Reviewed,
UniProtKB/Swiss-Prot Q5U258 (GCNT3_XENLA)
Last modified
February 9, 2010.
Version 32.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 EC=2.4.1.102 EC=2.4.1.150 Alternative name(s): C2GnT-mucin type Short name=C2GnT-M | ||
| Gene names |
| ||
| Organism | Xenopus laevis (African clawed frog) | ||
| Taxonomic identifier | 8355 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Protein attributes
| Sequence length | 443 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans By similarity. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R. UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 14 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 443 | 443 | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 | PRO_0000288550 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 15 | 15 | Cytoplasmic Potential | ||||||||
| Transmembrane | 16 – 33 | 18 | Signal-anchor for type II membrane Potential | ||||||||
| Topological domain | 34 – 443 | 410 | Lumenal Potential | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 62 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 106 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 71 ↔ 228 | By similarity | |||||||||
| Disulfide bond | 162 ↔ 385 | By similarity | |||||||||
| Disulfide bond | 183 ↔ 210 | By similarity | |||||||||
| Disulfide bond | 394 ↔ 426 | By similarity | |||||||||
Sequences
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References
| [1] | NIH - Xenopus Gene Collection (XGC) project Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC086270 mRNA. Translation: AAH86270.1. |
| RefSeq | NP_001088629.1. |
| UniGene | Xl.49113 |
3D structure databases | |
| SMR | Q5U258. Positions 68-436. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT14. Glycosyltransferase Family 14. |
Genome annotation databases | |
| GeneID | 495681. |
| KEGG | xla:495681. |
Organism-specific databases | |
| CTD | 495681. |
| Xenbase | XB-FEAT-5887145. gcnt3. |
Phylogenomic databases | |
| HOVERGEN | Q5U258. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.102. 648. 2.4.1.150. 648. |
Family and domain databases | |
| InterPro | IPR021141. Core-2/I-Branching_enzyme. IPR003406. Glyco_trans_14. [Graphical view] |
| PANTHER | PTHR19297. Glyco_trans_14. 1 hit. |
| Pfam | PF02485. Branch. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GCNT3_XENLA | ||||||||
| Accession | Primary (citable) accession number: Q5U258 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Xenopus annotation project | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


