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Q5U258 (GCNT3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

EC=2.4.1.102
EC=2.4.1.150
Alternative name(s):
C2GnT-mucin type
Short name=C2GnT-M
Gene names
Name:gcnt3
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
PRO_0000288550

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3318Helical; Signal-anchor for type II membrane protein; Potential
Topological domain34 – 443410Lumenal Potential

Amino acid modifications

Glycosylation621N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Disulfide bond71 ↔ 228 By similarity
Disulfide bond162 ↔ 385 By similarity
Disulfide bond183 ↔ 210 By similarity
Disulfide bond394 ↔ 426 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5U258 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 403A28322EC561A7

FASTA44351,202
        10         20         30         40         50         60 
MSLMAHVRRK LCRGHLLRCT LPLTLLLFLT VGLKHLAIDC NLAHNNDIPM GSCMASYYTA 

        70         80         90        100        110        120 
LNLSAPGEKN CHRIIQGDTE EARRALLDNL MVKKRRLVAT DGDYINATSD CKFFRERRKY 

       130        140        150        160        170        180 
ITFSLSQVEQ DFPIAYSMVI HDNIEMFERL LRAVYTPHNI YCVHVDKKSP ESFQQAARAI 

       190        200        210        220        230        240 
TSCFDNVFVA SKLESVVYAS WRRVQADLNC MEDLLQSNVQ WRYLINTCGT DFPIKTNAEM 

       250        260        270        280        290        300 
VKALKSLNGH NSMESEIPPN YKKRRWEYHF ELKEDSNKIV QTNTRKKPSP LPVPVFSGNA 

       310        320        330        340        350        360 
YIVVTRNFVN SLFVNPTAKK FIMWAKDTYS PDEYMWATLH RFAEMPGHMP AHQKYDTSDI 

       370        380        390        400        410        420 
NAIARLVKWQ SLEGDMNKGA PYIPCTGTHR RQVCVYGTGD LNWMVQQHHF FANKFDPKVD 

       430        440 
DNAIQCLEEY LRYKTFHPTE NVA 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC086270 mRNA. Translation: AAH86270.1.
RefSeqNP_001088629.1. NM_001095160.1.
UniGeneXl.49113.

3D structure databases

ProteinModelPortalQ5U258.
SMRQ5U258. Positions 68-436.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID495681.
KEGGxla:495681.

Organism-specific databases

CTD9245.
XenbaseXB-GENE-5887147. gcnt3.

Phylogenomic databases

HOVERGENHBG051711.
KOK09662.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGCNT3_XENLA
AccessionPrimary (citable) accession number: Q5U258
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 7, 2004
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways