Reviewed, UniProtKB/Swiss-Prot Q5U258 (GCNT3_XENLA)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
    EC=2.4.1.102
    EC=2.4.1.150
Alternative name(s):
    C2GnT-mucin type
      Short name=C2GnT-M

Gene names

Name:

gcnt3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3		PRO_0000288550
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 33	18	Signal-anchor for type II membrane Potential
	Topological domain	34 – 443	410	Lumenal Potential
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Disulfide bond	71 ↔ 228		By similarity
	Disulfide bond	162 ↔ 385		By similarity
	Disulfide bond	183 ↔ 210		By similarity
	Disulfide bond	394 ↔ 426		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5U258-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 403A28322EC561A7	FASTA	443	51,202
	10 20 30 40 50 60 MSLMAHVRRK LCRGHLLRCT LPLTLLLFLT VGLKHLAIDC NLAHNNDIPM GSCMASYYTA 70 80 90 100 110 120 LNLSAPGEKN CHRIIQGDTE EARRALLDNL MVKKRRLVAT DGDYINATSD CKFFRERRKY 130 140 150 160 170 180 ITFSLSQVEQ DFPIAYSMVI HDNIEMFERL LRAVYTPHNI YCVHVDKKSP ESFQQAARAI 190 200 210 220 230 240 TSCFDNVFVA SKLESVVYAS WRRVQADLNC MEDLLQSNVQ WRYLINTCGT DFPIKTNAEM 250 260 270 280 290 300 VKALKSLNGH NSMESEIPPN YKKRRWEYHF ELKEDSNKIV QTNTRKKPSP LPVPVFSGNA 310 320 330 340 350 360 YIVVTRNFVN SLFVNPTAKK FIMWAKDTYS PDEYMWATLH RFAEMPGHMP AHQKYDTSDI 370 380 390 400 410 420 NAIARLVKWQ SLEGDMNKGA PYIPCTGTHR RQVCVYGTGD LNWMVQQHHF FANKFDPKVD 430 440 DNAIQCLEEY LRYKTFHPTE NVA

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC086270 mRNA. Translation: AAH86270.1.
RefSeq	NP_001088629.1.
UniGene	Xl.49113
3D structure databases
SMR	Q5U258. Positions 68-436.
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Genome annotation databases
GeneID	495681.
KEGG	xla:495681.
Organism-specific databases
CTD	495681.
Xenbase	XB-FEAT-5887145. gcnt3.
Phylogenomic databases
HOVERGEN	Q5U258.
Enzyme and pathway databases
BRENDA	2.4.1.102. 648. 2.4.1.150. 648.
Family and domain databases
InterPro	IPR021141. Core-2/I-Branching_enzyme. IPR003406. Glyco_trans_14. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT3_XENLA

Accession

Primary (citable) accession number: Q5U258

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	December 7, 2004
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project