Q5U2R7 (MESD_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LDLR chaperone MESD

Alternative name(s):
Mesoderm development candidate 2
Mesoderm development protein

Gene names

Name:	Mesdc2
Synonyms:	Mesd

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction By similarity.
Subunit structure	Monomer By similarity. Interacts with LRP5; the interaction prevents LRP5 from forming aggregates and chaperones LRP6 to the plasma membrane. Interacts with LRP6; the interaction prevents LRP6 from forming aggregates and chaperones LRP6 to the plasma membrane By similarity.
Subcellular location	Endoplasmic reticulum By similarity.
Domain	The chaperone domain provides a folding template for proper folding of the beta-propeller (BP) domains of LRP5/6 By similarity. The escort domain ensures LRP5/6 safe-trafficking from the ER to the Golgi by preventing premature ligand-binding By similarity.
Sequence similarities	Belongs to the MESD family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Chaperone
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from sequence or structural similarity. Source: UniProtKB protein localization to cell surface Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 224	195	LDLR chaperone MESD		PRO_0000240320
Regions
	Region	1 – 155	155	Chaperone domain By similarity
	Region	156 – 195	40	Escort domain By similarity
	Motif	221 – 224	4	Prevents secretion from ER
Amino acid modifications
	Glycosylation	192	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5U2R7 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 88B146EBB72B4EB8	FASTA	224	25,216
	10 20 30 40 50 60 MAASSWLRAV LLFLCASDLL LLSPPEAYAT DTPGEAITPP RKKKDIRDYN DADMARLLEQ 70 80 90 100 110 120 WEKDDDIEEG DLPEHKRPSA PIDFSKLDPG KPESILKMTK KGKTLMMFVT ISGNPTEKET 130 140 150 160 170 180 EEITSLWQGS LFNANYDVQR FIVGSDRAIF MLRDGSYAWE IKDFLVNQDR CAEVTLEGQM 190 200 210 220 YPGKGGGSKE KNKTKPEKGK KKEGDPKPRA SKEDNRAGSR REDL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC085892 mRNA. Translation: AAH85892.1.
IPI	IPI00190375.
RefSeq	NP_001008346.1. NM_001008345.1.
UniGene	Rn.41045.
3D structure databases
ProteinModelPortal	Q5U2R7.
SMR	Q5U2R7. Positions 89-184.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4580521.
STRING	10116.ENSRNOP00000052447.
Proteomic databases
PaxDb	Q5U2R7.
PRIDE	Q5U2R7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000055583; ENSRNOP00000052447; ENSRNOG00000012366.
GeneID	308796.
KEGG	rno:308796.
Organism-specific databases
CTD	23184.
RGD	1310344. Mesdc2.
Phylogenomic databases
eggNOG	NOG286690.
GeneTree	ENSGT00390000000993.
HOGENOM	HOG000230558.
OrthoDB	EOG4QRH59.
Gene expression databases
Genevestigator	Q5U2R7.
GermOnline	ENSRNOG00000012366. Rattus norvegicus.
Family and domain databases
InterPro	IPR019330. Mesoderm_development_cand-2. [Graphical view]
Pfam	PF10185. Mesd. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	659572.

Entry information

Entry name

MESD_RAT

Accession

Primary (citable) accession number: Q5U2R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	December 7, 2004
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program