Q5TZA2 (CROCC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rootletin

Alternative name(s):
Ciliary rootlet coiled-coil protein

Gene names

Name:	CROCC
Synonyms:	KIAA0445

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2017 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis. Ref.6 UniProtKB Q8CJ40
Subunit structure	Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250. Ref.6 UniProtKB Q8CJ40
Subcellular location	Cytoplasm › cytoskeleton › centrosome › centriole. Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase. Ref.5
Post-translational modification	Phosphorylated by NEK2 which may regulate its association with centrosomes. Ref.6
Sequence similarities	Belongs to the rootletin family.

Ontologies

Keywords
Biological process	Cell cycle Cilium biogenesis/degradation
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell projection organization Inferred from electronic annotation. Source: UniProtKB-KW centrosome organization Inferred from direct assay Ref.6. Source: UniProtKB protein localization Inferred from mutant phenotype PubMed 18086858. Source: UniProtKB
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA centriole Inferred from sequence or structural similarity. Source: UniProtKB ciliary rootlet Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	kinesin binding Inferred from sequence or structural similarity. Source: UniProtKB structural molecule activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.3 (identifier: Q5TZA2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.6 (identifier: Q5TZA2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-697: Missing.
     1984-1990: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2017	2017	Rootletin		PRO_0000239943
Regions
	Coiled coil	70 – 262	193	Potential
	Coiled coil	318 – 444	127	Potential
	Coiled coil	546 – 1058	513	Potential
	Coiled coil	1091 – 1438	348	Potential
	Coiled coil	1505 – 1704	200	Potential
	Compositional bias	1921 – 1999	79	Gln-rich
Amino acid modifications
	Modified residue	1460	1	Phosphoserine Ref.7
	Modified residue	1660	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 697	697	Missing in isoform 2. Ref.6		VSP_052069
	Alternative sequence	1984 – 1990	7	Missing in isoform 2. Ref.6		VSP_052070
	Natural variant	7	1	R → G. Corresponds to variant rs6586566 [ dbSNP | Ensembl ].		VAR_061626
	Natural variant	372	1	R → Q. Corresponds to variant rs57442576 [ dbSNP | Ensembl ].		VAR_061627
	Natural variant	439	1	A → V. Corresponds to variant rs4463721 [ dbSNP | Ensembl ].		VAR_059628
	Natural variant	586	1	D → H. Corresponds to variant rs9435714 [ dbSNP | Ensembl ].		VAR_059629
	Natural variant	1097	1	R → P. Corresponds to variant rs6669627 [ dbSNP | Ensembl ].		VAR_059630

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 9711AC13AD9D7C07	FASTA	2,017	228,523
	10 20 30 40 50 60 MSLGLARAQE VELTLETVIQ TLESSVLCQE KGLGARDLAQ DAQITSLPAL IREIVTRNLS 70 80 90 100 110 120 QPESPVLLPA TEMASLLSLQ EENQLLQQEL SRVEDLLAQS RAERDELAIK YNAVSERLEQ 130 140 150 160 170 180 ALRLEPGELE TQEPRGLVRQ SVELRRQLQE EQASYRRKLQ AYQEGQQRQA QLVQRLQGKI 190 200 210 220 230 240 LQYKKRCSEL EQQLLERSGE LEQQRLRDTE HSQDLESALI RLEEEQQRSA SLAQVNAMLR 250 260 270 280 290 300 EQLDQAGSAN QALSEDIRKV TNDWTRCRKE LEHREAAWRR EEESFNAYFS NEHSRLLLLW 310 320 330 340 350 360 RQVVGFRRLV SEVKMFTERD LLQLGGELAR TSRAVQEAGL GLSTGLRLAE SRAEAALEKQ 370 380 390 400 410 420 ALLQAQLEEQ LRDKVLREKD LAQQQMQSDL DKADLSARVT ELGLAVKRLE KQNLEKDQVN 430 440 450 460 470 480 KDLTEKLEAL ESLRLQEQAA LETEDGEGLQ QTLRDLAQAV LSDSESGVQL SGSERTADAS 490 500 510 520 530 540 NGSLRGLSGQ RTPSPPRRSS PGRGRSPRRG PSPACSDSST LALIHSALHK RQLQVQDMRG 550 560 570 580 590 600 RYEASQDLLG TLRKQLSDSE SERRALEEQL QRLRDKTDGA MQAHEDAQRE VQRLRSANEL 610 620 630 640 650 660 LSREKSNLAH SLQVAQQQAE ELRQEREKLQ AAQEELRRQR DRLEEEQEDA VQDGARVRRE 670 680 690 700 710 720 LERSHRQLEQ LEGKRSVLAK ELVEVREALS RATLQRDMLQ AEKAEVAEAL TKAEAGRVEL 730 740 750 760 770 780 ELSMTKLRAE EASLQDSLSK LSALNESLAQ DKLDLNRLVA QLEEEKSALQ GRQRQAEQEA 790 800 810 820 830 840 TVAREEQERL EELRLEQEVA RQGLEGSLRV AEQAQEALEQ QLPTLRHERS QLQEQLAQLS 850 860 870 880 890 900 RQLSGREQEL EQARREAQRQ VEALERAARE KEALAKEHAG LAVQLVAAER EGRTLSEEAT 910 920 930 940 950 960 RLRLEKEALE GSLFEVQRQL AQLEARREQL EAEGQALLLA KETLTGELAG LRQQIIATQE 970 980 990 1000 1010 1020 KASLDKELMA QKLVQAEREA QASLREQRAA HEEDLQRLQR EKEAAWRELE AERAQLQSQL 1030 1040 1050 1060 1070 1080 QREQEELLAR LEAEKEELSE EIAALQQERD EGLLLAESEK QQALSLKESE KTALSEKLMG 1090 1100 1110 1120 1130 1140 TRHSLATISL EMERQKRDAQ SRQEQDRSTV NALTSELRDL RAQREEAAAA HAQEVRRLQE 1150 1160 1170 1180 1190 1200 QARDLGKQRD SCLREAEELR TQLRLLEDAR DGLRRELLEA QRKLRESQEG REVQRQEAGE 1210 1220 1230 1240 1250 1260 LRRSLGEGAK EREALRRSNE ELRSAVKKAE SERISLKLAN EDKEQKLALL EEARTAVGKE 1270 1280 1290 1300 1310 1320 AGELRTGLQE VERSRLEARR ELQELRRQMK MLDSENTRLG RELAELQGRL ALGERAEKES 1330 1340 1350 1360 1370 1380 RRETLGLRQR LLKGEASLEV MRQELQVAQR KLQEQEGEFR TRERRLLGSL EEARGTEKQQ 1390 1400 1410 1420 1430 1440 LDHARGLELK LEAARAEAAE LGLRLSAAEG RAQGLEAELA RVEVQRRAAE AQLGGLRSAL 1450 1460 1470 1480 1490 1500 RRGLGLGRAP SPAPRPVPGS PARDAPAEGS GEGLNSPSTL ECSPGSQPPS PGPATSPASP 1510 1520 1530 1540 1550 1560 DLDPEAVRGA LREFLQELRS AQRERDELRT QTSALNRQLA EMEAERDSAT SRARQLQKAV 1570 1580 1590 1600 1610 1620 AESEEARRSV DGRLSGVQAE LALQEESVRR SERERRATLD QVATLERSLQ ATESELRASQ 1630 1640 1650 1660 1670 1680 EKISKMKANE TKLEGDKRRL KEVLDASESR TVKLELQRRS LEGELQRSRL GLSDREAQAQ 1690 1700 1710 1720 1730 1740 ALQDRVDSLQ RQVADSEVKA GTLQLTVERL NGALAKVEES EGALRDKVRG LTEALAQSSA 1750 1760 1770 1780 1790 1800 SLNSTRDKNL HLQKALTACE HDRQVLQERL DAARQALSEA RKQSSSLGEQ VQTLRGEVAD 1810 1820 1830 1840 1850 1860 LELQRVEAEG QLQQLREVLR QRQEGEAAAL NTVQKLQDER RLLQERLGSL QRALAQLEAE 1870 1880 1890 1900 1910 1920 KREVERSALR LEKDRVALRR TLDKVEREKL RSHEDTVRLS AEKGRLDRTL TGAELELAEA 1930 1940 1950 1960 1970 1980 QRQIQQLEAQ VVVLEQSHSP AQLEVDAQQQ QLELQQEVER LRSAQAQTER TLEARERAHR 1990 2000 2010 QRVRGLEEQV STLKGQLQQE LRRSSAPFSP PSGPPEK
	Isoform 2 [UniParc]. Checksum: 00DC42FDE419EED1	FASTA	1,313	148,256
	10 20 30 40 50 60 MLQAEKAEVA EALTKAEAGR VELELSMTKL RAEEASLQDS LSKLSALNES LAQDKLDLNR 70 80 90 100 110 120 LVAQLEEEKS ALQGRQRQAE QEATVAREEQ ERLEELRLEQ EVARQGLEGS LRVAEQAQEA 130 140 150 160 170 180 LEQQLPTLRH ERSQLQEQLA QLSRQLSGRE QELEQARREA QRQVEALERA AREKEALAKE 190 200 210 220 230 240 HAGLAVQLVA AEREGRTLSE EATRLRLEKE ALEGSLFEVQ RQLAQLEARR EQLEAEGQAL 250 260 270 280 290 300 LLAKETLTGE LAGLRQQIIA TQEKASLDKE LMAQKLVQAE REAQASLREQ RAAHEEDLQR 310 320 330 340 350 360 LQREKEAAWR ELEAERAQLQ SQLQREQEEL LARLEAEKEE LSEEIAALQQ ERDEGLLLAE 370 380 390 400 410 420 SEKQQALSLK ESEKTALSEK LMGTRHSLAT ISLEMERQKR DAQSRQEQDR STVNALTSEL 430 440 450 460 470 480 RDLRAQREEA AAAHAQEVRR LQEQARDLGK QRDSCLREAE ELRTQLRLLE DARDGLRREL 490 500 510 520 530 540 LEAQRKLRES QEGREVQRQE AGELRRSLGE GAKEREALRR SNEELRSAVK KAESERISLK 550 560 570 580 590 600 LANEDKEQKL ALLEEARTAV GKEAGELRTG LQEVERSRLE ARRELQELRR QMKMLDSENT 610 620 630 640 650 660 RLGRELAELQ GRLALGERAE KESRRETLGL RQRLLKGEAS LEVMRQELQV AQRKLQEQEG 670 680 690 700 710 720 EFRTRERRLL GSLEEARGTE KQQLDHARGL ELKLEAARAE AAELGLRLSA AEGRAQGLEA 730 740 750 760 770 780 ELARVEVQRR AAEAQLGGLR SALRRGLGLG RAPSPAPRPV PGSPARDAPA EGSGEGLNSP 790 800 810 820 830 840 STLECSPGSQ PPSPGPATSP ASPDLDPEAV RGALREFLQE LRSAQRERDE LRTQTSALNR 850 860 870 880 890 900 QLAEMEAERD SATSRARQLQ KAVAESEEAR RSVDGRLSGV QAELALQEES VRRSERERRA 910 920 930 940 950 960 TLDQVATLER SLQATESELR ASQEKISKMK ANETKLEGDK RRLKEVLDAS ESRTVKLELQ 970 980 990 1000 1010 1020 RRSLEGELQR SRLGLSDREA QAQALQDRVD SLQRQVADSE VKAGTLQLTV ERLNGALAKV 1030 1040 1050 1060 1070 1080 EESEGALRDK VRGLTEALAQ SSASLNSTRD KNLHLQKALT ACEHDRQVLQ ERLDAARQAL 1090 1100 1110 1120 1130 1140 SEARKQSSSL GEQVQTLRGE VADLELQRVE AEGQLQQLRE VLRQRQEGEA AALNTVQKLQ 1150 1160 1170 1180 1190 1200 DERRLLQERL GSLQRALAQL EAEKREVERS ALRLEKDRVA LRRTLDKVER EKLRSHEDTV 1210 1220 1230 1240 1250 1260 RLSAEKGRLD RTLTGAELEL AEAQRQIQQL EAQVVVLEQS HSPAQLEVDA QQQQLELQQE 1270 1280 1290 1300 1310 VERLRSAQAQ TERTLEARER AHRQRVSTLK GQLQQELRRS SAPFSPPSGP PEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A centrosomal target of human T-cell leukemia virus type 1 oncoprotein Tax." Ching Y.-P., Chan S.-F., Jeang K.-T., Jin D.-Y. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 99-2017 (ISOFORM 1). Tissue: Brain.
[4]	"Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries." Behrends U., Schneider I., Roessler S., Frauenknecht H., Golbeck A., Lechner B., Eigenstetter G., Zobywalski C., Mueller-Weihrich S., Graubner U., Schmid I., Sackerer D., Spaeth M., Goetz C., Prantl F., Asmuss H.-P., Bise K., Mautner J. Int. J. Cancer 106:244-251(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1572-2017 (ISOFORM 2). Tissue: Medulloblastoma.
[5]	"Proteomic characterization of the human centrosome by protein correlation profiling." Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M. Nature 426:570-574(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Tissue: Lymphoblast.
[6]	"Rootletin forms centriole-associated filaments and functions in centrosome cohesion." Bahe S., Stierhof Y.-D., Wilkinson C.J., Leiss F., Nigg E.A. J. Cell Biol. 171:27-33(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CEP250 AND NEK2, PHOSPHORYLATION.
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1460 AND SER-1660, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ139275 mRNA. Translation: ABA43896.1. BX284668, AL049569 Genomic DNA. Translation: CAH70055.1. AL049569, BX284668 Genomic DNA. Translation: CAI20363.1. AB007914 mRNA. Translation: BAA32290.2. AF527734 mRNA. Translation: AAP85633.1. BK005505 mRNA. Translation: DAA05505.1.
IPI	IPI00456492. IPI00644892.
RefSeq	NP_055490.3. NM_014675.3.
UniGene	Hs.309403.
3D structure databases
ProteinModelPortal	Q5TZA2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5TZA2. 2 interactions.
MINT	MINT-2807877.
STRING	9606.ENSP00000364691.
PTM databases
PhosphoSite	Q5TZA2.
Polymorphism databases
DMDM	74746681.
Proteomic databases
PaxDb	Q5TZA2.
PRIDE	Q5TZA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375541; ENSP00000364691; ENSG00000058453.
GeneID	9696.
KEGG	hsa:9696.
UCSC	uc001azt.2. human. uc001azu.2. human.
Organism-specific databases
CTD	9696.
GeneCards	GC01P017223.
H-InvDB	HIX0000171. HIX0000176.
HGNC	HGNC:21299. CROCC.
HPA	HPA021191. HPA021762.
neXtProt	NX_Q5TZA2.
PharmGKB	PA134911945.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000231187.
HOVERGEN	HBG080162.
InParanoid	Q5TZA2.
KO	K16469.
OMA	GKQRDSC.
OrthoDB	EOG4BK531.
Gene expression databases
ArrayExpress	Q5TZA2.
Bgee	Q5TZA2.
CleanEx	HS_CROCC.
Genevestigator	Q5TZA2.
GermOnline	ENSG00000058453. Homo sapiens.
Family and domain databases
InterPro	IPR026733. Rootletin. [Graphical view]
PANTHER	PTHR23159:SF5. PTHR23159:SF5. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	CROCC. human.
GenomeRNAi	9696.
NextBio	36431.

Entry information

Entry name

CROCC_HUMAN

Accession

Primary (citable) accession number: Q5TZA2
Secondary accession number(s): Q2VHY3 , Q66GT7, Q7Z2L4, Q7Z5D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	December 7, 2004
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.