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Protein

Dimethyladenosine transferase 2, mitochondrial

Gene

Tfb2m

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei124 – 1241S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei150 – 1501S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-163282. Mitochondrial transcription initiation.
R-RNO-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase 2, mitochondrial (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 2
Mitochondrial transcription factor B2
Short name:
mtTFB2
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
Gene namesi
Name:Tfb2m
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1307091. Tfb2m.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444MitochondrionSequence analysisAdd
BLAST
Chaini45 – 397353Dimethyladenosine transferase 2, mitochondrialPRO_0000273181Add
BLAST

Proteomic databases

PaxDbiQ5U2T7.
PRIDEiQ5U2T7.

Expressioni

Gene expression databases

GenevisibleiQ5U2T7. RN.

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003618.

Structurei

3D structure databases

ProteinModelPortaliQ5U2T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0820. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00510000048533.
HOGENOMiHOG000060174.
HOVERGENiHBG094037.
InParanoidiQ5U2T7.
KOiK17653.
OMAiVIHCDFF.
OrthoDBiEOG7RNK0M.
PhylomeDBiQ5U2T7.
TreeFamiTF325100.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
IPR016861. TFB2M.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 2 hits.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
PIRSFiPIRSF027833. MtTFB2. 1 hit.
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U2T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLAMRLPP RLALSVLAGR GPSCILGSGA ATRKDWQERN RRSFSDLYTQ
60 70 80 90 100
PLPDCDFEES SSWTHKSRSE PTRHIACKKS ARNLVRDLLE HQNPSHQLIL
110 120 130 140 150
ECNPGPGILT GALLKAGARV VAFESEKMFI PHLESLRKNA DGELQVVHCD
160 170 180 190 200
FFKIDPRYQE LVRPDVNSHT IFQNLGIKAV PWSAGVPIKV FGILPNKHER
210 220 230 240 250
RLLWKILFDL YSCESIYRYG RVELNMFISE KEFRKLIATP KRPDLYQVLG
260 270 280 290 300
VLWQVACEIK FLHMEPWSSF SVHAENGHLE KSKHSESLNL LKQNLYLVRM
310 320 330 340 350
TPRRTLFTEN LSPLNYDMFF HLVKHCFGKR NAPIIHHLRS LSTVDPINIL
360 370 380 390
RQIRKRPGDT AAKMYPHDFK RLFETIERSE DSVFKWIYDY CSDDSEL
Length:397
Mass (Da):46,021
Last modified:December 7, 2004 - v1
Checksum:i8F0E6B9940F3455E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085870 mRNA. Translation: AAH85870.1.
RefSeqiNP_001008294.1. NM_001008293.1.
UniGeneiRn.96104.

Genome annotation databases

EnsembliENSRNOT00000003618; ENSRNOP00000003618; ENSRNOG00000002695.
GeneIDi289307.
KEGGirno:289307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085870 mRNA. Translation: AAH85870.1.
RefSeqiNP_001008294.1. NM_001008293.1.
UniGeneiRn.96104.

3D structure databases

ProteinModelPortaliQ5U2T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003618.

Proteomic databases

PaxDbiQ5U2T7.
PRIDEiQ5U2T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003618; ENSRNOP00000003618; ENSRNOG00000002695.
GeneIDi289307.
KEGGirno:289307.

Organism-specific databases

CTDi64216.
RGDi1307091. Tfb2m.

Phylogenomic databases

eggNOGiKOG0820. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00510000048533.
HOGENOMiHOG000060174.
HOVERGENiHBG094037.
InParanoidiQ5U2T7.
KOiK17653.
OMAiVIHCDFF.
OrthoDBiEOG7RNK0M.
PhylomeDBiQ5U2T7.
TreeFamiTF325100.

Enzyme and pathway databases

ReactomeiR-RNO-163282. Mitochondrial transcription initiation.
R-RNO-2151201. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

PROiQ5U2T7.

Gene expression databases

GenevisibleiQ5U2T7. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR029063. SAM-dependent_MTases.
IPR016861. TFB2M.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 2 hits.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
PIRSFiPIRSF027833. MtTFB2. 1 hit.
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiTFB2M_RAT
AccessioniPrimary (citable) accession number: Q5U2T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.