Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

Cmtr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m7GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.By similarity

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei280 – 2801S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei310 – 3101S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei363 – 3631S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei403 – 4031Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
Alternative name(s):
Cap methyltransferase 1
Cap1 2'O-ribose methyltransferase 1
Short name:
MTr1
FtsJ methyltransferase domain-containing protein 2
Gene namesi
Name:Cmtr1
Synonyms:Ftsjd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1307801. Cmtr1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 837837Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1PRO_0000251242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei30 – 301PhosphoserineBy similarity
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei107 – 1071N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5U2Z5.
PRIDEiQ5U2Z5.

PTM databases

iPTMnetiQ5U2Z5.
PhosphoSiteiQ5U2Z5.

Expressioni

Gene expression databases

ExpressionAtlasiQ5U2Z5. baseline and differential.
GenevisibleiQ5U2Z5. RN.

Interactioni

Subunit structurei

Interacts with POLR2A (via C-terminus).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5U2Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 13247G-patchPROSITE-ProRule annotationAdd
BLAST
Domaini230 – 449220RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
BLAST
Domaini751 – 78535WWAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni726 – 834109Interaction with POLR2ABy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 G-patch domain.PROSITE-ProRule annotation
Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
Contains 1 WW domain.Curated

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
GeneTreeiENSGT00530000063742.
HOVERGENiHBG057271.
InParanoidiQ5U2Z5.
KOiK14589.
OMAiSRLFWEW.
OrthoDBiEOG78D7JK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030376. Cap_mRNA_MeTrfase_1.
IPR000467. G_patch_dom.
IPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR16121:SF0. PTHR16121:SF0. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
PF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51613. SAM_MT_RRMJ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRTDPECT APLKKQKRIG ELARHLSSTS DDEPLSSVSH AAKASTTSLS
60 70 80 90 100
GSDSETEGKQ PCSDGFKDAF KADSLVEGTS SRYSMYNSVS QKLMAKMGFR
110 120 130 140 150
EGEGLGKYSQ GRKDIVETSN QKGRRGLGLT LQGFDQELNV DWRDEPEPNA
160 170 180 190 200
CEQVSWFPEC TTEIPDSREM SDWMVVGKRK MVIEDETEFC GEELLHSMLK
210 220 230 240 250
CKSVFDILDG EEMRRARTRA NPYEMIRGVF FLNRAAMKMA NMDFVFDRMF
260 270 280 290 300
TNPLDSSGKP LLKESDIDLL YFADVCAGPG GFSEYVLWRR KWHAKGFGMT
310 320 330 340 350
LKGPNDFKLE DFYSASSELF EPYYGEGGVD GDGDITRPEN INAFRNFVLD
360 370 380 390 400
NTDRKGVHFV MADGGFSVEG QENLQEILSK QLLLCQFLMA LSVVRTGGHF
410 420 430 440 450
VCKTFDLFTP FSVGLIYLLY CCFERVCLFK PVTSRPANSE RYVVCKGLKV
460 470 480 490 500
GIDDVREYLF SVNIQLNQLR NTDSDVNLVV PLSVIKGDHE FNDYMIRSNE
510 520 530 540 550
SYCSLQIKAL AKIHAFVQDT TLSEPRQAEI RKECLQLWEI PDRARVAPSP
560 570 580 590 600
SDPKFKFFEL IKDTDINIFS YKPTLLTAKT LEKIRPVLEY RCMVSGSEQK
610 620 630 640 650
FLLGLGKSQI YTWDGRQSDR WVKLDLKTEL PRDTLLCVEI VHELKGEGKA
660 670 680 690 700
QRKISAIHIL DVLVLNGSDV REQHFNQRIQ LAEKFVKAVS KPSRPDMNPI
710 720 730 740 750
RVKEVYRLEE MEKIFVRLEM KLIKGSGGTP KLSYTGRDDR HFVPTGVYIV
760 770 780 790 800
RTVTEPWTMA FSKGWKKKFF YNKKTGESFF TLPPESIAPF HTCYYTRLFW
810 820 830
EWGDGFHMRD SQKPQDPDKL SKEDVLSFIQ SHNPLGP
Length:837
Mass (Da):95,619
Last modified:December 7, 2004 - v1
Checksum:i443BFE2882E687CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085799 mRNA. Translation: AAH85799.1.
RefSeqiNP_001014053.1. NM_001014031.1.
UniGeneiRn.17113.

Genome annotation databases

EnsembliENSRNOT00000000640; ENSRNOP00000000640; ENSRNOG00000000532.
GeneIDi309656.
KEGGirno:309656.
UCSCiRGD:1307801. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085799 mRNA. Translation: AAH85799.1.
RefSeqiNP_001014053.1. NM_001014031.1.
UniGeneiRn.17113.

3D structure databases

ProteinModelPortaliQ5U2Z5.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ5U2Z5.
PhosphoSiteiQ5U2Z5.

Proteomic databases

PaxDbiQ5U2Z5.
PRIDEiQ5U2Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000640; ENSRNOP00000000640; ENSRNOG00000000532.
GeneIDi309656.
KEGGirno:309656.
UCSCiRGD:1307801. rat.

Organism-specific databases

CTDi23070.
RGDi1307801. Cmtr1.

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
GeneTreeiENSGT00530000063742.
HOVERGENiHBG057271.
InParanoidiQ5U2Z5.
KOiK14589.
OMAiSRLFWEW.
OrthoDBiEOG78D7JK.

Miscellaneous databases

PROiQ5U2Z5.

Gene expression databases

ExpressionAtlasiQ5U2Z5. baseline and differential.
GenevisibleiQ5U2Z5. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030376. Cap_mRNA_MeTrfase_1.
IPR000467. G_patch_dom.
IPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR16121:SF0. PTHR16121:SF0. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
PF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51613. SAM_MT_RRMJ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Kidney.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCMTR1_RAT
AccessioniPrimary (citable) accession number: Q5U2Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.