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Protein

Homeobox protein NANOG

Gene

NANOG

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein NANOG
Alternative name(s):
Homeobox transcription factor Nanog
Gene namesi
Name:NANOG
Synonyms:STM1
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Homeobox protein NANOGPRO_0000261421Add
BLAST

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Interactioni

Subunit structurei

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281. Interacts with PCGF1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5TM84.
SMRiQ5TM84. Positions 100-154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati196 – 20051
Repeati201 – 20552
Repeati206 – 21053
Repeati216 – 22054
Repeati221 – 22555
Repeati226 – 23056
Repeati231 – 23557
Repeati236 – 24058

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni196 – 240458 X repeats starting with a Trp in each unitAdd
BLAST
Regioni196 – 24045Sufficient for transactivation activityBy similarityAdd
BLAST
Regioni241 – 30565Sufficient for strong transactivation activityBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the Nanog homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, Repeat

Phylogenomic databases

HOVERGENiHBG058783.
KOiK10164.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5TM84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVDPACPQS LPCLEASDSK ESSPMPVICG PEENYPSLQM SSAEMPHTET
60 70 80 90 100
VSPLPSSMDL LIQDSPDSST SPKGKQPTAA ENSATKKEDK VPVKKQKART
110 120 130 140 150
VFSSAQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS
160 170 180 190 200
KRWQKNNWPK NSNGVTQKAS APTYPSLYSS CHQGCLVNPT GNLPMWSNQT
210 220 230 240 250
WNNSSWSNQT QNIQSWSNHS WNAQTWCTQS WNNQAWNSPF SNCGEESLQS
260 270 280 290 300
CLQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYLSTPQTV DLLLNYSTNM

QPEDV
Length:305
Mass (Da):34,153
Last modified:December 21, 2004 - v1
Checksum:i3FC6FD226DF5E998
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB126938 mRNA. Translation: BAD72891.1.
RefSeqiNP_001274577.1. NM_001287648.1.
UniGeneiMfa.5359.

Genome annotation databases

GeneIDi102114964.
KEGGimcf:102114964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB126938 mRNA. Translation: BAD72891.1.
RefSeqiNP_001274577.1. NM_001287648.1.
UniGeneiMfa.5359.

3D structure databases

ProteinModelPortaliQ5TM84.
SMRiQ5TM84. Positions 100-154.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102114964.
KEGGimcf:102114964.

Organism-specific databases

CTDi79923.

Phylogenomic databases

HOVERGENiHBG058783.
KOiK10164.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANOG_MACFA
AccessioniPrimary (citable) accession number: Q5TM84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.