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Protein

CDKN2A-interacting protein

Gene

Cdkn2aip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates DNA damage response and cell proliferation in a dose-dependent manner through a number of signaling pathways involved in cell proliferation, apoptosis and senescence.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CDKN2A-interacting protein
Alternative name(s):
Collaborator of ARF
Gene namesi
Name:Cdkn2aip
Synonyms:Carf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1305302. Cdkn2aip.

Subcellular locationi

  • Nucleusnucleoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 570569CDKN2A-interacting proteinPRO_0000324341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei124 – 1241PhosphoserineBy similarity
Modified residuei234 – 2341PhosphoserineBy similarity

Post-translational modificationi

May be ubiquitinated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5U2X0.
PRIDEiQ5U2X0.

PTM databases

iPTMnetiQ5U2X0.
PhosphoSiteiQ5U2X0.

Interactioni

Subunit structurei

Interacts with CDKN2A/p14ARF, p53/TP53 and MDM2. Interacts with CHEK2 and MAPK3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000029705.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 52776DRBMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 410287Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the CARF family.Curated
Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IE8W. Eukaryota.
ENOG4111PSZ. LUCA.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000111305.
HOVERGENiHBG101830.
InParanoidiQ5U2X0.
OMAiRCEGETD.
OrthoDBiEOG7HQNBG.
PhylomeDBiQ5U2X0.
TreeFamiTF333807.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR021859. XTBD.
[Graphical view]
PfamiPF11952. XTBD. 1 hit.
[Graphical view]
PROSITEiPS50137. DS_RBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5U2X0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQEVSEYLS QNPRVAAWVE TLRCEGETDK HWRHRREFLL RNAGDLVPAT
60 70 80 90 100
EETADAESGA RSRQLQQLVS FSMAWANHVF LGCRYPQKVM DKILSMAEGI
110 120 130 140 150
KVTDAPIHTT RDELVAKVKK RGISSSNEGV EEPSKKRSIE GKNNSSVERD
160 170 180 190 200
HGKKSAKTDR SAQQENSSGS KGSSTKSESS GTSARSNSGV SHQNSSTSEG
210 220 230 240 250
DRSVCSQSSS NSSQVTSAGS GKASEPEAPD KHGSASFVSS LLKSSLNSHV
260 270 280 290 300
TKSTDSRQHS GSPRKNALEG SSVSVSQSSS EIEVPLLGSS GSSEVELPLL
310 320 330 340 350
SCKSSSETAS SGLTTKASSE ANISSSVSKN SSSSGTSLLM PKSSSTNTSL
360 370 380 390 400
LTSQVAASLL ASKSSSQSSG SVASKSTSLG SMSQLASKSS SQSSTSQLPS
410 420 430 440 450
KSTSQSSESS VKFTCRKLTN EDIKQKQPFF NRLYKTVAWK LVAVGGFSPN
460 470 480 490 500
VNHGELLNAA IEALKATLDV FFVPLKELAD LPQNKSSQES IVCELRCKSV
510 520 530 540 550
YLGTGCGKSK ENAKAVASRE ALKLFLKKKV VVKICKRKYR GNEIEDLVLL
560 570
DEESRPVNLP PALKHPQELL
Length:570
Mass (Da):60,744
Last modified:December 7, 2004 - v1
Checksum:iE31CB97382220015
GO
Isoform 2 (identifier: Q5U2X0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-119: VK → KG
     120-570: Missing.

Note: No experimental confirmation available.
Show »
Length:119
Mass (Da):13,512
Checksum:i52C65DF8C4D0B027
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei118 – 1192VK → KG in isoform 2. 1 PublicationVSP_032223
Alternative sequencei120 – 570451Missing in isoform 2. 1 PublicationVSP_032224Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085832 mRNA. Translation: AAH85832.1.
BC098694 mRNA. Translation: AAH98694.1.
RefSeqiNP_001014022.1. NM_001014000.1. [Q5U2X0-1]
XP_006253209.1. XM_006253147.2. [Q5U2X0-2]
UniGeneiRn.16007.

Genome annotation databases

EnsembliENSRNOT00000030519; ENSRNOP00000029705; ENSRNOG00000022736. [Q5U2X0-1]
GeneIDi306455.
KEGGirno:306455.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085832 mRNA. Translation: AAH85832.1.
BC098694 mRNA. Translation: AAH98694.1.
RefSeqiNP_001014022.1. NM_001014000.1. [Q5U2X0-1]
XP_006253209.1. XM_006253147.2. [Q5U2X0-2]
UniGeneiRn.16007.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000029705.

PTM databases

iPTMnetiQ5U2X0.
PhosphoSiteiQ5U2X0.

Proteomic databases

PaxDbiQ5U2X0.
PRIDEiQ5U2X0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000030519; ENSRNOP00000029705; ENSRNOG00000022736. [Q5U2X0-1]
GeneIDi306455.
KEGGirno:306455.

Organism-specific databases

CTDi55602.
RGDi1305302. Cdkn2aip.

Phylogenomic databases

eggNOGiENOG410IE8W. Eukaryota.
ENOG4111PSZ. LUCA.
GeneTreeiENSGT00530000063234.
HOGENOMiHOG000111305.
HOVERGENiHBG101830.
InParanoidiQ5U2X0.
OMAiRCEGETD.
OrthoDBiEOG7HQNBG.
PhylomeDBiQ5U2X0.
TreeFamiTF333807.

Miscellaneous databases

PROiQ5U2X0.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR021859. XTBD.
[Graphical view]
PfamiPF11952. XTBD. 1 hit.
[Graphical view]
PROSITEiPS50137. DS_RBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis and Thymus.
  2. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCARF_RAT
AccessioniPrimary (citable) accession number: Q5U2X0
Secondary accession number(s): Q4KM89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.