Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5TCH4

- CP4AM_HUMAN

UniProt

Q5TCH4 - CP4AM_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cytochrome P450 4A22

Gene

CYP4A22

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. Shows no activity towards arachidonic acid and prostaglandin A1. Lacks functional activity in the kidney and does not contribute to renal 20-hydroxyeicosatetraenoic acid (20-HETE) biosynthesis.2 Publications

Catalytic activityi

Octane + 2 reduced rubredoxin + O2 + 2 H+ = 1-octanol + 2 oxidized rubredoxin + H2O.1 Publication

Kineticsi

Vmax:15.2 µmol/min/µmol enzyme.

  1. KM=56.7 µM for laurate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei321 – 3211Heme (covalent; via 1 link)By similarity
Metal bindingi457 – 4571Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. alkane 1-monooxygenase activity Source: UniProtKB-EC
  2. arachidonic acid omega-hydroxylase activity Source: UniProtKB-EC
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_13425. Miscellaneous substrates.
REACT_13645. Eicosanoids.
REACT_13814. Fatty acids.
REACT_150420. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A22
Alternative name(s):
CYPIVA22
Fatty acid omega-hydroxylase
Lauric acid omega-hydroxylase (EC:1.14.15.3)
Gene namesi
Name:CYP4A22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:20575. CYP4A22.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134979692.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 44By similarityPRO_0000343408
Chaini5 – 519515Cytochrome P450 4A22PRO_0000343409Add
BLAST

Proteomic databases

PaxDbiQ5TCH4.
PRIDEiQ5TCH4.

PTM databases

PhosphoSiteiQ5TCH4.

Expressioni

Gene expression databases

BgeeiQ5TCH4.
ExpressionAtlasiQ5TCH4. baseline.
GenevestigatoriQ5TCH4.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000360958.

Structurei

3D structure databases

ProteinModelPortaliQ5TCH4.
SMRiQ5TCH4. Positions 61-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 318Poly-Leu
Compositional biasi131 – 1344Poly-Leu

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ5TCH4.
KOiK17688.
OMAiKSAFSHQ.
PhylomeDBiQ5TCH4.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5TCH4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSVLSPSR RLGGVSGILQ VTSLLILLLL LIKAAQLYLH RQWLLKALQQ
60 70 80 90 100
FPCPPSHWLF GHIQEFQHDQ ELQRIQERVK TFPSACPYWI WGGKVRVQLY
110 120 130 140 150
DPDYMKVILG RSDPKSHGSY KFLAPRIGYG LLLLNGQTWF QHRRMLTPAF
160 170 180 190 200
HNDILKPYVG LMADSVRVML DKWEELLGQD SPLEVFQHVS LMTLDTIMKS
210 220 230 240 250
AFSHQGSIQV DRNSQSYIQA ISDLNSLVFC CMRNAFHEND TIYSLTSAGR
260 270 280 290 300
WTHRACQLAH QHTDQVIQLR KAQLQKEGEL EKIKRKRHLD FLDILLLAKM
310 320 330 340 350
ENGSILSDKD LRAEVDTFMF EGHDTTASGI SWILYALATH PKHQERCREE
360 370 380 390 400
IHGLLGDGAS ITWNHLDQMP YTTMCIKEAL RLYPPVPGIG RELSTPVTFP
410 420 430 440 450
DGRSLPKGIM VLLSIYGLHH NPKVWPNLEV FDPSRFAPGS AQHSHAFLPF
460 470 480 490 500
SGGSRNCIGK QFAMNQLKVA RALTLLRFEL LPDPTRIPIP MARLVLKSKN
510
GIHLRLRRLP NPCEDKDQL
Length:519
Mass (Da):59,246
Last modified:December 21, 2004 - v1
Checksum:iAA96444A38EE79BC
GO
Isoform 2 (identifier: Q5TCH4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     356-519: Missing.

Show »
Length:355
Mass (Da):40,922
Checksum:i392C812A7C120920
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → A in AAQ21368. (PubMed:15611369)Curated
Sequence conflicti114 – 1141P → S in AAQ21368. (PubMed:15611369)Curated
Sequence conflicti225 – 2251N → S in AAQ21368. (PubMed:15611369)Curated
Sequence conflicti232 – 2321M → V in AAQ21367. (PubMed:15611369)Curated
Sequence conflicti238 – 2381E → Q in AAQ21367. (PubMed:15611369)Curated
Sequence conflicti241 – 2411T → A in AAQ21368. (PubMed:15611369)Curated
Sequence conflicti353 – 3531G → S in AAQ21367. (PubMed:15611369)Curated
Sequence conflicti434 – 4341S → F in AAQ21367. (PubMed:15611369)Curated
Sequence conflicti487 – 4871I → T in AAQ21367. (PubMed:15611369)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111R → C in allele CYP4A22*2 and CYP4A22*3. 1 Publication
Corresponds to variant rs76011927 [ dbSNP | Ensembl ].
VAR_044349
Natural varianti104 – 1041Y → F.
Corresponds to variant rs61507155 [ dbSNP | Ensembl ].
VAR_061045
Natural varianti121 – 1211K → R.1 Publication
Corresponds to variant rs2758717 [ dbSNP | Ensembl ].
VAR_044350
Natural varianti126 – 1261R → W in allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs12564525 [ dbSNP | Ensembl ].
VAR_044351
Natural varianti130 – 1301G → S in allele CYP4A22*4, allele CYP4A22*10, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs2056900 [ dbSNP | Ensembl ].
VAR_044352
Natural varianti152 – 1521N → Y in allele CYP4A22*2, allele CYP4A22*3, allele CYP4A22*4, allele CYP4A22*5, allele CYP4A22*6, allele CYP4A22*7, allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs2056899 [ dbSNP | Ensembl ].
VAR_044353
Natural varianti185 – 1851V → F in allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 1 Publication
Corresponds to variant rs113777592 [ dbSNP | Ensembl ].
VAR_044354
Natural varianti226 – 2261S → N.1 Publication
Corresponds to variant rs35202523 [ dbSNP | Ensembl ].
VAR_044355
Natural varianti230 – 2301C → S.1 Publication
Corresponds to variant rs35156123 [ dbSNP | Ensembl ].
VAR_044356
Natural varianti231 – 2311C → R Allele CYP4A22*2, allele CYP4A22*3, allele CYP4A22*4, allele CYP4A22*5, allele CYP4A22*6, allele CYP4A22*7, allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs10789501 [ dbSNP | Ensembl ].
VAR_044357
Natural varianti276 – 2761K → T in allele CYP4A22*8, allele CYP4A22*11, allele CYP4A22*14 and allele CYP4A22*15. 1 Publication
Corresponds to variant rs149718343 [ dbSNP | Ensembl ].
VAR_044358
Natural varianti428 – 4281L → P in allele CYP4A22*6, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*12, allele CYP4A22*13 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs2405599 [ dbSNP | Ensembl ].
VAR_044359
Natural varianti491 – 4911M → I.1 Publication
Corresponds to variant rs2758714 [ dbSNP | Ensembl ].
VAR_044360
Natural varianti509 – 5091L → F in allele CYP4A22*7, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 Publications
Corresponds to variant rs4926600 [ dbSNP | Ensembl ].
VAR_044361

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei356 – 519164Missing in isoform 2. CuratedVSP_034584Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208532 Genomic DNA. Translation: AAF76722.1.
AY280371 mRNA. Translation: AAQ21367.1.
AY280372 mRNA. Translation: AAQ21368.1.
AL135960 Genomic DNA. Translation: CAI19737.1.
AL135960 Genomic DNA. Translation: CAI19738.1.
BC148248 mRNA. Translation: AAI48249.1.
CCDSiCCDS30707.1. [Q5TCH4-1]
RefSeqiNP_001010969.2. NM_001010969.2. [Q5TCH4-1]
UniGeneiHs.726474.

Genome annotation databases

EnsembliENST00000294337; ENSP00000294337; ENSG00000162365.
ENST00000371891; ENSP00000360958; ENSG00000162365. [Q5TCH4-1]
GeneIDi284541.
KEGGihsa:284541.
UCSCiuc001cqv.1. human. [Q5TCH4-1]

Polymorphism databases

DMDMi74746067.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Cytochrome P450 Allele Nomenclature Committee

CYP4A22 alleles

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208532 Genomic DNA. Translation: AAF76722.1 .
AY280371 mRNA. Translation: AAQ21367.1 .
AY280372 mRNA. Translation: AAQ21368.1 .
AL135960 Genomic DNA. Translation: CAI19737.1 .
AL135960 Genomic DNA. Translation: CAI19738.1 .
BC148248 mRNA. Translation: AAI48249.1 .
CCDSi CCDS30707.1. [Q5TCH4-1 ]
RefSeqi NP_001010969.2. NM_001010969.2. [Q5TCH4-1 ]
UniGenei Hs.726474.

3D structure databases

ProteinModelPortali Q5TCH4.
SMRi Q5TCH4. Positions 61-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000360958.

PTM databases

PhosphoSitei Q5TCH4.

Polymorphism databases

DMDMi 74746067.

Proteomic databases

PaxDbi Q5TCH4.
PRIDEi Q5TCH4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000294337 ; ENSP00000294337 ; ENSG00000162365 .
ENST00000371891 ; ENSP00000360958 ; ENSG00000162365 . [Q5TCH4-1 ]
GeneIDi 284541.
KEGGi hsa:284541.
UCSCi uc001cqv.1. human. [Q5TCH4-1 ]

Organism-specific databases

CTDi 284541.
GeneCardsi GC01P047603.
H-InvDB HIX0028676.
HGNCi HGNC:20575. CYP4A22.
MIMi 615341. gene.
neXtProti NX_Q5TCH4.
PharmGKBi PA134979692.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118816.
HOGENOMi HOG000233833.
HOVERGENi HBG000182.
InParanoidi Q5TCH4.
KOi K17688.
OMAi KSAFSHQ.
PhylomeDBi Q5TCH4.
TreeFami TF105088.

Enzyme and pathway databases

Reactomei REACT_13425. Miscellaneous substrates.
REACT_13645. Eicosanoids.
REACT_13814. Fatty acids.
REACT_150420. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Miscellaneous databases

GeneWikii CYP4A22.
GenomeRNAii 284541.
NextBioi 94950.
PROi Q5TCH4.
SOURCEi Search...

Gene expression databases

Bgeei Q5TCH4.
ExpressionAtlasi Q5TCH4. baseline.
Genevestigatori Q5TCH4.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human fatty acid omega-hydroxylase, CYP4A11: determination of complete genomic sequence and characterization of purified recombinant protein."
    Kawashima H., Naganuma T., Kusunose E., Kono T., Yasumoto R., Sugimura K., Kishimoto T.
    Arch. Biochem. Biophys. 378:333-339(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, VARIANTS ARG-121 AND ILE-491.
  2. "Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension."
    Gainer J.V., Bellamine A., Dawson E.P., Womble K.E., Grant S.W., Wang Y., Cupples L.A., Guo C.-Y., Demissie S., O'Donnell C.J., Brown N.J., Waterman M.R., Capdevila J.H.
    Circulation 111:63-69(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, VARIANTS TRP-126; SER-130; TYR-152; ASN-226; SER-230; ARG-231; PRO-428 AND PHE-509.
    Tissue: Kidney and Liver.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Genetic polymorphisms and haplotype structures of the CYP4A22 gene in a Japanese population."
    Hiratsuka M., Nozawa H., Katsumoto Y., Moteki T., Sasaki T., Konno Y., Mizugaki M.
    Mutat. Res. 599:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS CYS-11; TRP-126; SER-130; TYR-152; PHE-185; ARG-231; THR-276; PRO-428 AND PHE-509.

Entry informationi

Entry nameiCP4AM_HUMAN
AccessioniPrimary (citable) accession number: Q5TCH4
Secondary accession number(s): Q5TCH3
, Q6JXK7, Q6JXK8, Q9NRM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: December 21, 2004
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally termed CYP4A11.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3