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Q5U2Z5 (MTR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
EC=2.1.1.57
Alternative name(s):
Cap1 2'O-ribose methyltransferase 1
Short name=MTr1
FtsJ methyltransferase domain-containing protein 2
Gene names
Name:Ftsjd2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA to produce m7GpppNmp (cap1). Cap1 modification is linked to higher levels of translation By similarity.

Catalytic activity

S-adenosyl-L-methionine + m7G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA.

Subunit structure

Interacts with POLR2A (via C-terminus) By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the methyltransferase superfamily. RrmJ family.

Contains 1 G-patch domain.

Contains 1 WW domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 837837Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
PRO_0000251242

Regions

Domain86 – 13247G-patch
Domain751 – 78535WW
Region726 – 834109Interaction with POLR2A By similarity

Sites

Active site4031Proton acceptor By similarity
Binding site2801S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site3101S-adenosyl-L-methionine By similarity
Binding site3631S-adenosyl-L-methionine By similarity

Amino acid modifications

Modified residue271Phosphoserine By similarity
Modified residue301Phosphoserine By similarity
Modified residue1071N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5U2Z5 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 443BFE2882E687CA

FASTA83795,619
        10         20         30         40         50         60 
MKRRTDPECT APLKKQKRIG ELARHLSSTS DDEPLSSVSH AAKASTTSLS GSDSETEGKQ 

        70         80         90        100        110        120 
PCSDGFKDAF KADSLVEGTS SRYSMYNSVS QKLMAKMGFR EGEGLGKYSQ GRKDIVETSN 

       130        140        150        160        170        180 
QKGRRGLGLT LQGFDQELNV DWRDEPEPNA CEQVSWFPEC TTEIPDSREM SDWMVVGKRK 

       190        200        210        220        230        240 
MVIEDETEFC GEELLHSMLK CKSVFDILDG EEMRRARTRA NPYEMIRGVF FLNRAAMKMA 

       250        260        270        280        290        300 
NMDFVFDRMF TNPLDSSGKP LLKESDIDLL YFADVCAGPG GFSEYVLWRR KWHAKGFGMT 

       310        320        330        340        350        360 
LKGPNDFKLE DFYSASSELF EPYYGEGGVD GDGDITRPEN INAFRNFVLD NTDRKGVHFV 

       370        380        390        400        410        420 
MADGGFSVEG QENLQEILSK QLLLCQFLMA LSVVRTGGHF VCKTFDLFTP FSVGLIYLLY 

       430        440        450        460        470        480 
CCFERVCLFK PVTSRPANSE RYVVCKGLKV GIDDVREYLF SVNIQLNQLR NTDSDVNLVV 

       490        500        510        520        530        540 
PLSVIKGDHE FNDYMIRSNE SYCSLQIKAL AKIHAFVQDT TLSEPRQAEI RKECLQLWEI 

       550        560        570        580        590        600 
PDRARVAPSP SDPKFKFFEL IKDTDINIFS YKPTLLTAKT LEKIRPVLEY RCMVSGSEQK 

       610        620        630        640        650        660 
FLLGLGKSQI YTWDGRQSDR WVKLDLKTEL PRDTLLCVEI VHELKGEGKA QRKISAIHIL 

       670        680        690        700        710        720 
DVLVLNGSDV REQHFNQRIQ LAEKFVKAVS KPSRPDMNPI RVKEVYRLEE MEKIFVRLEM 

       730        740        750        760        770        780 
KLIKGSGGTP KLSYTGRDDR HFVPTGVYIV RTVTEPWTMA FSKGWKKKFF YNKKTGESFF 

       790        800        810        820        830 
TLPPESIAPF HTCYYTRLFW EWGDGFHMRD SQKPQDPDKL SKEDVLSFIQ SHNPLGP

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC085799 mRNA. Translation: AAH85799.1.
IPIIPI00559499.
RefSeqNP_001014053.1. NM_001014031.1.
UniGeneRn.17113.

3D structure databases

ProteinModelPortalQ5U2Z5.
ModBaseSearch...

PTM databases

PhosphoSiteQ5U2Z5.

Proteomic databases

PaxDbQ5U2Z5.
PRIDEQ5U2Z5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000640; ENSRNOP00000000640; ENSRNOG00000000532.
GeneID309656.
KEGGrno:309656.
UCSCRGD:1307801. rat.

Organism-specific databases

CTD23070.
RGD1307801. Ftsjd2.

Phylogenomic databases

eggNOGNOG319576.
GeneTreeENSGT00530000063742.
HOVERGENHBG057271.
KOK14589.

Gene expression databases

ArrayExpressQ5U2Z5.
GenevestigatorQ5U2Z5.
GermOnlineENSRNOG00000000532. Rattus norvegicus.

Family and domain databases

InterProIPR025816. Cap_mRNA_MeTrfase_1.
IPR000467. G_patch_dom.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR001202. WW_dom.
[Graphical view]
PfamPF01728. FtsJ. 1 hit.
PF01585. G-patch. 1 hit.
[Graphical view]
SMARTSM00443. G_patch. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
PROSITEPS50174. G_PATCH. 1 hit.
PS01159. WW_DOMAIN_1. False negative.
PS50020. WW_DOMAIN_2. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio661159.

Entry information

Entry nameMTR1_RAT
AccessionPrimary (citable) accession number: Q5U2Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 7, 2004
Last modified: May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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