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Protein

ATP-dependent RNA helicase DDX39A

Gene

Ddx39a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 95ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX39A (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 39
Nuclear RNA helicase, DECD variant of DEAD box family
Gene namesi
Name:Ddx39a
Synonyms:Ddx39, Ddxl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi619920. Ddx39a.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Can translocate to the cytoplasm in the presence of MX1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000550692 – 427ATP-dependent RNA helicase DDX39AAdd BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei35N6-acetyllysine; alternateBy similarity1
Cross-linki35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei37PhosphoserineBy similarity1
Modified residuei171PhosphothreonineBy similarity1
Modified residuei426PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5U216.
PRIDEiQ5U216.

PTM databases

iPTMnetiQ5U216.
PhosphoSitePlusiQ5U216.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004373.
GenevisibleiQ5U216. RN.

Interactioni

Subunit structurei

Binds ALYREF/THOC4 and DDX39B/BAT1. Interacts with SARNP (By similarity). Interacts with MX1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040666.

Structurei

3D structure databases

ProteinModelPortaliQ5U216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 248Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST174
Domaini260 – 421Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi44 – 72Q motifAdd BLAST29
Motifi195 – 198DECD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0329. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00830000128348.
HOVERGENiHBG107334.
InParanoidiQ5U216.
KOiK13182.
OMAiENFNDQH.
PhylomeDBiQ5U216.
TreeFamiTF300442.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQDVENEL LDYDEDEEPQ VPQESTPAPP KKDVKGSYVS IHSSGFRDFL
60 70 80 90 100
LKPELLRAIV DCGFEHPSEV QHECIPQAIL GMDVLCQAKS GMGKTAVFVL
110 120 130 140 150
ATLQQIEPIN GQVSVLVMCH TRELAFQISK EYERFSKYMP SVKVSVFFGG
160 170 180 190 200
LSIKKDEDVL KKNCPHVVVG TPGRILALVR SRSLNLRNVK HFVLDECDKM
210 220 230 240 250
LEQLDMRRDV QEIFRLTPHE KQCMMFSATL SKEIRPVCRK FMQDPMEVFV
260 270 280 290 300
DDETKLTLHG LQQYYVKLKD SEKNRKLFDL LDVLEFNQVV IFVKSVQRCM
310 320 330 340 350
ALAQLLVEQN FPAIAIHRGM AQEERLSRYQ QFKDFQRRIL VATNLFGRGM
360 370 380 390 400
DIERVNIVFN YDMPEDSDTY LHRVARAGRF GTKGLAVTFV SDENDAKILN
410 420
DVQDRFEVNV AELPEEIDIS TYIEQSR
Length:427
Mass (Da):49,109
Last modified:December 7, 2004 - v1
Checksum:iDB384747418FF290
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130K → T in AAC16391 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063447 mRNA. Translation: AAC16391.1.
BC086328 mRNA. Translation: AAH86328.1.
RefSeqiNP_446015.2. NM_053563.2.
XP_006255376.1. XM_006255314.3.
UniGeneiRn.161716.

Genome annotation databases

EnsembliENSRNOT00000045974; ENSRNOP00000040666; ENSRNOG00000004373.
ENSRNOT00000090312; ENSRNOP00000068949; ENSRNOG00000004373.
GeneIDi89827.
KEGGirno:89827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063447 mRNA. Translation: AAC16391.1.
BC086328 mRNA. Translation: AAH86328.1.
RefSeqiNP_446015.2. NM_053563.2.
XP_006255376.1. XM_006255314.3.
UniGeneiRn.161716.

3D structure databases

ProteinModelPortaliQ5U216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040666.

PTM databases

iPTMnetiQ5U216.
PhosphoSitePlusiQ5U216.

Proteomic databases

PaxDbiQ5U216.
PRIDEiQ5U216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000045974; ENSRNOP00000040666; ENSRNOG00000004373.
ENSRNOT00000090312; ENSRNOP00000068949; ENSRNOG00000004373.
GeneIDi89827.
KEGGirno:89827.

Organism-specific databases

CTDi10212.
RGDi619920. Ddx39a.

Phylogenomic databases

eggNOGiKOG0329. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00830000128348.
HOVERGENiHBG107334.
InParanoidiQ5U216.
KOiK13182.
OMAiENFNDQH.
PhylomeDBiQ5U216.
TreeFamiTF300442.

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiQ5U216.

Gene expression databases

BgeeiENSRNOG00000004373.
GenevisibleiQ5U216. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDX39A_RAT
AccessioniPrimary (citable) accession number: Q5U216
Secondary accession number(s): O70498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.