Reviewed, UniProtKB/Swiss-Prot Q5TJF5 (DHB8_CANFA)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 8
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase 8
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 8
      Short name=17-beta-HSD 8

Gene names

Name:

HSD17B8

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Uses estradiol as its preferred substrate By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Estradiol 17-beta-dehydrogenase 8		PRO_0000054597
Regions
	Nucleotide binding	13 – 37	25	NAD By similarity
Sites
	Active site	167	1	Proton acceptor By similarity
	Binding site	154	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5TJF5-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 40AA25885DE5E197	FASTA	259	26,712
	10 20 30 40 50 60 MASPLRLRSA LALVTGAGSG IGRAVSVRLA KEGATVAACD LDRAAACETV WLLGGQGSEK 70 80 90 100 110 120 VAPGGAHTAF QADVSEAGAV RRLLEQVQAC FSRPPSVVVS CAGLTRDEFL LRMSEDDWDR 130 140 150 160 170 180 VIAVNLKGIF LVTQAAAQAL VSSGCRGSII NISSIVGKVG NVGQTNYAAS KAGVIGLTQT 190 200 210 220 230 240 AARELGRHGI RCNSVLPGFI TTPMTQKVPQ KVLDKVIGMI PMGHLGDPED VADAVTFLAS 250 EDSGYITGAS VEVTGGLYM

References

[1]

"Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species." Debenham S.L., Hart E.A., Ashurst J.L., Howe K.L., Quail M.A., Ollier W.E.R., Binns M.M. Genomics 85:48-59(2005) [PubMed: 15607421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Doberman pinscher.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ630366 Genomic DNA. Translation: CAI11433.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000000927. Canis familiaris. [Contig view]
Phylogenomic databases
HOVERGEN	Q5TJF5.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

DHB8_CANFA

Accession

Primary (citable) accession number: Q5TJF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 21, 2004
Last modified:	November 4, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)