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Q5TM49

- PO5F1_MACMU

UniProt

Q5TM49 - PO5F1_MACMU

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Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi230 – 28960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
ProteomesiUP000006718: Unplaced

Subcellular locationi

Cytoplasm. Nucleus
Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360POU domain, class 5, transcription factor 1PRO_0000100748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity
Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei235 – 2351PhosphothreonineBy similarity
Modified residuei236 – 2361PhosphoserineBy similarity
Modified residuei289 – 2891PhosphoserineBy similarity
Modified residuei290 – 2901PhosphoserineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).By similarity
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000020598.

Structurei

3D structure databases

ProteinModelPortaliQ5TM49.
SMRiQ5TM49. Positions 144-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 21275POU-specificPROSITE-ProRule annotationAdd
BLAST

Domaini

The POU-specific domain mediates interaction with PKM.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiQ5TM49.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5TM49-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGHLASDFA FSPPPGGGGD GPGGPETGWV DPRTWLSFQG PPGGPGIGPG
60 70 80 90 100
VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG
110 120 130 140 150
AGVESNSDGA SPEPCTVPTG AVKLEKEKLE QNPEESQDIK ALQKELEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGSLENLFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA
310 320 330 340 350
AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVPV
360
TTLGSPMHSN
Length:360
Mass (Da):38,530
Last modified:December 21, 2004 - v1
Checksum:iC41EDDB07A3980AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128049 Genomic DNA. Translation: BAD69745.1.
RefSeqiNP_001108427.1. NM_001114955.1.
UniGeneiMmu.17468.

Genome annotation databases

GeneIDi714760.
KEGGimcc:714760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128049 Genomic DNA. Translation: BAD69745.1 .
RefSeqi NP_001108427.1. NM_001114955.1.
UniGenei Mmu.17468.

3D structure databases

ProteinModelPortali Q5TM49.
SMRi Q5TM49. Positions 144-289.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9544.ENSMMUP00000020598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 714760.
KEGGi mcc:714760.

Organism-specific databases

CTDi 5460.

Phylogenomic databases

eggNOGi NOG329627.
HOGENOMi HOG000089941.
HOVERGENi HBG057998.
InParanoidi Q5TM49.
KOi K09367.

Miscellaneous databases

NextBioi 19972540.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rhesus macaque class I duplicon structures, organization, and evolution within the alpha block of the major histocompatibility complex."
    Kulski J.K., Anzai T., Shiina T., Inoko H.
    Mol. Biol. Evol. 21:2079-2091(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts."
    Liu H., Zhu F., Yong J., Zhang P., Hou P., Li H., Jiang W., Cai J., Liu M., Cui K., Qu X., Xiang T., Lu D., Chi X., Gao G., Ji W., Ding M., Deng H.
    Cell Stem Cell 3:587-590(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY, FUNCTION.

Entry informationi

Entry nameiPO5F1_MACMU
AccessioniPrimary (citable) accession number: Q5TM49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 21, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3