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Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi230 – 289HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007481 – 360POU domain, class 5, transcription factor 1Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine; by MAPKBy similarity1
Cross-linki123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei235PhosphothreonineBy similarity1
Modified residuei236PhosphoserineBy similarity1
Modified residuei289PhosphoserineBy similarity1
Modified residuei290PhosphoserineBy similarity1
Modified residuei355PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).By similarity
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2. Interacts with PCGF1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000020598.

Structurei

3D structure databases

ProteinModelPortaliQ5TM49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 212POU-specificPROSITE-ProRule annotationAdd BLAST75

Domaini

The POU-specific domain mediates interaction with PKM.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiQ5TM49.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
[Graphical view]
PANTHERiPTHR11636:SF86. PTHR11636:SF86. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5TM49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGHLASDFA FSPPPGGGGD GPGGPETGWV DPRTWLSFQG PPGGPGIGPG
60 70 80 90 100
VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG
110 120 130 140 150
AGVESNSDGA SPEPCTVPTG AVKLEKEKLE QNPEESQDIK ALQKELEQFA
160 170 180 190 200
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL
210 220 230 240 250
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGSLENLFL
260 270 280 290 300
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA
310 320 330 340 350
AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVPV
360
TTLGSPMHSN
Length:360
Mass (Da):38,530
Last modified:December 21, 2004 - v1
Checksum:iC41EDDB07A3980AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128049 Genomic DNA. Translation: BAD69745.1.
RefSeqiNP_001108427.1. NM_001114955.1.
UniGeneiMmu.17468.

Genome annotation databases

GeneIDi714760.
KEGGimcc:714760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB128049 Genomic DNA. Translation: BAD69745.1.
RefSeqiNP_001108427.1. NM_001114955.1.
UniGeneiMmu.17468.

3D structure databases

ProteinModelPortaliQ5TM49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000020598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi714760.
KEGGimcc:714760.

Organism-specific databases

CTDi5460.

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiQ5TM49.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR015585. POU_dom_5.
[Graphical view]
PANTHERiPTHR11636:SF86. PTHR11636:SF86. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO5F1_MACMU
AccessioniPrimary (citable) accession number: Q5TM49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 21, 2004
Last modified: October 5, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.