Q5TC82 (RC3H1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase Roquin
EC=6.3.2.-

Alternative name(s):
RING finger and C3H zinc finger protein 1
RING finger protein 198

Gene names

Name:	RC3H1
Synonyms:	KIAA2025, RNF198

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1 By similarity.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm By similarity. Note: Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA By similarity.
Tissue specificity	Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. Ref.3
Sequence similarities	Contains 1 C3H1-type zinc finger. Contains 1 RING-type zinc finger.
Sequence caution	The sequence BAC04186.1 differs from that shown. Reason: Erroneous initiation. The sequence CAH70709.1 differs from that shown. Reason: Erroneous initiation. The sequence CAH70710.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI19417.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI19419.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic mRNA processing body assembly Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of B cell proliferation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of T-helper cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of activated T cell proliferation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of germinal center formation Inferred from sequence or structural similarity. Source: BHF-UCL nuclear-transcribed mRNA catabolic process Inferred from sequence or structural similarity. Source: BHF-UCL regulation of T cell receptor signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL regulation of mRNA stability Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	cytoplasmic mRNA processing body Inferred from sequence or structural similarity. Source: BHF-UCL cytoplasmic stress granule Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	mRNA 3'-UTR binding Inferred from sequence or structural similarity. Source: BHF-UCL ubiquitin-protein ligase activity Traceable author statement PubMed 19217324. Source: BHF-UCL zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5TC82-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5TC82-2)

The sequence of this isoform differs from the canonical sequence as follows:
     988-996: Missing.
     1084-1084: S → SS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1133	1133	Probable E3 ubiquitin-protein ligase Roquin		PRO_0000055965
Regions
	Zinc finger	14 – 54	41	RING-type; degenerate
	Zinc finger	413 – 441	29	C3H1-type
	Compositional bias	533 – 788	256	Pro-rich
Amino acid modifications
	Modified residue	535	1	Phosphoserine Ref.4
	Modified residue	1110	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	988 – 996	9	Missing in isoform 2.		VSP_015015
	Alternative sequence	1084	1	S → SS in isoform 2.		VSP_015016
Experimental info
	Sequence conflict	986	1	L → P in BAC04186. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: E307838DF80A7099	FASTA	1,133	125,736
	10 20 30 40 50 60 MPVQAPQWTD FLSCPICTQT FDETIRKPIS LGCGHTVCKM CLNKLHRKAC PFDQTTINTD 70 80 90 100 110 120 IELLPVNSAL LQLVGAQVPE QQPITLCSGV EDTKHYEEAK KCVEELALYL KPLSSARGVG 130 140 150 160 170 180 LNSTTQSVLS RPMQRKLVTL VHCQLVEEEG RIRAMRAARS LGERTVTELI LQHQNPQQLS 190 200 210 220 230 240 SNLWAAVRAR GCQFLGPAMQ EEALKLVLLA LEDGSALSRK VLVLFVVQRL EPRFPQASKT 250 260 270 280 290 300 SIGHVVQLLY RASCFKVTKR DEDSSLMQLK EEFRTYEALR REHDSQIVQI AMEAGLRIAP 310 320 330 340 350 360 DQWSSLLYGD QSHKSHMQSI IDKLQTPASF AQSVQELTIA LQRTGDPANL NRLRPHLELL 370 380 390 400 410 420 ANIDPSPDAP PPTWEQLENG LVAVRTVVHG LVDYIQNHSK KGADQQQPPQ HSKYKTYMCR 430 440 450 460 470 480 DMKQRGGCPR GASCTFAHSQ EELEKFRKMN KRLVPRRPLS ASLGQLNEVG LPSAAILPDE 490 500 510 520 530 540 GAVDLPSRKP PALPNGIVST GNTVTQLIPR GTDPSYDSSL KPGKIDHLSS SAPGSPPDLL 550 560 570 580 590 600 ESVPKSISAL PVNPHSIPPR GPADLPPMPV TKPLQMVPRG SQLYPAQQTD VYYQDPRGAA 610 620 630 640 650 660 PPFEPAPYQQ GMYYTPPPQC VSRFVRPPPS APEPAPPYLD HYPPYLQERV VNSQYGTQPQ 670 680 690 700 710 720 QYPPIYPSHY DGRRVYPAPS YTREEIFRES PIPIEIPPAA VPSYVPESRE RYQQIESYYP 730 740 750 760 770 780 VAPHPTQIRP SYLREPPYSR LPPPPQPHPS LDELHRRRKE IMAQLEERKV ISPPPFAPSP 790 800 810 820 830 840 TLPPTFHPEE FLDEDLKVAG KYKGNDYSQY SPWSCDTIGS YIGTKDAKPK DVVAAGSVEM 850 860 870 880 890 900 MNVESKGMRD QRLDLQRRAA ETSDDDLIPF GDRPTVSRFG AISRTSKTIY QGAGPMQAMA 910 920 930 940 950 960 PQGAPTKSIN ISDYSPYGTH GGWGASPYSP HQNIPSQGHF SERERISMSE VASHGKPLPS 970 980 990 1000 1010 1020 AEREQLRLEL QQLNHQISQQ TQLRGLEAVS NRLVLQREAN TLAGQSQPPP PPPPKWPGMI 1030 1040 1050 1060 1070 1080 SSEQLSLELH QVEREIGKRT RELSMENQCS LDMKSKLNTS KQAENGQPEP QNKVPAEDLT 1090 1100 1110 1120 1130 LTFSDVPNGS ALTQENISLL SNKTSSLNLS EDPEGGGDNN DSQRSGVTPS SAP
	Isoform 2 [UniParc]. Checksum: EFBE5179071DFA94	FASTA	1,125	124,842
	10 20 30 40 50 60 MPVQAPQWTD FLSCPICTQT FDETIRKPIS LGCGHTVCKM CLNKLHRKAC PFDQTTINTD 70 80 90 100 110 120 IELLPVNSAL LQLVGAQVPE QQPITLCSGV EDTKHYEEAK KCVEELALYL KPLSSARGVG 130 140 150 160 170 180 LNSTTQSVLS RPMQRKLVTL VHCQLVEEEG RIRAMRAARS LGERTVTELI LQHQNPQQLS 190 200 210 220 230 240 SNLWAAVRAR GCQFLGPAMQ EEALKLVLLA LEDGSALSRK VLVLFVVQRL EPRFPQASKT 250 260 270 280 290 300 SIGHVVQLLY RASCFKVTKR DEDSSLMQLK EEFRTYEALR REHDSQIVQI AMEAGLRIAP 310 320 330 340 350 360 DQWSSLLYGD QSHKSHMQSI IDKLQTPASF AQSVQELTIA LQRTGDPANL NRLRPHLELL 370 380 390 400 410 420 ANIDPSPDAP PPTWEQLENG LVAVRTVVHG LVDYIQNHSK KGADQQQPPQ HSKYKTYMCR 430 440 450 460 470 480 DMKQRGGCPR GASCTFAHSQ EELEKFRKMN KRLVPRRPLS ASLGQLNEVG LPSAAILPDE 490 500 510 520 530 540 GAVDLPSRKP PALPNGIVST GNTVTQLIPR GTDPSYDSSL KPGKIDHLSS SAPGSPPDLL 550 560 570 580 590 600 ESVPKSISAL PVNPHSIPPR GPADLPPMPV TKPLQMVPRG SQLYPAQQTD VYYQDPRGAA 610 620 630 640 650 660 PPFEPAPYQQ GMYYTPPPQC VSRFVRPPPS APEPAPPYLD HYPPYLQERV VNSQYGTQPQ 670 680 690 700 710 720 QYPPIYPSHY DGRRVYPAPS YTREEIFRES PIPIEIPPAA VPSYVPESRE RYQQIESYYP 730 740 750 760 770 780 VAPHPTQIRP SYLREPPYSR LPPPPQPHPS LDELHRRRKE IMAQLEERKV ISPPPFAPSP 790 800 810 820 830 840 TLPPTFHPEE FLDEDLKVAG KYKGNDYSQY SPWSCDTIGS YIGTKDAKPK DVVAAGSVEM 850 860 870 880 890 900 MNVESKGMRD QRLDLQRRAA ETSDDDLIPF GDRPTVSRFG AISRTSKTIY QGAGPMQAMA 910 920 930 940 950 960 PQGAPTKSIN ISDYSPYGTH GGWGASPYSP HQNIPSQGHF SERERISMSE VASHGKPLPS 970 980 990 1000 1010 1020 AEREQLRLEL QQLNHQISQQ TQLRGLEREA NTLAGQSQPP PPPPPKWPGM ISSEQLSLEL 1030 1040 1050 1060 1070 1080 HQVEREIGKR TRELSMENQC SLDMKSKLNT SKQAENGQPE PQNKVPAEDL TLTFSSDVPN 1090 1100 1110 1120 GSALTQENIS LLSNKTSSLN LSEDPEGGGD NNDSQRSGVT PSSAP

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 589-1133 (ISOFORM 1). Tissue: Testis.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The nucleotide sequence of a long cDNA clone isolated from human." Nagase T., Kikuno R., Ohara O. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-1133 (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Brain.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK093501 mRNA. Translation: BAC04186.1. Different initiation. AK122948 mRNA. Translation: BAG53813.1. AL121983, AL136170 Genomic DNA. Translation: CAH70709.1. Different initiation. AL121983, AL136170 Genomic DNA. Translation: CAH70710.1. Different initiation. AL136170, AL121983 Genomic DNA. Translation: CAI19417.1. Different initiation. AL136170, AL121983 Genomic DNA. Translation: CAI19419.1. Different initiation. AB095945 mRNA. Translation: BAC23121.1.
IPI	IPI00216992. IPI00640426.
RefSeq	NP_742068.1. NM_172071.2.
UniGene	Hs.30258.
3D structure databases
ProteinModelPortal	Q5TC82.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000258349.
PTM databases
PhosphoSite	Q5TC82.
Polymorphism databases
DMDM	73621450.
Proteomic databases
PaxDb	Q5TC82.
PRIDE	Q5TC82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258349; ENSP00000258349; ENSG00000135870. ENST00000367694; ENSP00000356667; ENSG00000135870. ENST00000367696; ENSP00000356669; ENSG00000135870.
GeneID	149041.
KEGG	hsa:149041.
UCSC	uc001gju.4. human. uc001gjv.3. human.
Organism-specific databases
CTD	149041.
GeneCards	GC01M173900.
HGNC	HGNC:29434. RC3H1.
HPA	HPA027428. HPA027434. HPA027448.
MIM	609424. gene.
neXtProt	NX_Q5TC82.
PharmGKB	PA142671090.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG320727.
HOVERGEN	HBG080524.
InParanoid	Q5TC82.
KO	K15690.
OMA	TFDETIR.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q5TC82.
Bgee	Q5TC82.
CleanEx	HS_RC3H1.
Genevestigator	Q5TC82.
GermOnline	ENSG00000135870. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR000571. Znf_CCCH. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF00642. zf-CCCH. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS50103. ZF_C3H1. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	149041.
NextBio	86056.
SOURCE	Search...

Entry information

Entry name

RC3H1_HUMAN

Accession

Primary (citable) accession number: Q5TC82
Secondary accession number(s): B3KVK1 , Q5W180, Q5W181, Q8IVE6, Q8N9V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.