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Q5U258

- GCNT3_XENLA

UniProt

Q5U258 - GCNT3_XENLA

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

gcnt3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Short name:
    C2GnT-M
    Gene namesi
    Name:gcnt3
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-5887147. gcnt3.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi71 ↔ 228By similarity
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi162 ↔ 385By similarity
    Disulfide bondi183 ↔ 210By similarity
    Disulfide bondi394 ↔ 426By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U258.
    SMRiQ5U258. Positions 68-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 443410LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3318Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG051711.
    KOiK09662.

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5U258-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLMAHVRRK LCRGHLLRCT LPLTLLLFLT VGLKHLAIDC NLAHNNDIPM    50
    GSCMASYYTA LNLSAPGEKN CHRIIQGDTE EARRALLDNL MVKKRRLVAT 100
    DGDYINATSD CKFFRERRKY ITFSLSQVEQ DFPIAYSMVI HDNIEMFERL 150
    LRAVYTPHNI YCVHVDKKSP ESFQQAARAI TSCFDNVFVA SKLESVVYAS 200
    WRRVQADLNC MEDLLQSNVQ WRYLINTCGT DFPIKTNAEM VKALKSLNGH 250
    NSMESEIPPN YKKRRWEYHF ELKEDSNKIV QTNTRKKPSP LPVPVFSGNA 300
    YIVVTRNFVN SLFVNPTAKK FIMWAKDTYS PDEYMWATLH RFAEMPGHMP 350
    AHQKYDTSDI NAIARLVKWQ SLEGDMNKGA PYIPCTGTHR RQVCVYGTGD 400
    LNWMVQQHHF FANKFDPKVD DNAIQCLEEY LRYKTFHPTE NVA 443
    Length:443
    Mass (Da):51,202
    Last modified:December 7, 2004 - v1
    Checksum:i403A28322EC561A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC086270 mRNA. Translation: AAH86270.1.
    RefSeqiNP_001088629.1. NM_001095160.1.
    UniGeneiXl.49113.

    Genome annotation databases

    GeneIDi495681.
    KEGGixla:495681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC086270 mRNA. Translation: AAH86270.1 .
    RefSeqi NP_001088629.1. NM_001095160.1.
    UniGenei Xl.49113.

    3D structure databases

    ProteinModelPortali Q5U258.
    SMRi Q5U258. Positions 68-436.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 495681.
    KEGGi xla:495681.

    Organism-specific databases

    CTDi 9245.
    Xenbasei XB-GENE-5887147. gcnt3.

    Phylogenomic databases

    HOVERGENi HBG051711.
    KOi K09662.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiGCNT3_XENLA
    AccessioniPrimary (citable) accession number: Q5U258
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3