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Protein

1,5-anhydro-D-fructose reductase

Gene

Akr1e2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol.By similarity

Catalytic activityi

1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADPBy similarity
Active sitei40 – 401Proton donorBy similarity
Sitei69 – 691Lowers pKa of active site TyrBy similarity
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei174 – 1741NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi250 – 2589NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
1,5-anhydro-D-fructose reductase (EC:1.1.1.263)
Short name:
AF reductase
Alternative name(s):
Aldo-keto reductase family 1 member C-like protein 2
Aldo-keto reductase family 1 member E2
Gene namesi
Name:Akr1e2
Synonyms:Akr1cl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1309599. Akr1e2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3013011,5-anhydro-D-fructose reductasePRO_0000376896Add
BLAST

Proteomic databases

PaxDbiQ5U1Y4.
PRIDEiQ5U1Y4.

Expressioni

Gene expression databases

GenevisibleiQ5U1Y4. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023133.

Structurei

3D structure databases

ProteinModelPortaliQ5U1Y4.
SMRiQ5U1Y4. Positions 3-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
InParanoidiQ5U1Y4.
KOiK13981.
OMAiMERIPAV.
OrthoDBiEOG70KGQF.
PhylomeDBiQ5U1Y4.
TreeFamiTF106492.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5U1Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQIPTVGLG TWKASPGEVT DAVKVAINLG YRHFDCAYLY HNESEVGMGI
60 70 80 90 100
KEKIKEGVVK RDELFIVSKL WCTYHKQSLV KTACINTLEA LNLDYLDLYL
110 120 130 140 150
IHWPMGFKPG DKDIPLDRSG KVIPSHTSFL DTWEAMEDLV IEGLVKNIGV
160 170 180 190 200
SNFNHEQLDR LLNKPGLRIK PITNQIECHP YLNQKSLIDF CHGRNVSVTA
210 220 230 240 250
YRPLGGSRDG VHLMDDIVIR KIAKKHGKSP AQILIRFQIQ RNLIVIPKSV
260 270 280 290 300
NPSRIRENIQ VFDFELTEKD MEELLSLDKN LRLATFPSTE NHKDYPFHIE

Y
Length:301
Mass (Da):34,500
Last modified:December 7, 2004 - v1
Checksum:iF6786B806D5AE64B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086397 mRNA. Translation: AAH86397.1.
RefSeqiNP_001008343.1. NM_001008342.1.
UniGeneiRn.108718.

Genome annotation databases

EnsembliENSRNOT00000023133; ENSRNOP00000023133; ENSRNOG00000017165.
GeneIDi307091.
KEGGirno:307091.
UCSCiRGD:1309599. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086397 mRNA. Translation: AAH86397.1.
RefSeqiNP_001008343.1. NM_001008342.1.
UniGeneiRn.108718.

3D structure databases

ProteinModelPortaliQ5U1Y4.
SMRiQ5U1Y4. Positions 3-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023133.

Proteomic databases

PaxDbiQ5U1Y4.
PRIDEiQ5U1Y4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023133; ENSRNOP00000023133; ENSRNOG00000017165.
GeneIDi307091.
KEGGirno:307091.
UCSCiRGD:1309599. rat.

Organism-specific databases

CTDi83592.
RGDi1309599. Akr1e2.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
InParanoidiQ5U1Y4.
KOiK13981.
OMAiMERIPAV.
OrthoDBiEOG70KGQF.
PhylomeDBiQ5U1Y4.
TreeFamiTF106492.

Miscellaneous databases

PROiQ5U1Y4.

Gene expression databases

GenevisibleiQ5U1Y4. RN.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiAKCL2_RAT
AccessioniPrimary (citable) accession number: Q5U1Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: December 7, 2004
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.