Q5U203 (UBE2F_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD8-conjugating enzyme UBE2F
EC=6.3.2.-

Alternative name(s):
NEDD8 carrier protein UBE2F
NEDD8 protein ligase UBE2F
Ubiquitin-conjugating enzyme E2 F

Gene names

Name:

Ube2f

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5 By similarity.
Catalytic activity	ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.
Pathway	Protein modification; protein neddylation.
Subunit structure	Interacts with UBA3 and RBX2 By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein neddylation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NEDD8 ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5U203-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5U203-2)

The sequence of this isoform differs from the canonical sequence as follows:
     50-72: CTCKVHFPDPNKLHCFQLTVSPD → Y

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	NEDD8-conjugating enzyme UBE2F		PRO_0000374072
Sites
	Active site	116	1	Glycyl thioester intermediate By similarity
Natural variations
	Alternative sequence	50 – 72	23	CTCKV…TVSPD → Y in isoform 2.		VSP_037303
Experimental info
	Sequence conflict	181	1	K → Q in DY311915. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 6F1DF65197DBF7A1	FASTA	185	21,080
	10 20 30 40 50 60 MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPC TCKVHFPDPN 70 80 90 100 110 120 KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL 130 140 150 160 170 180 REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKE DFRDKVDEYI KRYAR
	Isoform 2 [UniParc]. Checksum: 85F172287CCF8D5A	FASTA	163	18,631
	10 20 30 40 50 60 MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPY EGYYQGGKFQ 70 80 90 100 110 120 FETEVPDAYN MVPPKVKCLT KIWHPNITET GEICLSLLRE HSIDGTGWAP TRTLKDVVWG 130 140 150 160 LNSLFTDLLN FDDPLNIEAA EHHLRDKEDF RDKVDEYIKR YAR

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DY311915 mRNA. No translation available. BC086355 mRNA. Translation: AAH86355.1.
IPI	IPI00367134. IPI00562665.
RefSeq	NP_001008382.1. NM_001008381.1.
UniGene	Rn.62010.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027050.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027050; ENSRNOP00000027050; ENSRNOG00000019953. ENSRNOT00000042082; ENSRNOP00000040354; ENSRNOG00000019953.
GeneID	363284.
KEGG	rno:363284.
UCSC	RGD:1307608. rat.
Organism-specific databases
CTD	140739.
RGD	1307608. Ube2f.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00630000089859.
HOGENOM	HOG000233456.
KO	K10687.
OMA	KFQFEIE.
OrthoDB	EOG4J1195.
Enzyme and pathway databases
UniPathway	UPA00885.
Gene expression databases
Genevestigator	Q5U203.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	683120.

Entry information

Entry name

UBE2F_RAT

Accession

Primary (citable) accession number: Q5U203

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program