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Protein

F-box only protein 9

Gene

Fbxo9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 9
Gene namesi
Name:Fbxo9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1310374. Fbxo9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435F-box only protein 9PRO_0000119888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2X1.
PRIDEiQ5U2X1.

PTM databases

PhosphoSiteiQ5U2X1.

Expressioni

Gene expression databases

GenevisibleiQ5U2X1. RN.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Interacts with TTI1 and TELO2; when TTI1 and TELO2 are phosphorylated by CK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010961.

Structurei

3D structure databases

ProteinModelPortaliQ5U2X1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 11534TPRAdd
BLAST
Domaini173 – 22452F-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 TPR repeat.Curated

Keywords - Domaini

TPR repeat

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ5U2X1.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG7GXPBB.
PhylomeDBiQ5U2X1.
TreeFamiTF324797.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAEEDCHS DAVRVGDEGH ESPAERDLQA QLQMFRAQWM FELTPGVGSS
60 70 80 90 100
NVESRPCRAG RSSILKAAAD KGRQELAKEE KARELFLKAV EEEQNGALYE
110 120 130 140 150
AIKFYRRAMQ LVPDIEFKIT YTRSPDGDGV GSSYIEDNED ASKMADLLSY
160 170 180 190 200
FQQQLTFQES VLKLCQPELE TSQTHISVLP MEVLMYIFRW VVSSDLDLRS
210 220 230 240 250
LEQLSLVCRG FYICARDPEI WRLACLKVWG RSCMKLVPFS SWREMFLERP
260 270 280 290 300
RVRFDGVYIS KTTYIRQGEQ SLDGFYRAWH QVEYYRYIRF FPDGHVMMLT
310 320 330 340 350
TPEEPPSIVP RLRTRNTRTD AILLGHYRLS QDADNQTKVF AVITKKKEEK
360 370 380 390 400
PLDHKYRYFR RVPVQEADHN FHVGLQLCSS GHQRFNKLIW IHHSCHITYR
410 420 430
STGETAVSAF DIDKMYTPLF FARVRSYTAF SERPL
Length:435
Mass (Da):50,689
Last modified:December 7, 2004 - v1
Checksum:i737BE1C8C2C2EAD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085831 mRNA. Translation: AAH85831.1.
RefSeqiNP_001011998.1. NM_001011998.1.
UniGeneiRn.3335.

Genome annotation databases

EnsembliENSRNOT00000010961; ENSRNOP00000010961; ENSRNOG00000008214.
GeneIDi300849.
KEGGirno:300849.
UCSCiRGD:1310374. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085831 mRNA. Translation: AAH85831.1.
RefSeqiNP_001011998.1. NM_001011998.1.
UniGeneiRn.3335.

3D structure databases

ProteinModelPortaliQ5U2X1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010961.

PTM databases

PhosphoSiteiQ5U2X1.

Proteomic databases

PaxDbiQ5U2X1.
PRIDEiQ5U2X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010961; ENSRNOP00000010961; ENSRNOG00000008214.
GeneIDi300849.
KEGGirno:300849.
UCSCiRGD:1310374. rat.

Organism-specific databases

CTDi26268.
RGDi1310374. Fbxo9.

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ5U2X1.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG7GXPBB.
PhylomeDBiQ5U2X1.
TreeFamiTF324797.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi647636.
PROiQ5U2X1.

Gene expression databases

GenevisibleiQ5U2X1. RN.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFBX9_RAT
AccessioniPrimary (citable) accession number: Q5U2X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 7, 2004
Last modified: December 9, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.