Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LIM domain-containing protein ajuba

Gene

Ajuba

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Cell adhesion, Cell cycle, RNA-mediated gene silencing, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.

Names & Taxonomyi

Protein namesi
Recommended name:
LIM domain-containing protein ajuba
Gene namesi
Name:Ajuba
Synonyms:Jub
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi620407. Ajuba.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548LIM domain-containing protein ajubaPRO_0000312627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 891PhosphoserineBy similarity
Modified residuei129 – 1291PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei273 – 2731PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by LATS2 during mitosis. Phosphorylated by AURKA (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2Z2.
PRIDEiQ5U2Z2.

PTM databases

iPTMnetiQ5U2Z2.
PhosphoSiteiQ5U2Z2.

Expressioni

Tissue specificityi

Expressed in the brain, testis, kidney, heart, lung and liver.1 Publication

Gene expression databases

GenevisibleiQ5U2Z2. RN.

Interactioni

Subunit structurei

Interacts with GRB2 and PIP5K1B. Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3 (By similarity). Interacts with SLC1A2. Interacts with EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with VHL (By similarity). Interacts (via LIM domains) with SNAI1 (via SNAG domain), SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017753.

Structurei

3D structure databases

ProteinModelPortaliQ5U2Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini346 – 40762LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini411 – 47161LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini472 – 54069LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 345345PreLIMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi290 – 2989Nuclear localization signalSequence analysis

Domaini

LIM region interacts with CTNNA1. The preLIM region binds directly actin filaments (By similarity).By similarity
LIM-2 and LIM-3 domains mediate the interaction with the N-terminal region of AURKA. The association between LATS2 and AJUBA required the second LIM domain of AJUBA (By similarity).By similarity

Sequence similaritiesi

Belongs to the zyxin/ajuba family.Curated
Contains 3 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1701. Eukaryota.
ENOG410Y3GP. LUCA.
GeneTreeiENSGT00760000119039.
HOGENOMiHOG000113139.
HOVERGENiHBG095660.
InParanoidiQ5U2Z2.
KOiK16682.
OMAiRQPPTNY.
OrthoDBiEOG793B7F.
PhylomeDBiQ5U2Z2.
TreeFamiTF320310.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 3 hits.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERLGEKASR LLEKLRLSDS GSAKFGRRKG EASRSGSDGT PGAGKGRLSG
60 70 80 90 100
LGGPRKSGHR GANGGPGDEA LEPAREQGPL DAERNARGSF EAQRFEGSFP
110 120 130 140 150
GGPPPTRALP LPLSSPPDFR LETTAPALSP RSSFASSSAS DASKPSSPRG
160 170 180 190 200
SLLLDGAGAS GAGGSRPCSN RTSGISMGYD QRHGSPLPAG PCLFGLPLTT
210 220 230 240 250
APSGYSSGGV PSAYPELHAA LDRLCAHRPV GFGCQESRHS YPPALGSPGA
260 270 280 290 300
LTGAVVGTAG PLERRGTQPG RHSVTGYGDC AAGARYQDEL TALLRLTVAT
310 320 330 340 350
GGREAGARGE PLGIEPSGLE ESPGSFVPEA SRSRIREPEA REDYFGTCIK
360 370 380 390 400
CNKGIYGQSN ACQALDSLYH TQCFVCCSCG RTLRCKAFYS VNGSVYCEED
410 420 430 440 450
YLFSGFQEAA EKCCVCGHLI LEKILQAMGK SYHPGCFRCI VCNKCLDGVP
460 470 480 490 500
FTVDFSNQVY CVTDYHKNYA PKCAACGQPI LPSEGCEDIV RVISMDRDYH
510 520 530 540
FECYHCEDCR MQLSDEEGCC CFPLDGHLLC HGCHMQRLSA RQPPTNYI
Length:548
Mass (Da):58,081
Last modified:December 7, 2004 - v1
Checksum:iAC0C2D897DAF8907
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti343 – 3442DY → GD in CAC28536 (PubMed:11860269).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085802 mRNA. Translation: AAH85802.1.
AJ306292 mRNA. Translation: CAC28536.1.
RefSeqiNP_445955.1. NM_053503.1.
UniGeneiRn.144617.

Genome annotation databases

EnsembliENSRNOT00000017753; ENSRNOP00000017753; ENSRNOG00000012791.
GeneIDi85265.
KEGGirno:85265.
UCSCiRGD:620407. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085802 mRNA. Translation: AAH85802.1.
AJ306292 mRNA. Translation: CAC28536.1.
RefSeqiNP_445955.1. NM_053503.1.
UniGeneiRn.144617.

3D structure databases

ProteinModelPortaliQ5U2Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017753.

PTM databases

iPTMnetiQ5U2Z2.
PhosphoSiteiQ5U2Z2.

Proteomic databases

PaxDbiQ5U2Z2.
PRIDEiQ5U2Z2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017753; ENSRNOP00000017753; ENSRNOG00000012791.
GeneIDi85265.
KEGGirno:85265.
UCSCiRGD:620407. rat.

Organism-specific databases

CTDi84962.
RGDi620407. Ajuba.

Phylogenomic databases

eggNOGiKOG1701. Eukaryota.
ENOG410Y3GP. LUCA.
GeneTreeiENSGT00760000119039.
HOGENOMiHOG000113139.
HOVERGENiHBG095660.
InParanoidiQ5U2Z2.
KOiK16682.
OMAiRQPPTNY.
OrthoDBiEOG793B7F.
PhylomeDBiQ5U2Z2.
TreeFamiTF320310.

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.

Miscellaneous databases

PROiQ5U2Z2.

Gene expression databases

GenevisibleiQ5U2Z2. RN.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 3 hits.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba."
    Marie H., Billups D., Bedford F.K., Dumoulin A., Goyal R.K., Longmore G.D., Moss S.J., Attwell D.
    Mol. Cell. Neurosci. 19:152-164(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-548, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SLC1A2.

Entry informationi

Entry nameiAJUBA_RAT
AccessioniPrimary (citable) accession number: Q5U2Z2
Secondary accession number(s): Q99ND4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

'Ajuba' means 'curiosity' in Urdu, an Indian dialect.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.