Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Four and a half LIM domains protein 5

Gene

FHL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 3225C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. transcription coactivator activity Source: Ensembl
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 5
Short name:
FHL-5
Alternative name(s):
Activator of cAMP-responsive element modulator in testis
Short name:
Activator of CREM in testis
Gene namesi
Name:FHL5
Synonyms:ACT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:17371. FHL5.

Subcellular locationi

  1. Nucleus 1 Publication

  2. Note: Nuclei of round and elongated spermatids.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134971720.

Polymorphism and mutation databases

BioMutaiFHL5.
DMDMi68565455.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Four and a half LIM domains protein 5PRO_0000075742Add
BLAST

Proteomic databases

PaxDbiQ5TD97.
PRIDEiQ5TD97.

PTM databases

PhosphoSiteiQ5TD97.

Expressioni

Tissue specificityi

Testis-specific (at protein level).1 Publication

Gene expression databases

BgeeiQ5TD97.
CleanExiHS_FHL5.
ExpressionAtlasiQ5TD97. baseline and differential.
GenevestigatoriQ5TD97.

Organism-specific databases

HPAiHPA045151.

Interactioni

Subunit structurei

Interacts with CREM via the third LIM domain.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hoxa1P090223EBI-750641,EBI-3957603From a different organism.

Protein-protein interaction databases

BioGridi114846. 18 interactions.
IntActiQ5TD97. 5 interactions.
MINTiMINT-2812584.
STRINGi9606.ENSP00000326022.

Structurei

Secondary structure

1
284
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni223 – 2253Combined sources
Turni231 – 2344Combined sources
Beta strandi237 – 2404Combined sources
Beta strandi243 – 2464Combined sources
Helixi247 – 2493Combined sources
Turni253 – 2553Combined sources
Beta strandi260 – 2623Combined sources
Beta strandi264 – 2674Combined sources
Beta strandi270 – 2734Combined sources
Turni274 – 2774Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X68NMR-A222-284[»]
ProteinModelPortaliQ5TD97.
SMRiQ5TD97. Positions 3-284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5TD97.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 10062LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini101 – 16060LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini161 – 22060LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini223 – 28361LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 3225C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiQ5TD97.
OMAiYCVSCYD.
OrthoDBiEOG7P8P7M.
PhylomeDBiQ5TD97.
TreeFamiTF314113.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5TD97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAHFYCQY CTASLLGKKY VLKDDSPYCV TCYDRVFSNY CEECKKPIES
60 70 80 90 100
DSKDLCYKDR HWHEGCFKCT KCNHSLVEKP FAAKDERLLC TECYSNECSS
110 120 130 140 150
KCFHCKRTIM PGSRKMEFKG NYWHETCFVC ENCRQPIGTK PLISKESGNY
160 170 180 190 200
CVPCFEKEFA HYCNFCKKVI TSGGITFCDQ LWHKECFLCS GCRKDLCEEQ
210 220 230 240 250
FMSRDDYPFC VDCYNHLYAN KCVACSKPIS GLTGAKFICF QDSQWHSECF
260 270 280
NCGKCSVSLV GKGFLTQNKE IFCQKCGSGM DTDI
Length:284
Mass (Da):32,720
Last modified:December 21, 2004 - v1
Checksum:i82C03B1EAA147C11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551L → F in AAF85978 (PubMed:11327716).Curated
Sequence conflicti191 – 1911G → D in AAH21723 (PubMed:15489334).Curated
Sequence conflicti191 – 1911G → D in AAH29490 (PubMed:15489334).Curated
Sequence conflicti254 – 2541K → R in AAF85978 (PubMed:11327716).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti35 – 351R → H.
Corresponds to variant rs35157931 [ dbSNP | Ensembl ].
VAR_056160
Natural varianti204 – 2041R → G.
Corresponds to variant rs2273621 [ dbSNP | Ensembl ].
VAR_056161
Natural varianti211 – 2111V → M.3 Publications
Corresponds to variant rs2252816 [ dbSNP | Ensembl ].
VAR_022824
Natural varianti243 – 2431S → R.
Corresponds to variant rs9373985 [ dbSNP | Ensembl ].
VAR_056162

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF278541 mRNA. Translation: AAF85978.1.
AK057611 mRNA. Translation: BAG51940.1.
AK223539 mRNA. Translation: BAD97259.1.
AK314608 mRNA. Translation: BAG37178.1.
AL132776 Genomic DNA. Translation: CAB86657.1.
AL132776 Genomic DNA. Translation: CAI20400.1.
CH471051 Genomic DNA. Translation: EAW48503.1.
CH471051 Genomic DNA. Translation: EAW48504.1.
CH471051 Genomic DNA. Translation: EAW48505.1.
BC021723 mRNA. Translation: AAH21723.1.
BC029490 mRNA. Translation: AAH29490.1.
CCDSiCCDS5035.1.
PIRiJC7686.
RefSeqiNP_001164278.1. NM_001170807.1.
NP_065228.4. NM_020482.4.
XP_006715676.1. XM_006715613.2.
UniGeneiHs.632608.

Genome annotation databases

EnsembliENST00000326771; ENSP00000326022; ENSG00000112214.
ENST00000541107; ENSP00000442357; ENSG00000112214.
GeneIDi9457.
KEGGihsa:9457.
UCSCiuc003pos.2. human.

Polymorphism and mutation databases

BioMutaiFHL5.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF278541 mRNA. Translation: AAF85978.1.
AK057611 mRNA. Translation: BAG51940.1.
AK223539 mRNA. Translation: BAD97259.1.
AK314608 mRNA. Translation: BAG37178.1.
AL132776 Genomic DNA. Translation: CAB86657.1.
AL132776 Genomic DNA. Translation: CAI20400.1.
CH471051 Genomic DNA. Translation: EAW48503.1.
CH471051 Genomic DNA. Translation: EAW48504.1.
CH471051 Genomic DNA. Translation: EAW48505.1.
BC021723 mRNA. Translation: AAH21723.1.
BC029490 mRNA. Translation: AAH29490.1.
CCDSiCCDS5035.1.
PIRiJC7686.
RefSeqiNP_001164278.1. NM_001170807.1.
NP_065228.4. NM_020482.4.
XP_006715676.1. XM_006715613.2.
UniGeneiHs.632608.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X68NMR-A222-284[»]
ProteinModelPortaliQ5TD97.
SMRiQ5TD97. Positions 3-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114846. 18 interactions.
IntActiQ5TD97. 5 interactions.
MINTiMINT-2812584.
STRINGi9606.ENSP00000326022.

PTM databases

PhosphoSiteiQ5TD97.

Polymorphism and mutation databases

BioMutaiFHL5.
DMDMi68565455.

Proteomic databases

PaxDbiQ5TD97.
PRIDEiQ5TD97.

Protocols and materials databases

DNASUi9457.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326771; ENSP00000326022; ENSG00000112214.
ENST00000541107; ENSP00000442357; ENSG00000112214.
GeneIDi9457.
KEGGihsa:9457.
UCSCiuc003pos.2. human.

Organism-specific databases

CTDi9457.
GeneCardsiGC06P097056.
H-InvDBHIX0006080.
HGNCiHGNC:17371. FHL5.
HPAiHPA045151.
MIMi605126. gene.
neXtProtiNX_Q5TD97.
PharmGKBiPA134971720.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiQ5TD97.
OMAiYCVSCYD.
OrthoDBiEOG7P8P7M.
PhylomeDBiQ5TD97.
TreeFamiTF314113.

Miscellaneous databases

EvolutionaryTraceiQ5TD97.
GeneWikiiFHL5.
GenomeRNAii9457.
NextBioi35438.
PROiQ5TD97.
SOURCEiSearch...

Gene expression databases

BgeeiQ5TD97.
CleanExiHS_FHL5.
ExpressionAtlasiQ5TD97. baseline and differential.
GenevestigatoriQ5TD97.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-211, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-211.
    Tissue: Trachea.
  3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-211.
    Tissue: Testis.
  7. "Solution structures of the C-terminal LIM domain of human FHL5 protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 222-284 IN COMPLEX WITH ZINC IONS.

Entry informationi

Entry nameiFHL5_HUMAN
AccessioniPrimary (citable) accession number: Q5TD97
Secondary accession number(s): B2RBD1
, E1P526, Q5TD98, Q8WW21, Q9NQU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: December 21, 2004
Last modified: April 29, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.