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Q5TZA2 (CROCC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rootletin
Alternative name(s):
Ciliary rootlet coiled-coil protein
Gene names
Name:CROCC
Synonyms:KIAA0445
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2017 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis. Ref.6 UniProtKB Q8CJ40

Subunit structure

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250. Ref.6 UniProtKB Q8CJ40

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase. Ref.5

Post-translational modification

Phosphorylated by NEK2 which may regulate its association with centrosomes. Ref.6

Sequence similarities

Belongs to the rootletin family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.3 (identifier: Q5TZA2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.6 (identifier: Q5TZA2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-697: Missing.
     1984-1990: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20172017Rootletin
PRO_0000239943

Regions

Coiled coil70 – 262193 Potential
Coiled coil318 – 444127 Potential
Coiled coil546 – 1058513 Potential
Coiled coil1091 – 1438348 Potential
Coiled coil1505 – 1704200 Potential
Compositional bias1921 – 199979Gln-rich

Amino acid modifications

Modified residue14601Phosphoserine Ref.7
Modified residue16601Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 697697Missing in isoform 2. Ref.6
VSP_052069
Alternative sequence1984 – 19907Missing in isoform 2. Ref.6
VSP_052070
Natural variant71R → G.
Corresponds to variant rs6586566 [ dbSNP | Ensembl ].
VAR_061626
Natural variant3721R → Q.
Corresponds to variant rs57442576 [ dbSNP | Ensembl ].
VAR_061627
Natural variant4391A → V.
Corresponds to variant rs4463721 [ dbSNP | Ensembl ].
VAR_059628
Natural variant5861D → H.
Corresponds to variant rs9435714 [ dbSNP | Ensembl ].
VAR_059629
Natural variant10971R → P.
Corresponds to variant rs6669627 [ dbSNP | Ensembl ].
VAR_059630

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 9711AC13AD9D7C07

FASTA2,017228,523
        10         20         30         40         50         60 
MSLGLARAQE VELTLETVIQ TLESSVLCQE KGLGARDLAQ DAQITSLPAL IREIVTRNLS 

        70         80         90        100        110        120 
QPESPVLLPA TEMASLLSLQ EENQLLQQEL SRVEDLLAQS RAERDELAIK YNAVSERLEQ 

       130        140        150        160        170        180 
ALRLEPGELE TQEPRGLVRQ SVELRRQLQE EQASYRRKLQ AYQEGQQRQA QLVQRLQGKI 

       190        200        210        220        230        240 
LQYKKRCSEL EQQLLERSGE LEQQRLRDTE HSQDLESALI RLEEEQQRSA SLAQVNAMLR 

       250        260        270        280        290        300 
EQLDQAGSAN QALSEDIRKV TNDWTRCRKE LEHREAAWRR EEESFNAYFS NEHSRLLLLW 

       310        320        330        340        350        360 
RQVVGFRRLV SEVKMFTERD LLQLGGELAR TSRAVQEAGL GLSTGLRLAE SRAEAALEKQ 

       370        380        390        400        410        420 
ALLQAQLEEQ LRDKVLREKD LAQQQMQSDL DKADLSARVT ELGLAVKRLE KQNLEKDQVN 

       430        440        450        460        470        480 
KDLTEKLEAL ESLRLQEQAA LETEDGEGLQ QTLRDLAQAV LSDSESGVQL SGSERTADAS 

       490        500        510        520        530        540 
NGSLRGLSGQ RTPSPPRRSS PGRGRSPRRG PSPACSDSST LALIHSALHK RQLQVQDMRG 

       550        560        570        580        590        600 
RYEASQDLLG TLRKQLSDSE SERRALEEQL QRLRDKTDGA MQAHEDAQRE VQRLRSANEL 

       610        620        630        640        650        660 
LSREKSNLAH SLQVAQQQAE ELRQEREKLQ AAQEELRRQR DRLEEEQEDA VQDGARVRRE 

       670        680        690        700        710        720 
LERSHRQLEQ LEGKRSVLAK ELVEVREALS RATLQRDMLQ AEKAEVAEAL TKAEAGRVEL 

       730        740        750        760        770        780 
ELSMTKLRAE EASLQDSLSK LSALNESLAQ DKLDLNRLVA QLEEEKSALQ GRQRQAEQEA 

       790        800        810        820        830        840 
TVAREEQERL EELRLEQEVA RQGLEGSLRV AEQAQEALEQ QLPTLRHERS QLQEQLAQLS 

       850        860        870        880        890        900 
RQLSGREQEL EQARREAQRQ VEALERAARE KEALAKEHAG LAVQLVAAER EGRTLSEEAT 

       910        920        930        940        950        960 
RLRLEKEALE GSLFEVQRQL AQLEARREQL EAEGQALLLA KETLTGELAG LRQQIIATQE 

       970        980        990       1000       1010       1020 
KASLDKELMA QKLVQAEREA QASLREQRAA HEEDLQRLQR EKEAAWRELE AERAQLQSQL 

      1030       1040       1050       1060       1070       1080 
QREQEELLAR LEAEKEELSE EIAALQQERD EGLLLAESEK QQALSLKESE KTALSEKLMG 

      1090       1100       1110       1120       1130       1140 
TRHSLATISL EMERQKRDAQ SRQEQDRSTV NALTSELRDL RAQREEAAAA HAQEVRRLQE 

      1150       1160       1170       1180       1190       1200 
QARDLGKQRD SCLREAEELR TQLRLLEDAR DGLRRELLEA QRKLRESQEG REVQRQEAGE 

      1210       1220       1230       1240       1250       1260 
LRRSLGEGAK EREALRRSNE ELRSAVKKAE SERISLKLAN EDKEQKLALL EEARTAVGKE 

      1270       1280       1290       1300       1310       1320 
AGELRTGLQE VERSRLEARR ELQELRRQMK MLDSENTRLG RELAELQGRL ALGERAEKES 

      1330       1340       1350       1360       1370       1380 
RRETLGLRQR LLKGEASLEV MRQELQVAQR KLQEQEGEFR TRERRLLGSL EEARGTEKQQ 

      1390       1400       1410       1420       1430       1440 
LDHARGLELK LEAARAEAAE LGLRLSAAEG RAQGLEAELA RVEVQRRAAE AQLGGLRSAL 

      1450       1460       1470       1480       1490       1500 
RRGLGLGRAP SPAPRPVPGS PARDAPAEGS GEGLNSPSTL ECSPGSQPPS PGPATSPASP 

      1510       1520       1530       1540       1550       1560 
DLDPEAVRGA LREFLQELRS AQRERDELRT QTSALNRQLA EMEAERDSAT SRARQLQKAV 

      1570       1580       1590       1600       1610       1620 
AESEEARRSV DGRLSGVQAE LALQEESVRR SERERRATLD QVATLERSLQ ATESELRASQ 

      1630       1640       1650       1660       1670       1680 
EKISKMKANE TKLEGDKRRL KEVLDASESR TVKLELQRRS LEGELQRSRL GLSDREAQAQ 

      1690       1700       1710       1720       1730       1740 
ALQDRVDSLQ RQVADSEVKA GTLQLTVERL NGALAKVEES EGALRDKVRG LTEALAQSSA 

      1750       1760       1770       1780       1790       1800 
SLNSTRDKNL HLQKALTACE HDRQVLQERL DAARQALSEA RKQSSSLGEQ VQTLRGEVAD 

      1810       1820       1830       1840       1850       1860 
LELQRVEAEG QLQQLREVLR QRQEGEAAAL NTVQKLQDER RLLQERLGSL QRALAQLEAE 

      1870       1880       1890       1900       1910       1920 
KREVERSALR LEKDRVALRR TLDKVEREKL RSHEDTVRLS AEKGRLDRTL TGAELELAEA 

      1930       1940       1950       1960       1970       1980 
QRQIQQLEAQ VVVLEQSHSP AQLEVDAQQQ QLELQQEVER LRSAQAQTER TLEARERAHR 

      1990       2000       2010 
QRVRGLEEQV STLKGQLQQE LRRSSAPFSP PSGPPEK 

« Hide

Isoform 2 [UniParc].

Checksum: 00DC42FDE419EED1
Show »

FASTA1,313148,256

References

« Hide 'large scale' references
[1]"A centrosomal target of human T-cell leukemia virus type 1 oncoprotein Tax."
Ching Y.-P., Chan S.-F., Jeang K.-T., Jin D.-Y.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O.
DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 99-2017 (ISOFORM 1).
Tissue: Brain.
[4]"Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries."
Behrends U., Schneider I., Roessler S., Frauenknecht H., Golbeck A., Lechner B., Eigenstetter G., Zobywalski C., Mueller-Weihrich S., Graubner U., Schmid I., Sackerer D., Spaeth M., Goetz C., Prantl F., Asmuss H.-P., Bise K., Mautner J.
Int. J. Cancer 106:244-251(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1572-2017 (ISOFORM 2).
Tissue: Medulloblastoma.
[5]"Proteomic characterization of the human centrosome by protein correlation profiling."
Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.
Nature 426:570-574(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Lymphoblast.
[6]"Rootletin forms centriole-associated filaments and functions in centrosome cohesion."
Bahe S., Stierhof Y.-D., Wilkinson C.J., Leiss F., Nigg E.A.
J. Cell Biol. 171:27-33(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CEP250 AND NEK2, PHOSPHORYLATION.
[7]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1460 AND SER-1660, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ139275 mRNA. Translation: ABA43896.1.
BX284668, AL049569 Genomic DNA. Translation: CAH70055.1.
AL049569, BX284668 Genomic DNA. Translation: CAI20363.1.
AB007914 mRNA. Translation: BAA32290.2.
AF527734 mRNA. Translation: AAP85633.1.
BK005505 mRNA. Translation: DAA05505.1.
RefSeqNP_055490.4. NM_014675.4. [Q5TZA2-1]
UniGeneHs.309403.

3D structure databases

ProteinModelPortalQ5TZA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115048. 6 interactions.
IntActQ5TZA2. 4 interactions.
MINTMINT-2807877.
STRING9606.ENSP00000364691.

PTM databases

PhosphoSiteQ5TZA2.

Polymorphism databases

DMDM74746681.

Proteomic databases

MaxQBQ5TZA2.
PaxDbQ5TZA2.
PRIDEQ5TZA2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000375541; ENSP00000364691; ENSG00000058453. [Q5TZA2-1]
GeneID9696.
KEGGhsa:9696.
UCSCuc001azt.2. human. [Q5TZA2-1]
uc001azu.2. human. [Q5TZA2-2]

Organism-specific databases

CTD9696.
GeneCardsGC01P017093.
H-InvDBHIX0000171.
HIX0000176.
HGNCHGNC:21299. CROCC.
HPAHPA021191.
HPA021762.
neXtProtNX_Q5TZA2.
PharmGKBPA134911945.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000231187.
HOVERGENHBG080162.
InParanoidQ5TZA2.
KOK16469.
OMAYKKRCSE.
OrthoDBEOG7W6WJX.
PhylomeDBQ5TZA2.
TreeFamTF101138.

Gene expression databases

BgeeQ5TZA2.
CleanExHS_CROCC.
GenevestigatorQ5TZA2.

Family and domain databases

InterProIPR026733. Rootletin.
[Graphical view]
PANTHERPTHR23159:SF11. PTHR23159:SF11. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCROCC. human.
GeneWikiRootletin.
GenomeRNAi9696.
NextBio36431.
PROQ5TZA2.

Entry information

Entry nameCROCC_HUMAN
AccessionPrimary (citable) accession number: Q5TZA2
Secondary accession number(s): Q2VHY3 expand/collapse secondary AC list , Q66GT7, Q7Z2L4, Q7Z5D7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM