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Protein

Zinc finger protein PLAG1

Gene

Plag1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein PLAG1
Alternative name(s):
Pleiomorphic adenoma gene 1 protein
Gene namesi
Name:Plag1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1305286. Plag1.

Subcellular locationi

  • Nucleus

  • Note: Strong nucleolar localization when sumoylation is inhibited.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951091 – 499Zinc finger protein PLAG1Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki263Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated with SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1 (By similarity).By similarity
Acetylated by lysine acetyltransferase EP300; which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ5U2T6.
PRIDEiQ5U2T6.

Expressioni

Tissue specificityi

Expressed in heart, spleen, lung, kidney, brain, testis and epididymis but not in salivary glands.

Gene expression databases

BgeeiENSRNOG00000008846.
GenevisibleiQ5U2T6. RN.

Interactioni

Subunit structurei

Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011721.

Structurei

3D structure databases

ProteinModelPortaliQ5U2T6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 84Interaction with KPNA2By similarityAdd BLAST83
Regioni41 – 242Decreased nuclear import with localization in the nucleus but also in the cytoplasmBy similarityAdd BLAST202
Regioni243 – 499Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interactionBy similarityAdd BLAST257
Regioni243 – 383Repression domain; contains 3 sumoylation motifs and massively decrease transcription activityBy similarityAdd BLAST141
Regioni384 – 499Massively activates transcriptionBy similarityAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 25Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi369 – 488Ser-richAdd BLAST120

Domaini

C2H2-type zinc fingers 3 interacts with DNA-binding site G-clusterinc fingers. C2H2-type zinc fingers 6 and 7 interact with DNA-binding site core sequence (By similarity).By similarity

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00390000013640.
HOGENOMiHOG000294191.
HOVERGENiHBG053608.
InParanoidiQ5U2T6.
KOiK19484.
OMAiIDMDAVH.
OrthoDBiEOG091G061O.
PhylomeDBiQ5U2T6.
TreeFamiTF332024.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR027765. PLAG1.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 2 hits.
PTHR10032:SF218. PTHR10032:SF218. 2 hits.
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2T6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVIPGDLS EVRDTQKAPS GKRKRGESKP RKNFPCQLCD KAFNSVEKLK
60 70 80 90 100
VHSFSHTGER PYKCTHQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM
110 120 130 140 150
FHRKDHLKNH LHTHDPNKET FKCEECGKSY NTKLGFKRHL ALHAATSGDL
160 170 180 190 200
TCKVCLQTFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC ERRFYTRKDV
210 220 230 240 250
RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF
260 270 280 290 300
LDPFTCNMSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS
310 320 330 340 350
SGSTHQMITT LPLGMTCPID MDTVHPSHHL AFKCPFSSTS YAISIPEKEQ
360 370 380 390 400
PLKGEIESYL MELQGGAPSS SQDSQASSSK LGLEPQSGSP DDGAGDLSLS
410 420 430 440 450
KSSISISDPL NTPALDFSQL FNFIPLNGPP YNPLSVGSLG MSYSQDEAHS
460 470 480 490
SVSQLPTQTQ DLQDPANTVG LGSLHSLSAA FTSSLSSSTT LPRFHQAFQ
Length:499
Mass (Da):55,640
Last modified:December 7, 2004 - v1
Checksum:i8EC2FA9F25A1CC8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085871 mRNA. Translation: AAH85871.1.
RefSeqiNP_001008317.1. NM_001008316.1.
XP_008761747.1. XM_008763525.2.
XP_008761748.1. XM_008763526.2.
XP_017448710.1. XM_017593221.1.
XP_017448711.1. XM_017593222.1.
XP_017448712.1. XM_017593223.1.
UniGeneiRn.234443.

Genome annotation databases

EnsembliENSRNOT00000011721; ENSRNOP00000011721; ENSRNOG00000008846.
GeneIDi297804.
KEGGirno:297804.
UCSCiRGD:1305286. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085871 mRNA. Translation: AAH85871.1.
RefSeqiNP_001008317.1. NM_001008316.1.
XP_008761747.1. XM_008763525.2.
XP_008761748.1. XM_008763526.2.
XP_017448710.1. XM_017593221.1.
XP_017448711.1. XM_017593222.1.
XP_017448712.1. XM_017593223.1.
UniGeneiRn.234443.

3D structure databases

ProteinModelPortaliQ5U2T6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011721.

Proteomic databases

PaxDbiQ5U2T6.
PRIDEiQ5U2T6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011721; ENSRNOP00000011721; ENSRNOG00000008846.
GeneIDi297804.
KEGGirno:297804.
UCSCiRGD:1305286. rat.

Organism-specific databases

CTDi5324.
RGDi1305286. Plag1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00390000013640.
HOGENOMiHOG000294191.
HOVERGENiHBG053608.
InParanoidiQ5U2T6.
KOiK19484.
OMAiIDMDAVH.
OrthoDBiEOG091G061O.
PhylomeDBiQ5U2T6.
TreeFamiTF332024.

Miscellaneous databases

PROiQ5U2T6.

Gene expression databases

BgeeiENSRNOG00000008846.
GenevisibleiQ5U2T6. RN.

Family and domain databases

Gene3Di3.30.160.60. 6 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR027765. PLAG1.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 2 hits.
PTHR10032:SF218. PTHR10032:SF218. 2 hits.
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLAG1_RAT
AccessioniPrimary (citable) accession number: Q5U2T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 7, 2004
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.