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Protein

General transcription factor II-I

Gene

Gtf2i

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor II-I
Short name:
GTFII-I
Short name:
TFII-I
Gene namesi
Name:Gtf2i
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi727961. Gtf2i.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 979978General transcription factor II-IPRO_0000343749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei19 – 191PhosphoserineBy similarity
Cross-linki86 – 86Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki94 – 94Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei103 – 1031PhosphoserineBy similarity
Modified residuei130 – 1301N6-acetyllysine; alternateBy similarity
Cross-linki130 – 130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei210 – 2101PhosphoserineBy similarity
Modified residuei214 – 2141PhosphoserineBy similarity
Cross-linki221 – 221Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki221 – 221Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei248 – 2481Phosphotyrosine; by BTKBy similarity
Modified residuei298 – 2981Phosphotyrosine; by BTKBy similarity
Cross-linki306 – 306Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki324 – 324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei334 – 3341N6-acetyllysine; alternateBy similarity
Cross-linki334 – 334Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei379 – 3791Phosphotyrosine; by BTKBy similarity
Modified residuei393 – 3931Phosphoserine; by PKG/PRKG1By similarity
Modified residuei431 – 4311N6-acetyllysineBy similarity
Cross-linki437 – 437Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki469 – 469Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei484 – 4841Phosphotyrosine; by BTKBy similarity
Modified residuei498 – 4981PhosphoserineBy similarity
Modified residuei537 – 5371PhosphothreonineBy similarity
Modified residuei539 – 5391PhosphothreonineBy similarity
Cross-linki542 – 542Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki641 – 641Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki645 – 645Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei649 – 6491PhosphoserineBy similarity
Modified residuei655 – 6551PhosphoserineBy similarity
Modified residuei696 – 6961N6-acetyllysine; alternateBy similarity
Cross-linki696 – 696Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei703 – 7031PhosphoserineBy similarity
Modified residuei765 – 7651Phosphoserine; by PKG/PRKG1By similarity
Cross-linki797 – 797Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei804 – 8041PhosphoserineBy similarity
Cross-linki972 – 972Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki972 – 972Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Isoform 2 (identifier: Q5U2Y1-2)
Modified residuei228 – 2281PhosphoserineCombined sources

Post-translational modificationi

Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-379, and perhaps, on Tyr-484 contributes to BTK-mediated transcriptional activation (By similarity).By similarity
Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5U2Y1.
PRIDEiQ5U2Y1.

PTM databases

iPTMnetiQ5U2Y1.
PhosphoSiteiQ5U2Y1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001479.
ExpressionAtlasiQ5U2Y1. baseline and differential.
GenevisibleiQ5U2Y1. RN.

Interactioni

Subunit structurei

Homodimer (Potential). Interacts with SRF and PHOX1. Binds a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1). Associates with the PH domain of Bruton's tyrosine kinase (BTK) (By similarity). May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with BTK and ARID3A (By similarity). Interacts with isoform beta of PRKG1 (By similarity).By similarityCurated

Protein-protein interaction databases

BioGridi261874. 2 interactions.
STRINGi10116.ENSRNOP00000002020.

Structurei

3D structure databases

ProteinModelPortaliQ5U2Y1.
SMRiQ5U2Y1. Positions 104-197, 340-435, 447-537, 552-630, 712-804, 835-935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati103 – 19795GTF2I-like 1Add
BLAST
Repeati333 – 42795GTF2I-like 2Add
BLAST
Repeati438 – 53295GTF2I-like 3Add
BLAST
Repeati543 – 63795GTF2I-like 4Add
BLAST
Repeati705 – 79995GTF2I-like 5Add
BLAST
Repeati840 – 93495GTF2I-like 6Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi300 – 3078Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi82 – 854Poly-Glu
Compositional biasi300 – 31920Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the TFII-I family.PROSITE-ProRule annotation
Contains 6 GTF2I-like repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410Y9GU. LUCA.
GeneTreeiENSGT00530000063863.
HOGENOMiHOG000112832.
HOVERGENiHBG051856.
InParanoidiQ5U2Y1.
KOiK03121.
PhylomeDBiQ5U2Y1.

Family and domain databases

Gene3Di3.90.1460.10. 6 hits.
InterProiIPR004212. GTF2I.
IPR016659. TF_II-I.
[Graphical view]
PfamiPF02946. GTF2I. 6 hits.
[Graphical view]
PIRSFiPIRSF016441. TF_II-I. 1 hit.
SUPFAMiSSF117773. SSF117773. 6 hits.
PROSITEiPS51139. GTF2I. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5U2Y1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQVAMSALP AEDEESSESR MVVTFLMSAL ESMCKELAKS KAEVACIAVY
60 70 80 90 100
ETDVFVVGTE RGRAFVNTRK DFQKDFVKYC VEEEEKAAEM HKMKCTTQAN
110 120 130 140 150
RMSVDAVEIE TLRKTVEDYF CFCYGKALGK STVVPVPYEK MLRDQSAVAV
160 170 180 190 200
QGLPEGVAFK HPEHYDLATL KWILENKAGI SFIIKRPFLE PKKHLGGRVM
210 220 230 240 250
AADADRPMLS PGGSCGPIKV KTEPTEDSGI SLEMAAVTVK EESEDPDYYQ
260 270 280 290 300
YNIQGSHHSS EGNEGAEVEV PAEDSTQHVP SETSEDPEVE VTIEDDDYSP
310 320 330 340 350
PTKRPKSSEP PPPPPVPEPT NAGKRKVREF NFEKWNARIT DLRKQVEELF
360 370 380 390 400
ERKYAQAIKA KGPVTIPYPL FQSHVEDLYV EGLPEGIPFR RPSTYGIPRL
410 420 430 440 450
ERILLAKERI RFVIKKHELL NSTREDLQLD KPASGVKEEW YARITKLRKM
460 470 480 490 500
VDQLFCKKFA EALGSTEAKA VPYQKFEAYP NDLYVEGLPE NIPFRSPSWY
510 520 530 540 550
GIPRLEKIIQ VGNRIKFVIK RPELLTHSTT EVTQPRTNTP VKEDWNVRIT
560 570 580 590 600
KLRKQVEEIF NLKFAQALGL TEAVKVPYPV FESNPEFLYV EGLPEGIPFR
610 620 630 640 650
SPTWFGIPRL ERIVRGSNKI KFVVKKPELV VSYLPPGMAS KINTKALQSP
660 670 680 690 700
KRPRSPGSNS KVPEIEVTVE GPNNNSPQTS TVRTPTQTNG SNVPFKPRGR
710 720 730 740 750
EFSFEAWNAK ITDLKQKVEN LFNEKCGEAL GLKQAVKVPF ALFESFPEDF
760 770 780 790 800
YVEGLPEGVP FRRPSTFGIP RLEKILRNKA KIKFIIKKPE MFETAIKEST
810 820 830 840 850
SSKSPPRKTN SSPSVNTTAS GVEDLNIIQV TIPDDDNERL SKVEKARQLR
860 870 880 890 900
EQVNDLFSRK FGEAIGMGFP VKVPYRKITI NPGCVVVDGM PPGVSFKAPS
910 920 930 940 950
YLEISSMRRI LDSAEFIKFT VIRPFPGLVI NNQLVDQNES EGPVIQESAE
960 970
ASQLEVPATE EIKETDGSSQ IKQEPDPTW
Length:979
Mass (Da):110,215
Last modified:July 22, 2008 - v2
Checksum:i82D35A08122C210D
GO
Isoform 2 (identifier: Q5U2Y1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-294: Missing.

Show »
Length:913
Mass (Da):103,025
Checksum:iECC28C7546A7A5CD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei229 – 29466Missing in isoform 2. 1 PublicationVSP_034698Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF547390 mRNA. Translation: AAO32678.1.
AF547391 mRNA. Translation: AAO32679.1.
BC085815 mRNA. Translation: AAH85815.1.
RefSeqiNP_001001512.2. NM_001001512.2. [Q5U2Y1-2]
UniGeneiRn.27575.

Genome annotation databases

EnsembliENSRNOT00000002020; ENSRNOP00000002020; ENSRNOG00000001479. [Q5U2Y1-1]
ENSRNOT00000040356; ENSRNOP00000040835; ENSRNOG00000001479. [Q5U2Y1-2]
GeneIDi353256.
KEGGirno:353256.
UCSCiRGD:727961. rat. [Q5U2Y1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF547390 mRNA. Translation: AAO32678.1.
AF547391 mRNA. Translation: AAO32679.1.
BC085815 mRNA. Translation: AAH85815.1.
RefSeqiNP_001001512.2. NM_001001512.2. [Q5U2Y1-2]
UniGeneiRn.27575.

3D structure databases

ProteinModelPortaliQ5U2Y1.
SMRiQ5U2Y1. Positions 104-197, 340-435, 447-537, 552-630, 712-804, 835-935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi261874. 2 interactions.
STRINGi10116.ENSRNOP00000002020.

PTM databases

iPTMnetiQ5U2Y1.
PhosphoSiteiQ5U2Y1.

Proteomic databases

PaxDbiQ5U2Y1.
PRIDEiQ5U2Y1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002020; ENSRNOP00000002020; ENSRNOG00000001479. [Q5U2Y1-1]
ENSRNOT00000040356; ENSRNOP00000040835; ENSRNOG00000001479. [Q5U2Y1-2]
GeneIDi353256.
KEGGirno:353256.
UCSCiRGD:727961. rat. [Q5U2Y1-1]

Organism-specific databases

CTDi2969.
RGDi727961. Gtf2i.

Phylogenomic databases

eggNOGiENOG410Y9GU. LUCA.
GeneTreeiENSGT00530000063863.
HOGENOMiHOG000112832.
HOVERGENiHBG051856.
InParanoidiQ5U2Y1.
KOiK03121.
PhylomeDBiQ5U2Y1.

Miscellaneous databases

PROiQ5U2Y1.

Gene expression databases

BgeeiENSRNOG00000001479.
ExpressionAtlasiQ5U2Y1. baseline and differential.
GenevisibleiQ5U2Y1. RN.

Family and domain databases

Gene3Di3.90.1460.10. 6 hits.
InterProiIPR004212. GTF2I.
IPR016659. TF_II-I.
[Graphical view]
PfamiPF02946. GTF2I. 6 hits.
[Graphical view]
PIRSFiPIRSF016441. TF_II-I. 1 hit.
SUPFAMiSSF117773. SSF117773. 6 hits.
PROSITEiPS51139. GTF2I. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTF2I_RAT
AccessioniPrimary (citable) accession number: Q5U2Y1
Secondary accession number(s): Q80SX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.