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Protein

Membrane-associated phosphatidylinositol transfer protein 1

Gene

Pitpnm1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated phosphatidylinositol transfer protein 1
Alternative name(s):
Phosphatidylinositol transfer protein, membrane-associated 1
Short name:
PITPnm 1
Pyk2 N-terminal domain-interacting receptor 2
Short name:
NIR-2
Gene namesi
Name:Pitpnm1
Synonyms:Nir2, Pitpnm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1306710. Pitpnm1.

Subcellular locationi

  • Cytoplasm By similarity
  • Golgi apparatusGolgi stack membrane By similarity; Peripheral membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
  • Lipid droplet By similarity
  • Cleavage furrow By similarity
  • Midbody By similarity

  • Note: Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12421242Membrane-associated phosphatidylinositol transfer protein 1PRO_0000232740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei282 – 2821PhosphothreonineBy similarity
Modified residuei287 – 2871PhosphothreonineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei319 – 3191PhosphoserineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity
Modified residuei329 – 3291PhosphoserineBy similarity
Modified residuei342 – 3421PhosphoserineCombined sources
Modified residuei345 – 3451PhosphoserineCombined sources
Modified residuei346 – 3461PhosphoserineCombined sources
Modified residuei373 – 3731PhosphoserineCombined sources
Modified residuei382 – 3821Phosphoserine; by CDK1By similarity
Modified residuei593 – 5931PhosphoserineCombined sources
Modified residuei600 – 6001PhosphoserineCombined sources
Modified residuei621 – 6211PhosphoserineCombined sources
Modified residuei894 – 8941PhosphoserineBy similarity
Modified residuei1235 – 12351PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1 (By similarity).By similarity
Phosphorylated on threonine residues upon treatment with oleic acid.By similarity
Phosphorylated on tyrosine residues by PTK2B.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5U2N3.
PRIDEiQ5U2N3.

PTM databases

iPTMnetiQ5U2N3.
PhosphoSiteiQ5U2N3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018553.
GenevisibleiQ5U2N3. RN.

Interactioni

Subunit structurei

Interacts with PIK4CA and VAPB. Interacts with PTK2B via its C-terminus. Interacts with RHOA. Has higher affinity for the inactive, GDP-bound form of RHOA. The CDK1-phosphorylated form interacts with PLK1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025084.

Structurei

3D structure databases

ProteinModelPortaliQ5U2N3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini684 – 878195DDHDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi310 – 31910Poly-Ser

Sequence similaritiesi

Contains 1 DDHD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000294231.
HOVERGENiHBG052733.
InParanoidiQ5U2N3.
OMAiVKILERW.
OrthoDBiEOG091G0BMT.
PhylomeDBiQ5U2N3.
TreeFamiTF312967.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR023214. HAD-like_dom.
IPR031315. LNS2/PITP.
IPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02862. DDHD. 1 hit.
PF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.
SMARTiSM01127. DDHD. 1 hit.
SM00775. LNS2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKEYHILL PMSLDEYQVA QLYMIQKKSR EESSGEGSGV EILANRPYTD
60 70 80 90 100
GPGGNGQYTH KVYHVGSHIP GWFRALLPKA ALQVEEESWN AYPYTRTRYT
110 120 130 140 150
CPFVEKFSIE IETYYLPDGG QQPNVFNLSG AERRQRILDT IDIVRDAVAP
160 170 180 190 200
GEYKAEEDPR LYRSVKTGRG PLADDWARTA AQTGPLMCAY KLCKVEFRYW
210 220 230 240 250
GMQAKIEQFI HDVGLRRVML RAHRQAWCWQ DEWIELSMAD IRALEEETAR
260 270 280 290 300
MLAQRMAKCN TGSEGPEAQT PGKPSTETQP GTRTAGTPDG PEAPPGPDAS
310 320 330 340 350
PDASFGKQWS SSSRSSYSSQ HGGGVSPQSL SEWRMQNIAR DSENSSEEEF
360 370 380 390 400
FDAHEGFSDS DEVFPKEMTK WNSNDFIDAF ASPTEVEGVP DPAIMATKGI
410 420 430 440 450
EDEARAPRDS EGLDGTGDLG VEACSVHALF LILHSGSILD SGPGDTNSKQ
460 470 480 490 500
ADVQTLSTAF EAVTRVHFPE ALGHVALRLV PCPPICAAAY ALVSNLSPYS
510 520 530 540 550
HDGDSLSRSQ DHIPLAALPL LATSSSRYQG AVATVIARTN QAYAAFLRSS
560 570 580 590 600
EGTGFCGQVV LIGDGVGGIL GFDALCHSAS AGTGSRGSSR RGSMNNEMLS
610 620 630 640 650
PEVGPVRDPL ADGVEVLGRA SPEPSALPAQ RTSSDMANPD PDGSQNSLQV
660 670 680 690 700
APTVTSGEPR RASTASCPPA SSEAPDGPTN AARLDFKVSG FFLFGSPLGL
710 720 730 740 750
VLALRKTVMP ALEVAQMRPA CEQIYNLFHA ADPCASRLEP LLAPKFQAIA
760 770 780 790 800
PLAVPRYQKF PLGDGSSLLL ADTLQTHSSL FLEELEMMVP STPTSASGAF
810 820 830 840 850
WKGNELGNEP AAQPAAPSTT SEVVKILDRW WGNKRIDYSL YCPEALTAFP
860 870 880 890 900
TVTLPHLFHA SYWESADVVA FILRQVIEKE RPQLTECEEP SIYSPAFPRE
910 920 930 940 950
KWQRKRTQVK IRNVTSNHRA SDTVVCEGRP QVLNGRFMYG PLDVVTLTGE
960 970 980 990 1000
KVDVYVMTQP LSGKWIHFGT EVTNSSGRLT FPVPSERALG IGVYPVRMVV
1010 1020 1030 1040 1050
RGDHTYAECC LTVVSRGTEA VVFSIDGSFT ASVSIMGSDP KVRAGAVDVV
1060 1070 1080 1090 1100
RHWQDSGYLI VYVTGRPDMQ KHRVVAWLSQ HNFPHGVVSF CDGLTHDPLR
1110 1120 1130 1140 1150
QKAMFLQSLV QEVELNIVAG YGSPKDVAVY AALGLSPSQT YIVGRAVRKL
1160 1170 1180 1190 1200
QAQCQFLSDG YVAHLGQLEA GSHSHAPSGP PRAALAKSSY AVAAPVDFLR
1210 1220 1230 1240
KQSQLLRSRG PSQVDLEGPG TPPTTLARGK TRSISLKLDS EE
Length:1,242
Mass (Da):134,984
Last modified:December 7, 2004 - v1
Checksum:iAEC9040877E2979E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085945 mRNA. Translation: AAH85945.1.
RefSeqiNP_001008370.1. NM_001008369.1.
UniGeneiRn.154692.

Genome annotation databases

EnsembliENSRNOT00000025084; ENSRNOP00000025084; ENSRNOG00000018553.
GeneIDi361694.
KEGGirno:361694.
UCSCiRGD:1306710. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085945 mRNA. Translation: AAH85945.1.
RefSeqiNP_001008370.1. NM_001008369.1.
UniGeneiRn.154692.

3D structure databases

ProteinModelPortaliQ5U2N3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025084.

PTM databases

iPTMnetiQ5U2N3.
PhosphoSiteiQ5U2N3.

Proteomic databases

PaxDbiQ5U2N3.
PRIDEiQ5U2N3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025084; ENSRNOP00000025084; ENSRNOG00000018553.
GeneIDi361694.
KEGGirno:361694.
UCSCiRGD:1306710. rat.

Organism-specific databases

CTDi9600.
RGDi1306710. Pitpnm1.

Phylogenomic databases

eggNOGiKOG3668. Eukaryota.
COG5083. LUCA.
GeneTreeiENSGT00760000119216.
HOGENOMiHOG000294231.
HOVERGENiHBG052733.
InParanoidiQ5U2N3.
OMAiVKILERW.
OrthoDBiEOG091G0BMT.
PhylomeDBiQ5U2N3.
TreeFamiTF312967.

Miscellaneous databases

PROiQ5U2N3.

Gene expression databases

BgeeiENSRNOG00000018553.
GenevisibleiQ5U2N3. RN.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR023214. HAD-like_dom.
IPR031315. LNS2/PITP.
IPR001666. PI_transfer.
IPR023393. START-like_dom.
[Graphical view]
PANTHERiPTHR10658. PTHR10658. 1 hit.
PfamiPF02862. DDHD. 1 hit.
PF02121. IP_trans. 1 hit.
[Graphical view]
PRINTSiPR00391. PITRANSFER.
SMARTiSM01127. DDHD. 1 hit.
SM00775. LNS2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPITM1_RAT
AccessioniPrimary (citable) accession number: Q5U2N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: December 7, 2004
Last modified: September 7, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.