Reviewed, UniProtKB/Swiss-Prot Q5U2X5 (ACK1_RAT)

Names and origin

Protein names

Recommended name:
    Activated CDC42 kinase 1
      Short name=ACK-1
    EC=2.7.10.2
Alternative name(s):
    Tyrosine kinase non-receptor protein 2

Gene names

Name:	Tnk2
Synonyms:	Ack1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1040 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development By similarity. UniProtKB Q07912
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. UniProtKB Q07912
Cofactor	Magnesium By similarity. UniProtKB Q07912
Enzyme regulation	The SH3 domain appears to play an autoinhibitory role By similarity. UniProtKB O54967
Subunit structure	Interacts with CDC42. Interacts with activated CSPG4 By similarity. Interacts with AR By similarity. Interacts with WWOX By similarity. Interacts with MERTK (activated); stimulates autophosphorylation By similarity. May interact (phosphorylated) with HSP90AB1; maintains kinase activity By similarity. UniProtKB Q07912
Subcellular location	Cell membrane By similarity UniProtKB O54967.
Post-translational modification	Autophosphorylation regulates kinase activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain. Contains 1 SH3 domain. Contains 1 UBA domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	SH3 domain
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1040	1040	Activated CDC42 kinase 1		PRO_0000312830
Regions
	Domain	126 – 385	260	Protein kinase
	Domain	373 – 448	76	SH3
	Domain	958 – 998	41	UBA
	Nucleotide binding	132 – 140	9	ATP By similarity UniProtKB P00517
	Compositional bias	577 – 935	359	Pro-rich
Sites
	Active site	252	1	Proton acceptor By similarity UniProtKB P00517
	Binding site	158	1	ATP By similarity UniProtKB O54967
Amino acid modifications
	Modified residue	284	1	Phosphotyrosine; by autocatalysis By similarity UniProtKB Q07912
	Modified residue	517	1	Phosphothreonine By similarity
	Modified residue	518	1	Phosphotyrosine By similarity UniProtKB Q07912
	Modified residue	728	1	Phosphoserine By similarity
	Modified residue	785	1	Phosphoserine By similarity
	Modified residue	827	1	Phosphotyrosine By similarity UniProtKB Q07912
	Modified residue	859	1	Phosphotyrosine By similarity UniProtKB Q07912
	Modified residue	860	1	Phosphotyrosine By similarity UniProtKB Q07912
	Modified residue	881	1	Phosphoserine By similarity
	Modified residue	925	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5U2X5-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 0624944919F821F6	FASTA	1,040	115,068
	10 20 30 40 50 60 MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NITRLSHFEY VKNEDLEKIG MGRPGQRRLW 70 80 90 100 110 120 EAVKRRKAMC KRKSWMSKVF SGKRQEAEFP SHHSQSTFRK PSPTPGGLAG EGTLQSLTCL 130 140 150 160 170 180 IGEKDLRLLE KLGDGSFGVV RRGEWDAPAG KTVSVAVKCL KPDVLSQPEA MDDFIREVNA 190 200 210 220 230 240 MHSLDHRNLI RLYGVVLTPP MKMVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM 250 260 270 280 290 300 GYLESKRFIH RDLAARNLLL ATRDLVKIGD FGLMRALPQN DGHYVMQEHR KVPFAWCAPE 310 320 330 340 350 360 SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL PRPEDCPQDI 370 380 390 400 410 420 YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE EPDKLHIQMN DVITVIEGRA 430 440 450 460 470 480 ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS AQDISQPLQN SFIHTGHGDS DPRHCWGFPD 490 500 510 520 530 540 RIDELYLGNP MDPPDLLSVE LSTSRPTQHL GRMKKPTYDP VSEDPDPLSS DFKRLGLRKP 550 560 570 580 590 600 ALPRGLWLAK PSARVPGTKA GRSSGGEVTL IDFGEEPVAP TPRPCAPSLA QLAMDACSLL 610 620 630 640 650 660 DKTPPQSPTR ALPRPLHPTP VVDWDARPLP PPPAYDDVAQ DEDDFEVCSI NSTLVSAGLP 670 680 690 700 710 720 TGPSQGETNY AFVPEQAQLP PALEDNLFLP PQGGGQPPSS AQTAEIFQAL QQECMRQLQV 730 740 750 760 770 780 PTGQLTPSPT PGGDDKPQVP PRVPIPPRPT RPRVGLSPAP LGEEEASRWP GPSSPPRVPP 790 800 810 820 830 840 REPLSPQGSR TPSPLVPPGS SPLPHRLSSS PGKTMPTTQS FASDPKYATP QVIQAPGPRA 850 860 870 880 890 900 GPCILPIVRD GRKVSSTHYY LLPERPPYLE RYQRFLREAQ SPEEPAALPV PPLLPPPSTP 910 920 930 940 950 960 APAAPTATVR PMPQAAPDPK ANFSTNNSNP GAQPPSLRAS ARLPQRGCPG DGQEAARPAD 970 980 990 1000 1010 1020 KVQMLQAMVH GVTTEECQAA LRSHSWSIQR AAQYLKVEQL FGLGLRPRVE CHKVLEMFDW 1030 1040 NLEQAGCHLL GSCGPAHHKR

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC085825 mRNA. Translation: AAH85825.1.
IPI	IPI00362119.
RefSeq	NP_001008337.1.
UniGene	Rn.98335
3D structure databases
HSSP	HSSP built from PDB template 1U54 based on UniProtKB Q07912.
SMR	Q5U2X5. Positions 117-388, 392-444, 447-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5U2X5.
PTM databases
PhosphoSite	Q5U2X5.
Genome annotation databases
Ensembl	ENSRNOT00000002411; ENSRNOP00000002411; ENSRNOG00000001769; Rattus norvegicus. [Genome view]
GeneID	303882.
KEGG	rno:303882.
UCSC	NM_001008336. rat.
Organism-specific databases
CTD	303882.
RGD	1346409. Tnk2.
Phylogenomic databases
eggNOG	roNOG11609.
HOVERGEN	Q5U2X5.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248.
Gene expression databases
Genevestigator	Q5U2X5.
Family and domain databases
InterPro	IPR015116. GTPase_binding. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001452. SH3_domain. IPR015779. Tyr_kinase_non-rcpt_Ack_N. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view]
PANTHER	PTHR23256:SF265. ACK_tyr_kin_N. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF09027. GTPase_binding. 1 hit. PF00627. UBA. 1 hit. [Graphical view]
SMART	SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50002. SH3. 1 hit. PS50030. UBA. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	652234.

Entry information

Entry name

ACK1_RAT

Accession

Primary (citable) accession number: Q5U2X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 7, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)