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Q5U203 (UBE2F_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NEDD8-conjugating enzyme UBE2F

EC=6.3.2.-
Alternative name(s):
NEDD8 carrier protein UBE2F
NEDD8 protein ligase UBE2F
Ubiquitin-conjugating enzyme E2 F
Gene names
Name:Ube2f
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5 By similarity.

Catalytic activity

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathway

Protein modification; protein neddylation.

Subunit structure

Interacts with UBA3 and RBX2 By similarity.

Post-translational modification

The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein neddylation

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NEDD8 ligase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5U203-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5U203-2)

The sequence of this isoform differs from the canonical sequence as follows:
     50-72: CTCKVHFPDPNKLHCFQLTVSPD → Y
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185NEDD8-conjugating enzyme UBE2F
PRO_0000374072

Sites

Active site1161Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence50 – 7223CTCKV…TVSPD → Y in isoform 2.
VSP_037303

Experimental info

Sequence conflict1811K → Q in DY311915. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 6F1DF65197DBF7A1

FASTA18521,080
        10         20         30         40         50         60 
MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPC TCKVHFPDPN 

        70         80         90        100        110        120 
KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL 

       130        140        150        160        170        180 
REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKE DFRDKVDEYI 


KRYAR 

« Hide

Isoform 2 [UniParc].

Checksum: 85F172287CCF8D5A
Show »

FASTA16318,631

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DY311915 mRNA. No translation available.
BC086355 mRNA. Translation: AAH86355.1.
RefSeqNP_001008382.1. NM_001008381.1.
XP_006245484.1. XM_006245422.1.
XP_006245485.1. XM_006245423.1.
XP_006245486.1. XM_006245424.1.
XP_006245487.1. XM_006245425.1.
XP_006245488.1. XM_006245426.1.
XP_006245489.1. XM_006245427.1.
UniGeneRn.62010.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000027050.

Proteomic databases

PRIDEQ5U203.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027050; ENSRNOP00000027050; ENSRNOG00000019953. [Q5U203-1]
ENSRNOT00000042082; ENSRNOP00000040354; ENSRNOG00000019953. [Q5U203-2]
GeneID363284.
KEGGrno:363284.
UCSCRGD:1307608. rat. [Q5U203-1]

Organism-specific databases

CTD140739.
RGD1307608. Ube2f.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00630000089859.
HOGENOMHOG000233456.
KOK10687.
OMAWHPNISE.
OrthoDBEOG7X9G8T.
PhylomeDBQ5U203.
TreeFamTF101125.

Enzyme and pathway databases

UniPathwayUPA00885.

Gene expression databases

GenevestigatorQ5U203.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio683120.
PROQ5U203.

Entry information

Entry nameUBE2F_RAT
AccessionPrimary (citable) accession number: Q5U203
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways