Q5TKA1 (LIN9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lin-9 homolog
Short name=HuLin-9
Short name=hLin-9

Alternative name(s):
Beta subunit-associated regulator of apoptosis
TUDOR gene similar protein
Type I interferon receptor beta chain-associated protein
pRB-associated protein

Gene names

Name:	LIN9
Synonyms:	BARA, TGS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a tumor suppressor. Inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. Plays a role in the expression of genes required for the G1/S transition. Ref.1 Ref.2
Subunit structure	Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RB1. Ref.1 Ref.6 Ref.7
Subcellular location	Nucleus › nucleoplasm. Note: Found in perinucleolar structures. Associated with chromatin. Ref.1 Ref.2
Tissue specificity	Expressed in thymus and testis. Ref.1
Sequence similarities	Belongs to the lin-9 family.
Sequence caution	The sequence AAH43444.1 differs from that shown. Reason: Erroneous initiation. The sequence CAD97871.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle DNA synthesis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Tumor suppressor
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW mitotic cell cycle Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5TKA1-1)

Also known as: Lin9-L; Long form;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5TKA1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MHRGGQPLKKRRGSFKM

Isoform 3 (identifier: Q5TKA1-3)

Also known as: Lin9-S; Short form;

The sequence of this isoform differs from the canonical sequence as follows:
     54-88: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Protein lin-9 homolog		PRO_0000249546
Regions
	Region	1 – 296	296	Sufficient for interaction with RB1
	Coiled coil	354 – 413	60	Potential
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.12
	Modified residue	304	1	Phosphothreonine Ref.12
	Modified residue	309	1	Phosphoserine Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
	Modified residue	321	1	Phosphoserine Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Natural variations
	Alternative sequence	1	1	M → MHRGGQPLKKRRGSFKM in isoform 2.		VSP_020509
	Alternative sequence	54 – 88	35	Missing in isoform 3.		VSP_020510
Experimental info
	Sequence conflict	436	1	E → V in AAH43444. Ref.5
	Sequence conflict	508	1	S → R in AAV41873. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Lin9-L) (Long form) [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 1107865E6FF33037	FASTA	542	61,946
	10 20 30 40 50 60 MAELDQLPDE SSSAKALVSL KEGSLSNTWN EKYSSLQKTP VWKGRNTSSA VEMPFRNSKR 70 80 90 100 110 120 SRLFSDEDDR QINTRSPKRN QRVAMVPQKF TATMSTPDKK ASQKIGFRLR NLLKLPKAHK 130 140 150 160 170 180 WCIYEWFYSN IDKPLFEGDN DFCVCLKESF PNLKTRKLTR VEWGKIRRLM GKPRRCSSAF 190 200 210 220 230 240 FEEERSALKQ KRQKIRLLQQ RKVADVSQFK DLPDEIPLPL VIGTKVTARL RGVHDGLFTG 250 260 270 280 290 300 QIDAVDTLNA TYRVTFDRTG LGTHTIPDYE VLSNEPHETM PIAAFGQKQR PSRFFMTPPR 310 320 330 340 350 360 LHYTPPLQSP IIDNDPLLGQ SPWRSKISGS DTETLGGFPV EFLIQVTRLS KILMIKKEHI 370 380 390 400 410 420 KKLREMNTEA EKLKSYSMPI SIEFQRRYAT IVLELEQLNK DLNKVLHKVQ QYCYELAPDQ 430 440 450 460 470 480 GLQPADQPTD MRRRCEEEAQ EIVRHANSST GQPCVENENL TDLISRLTAI LLQIKCLAEG 490 500 510 520 530 540 GDLNSFEFKS LTDSLNDIKS TIDASNISCF QNNVEIHVAH IQSGLSQMGN LHAFAANNTN RD
	Isoform 2 [UniParc]. Checksum: 90ECE86266AF02CE	FASTA	558	63,811
	10 20 30 40 50 60 MHRGGQPLKK RRGSFKMAEL DQLPDESSSA KALVSLKEGS LSNTWNEKYS SLQKTPVWKG 70 80 90 100 110 120 RNTSSAVEMP FRNSKRSRLF SDEDDRQINT RSPKRNQRVA MVPQKFTATM STPDKKASQK 130 140 150 160 170 180 IGFRLRNLLK LPKAHKWCIY EWFYSNIDKP LFEGDNDFCV CLKESFPNLK TRKLTRVEWG 190 200 210 220 230 240 KIRRLMGKPR RCSSAFFEEE RSALKQKRQK IRLLQQRKVA DVSQFKDLPD EIPLPLVIGT 250 260 270 280 290 300 KVTARLRGVH DGLFTGQIDA VDTLNATYRV TFDRTGLGTH TIPDYEVLSN EPHETMPIAA 310 320 330 340 350 360 FGQKQRPSRF FMTPPRLHYT PPLQSPIIDN DPLLGQSPWR SKISGSDTET LGGFPVEFLI 370 380 390 400 410 420 QVTRLSKILM IKKEHIKKLR EMNTEAEKLK SYSMPISIEF QRRYATIVLE LEQLNKDLNK 430 440 450 460 470 480 VLHKVQQYCY ELAPDQGLQP ADQPTDMRRR CEEEAQEIVR HANSSTGQPC VENENLTDLI 490 500 510 520 530 540 SRLTAILLQI KCLAEGGDLN SFEFKSLTDS LNDIKSTIDA SNISCFQNNV EIHVAHIQSG 550 LSQMGNLHAF AANNTNRD
	Isoform 3 (Lin9-S) (Short form) [UniParc]. Checksum: B657E9DD7A2882F0	FASTA	507	57,733
	10 20 30 40 50 60 MAELDQLPDE SSSAKALVSL KEGSLSNTWN EKYSSLQKTP VWKGRNTSSA VEMKFTATMS 70 80 90 100 110 120 TPDKKASQKI GFRLRNLLKL PKAHKWCIYE WFYSNIDKPL FEGDNDFCVC LKESFPNLKT 130 140 150 160 170 180 RKLTRVEWGK IRRLMGKPRR CSSAFFEEER SALKQKRQKI RLLQQRKVAD VSQFKDLPDE 190 200 210 220 230 240 IPLPLVIGTK VTARLRGVHD GLFTGQIDAV DTLNATYRVT FDRTGLGTHT IPDYEVLSNE 250 260 270 280 290 300 PHETMPIAAF GQKQRPSRFF MTPPRLHYTP PLQSPIIDND PLLGQSPWRS KISGSDTETL 310 320 330 340 350 360 GGFPVEFLIQ VTRLSKILMI KKEHIKKLRE MNTEAEKLKS YSMPISIEFQ RRYATIVLEL 370 380 390 400 410 420 EQLNKDLNKV LHKVQQYCYE LAPDQGLQPA DQPTDMRRRC EEEAQEIVRH ANSSTGQPCV 430 440 450 460 470 480 ENENLTDLIS RLTAILLQIK CLAEGGDLNS FEFKSLTDSL NDIKSTIDAS NISCFQNNVE 490 500 IHVAHIQSGL SQMGNLHAFA ANNTNRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inhibition of oncogenic transformation by mammalian Lin-9, a pRB-associated protein." Gagrica S., Hauser S., Kolfschoten I., Osterloh L., Agami R., Gaubatz S. EMBO J. 23:4627-4638(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Cervix carcinoma.
[2]	"A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes." Sandoval R., Xue J., Tian X., Barrett K., Pilkinton M., Ucker D.S., Raychaudhuri P., Kineman R.D., Luque R.M., Baida G., Zou X., Valli V.E., Cook J.L., Kiyokawa H., Colamonici O.R. Exp. Cell Res. 312:2465-2475(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION, SUBCELLULAR LOCATION. Tissue: Myeloma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Thymus.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Heart.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[6]	"LINC, a human complex that is related to pRB-containing complexes in invertebrates regulates the expression of G2/M genes." Schmit F., Korenjak M., Mannefeld M., Schmitt K., Franke C., von Eyss B., Gagrica S., Haenel F., Brehm A., Gaubatz S. Cell Cycle 6:1903-1913(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE DREAM COMPLEX.
[7]	"Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence." Litovchick L., Sadasivam S., Florens L., Zhu X., Swanson S.K., Velmurugan S., Chen R., Washburn M.P., Liu X.S., DeCaprio J.A. Mol. Cell 26:539-551(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE DREAM COMPLEX.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309 AND SER-321, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309 AND SER-321, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309 AND SER-321, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309 AND SER-321, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; THR-304; SER-309 AND SER-321, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY786184 mRNA. Translation: AAV41873.1. AF190323 mRNA. Translation: AAQ13710.1. AF190324 mRNA. Translation: AAQ13711.1. AK126177 mRNA. Translation: BAC86475.1. BX537869 mRNA. Translation: CAD97871.1. Different initiation. BC043444 mRNA. Translation: AAH43444.1. Different initiation. BC045625 mRNA. Translation: AAH45625.1.
IPI	IPI00445335. IPI00844013. IPI01012571.
RefSeq	NP_001257338.1. NM_001270409.1. NP_001257339.1. NM_001270410.1. NP_775106.2. NM_173083.3.
UniGene	Hs.120817.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5TKA1. 13 interactions.
MINT	MINT-4054198.
STRING	9606.ENSP00000329102.
PTM databases
PhosphoSite	Q5TKA1.
Polymorphism databases
DMDM	74708186.
Proteomic databases
PaxDb	Q5TKA1.
PRIDE	Q5TKA1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328205; ENSP00000329102; ENSG00000183814. ENST00000366801; ENSP00000355766; ENSG00000183814.
GeneID	286826.
KEGG	hsa:286826.
UCSC	uc001hqa.2. human. uc001hqb.2. human. uc001hqc.3. human.
Organism-specific databases
CTD	286826.
GeneCards	GC01M226420.
HGNC	HGNC:30830. LIN9.
HPA	HPA030241.
MIM	609375. gene.
neXtProt	NX_Q5TKA1.
PharmGKB	PA134970771.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148048.
HOGENOM	HOG000007517.
HOVERGEN	HBG057743.
InParanoid	Q5TKA1.
OrthoDB	EOG4R502K.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
ArrayExpress	Q5TKA1.
Bgee	Q5TKA1.
CleanEx	HS_LIN9.
Genevestigator	Q5TKA1.
GermOnline	ENSG00000183814. Homo sapiens.
Family and domain databases
InterPro	IPR010561. DIRP. [Graphical view]
Pfam	PF06584. DIRP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	286826.
NextBio	96300.
SOURCE	Search...

Entry information

Entry name

LIN9_HUMAN

Accession

Primary (citable) accession number: Q5TKA1
Secondary accession number(s): Q5U5L8 , Q5U7E1, Q6PI55, Q6ZTV4, Q7Z3J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.