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Q5TM49 (PO5F1_MACMU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name=Oct-3
Octamer-binding protein 4
Short name=Oct-4
Octamer-binding transcription factor 3
Short name=OTF-3
Gene names
Name:POU5F1
Synonyms:OCT3, OCT4
OrganismMacaca mulatta (Rhesus macaque) [Reference proteome]
Taxonomic identifier9544 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. Ref.2

Subunit structure

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.

Subcellular location

Cytoplasm. Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern By similarity.

Domain

The POU-specific domain mediates interaction with PKM By similarity.

Post-translational modification

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.

Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation By similarity.

ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.

Biotechnological use

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360POU domain, class 5, transcription factor 1
PRO_0000100748

Regions

Domain138 – 21275POU-specific
DNA binding230 – 28960Homeobox

Amino acid modifications

Modified residue1111Phosphoserine; by MAPK By similarity
Modified residue2351Phosphothreonine By similarity
Modified residue2361Phosphoserine By similarity
Modified residue2891Phosphoserine By similarity
Modified residue2901Phosphoserine By similarity
Modified residue3551Phosphoserine By similarity
Cross-link123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5TM49 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: C41EDDB07A3980AF

FASTA36038,530
        10         20         30         40         50         60 
MAGHLASDFA FSPPPGGGGD GPGGPETGWV DPRTWLSFQG PPGGPGIGPG VGPGSEVWGI 

        70         80         90        100        110        120 
PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG AGVESNSDGA SPEPCTVPTG 

       130        140        150        160        170        180 
AVKLEKEKLE QNPEESQDIK ALQKELEQFA KLLKQKRITL GYTQADVGLT LGVLFGKVFS 

       190        200        210        220        230        240 
QTTICRFEAL QLSFKNMCKL RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR 

       250        260        270        280        290        300 
VRGSLENLFL QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA 

       310        320        330        340        350        360 
AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVPV TTLGSPMHSN 

« Hide

References

« Hide 'large scale' references
[1]"Rhesus macaque class I duplicon structures, organization, and evolution within the alpha block of the major histocompatibility complex."
Kulski J.K., Anzai T., Shiina T., Inoko H.
Mol. Biol. Evol. 21:2079-2091(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts."
Liu H., Zhu F., Yong J., Zhang P., Hou P., Li H., Jiang W., Cai J., Liu M., Cui K., Qu X., Xiang T., Lu D., Chi X., Gao G., Ji W., Ding M., Deng H.
Cell Stem Cell 3:587-590(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB128049 Genomic DNA. Translation: BAD69745.1.
RefSeqNP_001108427.1. NM_001114955.1.
UniGeneMmu.17468.

3D structure databases

ProteinModelPortalQ5TM49.
SMRQ5TM49. Positions 144-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9544.ENSMMUP00000020598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID714760.
KEGGmcc:714760.

Organism-specific databases

CTD5460.

Phylogenomic databases

eggNOGNOG329627.
HOGENOMHOG000089941.
HOVERGENHBG057998.
InParanoidQ5TM49.
KOK09367.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio19972540.

Entry information

Entry namePO5F1_MACMU
AccessionPrimary (citable) accession number: Q5TM49
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: December 21, 2004
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families