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Protein

Sorting nexin-3

Gene

Snx3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC). May act in part as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G. Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor Tfrc presuambly by delivering the tranferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex. Involved in regulation of neurite outgrowth in primary neurons.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei72Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei95Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei118Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-3
Gene namesi
Name:Snx3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1595151. Snx3.

Subcellular locationi

  • Early endosome By similarity
  • Cytoplasmic vesiclephagosome By similarity

  • Note: Colocalizes to clathrin-coated endosomal vesicles morphologically distinct from retromer-decorated non-branched endosomal tubule structures. Colocalizes with EEA1 on nascent phagosomes in dendritic cells but competes with EEA1 for binding to phagosomal membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002884752 – 162Sorting nexin-3Add BLAST161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei43Omega-N-methylarginineBy similarity1
Modified residuei72PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10 (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5U211.
PRIDEiQ5U211.

PTM databases

iPTMnetiQ5U211.
PhosphoSitePlusiQ5U211.

Interactioni

Subunit structurei

Interacts with VPS26A, VPS29 and VPS35; the interaction with VPS35 is direct. The association with the retromer CSC subcomplex subunits is proposed to represent a functional distinct retromer variant described as SNX3-retromer complex. Interacts with USP10 and SCNN1A. Interacts with TRFC. Interacts with SNX8; 2 molecules of SNX8 seems to associate with one molecule of SNX3. Interacts with PTPRU (By similarity).By similarity

Protein-protein interaction databases

BioGridi598983. 1 interactor.
STRINGi10116.ENSRNOP00000066574.

Structurei

3D structure databases

ProteinModelPortaliQ5U211.
SMRiQ5U211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 151PXPROSITE-ProRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 162Binds predominantly to PtdIns(P5) and weaker to PtdIns(P3) abd PtdIns(P4); involved in neurite outgrowth regulationBy similarityAdd BLAST16

Domaini

The PX domain mediates specific binding to phosphatidylinositol 3-phosphate (PtdIns(P3)).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2527. Eukaryota.
COG5391. LUCA.
HOVERGENiHBG055338.
InParanoidiQ5U211.
KOiK17918.
PhylomeDBiQ5U211.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5U211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETVADTRR LITKPQNLND AYGPPSNFLE IDVSNPQTVG VGRGRFTTYE
60 70 80 90 100
IRVKTNLPIF KLKESTVRRR YSDFEWLRSE LERESKVVVP PLPGKAFLRQ
110 120 130 140 150
LPFRGDDGIF DDNFIEERKQ GLEQFINKVA GHPLAQNERC LHMFLQDEII
160
DKSYTPSKIR HA
Length:162
Mass (Da):18,762
Last modified:December 7, 2004 - v1
Checksum:i983D422FCA6E07BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086341 mRNA. Translation: AAH86341.1.
BC107918 mRNA. Translation: AAI07919.1.
RefSeqiNP_001037748.1. NM_001044283.1.
UniGeneiRn.190405.
Rn.216668.
Rn.873.

Genome annotation databases

GeneIDi684097.
KEGGirno:684097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086341 mRNA. Translation: AAH86341.1.
BC107918 mRNA. Translation: AAI07919.1.
RefSeqiNP_001037748.1. NM_001044283.1.
UniGeneiRn.190405.
Rn.216668.
Rn.873.

3D structure databases

ProteinModelPortaliQ5U211.
SMRiQ5U211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi598983. 1 interactor.
STRINGi10116.ENSRNOP00000066574.

PTM databases

iPTMnetiQ5U211.
PhosphoSitePlusiQ5U211.

Proteomic databases

PaxDbiQ5U211.
PRIDEiQ5U211.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi684097.
KEGGirno:684097.

Organism-specific databases

CTDi8724.
RGDi1595151. Snx3.

Phylogenomic databases

eggNOGiKOG2527. Eukaryota.
COG5391. LUCA.
HOVERGENiHBG055338.
InParanoidiQ5U211.
KOiK17918.
PhylomeDBiQ5U211.

Miscellaneous databases

PROiQ5U211.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNX3_RAT
AccessioniPrimary (citable) accession number: Q5U211
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.