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Protein

Ribonuclease H2 subunit A

Gene

Rnaseh2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Divalent metal cationBy similarity
Metal bindingi35 – 351Divalent metal cationBy similarity
Metal bindingi142 – 1421Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
Name:Rnaseh2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1307248. Rnaseh2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Ribonuclease H2 subunit APRO_0000111712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei217 – 2171PhosphothreonineBy similarity
Modified residuei258 – 2581PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5U209.
PRIDEiQ5U209.

Expressioni

Gene expression databases

GenevisibleiQ5U209. RN.

Interactioni

Subunit structurei

The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049148.

Structurei

3D structure databases

ProteinModelPortaliQ5U209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

eggNOGiKOG2299. Eukaryota.
COG0164. LUCA.
GeneTreeiENSGT00390000010768.
HOGENOMiHOG000100290.
HOVERGENiHBG023585.
InParanoidiQ5U209.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG7KM5T4.
PhylomeDBiQ5U209.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5U209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSELERDN TGRCRLSSPV PAVCLKEPCV LGVDEAGRGP VLGPMVYAIC
60 70 80 90 100
YCPLSRLADL EALKVADSKT LTENERERLF AKMEEDGDFV GWALDILSPN
110 120 130 140 150
LISTSMLGRV KYNLNSMSHD TAAGLIQHAM DQNVKVTQVF VDTVGMPETY
160 170 180 190 200
QARLQQRFPG IEVTVKAKAD SLFPVVSAAS IIAKVARDQA VKNWQFVESL
210 220 230 240 250
QGLDSDYGSG YPNDPKTKAW LRKHVDPVFG FPQFVRFSWS TAQAILEKEA
260 270 280 290 300
ESVTWEDSAA EEDPEGPGRI TSYFSQGPQA CRPQVSHKYF QERGLETATS

L
Length:301
Mass (Da):33,286
Last modified:December 7, 2004 - v1
Checksum:i57796835026A5201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086345 mRNA. Translation: AAH86345.1.
BC086539 mRNA. Translation: AAH86539.1.
RefSeqiNP_001013252.1. NM_001013234.1.
XP_008770636.1. XM_008772414.1.
XP_008770637.1. XM_008772415.1.
XP_008770638.1. XM_008772416.1.
UniGeneiRn.3693.

Genome annotation databases

EnsembliENSRNOT00000046181; ENSRNOP00000049148; ENSRNOG00000003504.
ENSRNOT00000083159; ENSRNOP00000070694; ENSRNOG00000003504.
GeneIDi364974.
KEGGirno:364974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086345 mRNA. Translation: AAH86345.1.
BC086539 mRNA. Translation: AAH86539.1.
RefSeqiNP_001013252.1. NM_001013234.1.
XP_008770636.1. XM_008772414.1.
XP_008770637.1. XM_008772415.1.
XP_008770638.1. XM_008772416.1.
UniGeneiRn.3693.

3D structure databases

ProteinModelPortaliQ5U209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049148.

Proteomic databases

PaxDbiQ5U209.
PRIDEiQ5U209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000046181; ENSRNOP00000049148; ENSRNOG00000003504.
ENSRNOT00000083159; ENSRNOP00000070694; ENSRNOG00000003504.
GeneIDi364974.
KEGGirno:364974.

Organism-specific databases

CTDi10535.
RGDi1307248. Rnaseh2a.

Phylogenomic databases

eggNOGiKOG2299. Eukaryota.
COG0164. LUCA.
GeneTreeiENSGT00390000010768.
HOGENOMiHOG000100290.
HOVERGENiHBG023585.
InParanoidiQ5U209.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG7KM5T4.
PhylomeDBiQ5U209.

Miscellaneous databases

PROiQ5U209.

Gene expression databases

GenevisibleiQ5U209. RN.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Testis.

Entry informationi

Entry nameiRNH2A_RAT
AccessioniPrimary (citable) accession number: Q5U209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.