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Protein

DDB1- and CUL4-associated factor 8

Gene

Dcaf8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 8
Alternative name(s):
WD repeat-containing protein 42A
Gene namesi
Name:Dcaf8
Synonyms:Wdr42a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1308513. Dcaf8.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: It shuttles between the nucleus and the cytoplasm. Nuclear import is mediated by KPNA1 and KPNB1 under the regulation of nuclear GTPase RAN. Nuclear export to the cytoplasm is XPO1 dependent.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591DDB1- and CUL4-associated factor 8PRO_0000296960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei22 – 221PhosphoserineCombined sources
Modified residuei99 – 991PhosphoserineCombined sources
Modified residuei123 – 1231PhosphoserineCombined sources
Modified residuei124 – 1241PhosphoserineCombined sources
Modified residuei198 – 1981Omega-N-methylarginine; by PRMT1By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ5U2M6.
PRIDEiQ5U2M6.

PTM databases

iPTMnetiQ5U2M6.
PhosphoSiteiQ5U2M6.

Expressioni

Gene expression databases

ExpressionAtlasiQ5U2M6. baseline and differential.
GenevisibleiQ5U2M6. RN.

Interactioni

Subunit structurei

Interacts with DDB1, CUL4A and CUL4B. Interacts with KPNA1, KPNB1 and XPO1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008944.

Structurei

3D structure databases

ProteinModelPortaliQ5U2M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati185 – 22440WD 1Add
BLAST
Repeati228 – 26942WD 2Add
BLAST
Repeati275 – 31541WD 3Add
BLAST
Repeati323 – 36341WD 4Add
BLAST
Repeati379 – 41840WD 5Add
BLAST
Repeati426 – 46641WD 6Add
BLAST
Repeati470 – 50940WD 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat DCAF8 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1334. Eukaryota.
ENOG410XQZP. LUCA.
GeneTreeiENSGT00530000062951.
HOGENOMiHOG000243561.
HOVERGENiHBG053807.
InParanoidiQ5U2M6.
KOiK11804.
OMAiDENQVHD.
OrthoDBiEOG7GBFX0.
PhylomeDBiQ5U2M6.
TreeFamiTF326071.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U2M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKRPSTDG RRDLANGSLS SSPEEMSGAE EGRETSSGIE VEASDLSLSL
60 70 80 90 100
TGDDGGPNRT STESRGTDTE SSGEEKDSDS MEDTGHYSIN DENRVHGHSD
110 120 130 140 150
EEEEEEQPRH RVQRKRASRD QDSSDDERAL EDWVSSETTA LPRPRWQALP
160 170 180 190 200
ALRERELGSS ARFVYEACGA RVFVQRFRLQ HGLEGHTGCV NTLHFNQRGT
210 220 230 240 250
WLASGSDDLK VVVWDWVRRQ PVLDFESGHK SNVFQAKFLP NSGDSTLAMC
260 270 280 290 300
ARDGQVRVAE LSATQCCKNT KRVAQHKGAS HKLALEPDSP CTFLSAGEDA
310 320 330 340 350
VVFTIDLRQD RPASKLVVTK EKEKKVGLYT IYVNPANTHQ FAVGGRDQFV
360 370 380 390 400
RIYDQRKIDE NENNGVLKKF CPHHLVNSES KANITCLVYS HDGTELLASY
410 420 430 440 450
NDEDIYLFNS SHSDGAQYIK RYKGHRNNAT VKGVNFYGPK SEFVVSGSDC
460 470 480 490 500
GHIFLWEKSS CQIIQFMEGD KGGVVNCLEP HPHLPVLATS GLDHDVKIWA
510 520 530 540 550
PTAEASTELT GLKDVIKKNK RERDEDSLHH TDLFDSHMLW FLMHHLRQRR
560 570 580 590
HHRRWREPGV GATDADSDES PSSSDTSDEE EGPDRVQCMP S
Length:591
Mass (Da):66,156
Last modified:December 7, 2004 - v1
Checksum:i47492CD82D820E60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085957 mRNA. Translation: AAH85957.1.
RefSeqiNP_001014253.1. NM_001014231.1.
XP_006250359.1. XM_006250297.2.
XP_006250360.1. XM_006250298.2.
UniGeneiRn.203073.
Rn.7282.

Genome annotation databases

EnsembliENSRNOT00000008944; ENSRNOP00000008944; ENSRNOG00000006785.
GeneIDi364050.
KEGGirno:364050.
UCSCiRGD:1308513. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085957 mRNA. Translation: AAH85957.1.
RefSeqiNP_001014253.1. NM_001014231.1.
XP_006250359.1. XM_006250297.2.
XP_006250360.1. XM_006250298.2.
UniGeneiRn.203073.
Rn.7282.

3D structure databases

ProteinModelPortaliQ5U2M6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008944.

PTM databases

iPTMnetiQ5U2M6.
PhosphoSiteiQ5U2M6.

Proteomic databases

PaxDbiQ5U2M6.
PRIDEiQ5U2M6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008944; ENSRNOP00000008944; ENSRNOG00000006785.
GeneIDi364050.
KEGGirno:364050.
UCSCiRGD:1308513. rat.

Organism-specific databases

CTDi50717.
RGDi1308513. Dcaf8.

Phylogenomic databases

eggNOGiKOG1334. Eukaryota.
ENOG410XQZP. LUCA.
GeneTreeiENSGT00530000062951.
HOGENOMiHOG000243561.
HOVERGENiHBG053807.
InParanoidiQ5U2M6.
KOiK11804.
OMAiDENQVHD.
OrthoDBiEOG7GBFX0.
PhylomeDBiQ5U2M6.
TreeFamiTF326071.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ5U2M6.

Gene expression databases

ExpressionAtlasiQ5U2M6. baseline and differential.
GenevisibleiQ5U2M6. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-22; SER-99; SER-123 AND SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDCAF8_RAT
AccessioniPrimary (citable) accession number: Q5U2M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.