Q5TLG6 (Q5TLG6_9CNID) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Fluorescent protein Dronpa EMBL BAD72874.1

Gene names

Name:

Dronpa EMBL BAD72874.1

Organism

Echinophyllia sp. SC22 EMBL BAD72874.1

Taxonomic identifier

301887 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Scleractinia › Faviina › Pectiniidae › Echinophyllia

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2IE2 PDB 2Z6Y PDB 2Z1O PDB 2Z6X PDB 2Z6Z PDB 2IOV PDB 2GX0 PDB 2GX2 PDB 2POX PDB 3ZUF PDB 3ZUJ PDB 3ZUL PDB 4EMQ
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: InterPro generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5TLG6 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 9091A477C9A99D98	FASTA	224	25,481
	10 20 30 40 50 60 MSVIKPDMKI KLRMEGAVNG HPFAIEGVGL GKPFEGKQSM DLKVKEGGPL PFAYDILTTV 70 80 90 100 110 120 FCYGNRVFAK YPENIVDYFK QSFPEGYSWE RSMNYEDGGI CNATNDITLD GDCYIYEIRF 130 140 150 160 170 180 DGVNFPANGP VMQKRTVKWE PSTEKLYVRD GVLKGDVNMA LSLEGGGHYR CDFKTTYKAK 190 200 210 220 KVVQLPDYHF VDHHIEIKSH DKDYSNVNLH EHAEAHSELP RQAK

References

[1]	"Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting." Ando R., Mizuno H., Miyawaki A. Science 306:1370-1373(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SC22 EMBL BAD72874.1.
[2]	"The 1.7 A crystal structure of Dronpa: a photoswitchable green fluorescent protein." Wilmann P.G., Turcic K., Battad J.M., Wilce M.C., Devenish R.J., Prescott M., Rossjohn J. J. Mol. Biol. 364:213-224(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
[3]	"Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S." Nam K.H., Kwon O.Y., Sugiyama K., Lee W.H., Kim Y.K., Song H.K., Kim E.E., Park S.Y., Jeon H., Hwang K.Y. Biochem. Biophys. Res. Commun. 354:962-967(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 2-224.
[4]	"1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants." Stiel A.C., Trowitzsch S., Weber G., Andresen M., Eggeling C., Hell S.W., Jakobs S., Wahl M.C. Biochem. J. 402:35-42(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
[5]	"Structural basis for reversible photoswitching in Dronpa." Andresen M., Stiel A.C., Trowitzsch S., Weber G., Eggeling C., Wahl M.C., Hell S.W., Jakobs S. Proc. Natl. Acad. Sci. U.S.A. 104:13005-13009(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
[6]	"Light-dependent regulation of structural flexibility in a photochromic fluorescent protein." Mizuno H., Mal T.K., Walchli M., Kikuchi A., Fukano T., Ando R., Jeyakanthan J., Taka J., Shiro Y., Ikura M., Miyawaki A. Proc. Natl. Acad. Sci. U.S.A. 105:9227-9232(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
[7]	"Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron." Faro A.R., Carpentier P., Jonasson G., Pompidor G., Arcizet D., Demachy I., Bourgeois D. J. Am. Chem. Soc. 133:16362-16365(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-218.
[8]	"Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa." Nguyen Bich N., Moeyaert B., Van Hecke K., Dedecker P., Mizuno H., Hofkens J., Van Meervelt L. Acta Crystallogr. D 68:1653-1659(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB180726 Genomic DNA. Translation: BAD72874.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GX0	X-ray	1.90	A/B/C/D	2-224	[»]
2GX2	X-ray	1.80	A/B/C/D/E/F/G/H/I/J/K/L	2-224	[»]
2IE2	X-ray	1.70	A/B/C/D/E/F	1-224	[»]
2IOV	X-ray	1.80	A/B/C/D	1-224	[»]
2POX	X-ray	1.95	A/B/C/D	1-224	[»]
2Z1O	X-ray	1.75	A/B/C/D	1-224	[»]
2Z6X	X-ray	2.30	A/B/C/D/E/F/G/H	1-224	[»]
2Z6Y	X-ray	2.00	A/B/C/D	1-224	[»]
2Z6Z	X-ray	1.80	A/B/C/D/E/F	1-224	[»]
3ZUF	X-ray	2.20	A/B/C/D/E/F	2-218	[»]
3ZUJ	X-ray	2.34	A/B/C/D/E/F	2-217	[»]
3ZUL	X-ray	2.30	A/B/C/D/E/F	2-224	[»]
4EMQ	X-ray	1.95	A/B/C/D/E/F	1-224	[»]

ProteinModelPortal

Q5TLG6.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46137N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.40.155.10. 1 hit.

InterPro

IPR009017. GFP.
IPR011584. GFP-related.
IPR023413. GFP_like.
IPR000786. Green_fluorescent_prot.
[Graphical view]

Pfam

PF01353. GFP. 1 hit.
[Graphical view]

PRINTS

PR01229. GFLUORESCENT.

SUPFAM

SSF54511. GFP_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q5TLG6.

Entry information

Entry name

Q5TLG6_9CNID

Accession

Primary (citable) accession number: Q5TLG6

Entry history

Integrated into UniProtKB/TrEMBL:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)