Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-(apurinic or apyrimidinic site) lyase

Gene

APEX1

Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Probably binds two magnesium or manganese ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseUniRule annotation, Hydrolase, LyaseUniRule annotation, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
Gene namesi
Name:APEX1Imported
ORF Names:hCG_40473Imported
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Organism-specific databases

EuPathDBiHostDB:ENSG00000100823.11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, MitochondrionUniRule annotation, NucleusUniRule annotation

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA201059

PTM / Processingi

Proteomic databases

MaxQBiQ5TZP7
PRIDEiQ5TZP7

Expressioni

Gene expression databases

BgeeiENSG00000100823
ExpressionAtlasiQ5TZP7 baseline and differential

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

SMRiQ5TZP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 309Endo/exonuclease/phosphataseInterPro annotationAdd BLAST244

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1294 Eukaryota
COG0708 LUCA
HOVERGENiHBG050531
KOiK10771
OMAiGTAVFTK
OrthoDBiEOG091G0FDG

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR004808 AP_endonuc_1
IPR020847 AP_endonuclease_F1_BS
IPR020848 AP_endonuclease_F1_CS
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PANTHERiPTHR22748 PTHR22748, 1 hit
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
TIGRFAMsiTIGR00633 xth, 1 hit
PROSITEiView protein in PROSITE
PS00726 AP_NUCLEASE_F1_1, 1 hit
PS00727 AP_NUCLEASE_F1_2, 1 hit
PS00728 AP_NUCLEASE_F1_3, 1 hit
PS51435 AP_NUCLEASE_F1_4, 1 hit

Sequencei

Sequence statusi: Complete.

Q5TZP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD
60 70 80 90 100
QKTSPSGKPA TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS
110 120 130 140 150
ENKLPAELQE LPGLSHQYWS APSDKEGYSG VGLLSRQCPL KVSYGIGDEE
160 170 180 190 200
HDQEGRVIVA EFDSFVLVTA YVPNAGRGLV RLEYRQRWDE AFRKFLKGLA
210 220 230 240 250
SRKPLVLCGD LNVAHEEIDL RNPKGNKKNA GFTPQERQGF GELLQAVPLA
260 270 280 290 300
DSFRHLYPNT PYAYTFWTYM MNARSKNVGW RLDYFLLSHS LLPALCDSKI
310
RSKALGSDHC PITLYLAL
Length:318
Mass (Da):35,555
Last modified:May 10, 2005 - v1
Checksum:iB88579C01BAF80C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC095428 mRNA Translation: AAH95428.1
BT020133 mRNA Translation: AAV38935.1
AK291100 mRNA Translation: BAF83789.1
BX161454 mRNA Translation: CAD61917.1
CH471078 Genomic DNA Translation: EAW66463.1
CH471078 Genomic DNA Translation: EAW66464.1
CH471078 Genomic DNA Translation: EAW66465.1
CH471078 Genomic DNA Translation: EAW66466.1
CH471078 Genomic DNA Translation: EAW66467.1
CH471078 Genomic DNA Translation: EAW66468.1
RefSeqiNP_001231178.1, NM_001244249.1
NP_001632.2, NM_001641.3
NP_542379.1, NM_080648.2
NP_542380.1, NM_080649.2
UniGeneiHs.73722

Genome annotation databases

GeneIDi328
KEGGihsa:328
UCSCiuc001vxg.4 human

Similar proteinsi

Entry informationi

Entry nameiQ5TZP7_HUMAN
AccessioniPrimary (citable) accession number: Q5TZP7
Entry historyiIntegrated into UniProtKB/TrEMBL: May 10, 2005
Last sequence update: May 10, 2005
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health