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Protein

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3

Gene

MAGI3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.1 Publication

Miscellaneous

MAGI3 PDZ domains are used to design peptide ligands that bind and inhibit PDZ domains.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi121 – 128ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • guanylate kinase activity Source: UniProtKB
  • protein-containing complex scaffold activity Source: InterPro

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • regulation of JNK cascade Source: InterPro
  • viral process Source: UniProtKB-KW

Keywordsi

Biological processHost-virus interaction
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ5TCQ9

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
Alternative name(s):
Membrane-associated guanylate kinase inverted 3
Short name:
MAGI-3
Gene namesi
Name:MAGI3
Synonyms:KIAA1634
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000081026.18
HGNCiHGNC:29647 MAGI3
MIMi615943 gene
neXtProtiNX_Q5TCQ9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi260425
OpenTargetsiENSG00000081026
PharmGKBiPA142671485

Chemistry databases

ChEMBLiCHEMBL5212

Polymorphism and mutation databases

BioMutaiMAGI3
DMDMi190359882

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003414071 – 1481Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3Add BLAST1481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei234PhosphoserineBy similarity1
Modified residuei595PhosphoserineCombined sources1
Modified residuei699PhosphoserineBy similarity1
Modified residuei832PhosphoserineCombined sources1
Modified residuei915PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5TCQ9
MaxQBiQ5TCQ9
PaxDbiQ5TCQ9
PeptideAtlasiQ5TCQ9
PRIDEiQ5TCQ9

PTM databases

iPTMnetiQ5TCQ9
PhosphoSitePlusiQ5TCQ9

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000081026
CleanExiHS_MAGI3
ExpressionAtlasiQ5TCQ9 baseline and differential
GenevisibleiQ5TCQ9 HS

Organism-specific databases

HPAiHPA007923
HPA008444

Interactioni

Subunit structurei

Interacts with ADRB1, FZD4, FZD7, PTPRB, TGFA and VANGL2. Interacts with unidentified tyrosine phosphorylated proteins (By similarity). Interacts with ADGRB1, LPAR2/EDG4, GRIN2B and PTEN. In case of infection, interacts with HTLV TAX1 protein, possibly affecting the transformation ability of TAX1. Does not interact with HTLV TAX2 or TAX3 proteins. In case of infection, interacts with HPV type 16 and HPV type 18 E6 protein. Interacts with DLL1 (By similarity).By similarity7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi129277, 21 interactors
IntActiQ5TCQ9, 9 interactors
MINTiQ5TCQ9
STRINGi9606.ENSP00000304604

Chemistry databases

BindingDBiQ5TCQ9

Structurei

Secondary structure

11481
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi576 – 582Combined sources7
Beta strandi587 – 595Combined sources9
Beta strandi598 – 605Combined sources8
Helixi607 – 609Combined sources3
Beta strandi618 – 622Combined sources5
Helixi632 – 641Combined sources10
Beta strandi647 – 656Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SOEX-ray1.60A576-666[»]
ProteinModelPortaliQ5TCQ9
SMRiQ5TCQ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 106PDZ 1PROSITE-ProRule annotationAdd BLAST89
Domaini114 – 288Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST175
Domaini293 – 326WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini339 – 372WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini410 – 492PDZ 2PROSITE-ProRule annotationAdd BLAST83
Domaini578 – 654PDZ 3PROSITE-ProRule annotationAdd BLAST77
Domaini726 – 808PDZ 4PROSITE-ProRule annotationAdd BLAST83
Domaini851 – 938PDZ 5PROSITE-ProRule annotationAdd BLAST88
Domaini1021 – 1103PDZ 6PROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 106Interaction with ADRB1 and TGFABy similarityAdd BLAST89
Regioni410 – 492Interaction with PTEN1 PublicationAdd BLAST83
Regioni726 – 808Interaction with ADGRB11 PublicationAdd BLAST83
Regioni851 – 938Interaction with LPAR2 and GRIN2B2 PublicationsAdd BLAST88

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 9Poly-Lys4
Compositional biasi238 – 243Poly-Glu6

Sequence similaritiesi

Belongs to the MAGUK family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0707 Eukaryota
KOG3209 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00650000092997
HOVERGENiHBG007091
InParanoidiQ5TCQ9
KOiK06112
OMAiDHGDWDI
OrthoDBiEOG091G0NSY
PhylomeDBiQ5TCQ9
TreeFamiTF316816

Family and domain databases

CDDicd00201 WW, 2 hits
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR020590 Guanylate_kinase_CS
IPR030035 MAGI3
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001202 WW_dom
IPR036020 WW_dom_sf
PANTHERiPTHR10316:SF10 PTHR10316:SF10, 2 hits
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF00595 PDZ, 4 hits
PF00397 WW, 2 hits
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00228 PDZ, 6 hits
SM00456 WW, 2 hits
SUPFAMiSSF50156 SSF50156, 6 hits
SSF51045 SSF51045, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
PS50106 PDZ, 6 hits
PS01159 WW_DOMAIN_1, 1 hit
PS50020 WW_DOMAIN_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5TCQ9-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKTLKKKKH WLSKVQECAV SWAGPPGDFG AEIRGGAERG EFPYLGRLRE
60 70 80 90 100
EPGGGTCCVV SGKAPSPGDV LLEVNGTPVS GLTNRDTLAV IRHFREPIRL
110 120 130 140 150
KTVKPGKVIN KDLRHYLSLQ FQKGSIDHKL QQVIRDNLYL RTIPCTTRAP
160 170 180 190 200
RDGEVPGVDY NFISVEQFKA LEESGALLES GTYDGNFYGT PKPPAEPSPF
210 220 230 240 250
QPDPVDQVLF DNEFDAESQR KRTTSVSKME RMDSSLPEEE EDEDKEAING
260 270 280 290 300
SGNAENRERH SESSDWMKTV PSYNQTNSSM DFRNYMMRDE TLEPLPKNWE
310 320 330 340 350
MAYTDTGMIY FIDHNTKTTT WLDPRLCKKA KAPEDCEDGE LPYGWEKIED
360 370 380 390 400
PQYGTYYVDH LNQKTQFENP VEEAKRKKQL GQVEIGSSKP DMEKSHFTRD
410 420 430 440 450
PSQLKGVLVR ASLKKSTMGF GFTIIGGDRP DEFLQVKNVL KDGPAAQDGK
460 470 480 490 500
IAPGDVIVDI NGNCVLGHTH ADVVQMFQLV PVNQYVNLTL CRGYPLPDDS
510 520 530 540 550
EDPVVDIVAA TPVINGQSLT KGETCMNPQD FKPGAMVLEQ NGKSGHTLTG
560 570 580 590 600
DGLNGPSDAS EQRVSMASSG SSQPELVTIP LIKGPKGFGF AIADSPTGQK
610 620 630 640 650
VKMILDSQWC QGLQKGDIIK EIYHQNVQNL THLQVVEVLK QFPVGADVPL
660 670 680 690 700
LILRGGPPSP TKTAKMKTDK KENAGSLEAI NEPIPQPMPF PPSIIRSGSP
710 720 730 740 750
KLDPSEVYLK SKTLYEDKPP NTKDLDVFLR KQESGFGFRV LGGDGPDQSI
760 770 780 790 800
YIGAIIPLGA AEKDGRLRAA DELMCIDGIP VKGKSHKQVL DLMTTAARNG
810 820 830 840 850
HVLLTVRRKI FYGEKQPEDD SSQAFISTQN GSPRLNRAEV PARPAPQEPY
860 870 880 890 900
DVVLQRKENE GFGFVILTSK NKPPPGVIPH KIGRVIEGSP ADRCGKLKVG
910 920 930 940 950
DHISAVNGQS IVELSHDNIV QLIKDAGVTV TLTVIAEEEH HGPPSGTNSA
960 970 980 990 1000
RQSPALQHRP MGQSQANHIP GDRSALEGEI GKDVSTSYRH SWSDHKHLAQ
1010 1020 1030 1040 1050
PDTAVISVVG SRHNQNLGCY PVELERGPRG FGFSLRGGKE YNMGLFILRL
1060 1070 1080 1090 1100
AEDGPAIKDG RIHVGDQIVE INGEPTQGIT HTRAIELIQA GGNKVLLLLR
1110 1120 1130 1140 1150
PGTGLIPDHG DWDINNPSSS NVIYDEQSPL PPSSHFASIF EESHVPVIEE
1160 1170 1180 1190 1200
SLRVQICEKA EELKDIVPEK KSTLNENQPE IKHQSLLQKN VSKRDPPSSH
1210 1220 1230 1240 1250
GHSNKKNLLK VENGVTRRGR SVSPKKPASQ HSEEHLDKIP SPLKNNPKRR
1260 1270 1280 1290 1300
PRDQSLSPSK GENKSCQVST RAGSGQDQCR KSRGRSASPK KQQKIEGSKA
1310 1320 1330 1340 1350
PSNAEAKLLE GKSRRIAGYT GSNAEQIPDG KEKSDVIRKD AKQNQLEKSR
1360 1370 1380 1390 1400
TRSPEKKIKR MVEKSLPSKM TNKTTSKEVS ENEKGKKVTT GETSSSNDKI
1410 1420 1430 1440 1450
GENVQLSEKR LKQEPEEKVV SNKTEDHKGK ELEAADKNKE TGRFKPESSS
1460 1470 1480
PVKKTLITPG PWKVPSGNKV TGTIGMAEKR Q
Note: No experimental confirmation available.Curated
Length:1,481
Mass (Da):162,949
Last modified:March 28, 2018 - v3
Checksum:i9C2B604D9B1B1B97
GO
Isoform 4 (identifier: Q5TCQ9-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-359: D → DFTLVAQAGVQWHDLGSLQPPPPGFN

Note: No experimental confirmation available.Curated
Show »
Length:1,506
Mass (Da):165,608
Checksum:i0EA98F09F9AB109F
GO
Isoform 2 (identifier: Q5TCQ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-359: D → DFTLVAQAGVQWHDLGSLQPPPPGFN
     1111-1125: DWDINNPSSSNVIYD → LAPSGLCSYVKPEQH
     1126-1481: Missing.

Show »
Length:1,150
Mass (Da):125,998
Checksum:i14DED25795278E3C
GO
Isoform 3 (identifier: Q5TCQ9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1111-1125: DWDINNPSSSNVIYD → LAPSGLCSYVKPEQH
     1126-1481: Missing.

Show »
Length:1,125
Mass (Da):123,339
Checksum:iFA2340EA152503E9
GO

Sequence cautioni

The sequence BAB15479 differs from that shown. Reason: Frameshift at position 1348.Curated
The sequence BAB15479 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9K → R in AAG43837 (PubMed:15652357).Curated1
Sequence conflicti59V → I in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti66 – 68SPG → NPS in AAG24545 (PubMed:10748157).Curated3
Sequence conflicti246E → G in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti257R → G in AAG43837 (PubMed:15652357).Curated1
Sequence conflicti466L → F in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti548L → S in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti616G → E in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti660P → T in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti776I → V in AAG43837 (PubMed:15652357).Curated1
Sequence conflicti798R → P in AAG43837 (PubMed:15652357).Curated1
Sequence conflicti917D → A in AAG24545 (PubMed:10748157).Curated1
Sequence conflicti993S → P in AAG43837 (PubMed:15652357).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059502359D → DFTLVAQAGVQWHDLGSLQP PPPGFN in isoform 4 and isoform 2. 3 Publications1
Alternative sequenceiVSP_0595031111 – 1125DWDIN…NVIYD → LAPSGLCSYVKPEQH in isoform 3 and isoform 2. 4 PublicationsAdd BLAST15
Alternative sequenceiVSP_0595041126 – 1481Missing in isoform 3 and isoform 2. 4 PublicationsAdd BLAST356

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257238 mRNA Translation: AAG24545.1
AF213259 mRNA Translation: AAG43837.1
AL133517 Genomic DNA No translation available.
AL365225 Genomic DNA No translation available.
AL389921 Genomic DNA No translation available.
AL390759 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56561.1
CH471122 Genomic DNA Translation: EAW56559.1
CH471122 Genomic DNA Translation: EAW56560.1
CH471122 Genomic DNA Translation: EAW56563.1
CH471122 Genomic DNA Translation: EAW56562.1
BC130409 mRNA Translation: AAI30410.1
AB046854 mRNA Translation: BAB13460.1
AK026417 mRNA Translation: BAB15479.1 Sequence problems.
CCDSiCCDS44196.1 [Q5TCQ9-4]
CCDS860.1 [Q5TCQ9-3]
RefSeqiNP_001136254.1, NM_001142782.1 [Q5TCQ9-4]
NP_690864.2, NM_152900.2 [Q5TCQ9-3]
XP_005270794.1, XM_005270737.3 [Q5TCQ9-3]
UniGeneiHs.486189

Genome annotation databases

EnsembliENST00000307546; ENSP00000304604; ENSG00000081026 [Q5TCQ9-4]
ENST00000369611; ENSP00000358624; ENSG00000081026 [Q5TCQ9-3]
ENST00000369615; ENSP00000358628; ENSG00000081026 [Q5TCQ9-3]
ENST00000369617; ENSP00000358630; ENSG00000081026 [Q5TCQ9-2]
GeneIDi260425
KEGGihsa:260425
UCSCiuc001edh.4 human [Q5TCQ9-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMAGI3_HUMAN
AccessioniPrimary (citable) accession number: Q5TCQ9
Secondary accession number(s): A0A024R0E9
, A0A024R0H3, Q5TCQ8, Q5TCR0, Q9H2V6, Q9H5Y8, Q9HBC4, Q9HCD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 28, 2018
Last modified: May 23, 2018
This is version 104 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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