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Q5U203

- UBE2F_RAT

UniProt

Q5U203 - UBE2F_RAT

Protein

NEDD8-conjugating enzyme UBE2F

Gene

Ube2f

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5 By similarity.By similarity

    Catalytic activityi

    ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei116 – 1161Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NEDD8 ligase activity Source: UniProtKB

    GO - Biological processi

    1. protein neddylation Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00885.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NEDD8-conjugating enzyme UBE2F (EC:6.3.2.-)
    Alternative name(s):
    NEDD8 carrier protein UBE2F
    NEDD8 protein ligase UBE2F
    Ubiquitin-conjugating enzyme E2 F
    Gene namesi
    Name:Ube2f
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi1307608. Ube2f.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 185185NEDD8-conjugating enzyme UBE2FPRO_0000374072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ5U203.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5U203.

    Interactioni

    Subunit structurei

    Interacts with UBA3 and RBX2.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000027050.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5U203.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00630000089859.
    HOGENOMiHOG000233456.
    KOiK10687.
    OMAiWHPNISE.
    OrthoDBiEOG7X9G8T.
    PhylomeDBiQ5U203.
    TreeFamiTF101125.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5U203-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPC    50
    TCKVHFPDPN KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC 100
    LTKIWHPNIT ETGEICLSLL REHSIDGTGW APTRTLKDVV WGLNSLFTDL 150
    LNFDDPLNIE AAEHHLRDKE DFRDKVDEYI KRYAR 185
    Length:185
    Mass (Da):21,080
    Last modified:May 5, 2009 - v2
    Checksum:i6F1DF65197DBF7A1
    GO
    Isoform 2 (identifier: Q5U203-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         50-72: CTCKVHFPDPNKLHCFQLTVSPD → Y

    Note: No experimental confirmation available.

    Show »
    Length:163
    Mass (Da):18,631
    Checksum:i85F172287CCF8D5A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811K → Q in DY311915. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei50 – 7223CTCKV…TVSPD → Y in isoform 2. 1 PublicationVSP_037303Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DY311915 mRNA. No translation available.
    BC086355 mRNA. Translation: AAH86355.1.
    RefSeqiNP_001008382.1. NM_001008381.1. [Q5U203-2]
    XP_006245484.1. XM_006245422.1. [Q5U203-1]
    XP_006245485.1. XM_006245423.1. [Q5U203-1]
    XP_006245486.1. XM_006245424.1. [Q5U203-1]
    XP_006245487.1. XM_006245425.1. [Q5U203-1]
    XP_006245488.1. XM_006245426.1. [Q5U203-1]
    XP_006245489.1. XM_006245427.1. [Q5U203-1]
    UniGeneiRn.62010.

    Genome annotation databases

    EnsembliENSRNOT00000027050; ENSRNOP00000027050; ENSRNOG00000019953. [Q5U203-1]
    ENSRNOT00000042082; ENSRNOP00000040354; ENSRNOG00000019953. [Q5U203-2]
    GeneIDi363284.
    KEGGirno:363284.
    UCSCiRGD:1307608. rat. [Q5U203-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DY311915 mRNA. No translation available.
    BC086355 mRNA. Translation: AAH86355.1 .
    RefSeqi NP_001008382.1. NM_001008381.1. [Q5U203-2 ]
    XP_006245484.1. XM_006245422.1. [Q5U203-1 ]
    XP_006245485.1. XM_006245423.1. [Q5U203-1 ]
    XP_006245486.1. XM_006245424.1. [Q5U203-1 ]
    XP_006245487.1. XM_006245425.1. [Q5U203-1 ]
    XP_006245488.1. XM_006245426.1. [Q5U203-1 ]
    XP_006245489.1. XM_006245427.1. [Q5U203-1 ]
    UniGenei Rn.62010.

    3D structure databases

    ProteinModelPortali Q5U203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000027050.

    Proteomic databases

    PRIDEi Q5U203.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027050 ; ENSRNOP00000027050 ; ENSRNOG00000019953 . [Q5U203-1 ]
    ENSRNOT00000042082 ; ENSRNOP00000040354 ; ENSRNOG00000019953 . [Q5U203-2 ]
    GeneIDi 363284.
    KEGGi rno:363284.
    UCSCi RGD:1307608. rat. [Q5U203-1 ]

    Organism-specific databases

    CTDi 140739.
    RGDi 1307608. Ube2f.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00630000089859.
    HOGENOMi HOG000233456.
    KOi K10687.
    OMAi WHPNISE.
    OrthoDBi EOG7X9G8T.
    PhylomeDBi Q5U203.
    TreeFami TF101125.

    Enzyme and pathway databases

    UniPathwayi UPA00885 .

    Miscellaneous databases

    NextBioi 683120.
    PROi Q5U203.

    Gene expression databases

    Genevestigatori Q5U203.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Ovary.

    Entry informationi

    Entry nameiUBE2F_RAT
    AccessioniPrimary (citable) accession number: Q5U203
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3