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Protein

Protein THEMIS2

Gene

THEMIS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TNF production.1 Publication

GO - Biological processi

  • cell adhesion Source: ProtInc
  • inflammatory response Source: UniProtKB-KW
  • T cell receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein THEMIS2
Alternative name(s):
Induced by contact to basement membrane 1 protein
Short name:
Protein ICB-1
Thymocyte-expressed molecule involved in selection protein 2
Gene namesi
Name:THEMIS2
Synonyms:C1orf38, ICB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:16839. THEMIS2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi9473.
OpenTargetsiENSG00000130775.
PharmGKBiPA128394550.

Polymorphism and mutation databases

BioMutaiTHEMIS2.
DMDMi73920021.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000841431 – 643Protein THEMIS2Add BLAST643

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei593PhosphothreonineCombined sources1
Modified residuei632PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation at Tyr-632 is induced by LPS.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5TEJ8.
MaxQBiQ5TEJ8.
PaxDbiQ5TEJ8.
PeptideAtlasiQ5TEJ8.
PRIDEiQ5TEJ8.

PTM databases

iPTMnetiQ5TEJ8.
PhosphoSitePlusiQ5TEJ8.

Expressioni

Tissue specificityi

Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.1 Publication

Inductioni

By contact to a reconstituted basement membrane.

Gene expression databases

BgeeiENSG00000130775.
CleanExiHS_C1orf38.
ExpressionAtlasiQ5TEJ8. baseline and differential.
GenevisibleiQ5TEJ8. HS.

Organism-specific databases

HPAiHPA031096.
HPA031097.

Interactioni

Subunit structurei

When phosphorylated, interacts with LYN. Interacts with VAV1 and GRB2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NOTCH2NLQ7Z3S93EBI-2806032,EBI-945833

Protein-protein interaction databases

BioGridi114858. 3 interactors.
IntActiQ5TEJ8. 3 interactors.
STRINGi9606.ENSP00000363031.

Structurei

3D structure databases

ProteinModelPortaliQ5TEJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 238CABIT 1Add BLAST238
Regioni239 – 514CABIT 2Add BLAST276

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi404 – 409Poly-Glu6
Compositional biasi626 – 637Asp/Glu-richAdd BLAST12

Sequence similaritiesi

Belongs to the themis family.Curated

Phylogenomic databases

eggNOGiENOG410IF5X. Eukaryota.
ENOG4110SKT. LUCA.
GeneTreeiENSGT00530000063770.
InParanoidiQ5TEJ8.
OMAiFMSTHRI.
OrthoDBiEOG091G02V6.
PhylomeDBiQ5TEJ8.
TreeFamiTF333479.

Family and domain databases

InterProiIPR025946. CABIT_dom.
[Graphical view]
PfamiPF12736. CABIT. 2 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5TEJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPVPLQDFV RALDPASLPR VLRVCSGVYF EGSIYEISGN ECCLSTGDLI
60 70 80 90 100
KVTQVRLQKV VCENPKTSQT MELAPNFQGY FTPLNTPQSY ETLEELVSAT
110 120 130 140 150
TQSSKQLPTC FMSTHRIVTE GRVVTEDQLL MLEAVVMHLG IRSARCVLGM
160 170 180 190 200
EGQQVILHLP LSQKGPFWTW EPSAPRTLLQ VLQDPALKDL VLTCPTLPWH
210 220 230 240 250
SLILRPQYEI QAIMHMRRTI VKIPSTLEVD VEDVTASSRH VHFIKPLLLS
260 270 280 290 300
EVLAWEGPFP LSMEILEVPE GRPIFLSPWV GSLQKGQRLC VYGLASPPWR
310 320 330 340 350
VLASSKGRKV PRHFLVSGGY QGKLRRRPRE FPTAYDLLGA FQPGRPLRVV
360 370 380 390 400
ATKDCEGERE ENPEFTSLAV GDRLEVLGPG QAHGAQGSDV DVLVCQRLSD
410 420 430 440 450
QAGEDEEEEC KEEAESPERV LLPFHFPGSF VEEMSDSRRY SLADLTAQFS
460 470 480 490 500
LPCEVKVVAK DTSHPTDPLT SFLGLRLEEK ITEPFLVVSL DSEPGMCFEI
510 520 530 540 550
PPRWLDLTVV KAKGQPDLPE GSLPIATVEE LTDTFYYRLR KLPACEIQAP
560 570 580 590 600
PPRPPKNQGL SKQRRHSSEG GVKSSQVLGL QQHARLPKPK AKTLPEFIKD
610 620 630 640
GSSTYSKIPA HRKGHRPAKP QRQDLDDDEH DYEEILEQFQ KTI
Length:643
Mass (Da):72,049
Last modified:December 21, 2004 - v1
Checksum:i1D5FA24F88D2F8EA
GO
Isoform 2 (identifier: Q5TEJ8-2) [UniParc]FASTAAdd to basket
Also known as: ICB1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     216-260: MRRTIVKIPS...EVLAWEGPFP → IFSSLRIAAT...SCPQEGPQAR
     261-643: Missing.

Note: No experimental confirmation available.
Show »
Length:260
Mass (Da):29,118
Checksum:iB4B5C4BFF16682D7
GO
Isoform 3 (identifier: Q5TEJ8-3) [UniParc]FASTAAdd to basket
Also known as: ICB1-gamma

The sequence of this isoform differs from the canonical sequence as follows:
     252-396: Missing.

Show »
Length:498
Mass (Da):56,130
Checksum:iF87DC9EA1DF4901D
GO
Isoform 4 (identifier: Q5TEJ8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-123: GYFTPLNTPQ...THRIVTEGRV → VFSSLRIAAT...SCPQEGPQAR
     124-643: Missing.

Show »
Length:123
Mass (Da):13,628
Checksum:iFA37B14081402515
GO
Isoform 5 (identifier: Q5TEJ8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-396: Missing.

Note: No experimental confirmation available.
Show »
Length:514
Mass (Da):57,943
Checksum:iC5FA6EECF503BD9C
GO
Isoform 6 (identifier: Q5TEJ8-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-381: Missing.

Note: No experimental confirmation available.
Show »
Length:447
Mass (Da):50,028
Checksum:i5E02ACFCD1435979
GO

Sequence cautioni

The sequence AAC16284 differs from that shown. Reason: Frameshift at positions 416, 469, 494, 530 and 532.Curated
The sequence BAA96464 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB33313 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG64201 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI21769 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI21771 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI21773 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti451L → M in AAC16284 (Ref. 6) Curated1
Sequence conflicti466T → N in AAC16284 (Ref. 6) Curated1
Sequence conflicti504W → R in AAC16284 (Ref. 6) Curated1
Sequence conflicti538R → C in AAC16284 (Ref. 6) Curated1
Sequence conflicti584A → V in AAC16284 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051060431V → L.Corresponds to variant rs35995543dbSNPEnsembl.1
Natural variantiVAR_051061511K → E.2 PublicationsCorresponds to variant rs3766400dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01532879 – 123GYFTP…TEGRV → VFSSLRIAATRSAAQTQGED LARVHQGWLQYVQQDSCPQE GPQAR in isoform 4. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_015329124 – 643Missing in isoform 4. 1 PublicationAdd BLAST520
Alternative sequenceiVSP_037966186 – 381Missing in isoform 6. 1 PublicationAdd BLAST196
Alternative sequenceiVSP_015326216 – 260MRRTI…EGPFP → IFSSLRIAATRSAAQTQGED LARVHQGWLQYVQQDSCPQE GPQAR in isoform 2. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_015330252 – 396Missing in isoform 3. 1 PublicationAdd BLAST145
Alternative sequenceiVSP_015327261 – 643Missing in isoform 2. 2 PublicationsAdd BLAST383
Alternative sequenceiVSP_037967268 – 396Missing in isoform 5. 1 PublicationAdd BLAST129

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR749321 mRNA. Translation: CAH18176.1.
AK303090 mRNA. Translation: BAG64201.1. Different initiation.
AK303141 mRNA. Translation: BAG64245.1.
AL109927 Genomic DNA. Translation: CAI21769.1. Sequence problems.
AL109927 Genomic DNA. Translation: CAI21770.1.
AL109927 Genomic DNA. Translation: CAI21771.1. Sequence problems.
AL109927 Genomic DNA. Translation: CAI21772.1.
AL109927 Genomic DNA. Translation: CAI21773.1. Sequence problems.
BC031655 mRNA. Translation: AAH31655.2.
BC132692 mRNA. Translation: AAI32693.1.
BC133049 mRNA. Translation: AAI33050.1.
AF044895 mRNA. Translation: AAC16284.1. Frameshift.
AB050854 mRNA. Translation: BAB33313.1. Different initiation.
AB035482 mRNA. Translation: BAA96464.1. Different initiation.
CCDSiCCDS30653.1. [Q5TEJ8-2]
CCDS30654.1. [Q5TEJ8-4]
CCDS41290.1. [Q5TEJ8-1]
CCDS65461.1. [Q5TEJ8-5]
RefSeqiNP_001034566.1. NM_001039477.2. [Q5TEJ8-4]
NP_001099026.1. NM_001105556.2. [Q5TEJ8-1]
NP_001273042.1. NM_001286113.1. [Q5TEJ8-5]
NP_001273044.1. NM_001286115.1. [Q5TEJ8-6]
NP_004839.2. NM_004848.3. [Q5TEJ8-2]
XP_006711113.1. XM_006711050.1. [Q5TEJ8-3]
UniGeneiHs.10649.

Genome annotation databases

EnsembliENST00000328928; ENSP00000329862; ENSG00000130775. [Q5TEJ8-5]
ENST00000373921; ENSP00000363031; ENSG00000130775. [Q5TEJ8-1]
ENST00000373925; ENSP00000363035; ENSG00000130775. [Q5TEJ8-2]
ENST00000373927; ENSP00000363037; ENSG00000130775. [Q5TEJ8-4]
GeneIDi9473.
KEGGihsa:9473.
UCSCiuc001boz.5. human. [Q5TEJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR749321 mRNA. Translation: CAH18176.1.
AK303090 mRNA. Translation: BAG64201.1. Different initiation.
AK303141 mRNA. Translation: BAG64245.1.
AL109927 Genomic DNA. Translation: CAI21769.1. Sequence problems.
AL109927 Genomic DNA. Translation: CAI21770.1.
AL109927 Genomic DNA. Translation: CAI21771.1. Sequence problems.
AL109927 Genomic DNA. Translation: CAI21772.1.
AL109927 Genomic DNA. Translation: CAI21773.1. Sequence problems.
BC031655 mRNA. Translation: AAH31655.2.
BC132692 mRNA. Translation: AAI32693.1.
BC133049 mRNA. Translation: AAI33050.1.
AF044895 mRNA. Translation: AAC16284.1. Frameshift.
AB050854 mRNA. Translation: BAB33313.1. Different initiation.
AB035482 mRNA. Translation: BAA96464.1. Different initiation.
CCDSiCCDS30653.1. [Q5TEJ8-2]
CCDS30654.1. [Q5TEJ8-4]
CCDS41290.1. [Q5TEJ8-1]
CCDS65461.1. [Q5TEJ8-5]
RefSeqiNP_001034566.1. NM_001039477.2. [Q5TEJ8-4]
NP_001099026.1. NM_001105556.2. [Q5TEJ8-1]
NP_001273042.1. NM_001286113.1. [Q5TEJ8-5]
NP_001273044.1. NM_001286115.1. [Q5TEJ8-6]
NP_004839.2. NM_004848.3. [Q5TEJ8-2]
XP_006711113.1. XM_006711050.1. [Q5TEJ8-3]
UniGeneiHs.10649.

3D structure databases

ProteinModelPortaliQ5TEJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114858. 3 interactors.
IntActiQ5TEJ8. 3 interactors.
STRINGi9606.ENSP00000363031.

PTM databases

iPTMnetiQ5TEJ8.
PhosphoSitePlusiQ5TEJ8.

Polymorphism and mutation databases

BioMutaiTHEMIS2.
DMDMi73920021.

Proteomic databases

EPDiQ5TEJ8.
MaxQBiQ5TEJ8.
PaxDbiQ5TEJ8.
PeptideAtlasiQ5TEJ8.
PRIDEiQ5TEJ8.

Protocols and materials databases

DNASUi9473.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328928; ENSP00000329862; ENSG00000130775. [Q5TEJ8-5]
ENST00000373921; ENSP00000363031; ENSG00000130775. [Q5TEJ8-1]
ENST00000373925; ENSP00000363035; ENSG00000130775. [Q5TEJ8-2]
ENST00000373927; ENSP00000363037; ENSG00000130775. [Q5TEJ8-4]
GeneIDi9473.
KEGGihsa:9473.
UCSCiuc001boz.5. human. [Q5TEJ8-1]

Organism-specific databases

CTDi9473.
DisGeNETi9473.
GeneCardsiTHEMIS2.
HGNCiHGNC:16839. THEMIS2.
HPAiHPA031096.
HPA031097.
neXtProtiNX_Q5TEJ8.
OpenTargetsiENSG00000130775.
PharmGKBiPA128394550.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF5X. Eukaryota.
ENOG4110SKT. LUCA.
GeneTreeiENSGT00530000063770.
InParanoidiQ5TEJ8.
OMAiFMSTHRI.
OrthoDBiEOG091G02V6.
PhylomeDBiQ5TEJ8.
TreeFamiTF333479.

Miscellaneous databases

GenomeRNAii9473.
PROiQ5TEJ8.

Gene expression databases

BgeeiENSG00000130775.
CleanExiHS_C1orf38.
ExpressionAtlasiQ5TEJ8. baseline and differential.
GenevisibleiQ5TEJ8. HS.

Family and domain databases

InterProiIPR025946. CABIT_dom.
[Graphical view]
PfamiPF12736. CABIT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHMS2_HUMAN
AccessioniPrimary (citable) accession number: Q5TEJ8
Secondary accession number(s): A2RTZ3
, B4DZT9, B4DZY3, O60560, Q5TEJ1, Q5TEJ9, Q5TEK1, Q68DP4, Q9BYB6, Q9NS90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.