Q5TJE4 (TPSN_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tapasin
Short name=TPN
Short name=TPSN

Alternative name(s):
TAP-binding protein

Gene names

Name:

TAPBP

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading) By similarity.
Subunit structure	Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, clareticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Domain	The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region By similarity.
Sequence similarities	Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I Inferred from electronic annotation. Source: InterPro antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Inferred from electronic annotation. Source: Compara defense response Inferred from electronic annotation. Source: Compara peptide antigen stabilization Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: Compara TAP complex Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 449	429	Tapasin		PRO_0000041838
Regions
	Topological domain	21 – 413	393	Lumenal Potential
	Transmembrane	414 – 434	21	Helical; Potential
	Topological domain	435 – 449	15	Cytoplasmic Potential
	Domain	292 – 399	108	Ig-like C1-type
	Compositional bias	242 – 245	4	Poly-Asp
Sites
	Site	428	1	May be involved in interaction with TAP By similarity
Amino acid modifications
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 91		By similarity
	Disulfide bond	115		Interchain (with C-57 in PDIA3) By similarity
	Disulfide bond	315 ↔ 382		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5TJE4 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: A6A661D28ACDA7BB	FASTA	449	47,774
	10 20 30 40 50 60 MKPLSLLLAV ASALGTAVSA GPAVIECWMV EDAGGGRLAK KPAALLLRQG PGTPPPRPDL 70 80 90 100 110 120 EPELYLKVHD PAGTLQAAVR RYPSDAPPPH CELSRFIPLP ASARWARGLT PGRSCPRALD 130 140 150 160 170 180 GAWLMASVLS PIFSLSCLLR PQSEPQPEPA LFTSATAVLT VLTYSPIAQI QLGQDALLDL 190 200 210 220 230 240 RFAYMPSISE AAASLAPGPP PFGLEWRRQH LGKGHLLLAA TPGLHEQMPA AQDGAVAFAA 250 260 270 280 290 300 WDDDDPWGPW TGNGTLWLPA VQPFQEGTYL ATVHLPYLQG QVALELSVQK PPKISLTPAP 310 320 330 340 350 360 LVWAAPGEAP PELLCLVSRF YPAKGLEVEW ELRGGPEGSF QKAEGQSWLS ALRHHSDGSV 370 380 390 400 410 420 SLSAHLQPIP VTAKHHGARY ACRVHHPTLP TLGRSAEVTL EVAGLSGPSL EDSVGLFLSA 430 440 FLLLGLIKAL GWVAASRSTS KDPKEKKAQ

References

[1]

"Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species." Debenham S.L., Hart E.A., Ashurst J.L., Howe K.L., Quail M.A., Ollier W.E.R., Binns M.M. Genomics 85:48-59(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Doberman pinscher.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ630366 Genomic DNA. Translation: CAI11444.1.
RefSeq	NP_001041566.1. NM_001048101.1.
UniGene	Cfa.46213.
3D structure databases
ProteinModelPortal	Q5TJE4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000001502; ENSCAFP00000001381; ENSCAFG00000000972.
GeneID	481740.
KEGG	cfa:481740.
Organism-specific databases
CTD	6892.
Phylogenomic databases
eggNOG	NOG41000.
GeneTree	ENSGT00530000063758.
HOGENOM	HOG000231214.
HOVERGEN	HBG005156.
InParanoid	Q5TJE4.
KO	K08058.
OMA	HGARYAC.
OrthoDB	EOG469QV7.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR008056. Tapasin. [Graphical view]
PANTHER	PTHR23411. PTHR23411. 1 hit.
Pfam	PF07654. C1-set. 1 hit. [Graphical view]
PRINTS	PR01669. TAPASIN.
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20856483.

Entry information

Entry name

TPSN_CANFA

Accession

Primary (citable) accession number: Q5TJE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program