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Protein

Dimethyladenosine transferase 2, mitochondrial

Gene

Tfb2m

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei124S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei150S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-163282 Mitochondrial transcription initiation

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 2, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 2
Mitochondrial transcription factor B2
Short name:
mtTFB2
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
Gene namesi
Name:Tfb2m
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1307091 Tfb2m

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 44MitochondrionSequence analysisAdd BLAST44
ChainiPRO_000027318145 – 397Dimethyladenosine transferase 2, mitochondrialAdd BLAST353

Proteomic databases

PaxDbiQ5U2T7
PRIDEiQ5U2T7

Expressioni

Gene expression databases

BgeeiENSRNOG00000002695
GenevisibleiQ5U2T7 RN

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003618

Structurei

3D structure databases

ProteinModelPortaliQ5U2T7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0820 Eukaryota
COG0030 LUCA
GeneTreeiENSGT00510000048533
HOGENOMiHOG000060174
HOVERGENiHBG094037
InParanoidiQ5U2T7
KOiK17653
OMAiKWVPGCG
OrthoDBiEOG091G0DOP
PhylomeDBiQ5U2T7
TreeFamiTF325100

Family and domain databases

InterProiView protein in InterPro
IPR001737 KsgA/Erm
IPR020598 rRNA_Ade_methylase_Trfase_N
IPR029063 SAM-dependent_MTases
IPR016861 TFB2M
PANTHERiPTHR11727 PTHR11727, 1 hit
PTHR11727:SF13 PTHR11727:SF13, 1 hit
PfamiView protein in Pfam
PF00398 RrnaAD, 1 hit
SMARTiView protein in SMART
SM00650 rADc, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51689 SAM_RNA_A_N6_MT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U2T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLAMRLPP RLALSVLAGR GPSCILGSGA ATRKDWQERN RRSFSDLYTQ
60 70 80 90 100
PLPDCDFEES SSWTHKSRSE PTRHIACKKS ARNLVRDLLE HQNPSHQLIL
110 120 130 140 150
ECNPGPGILT GALLKAGARV VAFESEKMFI PHLESLRKNA DGELQVVHCD
160 170 180 190 200
FFKIDPRYQE LVRPDVNSHT IFQNLGIKAV PWSAGVPIKV FGILPNKHER
210 220 230 240 250
RLLWKILFDL YSCESIYRYG RVELNMFISE KEFRKLIATP KRPDLYQVLG
260 270 280 290 300
VLWQVACEIK FLHMEPWSSF SVHAENGHLE KSKHSESLNL LKQNLYLVRM
310 320 330 340 350
TPRRTLFTEN LSPLNYDMFF HLVKHCFGKR NAPIIHHLRS LSTVDPINIL
360 370 380 390
RQIRKRPGDT AAKMYPHDFK RLFETIERSE DSVFKWIYDY CSDDSEL
Length:397
Mass (Da):46,021
Last modified:December 7, 2004 - v1
Checksum:i8F0E6B9940F3455E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085870 mRNA Translation: AAH85870.1
RefSeqiNP_001008294.1, NM_001008293.1
UniGeneiRn.96104

Genome annotation databases

EnsembliENSRNOT00000003618; ENSRNOP00000003618; ENSRNOG00000002695
GeneIDi289307
KEGGirno:289307

Similar proteinsi

Entry informationi

Entry nameiTFB2M_RAT
AccessioniPrimary (citable) accession number: Q5U2T7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 7, 2004
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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