Reviewed,
UniProtKB/Swiss-Prot Q5U2X5 (ACK1_RAT)
Last modified
February 9, 2010.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Activated CDC42 kinase 1 Short name=ACK-1 EC=2.7.10.2 Alternative name(s): Tyrosine kinase non-receptor protein 2 | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1040 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development By similarity. UniProtKB Q07912 |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. UniProtKB Q07912 |
| Cofactor | Magnesium By similarity. UniProtKB Q07912 |
| Enzyme regulation | The SH3 domain appears to play an autoinhibitory role By similarity. UniProtKB O54967 |
| Subunit structure | Interacts with CDC42. Interacts with activated CSPG4 By similarity. Interacts with AR By similarity. Interacts with WWOX By similarity. Interacts with MERTK (activated); stimulates autophosphorylation By similarity. May interact (phosphorylated) with HSP90AB1; maintains kinase activity By similarity. UniProtKB Q07912 |
| Subcellular location | Cell membrane By similarity UniProtKB O54967. |
| Post-translational modification | Autophosphorylation regulates kinase activity By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain. Contains 1 SH3 domain. Contains 1 UBA domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | SH3 domain |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activityInferred from electronic annotation. Source: EC protein bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1040 | 1040 | Activated CDC42 kinase 1 | PRO_0000312830 | |||||
Regions | |||||||||
| Domain | 126 – 385 | 260 | Protein kinase | ||||||
| Domain | 373 – 448 | 76 | SH3 | ||||||
| Domain | 958 – 998 | 41 | UBA | ||||||
| Nucleotide binding | 132 – 140 | 9 | ATP By similarity UniProtKB P00517 | ||||||
| Compositional bias | 577 – 935 | 359 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 252 | 1 | Proton acceptor By similarity UniProtKB P00517 | ||||||
| Binding site | 158 | 1 | ATP By similarity UniProtKB O54967 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 284 | 1 | Phosphotyrosine; by autocatalysis By similarity UniProtKB Q07912 | ||||||
| Modified residue | 517 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 518 | 1 | Phosphotyrosine By similarity UniProtKB Q07912 | ||||||
| Modified residue | 728 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 785 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 827 | 1 | Phosphotyrosine By similarity UniProtKB Q07912 | ||||||
| Modified residue | 859 | 1 | Phosphotyrosine By similarity UniProtKB Q07912 | ||||||
| Modified residue | 860 | 1 | Phosphotyrosine By similarity UniProtKB Q07912 | ||||||
| Modified residue | 881 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 925 | 1 | Phosphothreonine By similarity | ||||||
Sequences
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References
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC085825 mRNA. Translation: AAH85825.1. |
| IPI | IPI00362119. |
| RefSeq | NP_001008337.1. |
| UniGene | Rn.98335 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1U54 based on UniProtKB Q07912. |
| SMR | Q5U2X5. Positions 117-388, 392-444, 447-489. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5U2X5. |
PTM databases | |
| PhosphoSite | Q5U2X5. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000002411; ENSRNOP00000002411; ENSRNOG00000001769; Rattus norvegicus. [Genome view] |
| GeneID | 303882. |
| KEGG | rno:303882. |
| UCSC | NM_001008336. rat. |
Organism-specific databases | |
| CTD | 303882. |
| RGD | 1346409. Tnk2. |
Phylogenomic databases | |
| eggNOG | roNOG11609. |
| HOVERGEN | Q5U2X5. |
Enzyme and pathway databases | |
| BRENDA | 2.7.10.2. 248. |
Gene expression databases | |
| Genevestigator | Q5U2X5. |
Family and domain databases | |
| InterPro | IPR015116. GTPase_binding. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001452. SH3_domain. IPR015779. Tyr_kinase_non-rcpt_Ack_N. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view] |
| PANTHER | PTHR23256:SF265. ACK_tyr_kin_N. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit. |
| Pfam | PF09027. GTPase_binding. 1 hit. PF00627. UBA. 1 hit. [Graphical view] |
| SMART | SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50002. SH3. 1 hit. PS50030. UBA. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 652234. |
Entry information
| Entry name | ACK1_RAT | ||||||||
| Accession | Primary (citable) accession number: Q5U2X5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


