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Protein

DEP domain-containing protein 1A

Gene

DEPDC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DEP domain-containing protein 1A
Gene namesi
Name:DEPDC1
Synonyms:DEPDC1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:22949. DEPDC1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55635.
OpenTargetsiENSG00000024526.
PharmGKBiPA134974825.

Polymorphism and mutation databases

DMDMi300669641.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002847861 – 811DEP domain-containing protein 1AAdd BLAST811

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei512PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5TB30.
MaxQBiQ5TB30.
PaxDbiQ5TB30.
PeptideAtlasiQ5TB30.
PRIDEiQ5TB30.

PTM databases

iPTMnetiQ5TB30.
PhosphoSitePlusiQ5TB30.

Expressioni

Tissue specificityi

Expressed in testis. Up-regulated in bladder cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000024526.
CleanExiHS_DEPDC1.
ExpressionAtlasiQ5TB30. baseline and differential.
GenevisibleiQ5TB30. HS.

Organism-specific databases

HPAiCAB017845.
HPA049272.

Interactioni

Subunit structurei

Isoform 2 and isoform 5 can form homodimers and heterodimers. Interacts with ZNF224.2 Publications

Protein-protein interaction databases

BioGridi120774. 17 interactors.
IntActiQ5TB30. 6 interactors.
MINTiMINT-4995437.
STRINGi9606.ENSP00000412292.

Structurei

Secondary structure

1811
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 26Combined sources16
Beta strandi36 – 39Combined sources4
Helixi46 – 59Combined sources14
Beta strandi60 – 63Combined sources4
Turni64 – 66Combined sources3
Helixi69 – 81Combined sources13
Beta strandi84 – 89Combined sources6
Beta strandi99 – 101Combined sources3
Beta strandi103 – 105Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YSRNMR-A11-108[»]
ProteinModelPortaliQ5TB30.
SMRiQ5TB30.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5TB30.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 108DEPPROSITE-ProRule annotationAdd BLAST85
Domaini281 – 321Rho-GAPAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni598 – 653Interaction with ZNF2241 PublicationAdd BLAST56

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.Curated

Phylogenomic databases

eggNOGiENOG410IEI5. Eukaryota.
ENOG410YMM7. LUCA.
GeneTreeiENSGT00530000063030.
HOVERGENiHBG059792.
InParanoidiQ5TB30.
OMAiEEVWRYV.
OrthoDBiEOG091G04WW.
PhylomeDBiQ5TB30.
TreeFamiTF328365.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.555.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5TB30-5) [UniParc]FASTAAdd to basket
Also known as: DEPDC1-V1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESQGVPPGP YRATKLWNEV TTSFRAGMPL RKHRQHFKKY GNCFTAGEAV
60 70 80 90 100
DWLYDLLRNN SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSENVDDN
110 120 130 140 150
NQLFRFPATS PLKTLPRRYP ELRKNNIENF SKDKDSIFKL RNLSRRTPKR
160 170 180 190 200
HGLHLSQENG EKIKHEIINE DQENAIDNRE LSQEDVEEVW RYVILIYLQT
210 220 230 240 250
ILGVPSLEEV INPKQVIPQY IMYNMANTSK RGVVILQNKS DDLPHWVLSA
260 270 280 290 300
MKCLANWPRS NDMNNPTYVG FERDVFRTIA DYFLDLPEPL LTFEYYELFV
310 320 330 340 350
NILVVCGYIT VSDRSSGIHK IQDDPQSSKF LHLNNLNSFK STECLLLSLL
360 370 380 390 400
HREKNKEESD STERLQISNP GFQERCAKKM QLVNLRNRRV SANDIMGGSC
410 420 430 440 450
HNLIGLSNMH DLSSNSKPRC CSLEGIVDVP GNSSKEASSV FHQSFPNIEG
460 470 480 490 500
QNNKLFLESK PKQEFLLNLH SEENIQKPFS AGFKRTSTLT VQDQEELCNG
510 520 530 540 550
KCKSKQLCRS QSLLLRSSTR RNSYINTPVA EIIMKPNVGQ GSTSVQTAME
560 570 580 590 600
SELGESSATI NKRLCKSTIE LSENSLLPAS SMLTGTQSLL QPHLERVAID
610 620 630 640 650
ALQLCCLLLP PPNRRKLQLL MRMISRMSQN VDMPKLHDAM GTRSLMIHTF
660 670 680 690 700
SRCVLCCAEE VDLDELLAGR LVSFLMDHHQ EILQVPSYLQ TAVEKHLDYL
710 720 730 740 750
KKGHIENPGD GLFAPLPTYS YCKQISAQEF DEQKVSTSQA AIAELLENII
760 770 780 790 800
KNRSLPLKEK RKKLKQFQKE YPLIYQKRFP TTESEAALFG DKPTIKQPML
810
ILRKPKFRSL R
Length:811
Mass (Da):92,960
Last modified:July 13, 2010 - v2
Checksum:iF694E10FB79AF3B0
GO
Isoform 2 (identifier: Q5TB30-2) [UniParc]FASTAAdd to basket
Also known as: DEPDC1-V2

The sequence of this isoform differs from the canonical sequence as follows:
     304-304: V → G
     305-588: Missing.

Show »
Length:527
Mass (Da):61,493
Checksum:i8DFCF1DC7F13AFD7
GO

Sequence cautioni

The sequence AAH03511 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91201 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14246 differs from that shown. Probable cloning artifact.Curated
The sequence BAB14246 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI22441 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI22443 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti720S → P in BAA91201 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059798404I → R.Corresponds to variant rs3790479dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024652304V → G in isoform 2. 3 Publications1
Alternative sequenceiVSP_024653305 – 588Missing in isoform 2. 3 PublicationsAdd BLAST284

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278112 mRNA. Translation: CAB92444.1.
AB281187 mRNA. Translation: BAF91373.1.
AB281274 mRNA. Translation: BAF91374.1.
AL138789 Genomic DNA. Translation: CAI22441.1. Sequence problems.
AL138789 Genomic DNA. Translation: CAI22442.1.
AL138789 Genomic DNA. Translation: CAI22443.1. Sequence problems.
CH471059 Genomic DNA. Translation: EAX06475.1.
CH471059 Genomic DNA. Translation: EAX06476.1.
BC003511 mRNA. Translation: AAH03511.1. Sequence problems.
BC065304 mRNA. Translation: AAH65304.1.
AK000490 mRNA. Translation: BAA91201.1. Different initiation.
AK022792 mRNA. Translation: BAB14246.1. Sequence problems.
CCDSiCCDS44159.1. [Q5TB30-5]
CCDS644.1. [Q5TB30-2]
RefSeqiNP_001107592.1. NM_001114120.2. [Q5TB30-5]
NP_060249.2. NM_017779.5. [Q5TB30-2]
UniGeneiHs.445098.

Genome annotation databases

EnsembliENST00000370966; ENSP00000360005; ENSG00000024526. [Q5TB30-2]
ENST00000456315; ENSP00000412292; ENSG00000024526. [Q5TB30-5]
GeneIDi55635.
KEGGihsa:55635.
UCSCiuc001del.5. human. [Q5TB30-5]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278112 mRNA. Translation: CAB92444.1.
AB281187 mRNA. Translation: BAF91373.1.
AB281274 mRNA. Translation: BAF91374.1.
AL138789 Genomic DNA. Translation: CAI22441.1. Sequence problems.
AL138789 Genomic DNA. Translation: CAI22442.1.
AL138789 Genomic DNA. Translation: CAI22443.1. Sequence problems.
CH471059 Genomic DNA. Translation: EAX06475.1.
CH471059 Genomic DNA. Translation: EAX06476.1.
BC003511 mRNA. Translation: AAH03511.1. Sequence problems.
BC065304 mRNA. Translation: AAH65304.1.
AK000490 mRNA. Translation: BAA91201.1. Different initiation.
AK022792 mRNA. Translation: BAB14246.1. Sequence problems.
CCDSiCCDS44159.1. [Q5TB30-5]
CCDS644.1. [Q5TB30-2]
RefSeqiNP_001107592.1. NM_001114120.2. [Q5TB30-5]
NP_060249.2. NM_017779.5. [Q5TB30-2]
UniGeneiHs.445098.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YSRNMR-A11-108[»]
ProteinModelPortaliQ5TB30.
SMRiQ5TB30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120774. 17 interactors.
IntActiQ5TB30. 6 interactors.
MINTiMINT-4995437.
STRINGi9606.ENSP00000412292.

PTM databases

iPTMnetiQ5TB30.
PhosphoSitePlusiQ5TB30.

Polymorphism and mutation databases

DMDMi300669641.

Proteomic databases

EPDiQ5TB30.
MaxQBiQ5TB30.
PaxDbiQ5TB30.
PeptideAtlasiQ5TB30.
PRIDEiQ5TB30.

Protocols and materials databases

DNASUi55635.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370966; ENSP00000360005; ENSG00000024526. [Q5TB30-2]
ENST00000456315; ENSP00000412292; ENSG00000024526. [Q5TB30-5]
GeneIDi55635.
KEGGihsa:55635.
UCSCiuc001del.5. human. [Q5TB30-5]

Organism-specific databases

CTDi55635.
DisGeNETi55635.
GeneCardsiDEPDC1.
HGNCiHGNC:22949. DEPDC1.
HPAiCAB017845.
HPA049272.
MIMi612002. gene.
neXtProtiNX_Q5TB30.
OpenTargetsiENSG00000024526.
PharmGKBiPA134974825.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEI5. Eukaryota.
ENOG410YMM7. LUCA.
GeneTreeiENSGT00530000063030.
HOVERGENiHBG059792.
InParanoidiQ5TB30.
OMAiEEVWRYV.
OrthoDBiEOG091G04WW.
PhylomeDBiQ5TB30.
TreeFamiTF328365.

Miscellaneous databases

EvolutionaryTraceiQ5TB30.
GenomeRNAii55635.
PROiQ5TB30.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000024526.
CleanExiHS_DEPDC1.
ExpressionAtlasiQ5TB30. baseline and differential.
GenevisibleiQ5TB30. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.555.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEP1A_HUMAN
AccessioniPrimary (citable) accession number: Q5TB30
Secondary accession number(s): A8QXE0
, A8QXE1, Q05DU3, Q5TB28, Q9H9I3, Q9NX21, Q9NXZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 13, 2010
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.