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Protein

NEDD8-conjugating enzyme UBE2F

Gene

Ube2f

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5 (By similarity).By similarity

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. NEDD8 transferase activity Source: UniProtKB
  4. ubiquitin-like protein transferase activity Source: GO_Central

GO - Biological processi

  1. protein neddylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme UBE2F (EC:6.3.2.-)
Alternative name(s):
NEDD8 carrier protein UBE2F
NEDD8 protein ligase UBE2F
Ubiquitin-conjugating enzyme E2 F
Gene namesi
Name:Ube2f
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1307608. Ube2f.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185NEDD8-conjugating enzyme UBE2FPRO_0000374072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ5U203.

Expressioni

Gene expression databases

GenevestigatoriQ5U203.

Interactioni

Subunit structurei

Interacts with UBA3 and RBX2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027050.

Structurei

3D structure databases

ProteinModelPortaliQ5U203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00630000089859.
HOGENOMiHOG000233456.
InParanoidiQ5U203.
KOiK10687.
OMAiWHPNISE.
OrthoDBiEOG7X9G8T.
PhylomeDBiQ5U203.
TreeFamiTF101125.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5U203-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTLASKLKR DDGLKGSRAS ASTSDSTRRV SVRDKLLVKE VAELEANLPC
60 70 80 90 100
TCKVHFPDPN KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC
110 120 130 140 150
LTKIWHPNIT ETGEICLSLL REHSIDGTGW APTRTLKDVV WGLNSLFTDL
160 170 180
LNFDDPLNIE AAEHHLRDKE DFRDKVDEYI KRYAR
Length:185
Mass (Da):21,080
Last modified:May 5, 2009 - v2
Checksum:i6F1DF65197DBF7A1
GO
Isoform 2 (identifier: Q5U203-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-72: CTCKVHFPDPNKLHCFQLTVSPD → Y

Note: No experimental confirmation available.

Show »
Length:163
Mass (Da):18,631
Checksum:i85F172287CCF8D5A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811K → Q in DY311915 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei50 – 7223CTCKV…TVSPD → Y in isoform 2. 1 PublicationVSP_037303Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DY311915 mRNA. No translation available.
BC086355 mRNA. Translation: AAH86355.1.
RefSeqiNP_001008382.1. NM_001008381.1. [Q5U203-2]
XP_006245484.1. XM_006245422.2. [Q5U203-1]
XP_006245485.1. XM_006245423.2. [Q5U203-1]
XP_006245487.1. XM_006245425.2. [Q5U203-1]
XP_006245489.1. XM_006245427.2. [Q5U203-1]
XP_008765497.1. XM_008767275.1. [Q5U203-2]
UniGeneiRn.62010.

Genome annotation databases

EnsembliENSRNOT00000027050; ENSRNOP00000027050; ENSRNOG00000019953. [Q5U203-1]
ENSRNOT00000042082; ENSRNOP00000040354; ENSRNOG00000019953. [Q5U203-2]
GeneIDi363284.
KEGGirno:363284.
UCSCiRGD:1307608. rat. [Q5U203-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DY311915 mRNA. No translation available.
BC086355 mRNA. Translation: AAH86355.1.
RefSeqiNP_001008382.1. NM_001008381.1. [Q5U203-2]
XP_006245484.1. XM_006245422.2. [Q5U203-1]
XP_006245485.1. XM_006245423.2. [Q5U203-1]
XP_006245487.1. XM_006245425.2. [Q5U203-1]
XP_006245489.1. XM_006245427.2. [Q5U203-1]
XP_008765497.1. XM_008767275.1. [Q5U203-2]
UniGeneiRn.62010.

3D structure databases

ProteinModelPortaliQ5U203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027050.

Proteomic databases

PRIDEiQ5U203.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027050; ENSRNOP00000027050; ENSRNOG00000019953. [Q5U203-1]
ENSRNOT00000042082; ENSRNOP00000040354; ENSRNOG00000019953. [Q5U203-2]
GeneIDi363284.
KEGGirno:363284.
UCSCiRGD:1307608. rat. [Q5U203-1]

Organism-specific databases

CTDi140739.
RGDi1307608. Ube2f.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00630000089859.
HOGENOMiHOG000233456.
InParanoidiQ5U203.
KOiK10687.
OMAiWHPNISE.
OrthoDBiEOG7X9G8T.
PhylomeDBiQ5U203.
TreeFamiTF101125.

Enzyme and pathway databases

UniPathwayiUPA00885.

Miscellaneous databases

NextBioi683120.
PROiQ5U203.

Gene expression databases

GenevestigatoriQ5U203.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Ovary.

Entry informationi

Entry nameiUBE2F_RAT
AccessioniPrimary (citable) accession number: Q5U203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: April 1, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.