SM00985. UBA_e1_C. 1 hit.
[Graphical view ]
SUPFAMi SSF69572. SSF69572. 2 hits.
TIGRFAMsi TIGR01408. Ube1. 1 hit.
PROSITEi PS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway1 Publication.
    Tissue: KidneyImported.
  3. Maurya D.K., Bhargava P.
    Submitted (JAN-2009) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiUBA1_RAT
AccessioniPrimary (citable) accession number: Q5U300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: December 7, 2004
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3