Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

MAP kinase-interacting serine/threonine-protein kinase 2

Gene

Mknk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Enhances the formation of EIF4F complex in pachytene spermatocytes, thus promoting mRNA translation during spermatogenesis. Displays a high basal kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As2O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by CGP57380 and staurosporine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113ATPPROSITE-ProRule annotation1
Active sitei205Proton acceptorPROSITE-ProRule annotation1
Binding sitei209StaurosporineBy similarity1
Metal bindingi299ZincBy similarity1
Metal bindingi311ZincBy similarity1
Metal bindingi314ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 98ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Translation regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-interacting serine/threonine-protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
MAP kinase signal-integrating kinase 2
Short name:
MAPK signal-integrating kinase 2
Short name:
Mnk2
Gene namesi
Name:Mknk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1305728. Mknk2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002269651 – 459MAP kinase-interacting serine/threonine-protein kinase 2Add BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74PhosphoserineBy similarity1
Modified residuei244PhosphothreonineBy similarity1
Modified residuei249PhosphothreonineBy similarity1
Modified residuei379PhosphothreonineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei450PhosphothreonineBy similarity1

Post-translational modificationi

Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As2O3 treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2N4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029028.
GenevisibleiQ5U2N4. RN.

Interactioni

Subunit structurei

Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2; this interaction is promoted when MAPK pathways are repressed but repressed upon ERK proteins activation. Also binds to dephosphorylated MAPK3/ERK1 and MAPK1/ER2K. Interaction with phosphorylated MAPK3/ERK1 and MAPK1/ER2K protects it from dephosphorylation and inactivation. Interacts with ESR2 and EIF4E in the nucleus (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039672.

Structurei

3D structure databases

ProteinModelPortaliQ5U2N4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 368Protein kinasePROSITE-ProRule annotationAdd BLAST285

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 162Staurosporine bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 66Nuclear localization signalBy similarity7
Motifi438 – 442MAP kinase bindingBy similarity5

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0607. Eukaryota.
ENOG410XQA9. LUCA.
GeneTreeiENSGT00830000128274.
HOGENOMiHOG000231140.
HOVERGENiHBG106949.
InParanoidiQ5U2N4.
KOiK04372.
OMAiVQKKTAE.
OrthoDBiEOG091G0G1X.
PhylomeDBiQ5U2N4.
TreeFamiTF314050.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQKRTAELQ GFHRSFKGQN PFELAFTLDP AQHGDSDFSP QCEARPDMPS
60 70 80 90 100
SQPIDIPDAK KRGRKKKRCR ATDSFSGRFE DVYQLQEDVL GEGAHARVQT
110 120 130 140 150
CVNLITNQEY AVKIIEKQLG HIRSRVFREV EMLYQCQGHR NVLELIEFFE
160 170 180 190 200
EEDRFYLVFE KMRGGSILSH IHRRRHFNEL EASVVVQDVA SALDFLHNKG
210 220 230 240 250
IAHRDLKPEN ILCEHPNQVS PVKICDFDLG SGIKLNGDCS PISTPELLTP
260 270 280 290 300
CGSAEYMAPE VVEAFSEEAS IYDKRCDLWS LGVILYILLS GYPPFVGHCG
310 320 330 340 350
SDCGWDRGEA CPACQNMLFE SIQEGKYEFP DKDWSHISFA AKDLISKLLV
360 370 380 390 400
RDAKQRLSAA QVLQHPWVQG CAPENTLPTP LVLQRNSCAK DLTSFAAEAI
410 420 430 440 450
AMNRQLAQCE EDAGQDQPVL IRATSRCLQL SPPSQSKLAQ RRQRASLSAT

PVVLVGDRV
Length:459
Mass (Da):51,574
Last modified:December 7, 2004 - v1
Checksum:i7D26909FB98AC34D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085941 mRNA. Translation: AAH85941.1.
RefSeqiNP_001011985.1. NM_001011985.1.
UniGeneiRn.231923.

Genome annotation databases

EnsembliENSRNOT00000041106; ENSRNOP00000039672; ENSRNOG00000029028.
GeneIDi299618.
KEGGirno:299618.
UCSCiRGD:1305728. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085941 mRNA. Translation: AAH85941.1.
RefSeqiNP_001011985.1. NM_001011985.1.
UniGeneiRn.231923.

3D structure databases

ProteinModelPortaliQ5U2N4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039672.

Proteomic databases

PaxDbiQ5U2N4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041106; ENSRNOP00000039672; ENSRNOG00000029028.
GeneIDi299618.
KEGGirno:299618.
UCSCiRGD:1305728. rat.

Organism-specific databases

CTDi2872.
RGDi1305728. Mknk2.

Phylogenomic databases

eggNOGiKOG0607. Eukaryota.
ENOG410XQA9. LUCA.
GeneTreeiENSGT00830000128274.
HOGENOMiHOG000231140.
HOVERGENiHBG106949.
InParanoidiQ5U2N4.
KOiK04372.
OMAiVQKKTAE.
OrthoDBiEOG091G0G1X.
PhylomeDBiQ5U2N4.
TreeFamiTF314050.

Miscellaneous databases

PROiQ5U2N4.

Gene expression databases

BgeeiENSRNOG00000029028.
GenevisibleiQ5U2N4. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMKNK2_RAT
AccessioniPrimary (citable) accession number: Q5U2N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.