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Q5TC82 (RC3H1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Roquin-1

Short name=Roquin
Alternative name(s):
RING finger and C3H zinc finger protein 1
RING finger and CCCH-type zinc finger domain-containing protein 1
RING finger protein 198
Gene names
Name:RC3H1
Synonyms:KIAA2025, RNF198
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1133 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs By similarity. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs By similarity. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity By similarity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression By similarity. Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation By similarity.

Subunit structure

Interacts with DDX6 and EDC4 By similarity. Interacts with CCR4-NOT deadenylase complex By similarity.

Subcellular location

CytoplasmP-body By similarity. Note: During stress, such as that induced by arsenite, localizes to cytosolic stress granules By similarity.

Tissue specificity

Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. Ref.3

Domain

The RING-type zinc finger is required for proper localization to stress granules, but not to P-bodies By similarity.

The ROQ region is required for CDE RNA-binding By similarity. It may also be involved in localization to stress granules By similarity.

Miscellaneous

The RING finger is highly conserved among the paralogs and among species, suggesting it may possess E3 ubiquitin ligase activity. A C. elegans homolog, rle-1, is involved in daf-16 ubiquitin-dependent degradation. Although such an activity has not be observed in mammalian cells so far, it is conceivable that RC3H1 may utilize E3 ubiquitin ligase activity in the promotion of mRNA decay (Ref.5).

Sequence similarities

Contains 1 C3H1-type zinc finger.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence BAC04186.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAH70709.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAH70710.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI19417.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI19419.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcytoplasmic mRNA processing body assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of B cell proliferation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of T-helper cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of activated T cell proliferation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of germinal center formation

Inferred from sequence or structural similarity. Source: BHF-UCL

nuclear-transcribed mRNA catabolic process

Inferred from sequence or structural similarity. Source: BHF-UCL

posttranscriptional regulation of gene expression

Inferred from sequence or structural similarity. Source: BHF-UCL

protein ubiquitination

Traceable author statement PubMed 19217324. Source: GOC

regulation of T cell receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of germinal center formation

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of mRNA stability

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentcytoplasmic mRNA processing body

Inferred from sequence or structural similarity. Source: BHF-UCL

cytoplasmic stress granule

Inferred from sequence or structural similarity. Source: BHF-UCL

   Molecular_functionmRNA 3'-UTR binding

Inferred from sequence or structural similarity. Source: BHF-UCL

poly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

ubiquitin-protein transferase activity

Traceable author statement PubMed 19217324. Source: BHF-UCL

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5TC82-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5TC82-2)

The sequence of this isoform differs from the canonical sequence as follows:
     988-996: Missing.
     1084-1084: S → SS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11331133Roquin-1
PRO_0000055965

Regions

Zinc finger14 – 5441RING-type; degenerate
Zinc finger413 – 44129C3H1-type
Region181 – 23252ROQ
Compositional bias533 – 788256Pro-rich

Amino acid modifications

Modified residue5351Phosphoserine Ref.4
Modified residue11101Phosphoserine By similarity

Natural variations

Alternative sequence988 – 9969Missing in isoform 2.
VSP_015015
Alternative sequence10841S → SS in isoform 2.
VSP_015016

Experimental info

Sequence conflict9861L → P in BAC04186. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: E307838DF80A7099

FASTA1,133125,736
        10         20         30         40         50         60 
MPVQAPQWTD FLSCPICTQT FDETIRKPIS LGCGHTVCKM CLNKLHRKAC PFDQTTINTD 

        70         80         90        100        110        120 
IELLPVNSAL LQLVGAQVPE QQPITLCSGV EDTKHYEEAK KCVEELALYL KPLSSARGVG 

       130        140        150        160        170        180 
LNSTTQSVLS RPMQRKLVTL VHCQLVEEEG RIRAMRAARS LGERTVTELI LQHQNPQQLS 

       190        200        210        220        230        240 
SNLWAAVRAR GCQFLGPAMQ EEALKLVLLA LEDGSALSRK VLVLFVVQRL EPRFPQASKT 

       250        260        270        280        290        300 
SIGHVVQLLY RASCFKVTKR DEDSSLMQLK EEFRTYEALR REHDSQIVQI AMEAGLRIAP 

       310        320        330        340        350        360 
DQWSSLLYGD QSHKSHMQSI IDKLQTPASF AQSVQELTIA LQRTGDPANL NRLRPHLELL 

       370        380        390        400        410        420 
ANIDPSPDAP PPTWEQLENG LVAVRTVVHG LVDYIQNHSK KGADQQQPPQ HSKYKTYMCR 

       430        440        450        460        470        480 
DMKQRGGCPR GASCTFAHSQ EELEKFRKMN KRLVPRRPLS ASLGQLNEVG LPSAAILPDE 

       490        500        510        520        530        540 
GAVDLPSRKP PALPNGIVST GNTVTQLIPR GTDPSYDSSL KPGKIDHLSS SAPGSPPDLL 

       550        560        570        580        590        600 
ESVPKSISAL PVNPHSIPPR GPADLPPMPV TKPLQMVPRG SQLYPAQQTD VYYQDPRGAA 

       610        620        630        640        650        660 
PPFEPAPYQQ GMYYTPPPQC VSRFVRPPPS APEPAPPYLD HYPPYLQERV VNSQYGTQPQ 

       670        680        690        700        710        720 
QYPPIYPSHY DGRRVYPAPS YTREEIFRES PIPIEIPPAA VPSYVPESRE RYQQIESYYP 

       730        740        750        760        770        780 
VAPHPTQIRP SYLREPPYSR LPPPPQPHPS LDELHRRRKE IMAQLEERKV ISPPPFAPSP 

       790        800        810        820        830        840 
TLPPTFHPEE FLDEDLKVAG KYKGNDYSQY SPWSCDTIGS YIGTKDAKPK DVVAAGSVEM 

       850        860        870        880        890        900 
MNVESKGMRD QRLDLQRRAA ETSDDDLIPF GDRPTVSRFG AISRTSKTIY QGAGPMQAMA 

       910        920        930        940        950        960 
PQGAPTKSIN ISDYSPYGTH GGWGASPYSP HQNIPSQGHF SERERISMSE VASHGKPLPS 

       970        980        990       1000       1010       1020 
AEREQLRLEL QQLNHQISQQ TQLRGLEAVS NRLVLQREAN TLAGQSQPPP PPPPKWPGMI 

      1030       1040       1050       1060       1070       1080 
SSEQLSLELH QVEREIGKRT RELSMENQCS LDMKSKLNTS KQAENGQPEP QNKVPAEDLT 

      1090       1100       1110       1120       1130 
LTFSDVPNGS ALTQENISLL SNKTSSLNLS EDPEGGGDNN DSQRSGVTPS SAP 

« Hide

Isoform 2 [UniParc].

Checksum: EFBE5179071DFA94
Show »

FASTA1,125124,842

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 589-1133 (ISOFORM 1).
Tissue: Testis.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The nucleotide sequence of a long cDNA clone isolated from human."
Nagase T., Kikuno R., Ohara O.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-1133 (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Brain.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Molecular control of Tfh-cell differentiation by Roquin family proteins."
Heissmeyer V., Vogel K.U.
Immunol. Rev. 253:273-289(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, MISCELLANEOUS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK093501 mRNA. Translation: BAC04186.1. Different initiation.
AK122948 mRNA. Translation: BAG53813.1.
AL121983, AL136170 Genomic DNA. Translation: CAH70709.1. Different initiation.
AL121983, AL136170 Genomic DNA. Translation: CAH70710.1. Different initiation.
AL136170, AL121983 Genomic DNA. Translation: CAI19417.1. Different initiation.
AL136170, AL121983 Genomic DNA. Translation: CAI19419.1. Different initiation.
AB095945 mRNA. Translation: BAC23121.1.
CCDSCCDS30940.1. [Q5TC82-1]
RefSeqNP_742068.1. NM_172071.2. [Q5TC82-1]
XP_005244976.1. XM_005244919.1. [Q5TC82-2]
UniGeneHs.30258.

3D structure databases

ProteinModelPortalQ5TC82.
SMRQ5TC82. Positions 12-59.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127186. 6 interactions.
IntActQ5TC82. 3 interactions.
MINTMINT-2809509.
STRING9606.ENSP00000258349.

PTM databases

PhosphoSiteQ5TC82.

Polymorphism databases

DMDM73621450.

Proteomic databases

MaxQBQ5TC82.
PaxDbQ5TC82.
PRIDEQ5TC82.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000258349; ENSP00000258349; ENSG00000135870. [Q5TC82-1]
ENST00000367694; ENSP00000356667; ENSG00000135870. [Q5TC82-2]
ENST00000367696; ENSP00000356669; ENSG00000135870. [Q5TC82-1]
GeneID149041.
KEGGhsa:149041.
UCSCuc001gju.4. human. [Q5TC82-1]
uc001gjv.3. human. [Q5TC82-2]

Organism-specific databases

CTD149041.
GeneCardsGC01M173900.
HGNCHGNC:29434. RC3H1.
HPAHPA027428.
HPA027434.
HPA027448.
MIM609424. gene.
neXtProtNX_Q5TC82.
PharmGKBPA142671090.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320727.
HOVERGENHBG080524.
InParanoidQ5TC82.
KOK15690.
OMAICTQTFD.
OrthoDBEOG7QNVKM.
PhylomeDBQ5TC82.
TreeFamTF317698.

Gene expression databases

ArrayExpressQ5TC82.
BgeeQ5TC82.
CleanExHS_RC3H1.
GenevestigatorQ5TC82.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
4.10.1000.10. 1 hit.
InterProIPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50103. ZF_C3H1. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi149041.
NextBio86056.
PROQ5TC82.
SOURCESearch...

Entry information

Entry nameRC3H1_HUMAN
AccessionPrimary (citable) accession number: Q5TC82
Secondary accession number(s): B3KVK1 expand/collapse secondary AC list , Q5W180, Q5W181, Q8IVE6, Q8N9V1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM