Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sperm-associated antigen 1

Gene

Spag1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). Binds GTP and has GTPase activity (By similarity). Plays a role in fertilization.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi630 – 6378GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Fertilization

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 1
Alternative name(s):
HSD-3.8
Infertility-related sperm protein Spag-1
Gene namesi
Name:Spag1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1310702. Spag1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893Sperm-associated antigen 1PRO_0000106326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei351 – 3511PhosphoserineCombined sources
Modified residuei703 – 7031PhosphoserineCombined sources
Modified residuei739 – 7391PhosphoserineCombined sources
Modified residuei740 – 7401PhosphoserineCombined sources
Modified residuei758 – 7581PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2X2.
PRIDEiQ5U2X2.

PTM databases

iPTMnetiQ5U2X2.
PhosphoSiteiQ5U2X2.

Expressioni

Tissue specificityi

Testis and sperm.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013801.

Structurei

3D structure databases

ProteinModelPortaliQ5U2X2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati213 – 24634TPR 1Add
BLAST
Repeati247 – 27933TPR 2Add
BLAST
Repeati280 – 31334TPR 3Add
BLAST
Repeati429 – 46335TPR 4Add
BLAST
Repeati471 – 50434TPR 5Add
BLAST
Repeati506 – 53833TPR 6Add
BLAST
Repeati605 – 63834TPR 7Add
BLAST
Repeati639 – 67234TPR 8Add
BLAST

Sequence similaritiesi

Contains 8 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000293399.
HOVERGENiHBG079204.
InParanoidiQ5U2X2.
KOiK19870.
OMAiPLQAWHP.
OrthoDBiEOG75QR4W.
PhylomeDBiQ5U2X2.
TreeFamiTF106251.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKVKDHPP LWGFGTTKTF KIPIEHLDFK YIENCSDVKQ LEKILCVLRS
60 70 80 90 100
GEEGYYPELT EFCEKRLTGL APRSRALRKD KPAATASSFS AEEWEKIDSD
110 120 130 140 150
LKSWVSEIKR EENTRHFHDP EKHPGVEDPL PPVRGSNSCP RGGKETSSKS
160 170 180 190 200
KTAKKRIPRD YAEWDKFDVE KECSKIDEDY KEKTVINNKA HLSKIETKID
210 220 230 240 250
TAGLTEKEKN FLANREKGKG NEAFYSGDYE EAVMYYTRSL SALPTATAYN
260 270 280 290 300
NRAQAEIKLQ RWSSALEDCE KALELEPGNI KALLRRATTY KHQNKFLEAV
310 320 330 340 350
DDLRKVLQAE PDNDLAKKTL SEVERELKNS EPASELQTKG KRMVIEEVEN
360 370 380 390 400
SGDEGGKGDE DDHEDDGVDM AAMGNIQKKL MVRSQGGRRS RRARTPMPGA
410 420 430 440 450
EQQEGQPETG TASTSDNHDL EERRAADSPG DLKSRGNELF RGGQFAEAAV
460 470 480 490 500
QYSGAIAQLE PTGSENADEL SILYSNRAAC YLKEGNCRGC IQDCDRALEL
510 520 530 540 550
QPFAVKPLLR RAMAYETLEQ YRSAYVDYIT VLKIDCRIQL ASDSVNRITR
560 570 580 590 600
ILTELDGPKW RERLPPIPAV PASEPLRVWH PAAETPDQDP CPNSCTPTIT
610 620 630 640 650
DEKMFQALKE EGNQLVKDKN YKDAISKYNE CLKINSKACA IYTNRALCYL
660 670 680 690 700
KLGQFEEAKL DCDKALQIDS KNVKASYRLE LAQKGLENCR ERVADPSQVV
710 720 730 740 750
LLSPDSSEAA RHLDTKNDTA PPSRERERRR IEIQEVDDSS DEEPERPAEA
760 770 780 790 800
SAVEEGWSAE RAGKIEVCKP RNAYEFGQVL STISARKDEE ACAQLLVFTA
810 820 830 840 850
PQDLPVLLSN KLEGDMLLLI MQSLKSHLVA KDPSLVCKHL LYLSKAERFE
860 870 880 890
MMLALTSKDQ KEQMAQLFDD LSDAQADCLT AEDIQALRRQ YVL
Length:893
Mass (Da):100,615
Last modified:December 7, 2004 - v1
Checksum:iE95507EFF0B3A49F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085828 mRNA. Translation: AAH85828.1.
RefSeqiNP_001012116.1. NM_001012116.1.
UniGeneiRn.45841.

Genome annotation databases

EnsembliENSRNOT00000013801; ENSRNOP00000013801; ENSRNOG00000010078.
GeneIDi315033.
KEGGirno:315033.
UCSCiRGD:1310702. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085828 mRNA. Translation: AAH85828.1.
RefSeqiNP_001012116.1. NM_001012116.1.
UniGeneiRn.45841.

3D structure databases

ProteinModelPortaliQ5U2X2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013801.

PTM databases

iPTMnetiQ5U2X2.
PhosphoSiteiQ5U2X2.

Proteomic databases

PaxDbiQ5U2X2.
PRIDEiQ5U2X2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013801; ENSRNOP00000013801; ENSRNOG00000010078.
GeneIDi315033.
KEGGirno:315033.
UCSCiRGD:1310702. rat.

Organism-specific databases

CTDi6674.
RGDi1310702. Spag1.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000293399.
HOVERGENiHBG079204.
InParanoidiQ5U2X2.
KOiK19870.
OMAiPLQAWHP.
OrthoDBiEOG75QR4W.
PhylomeDBiQ5U2X2.
TreeFamiTF106251.

Miscellaneous databases

PROiQ5U2X2.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Expression and function of the HSD-3.8 gene encoding a testis-specific protein."
    Lin W., Zhou X.F., Zhang M.L., Li Y., Miao S.Y., Wang L.F., Zong S.D., Koide S.S.
    Mol. Hum. Reprod. 7:811-818(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351; SER-703; SER-739 AND SER-740, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPAG1_RAT
AccessioniPrimary (citable) accession number: Q5U2X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Antibodies against SPAG1 interfere with fertilization.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.