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Protein

Methionine adenosyltransferase 2 subunit beta

Gene

Mat2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (Mat1a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase (Mat1a), S-adenosylmethionine synthase isoform type-2 (Mat2a), S-adenosylmethionine synthase isoform type-1 (Mat1a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93NADP; via carbonyl oxygenBy similarity1
Binding sitei97NADPBy similarity1
Binding sitei159NADPBy similarity1
Binding sitei185NADP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 40NADPBy similarity4
Nucleotide bindingi60 – 62NADPBy similarity3
Nucleotide bindingi71 – 72NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-RNO-156581. Methylation.
SABIO-RKQ5U2R0.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine adenosyltransferase 2 subunit beta
Alternative name(s):
Methionine adenosyltransferase II beta
Short name:
MAT II beta
Gene namesi
Name:Mat2b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1593534. Mat2b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002875232 – 334Methionine adenosyltransferase 2 subunit betaAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei309PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2R0.
PRIDEiQ5U2R0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003177.
ExpressionAtlasiQ5U2R0. baseline and differential.
GenevisibleiQ5U2R0. RN.

Interactioni

Subunit structurei

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain. NADP binding increases the affinity for MAT2A.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004269.

Structurei

3D structure databases

ProteinModelPortaliQ5U2R0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 334Required for interaction with MAT2ABy similarityAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQ9F. Eukaryota.
COG1091. LUCA.
GeneTreeiENSGT00390000006721.
HOGENOMiHOG000227713.
HOVERGENiHBG105851.
InParanoidiQ5U2R0.
KOiK00789.
OMAiYKMDHYA.
OrthoDBiEOG091G0AS1.
PhylomeDBiQ5U2R0.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U2R0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGREKELSI HFVPGCCQLV EEEVNIPSRR VLITGATGLL GRAVYKEFQQ
60 70 80 90 100
SNWHAVGCGF RRARPKFEQV NLLDSEAVHH LIHDFQPHVI VHCAAERRPD
110 120 130 140 150
VVESQPDAAS QLNVGASGNL AKEAAAIGAF LIYISSDYVF DGTNPPYTEE
160 170 180 190 200
DIPSPLNLYG KTKLDGEKAV LENNLGAAVL RIPVLYGEVE KLEESAVTVM
210 220 230 240 250
FDKVQFSNKS ANMDHWQQRF PTHVKDVASV CRQLAEKRML DPSIKGTFHW
260 270 280 290 300
SGNEQMTKYE MACAIADAFN LPSSHLRPIT DSPVIGAQRP KNAQLDCSKL
310 320 330
ETLGIGQRTP FRIGIKESLW PFLIDKRWRQ TVFH
Length:334
Mass (Da):37,375
Last modified:December 7, 2004 - v1
Checksum:iFA65863830981734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085899 mRNA. Translation: AAH85899.1.
RefSeqiNP_001037747.1. NM_001044282.1.
UniGeneiRn.1752.

Genome annotation databases

EnsembliENSRNOT00000004269; ENSRNOP00000004269; ENSRNOG00000003177.
GeneIDi683630.
KEGGirno:683630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085899 mRNA. Translation: AAH85899.1.
RefSeqiNP_001037747.1. NM_001044282.1.
UniGeneiRn.1752.

3D structure databases

ProteinModelPortaliQ5U2R0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004269.

Proteomic databases

PaxDbiQ5U2R0.
PRIDEiQ5U2R0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004269; ENSRNOP00000004269; ENSRNOG00000003177.
GeneIDi683630.
KEGGirno:683630.

Organism-specific databases

CTDi27430.
RGDi1593534. Mat2b.

Phylogenomic databases

eggNOGiENOG410IQ9F. Eukaryota.
COG1091. LUCA.
GeneTreeiENSGT00390000006721.
HOGENOMiHOG000227713.
HOVERGENiHBG105851.
InParanoidiQ5U2R0.
KOiK00789.
OMAiYKMDHYA.
OrthoDBiEOG091G0AS1.
PhylomeDBiQ5U2R0.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
ReactomeiR-RNO-156581. Methylation.
SABIO-RKQ5U2R0.

Miscellaneous databases

PROiQ5U2R0.

Gene expression databases

BgeeiENSRNOG00000003177.
ExpressionAtlasiQ5U2R0. baseline and differential.
GenevisibleiQ5U2R0. RN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
IPR029903. RmlD-like-bd.
[Graphical view]
PANTHERiPTHR10491. PTHR10491. 1 hit.
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAT2B_RAT
AccessioniPrimary (citable) accession number: Q5U2R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.