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Q5U2N4 (MKNK2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MAP kinase-interacting serine/threonine-protein kinase 2

EC=2.7.11.1
Alternative name(s):
MAP kinase signal-integrating kinase 2
Short name=MAPK signal-integrating kinase 2
Short name=Mnk2
Gene names
Name:Mknk2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Enhances the formation of EIF4F complex in pachytene spermatocytes, thus promoting mRNA translation during spermatogenesis. Displays a high basal kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As2O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Inhibited by CGP57380 and staurosporine By similarity.

Subunit structure

Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2; this interaction is promoted when MAPK pathways are repressed but repressed upon ERK proteins activation. Also binds to dephosphorylated MAPK3/ERK1 and MAPK1/ER2K. Interaction with phosphorylated MAPK3/ERK1 and MAPK1/ER2K protects it from dephosphorylation and inactivation. Interacts with ESR2 and EIF4E in the nucleus By similarity.

Subcellular location

Cytoplasm By similarity. NucleusPML body By similarity.

Post-translational modification

Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As2O3 treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459MAP kinase-interacting serine/threonine-protein kinase 2
PRO_0000226965

Regions

Domain84 – 368285Protein kinase
Nucleotide binding90 – 989ATP By similarity
Region160 – 1623Staurosporine binding By similarity
Motif60 – 667Nuclear localization signal By similarity
Motif438 – 4425MAP kinase binding By similarity

Sites

Active site2051Proton acceptor By similarity
Metal binding2991Zinc By similarity
Metal binding3111Zinc By similarity
Metal binding3141Zinc By similarity
Binding site1131ATP By similarity
Binding site2091Staurosporine By similarity

Amino acid modifications

Modified residue741Phosphoserine By similarity
Modified residue2441Phosphothreonine By similarity
Modified residue2491Phosphothreonine By similarity
Modified residue3791Phosphothreonine By similarity
Modified residue4311Phosphoserine By similarity
Modified residue4341Phosphoserine By similarity
Modified residue4461Phosphoserine By similarity
Modified residue4501Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5U2N4 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 7D26909FB98AC34D

FASTA45951,574
        10         20         30         40         50         60 
MVQKRTAELQ GFHRSFKGQN PFELAFTLDP AQHGDSDFSP QCEARPDMPS SQPIDIPDAK 

        70         80         90        100        110        120 
KRGRKKKRCR ATDSFSGRFE DVYQLQEDVL GEGAHARVQT CVNLITNQEY AVKIIEKQLG 

       130        140        150        160        170        180 
HIRSRVFREV EMLYQCQGHR NVLELIEFFE EEDRFYLVFE KMRGGSILSH IHRRRHFNEL 

       190        200        210        220        230        240 
EASVVVQDVA SALDFLHNKG IAHRDLKPEN ILCEHPNQVS PVKICDFDLG SGIKLNGDCS 

       250        260        270        280        290        300 
PISTPELLTP CGSAEYMAPE VVEAFSEEAS IYDKRCDLWS LGVILYILLS GYPPFVGHCG 

       310        320        330        340        350        360 
SDCGWDRGEA CPACQNMLFE SIQEGKYEFP DKDWSHISFA AKDLISKLLV RDAKQRLSAA 

       370        380        390        400        410        420 
QVLQHPWVQG CAPENTLPTP LVLQRNSCAK DLTSFAAEAI AMNRQLAQCE EDAGQDQPVL 

       430        440        450 
IRATSRCLQL SPPSQSKLAQ RRQRASLSAT PVVLVGDRV 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC085941 mRNA. Translation: AAH85941.1.
RefSeqNP_001011985.1. NM_001011985.1.
XP_006240945.1. XM_006240883.1.
UniGeneRn.231923.

3D structure databases

ProteinModelPortalQ5U2N4.
SMRQ5U2N4. Positions 71-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000039672.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000041106; ENSRNOP00000039672; ENSRNOG00000029028.
GeneID299618.
KEGGrno:299618.
UCSCRGD:1305728. rat.

Organism-specific databases

CTD2872.
RGD1305728. Mknk2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110339.
HOGENOMHOG000231140.
HOVERGENHBG106949.
InParanoidQ5U2N4.
KOK04372.
OMAEPCHTCQ.
OrthoDBEOG7SV0VB.
PhylomeDBQ5U2N4.
TreeFamTF314050.

Gene expression databases

GenevestigatorQ5U2N4.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio645528.
PROQ5U2N4.

Entry information

Entry nameMKNK2_RAT
AccessionPrimary (citable) accession number: Q5U2N4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 7, 2004
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families