Q5TAQ9 (DCAF8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DDB1- and CUL4-associated factor 8

Alternative name(s):
WD repeat-containing protein 42A

Gene names

Name:	DCAF8
Synonyms:	H326, WDR42A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.8
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DDB1, CUL4A and CUL4B. Ref.8
Sequence similarities	Belongs to the WD repeat DCAF8 family. Contains 7 WD repeats.
Sequence caution	The sequence AAA16607.1 differs from that shown. Reason: Frameshift at positions 117 and 123.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination Inferred by curator Ref.8. Source: UniProtKB
Cellular component	CUL4 RING ubiquitin ligase complex Inferred from direct assay Ref.8. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MDFI	Q99750	3	EBI-740686,EBI-724076

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5TAQ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5TAQ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     242-273: AKFLPNSGDSTLAMCARDGQVRVAELSATQCC → VRQGSIIATERIRHELEKSELQHGLGADSELL
     274-597: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 597

597

DDB1- and CUL4-associated factor 8

PRO_0000296957

Regions

Repeat

191 – 230

40

WD 1

Repeat

234 – 275

42

WD 2

Repeat

281 – 321

41

WD 3

Repeat

329 – 369

41

WD 4

Repeat

385 – 424

40

WD 5

Repeat

432 – 472

41

WD 6

Repeat

476 – 515

40

WD 7

Amino acid modifications

Modified residue

99

1

Phosphoserine Ref.7 Ref.9 Ref.10

Modified residue

129

1

Phosphoserine Ref.7 Ref.9 Ref.10

Modified residue

130

1

Phosphoserine Ref.7 Ref.9 Ref.10

Natural variations

Alternative sequence

242 – 273

32

AKFLP…ATQCC → VRQGSIIATERIRHELEKSE LQHGLGADSELL in isoform 2.

VSP_027264

Alternative sequence

274 – 597

324

Missing in isoform 2.

VSP_027265

Experimental info

Mutagenesis

314

1

R → A: Reduces association with DDB1. Ref.8

Mutagenesis

362

1

R → A: Reduces association with DDB1. Ref.8

Sequence conflict

68

1

D → E in AAH13107. Ref.6

Sequence conflict

123

1

A → T in AAH98271. Ref.6

Sequence conflict

155

1

L → F in CAH18661. Ref.3

Sequence conflict

181 – 182

2

QR → HG in AAA16607. Ref.1

Sequence conflict

237

1

S → G in BAD97195. Ref.2

Sequence conflict

375

1

K → E in CAH18661. Ref.3

Secondary structure

1

.

.

.

597

Helix Strand Turn

Details...

Turn

156 – 160

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 3F3396C77EA1E3AD	FASTA	597	66,852
	10 20 30 40 50 60 MSSKGSSTDG RTDLANGSLS SSPEEMSGAE EGRETSSGIE VEASDLSLSL TGDDGGPNRT 70 80 90 100 110 120 STESRGTDTE SSGEDKDSDS MEDTGHYSIN DENRVHDRSE EEEEEEEEEE EEQPRRRVQR 130 140 150 160 170 180 KRANRDQDSS DDERALEDWV SSETSALPRP RWQALPALRE RELGSSARFV YEACGARVFV 190 200 210 220 230 240 QRFRLQHGLE GHTGCVNTLH FNQRGTWLAS GSDDLKVVVW DWVRRQPVLD FESGHKSNVF 250 260 270 280 290 300 QAKFLPNSGD STLAMCARDG QVRVAELSAT QCCKNTKRVA QHKGASHKLA LEPDSPCTFL 310 320 330 340 350 360 SAGEDAVVFT IDLRQDRPAS KLVVTKEKEK KVGLYTIYVN PANTHQFAVG GRDQFVRIYD 370 380 390 400 410 420 QRKIDENENN GVLKKFCPHH LVNSESKANI TCLVYSHDGT ELLASYNDED IYLFNSSHSD 430 440 450 460 470 480 GAQYVKRYKG HRNNATVKGV NFYGPKSEFV VSGSDCGHIF LWEKSSCQII QFMEGDKGGV 490 500 510 520 530 540 VNCLEPHPHL PVLATSGLDH DVKIWAPTAE ASTELTGLKD VIKKNKRERD EDSLHQTDLF 550 560 570 580 590 DSHMLWFLMH HLRQRRHHRR WREPGVGATD ADSDESPSSS DTSDEEEGPD RVQCMPS
	Isoform 2 [UniParc]. Checksum: 160E9D57D87F6B51	FASTA	273	30,569
	10 20 30 40 50 60 MSSKGSSTDG RTDLANGSLS SSPEEMSGAE EGRETSSGIE VEASDLSLSL TGDDGGPNRT 70 80 90 100 110 120 STESRGTDTE SSGEDKDSDS MEDTGHYSIN DENRVHDRSE EEEEEEEEEE EEQPRRRVQR 130 140 150 160 170 180 KRANRDQDSS DDERALEDWV SSETSALPRP RWQALPALRE RELGSSARFV YEACGARVFV 190 200 210 220 230 240 QRFRLQHGLE GHTGCVNTLH FNQRGTWLAS GSDDLKVVVW DWVRRQPVLD FESGHKSNVF 250 260 270 QVRQGSIIAT ERIRHELEKS ELQHGLGADS ELL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"H326 is a human gene homologous to murine PC326 that is ubiquitously expressed, and has a murine homologue that is also ubiquitously expressed." Bergsagel P.L., Kuehl W. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Myeloma.
[2]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Synovium.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon carcinoma.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Skin.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-129 AND SER-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006) [PubMed: 16949367] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDB1; CUL4A AND CUL4B, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF ARG-314 AND ARG-362.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-129 AND SER-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-129 AND SER-130, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U06631 mRNA. Translation: AAA16607.1. Frameshift.
AK223475 mRNA. Translation: BAD97195.1.
CR749801 mRNA. Translation: CAH18661.1.
AL139011 Genomic DNA. Translation: CAI15993.1.
CH471121 Genomic DNA. Translation: EAW52731.1.
CH471121 Genomic DNA. Translation: EAW52732.1.
BC013107 mRNA. Translation: AAH13107.1.
BC080597 mRNA. Translation: AAH80597.1.
BC098271 mRNA. Translation: AAH98271.1.
BC099709 mRNA. Translation: AAH99709.1.
BC099846 mRNA. Translation: AAH99846.1.
BC111063 mRNA. Translation: AAI11064.1.

IPI

IPI00290071.
IPI00854775.

RefSeq

NP_056541.2. NM_015726.3.

UniGene

Hs.632447.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I8E	X-ray	3.40	C/D	153-165	[»]

ProteinModelPortal

Q5TAQ9.

SMR

Q5TAQ9. Positions 158-508.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48762N.

IntAct

Q5TAQ9. 4 interactions.

MINT

MINT-1446617.

STRING

Q5TAQ9.

PTM databases

PhosphoSite

Q5TAQ9.

Polymorphism databases

DMDM

74756455.

Proteomic databases

PRIDE

Q5TAQ9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000326837; ENSP00000318227; ENSG00000132716.
ENST00000368073; ENSP00000357052; ENSG00000132716.
ENST00000368074; ENSP00000357053; ENSG00000132716.

GeneID

50717.

KEGG

hsa:50717.

UCSC

uc001fvn.1. human.
uc001fvp.2. human.

Organism-specific databases

CTD

50717.

GeneCards

GC01M160185.

H-InvDB

HIX0020510.
HIX0028423.

HGNC

HGNC:24891. DCAF8.

HPA

HPA027218.
HPA027381.

neXtProt

NX_Q5TAQ9.

PharmGKB

PA134953598.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00530000062951.

HOVERGEN

HBG053807.

InParanoid

Q5TAQ9.

OMA

GAQYIKR.

OrthoDB

EOG47H5PN.

PhylomeDB

Q5TAQ9.

Gene expression databases

ArrayExpress

Q5TAQ9.

Bgee

Q5TAQ9.

CleanEx

HS_WDR42A.

Genevestigator

Q5TAQ9.

Family and domain databases

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.

KO

K11804.

Pfam

PF00400. WD40. 4 hits.
[Graphical view]

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

53224.

Entry information

Entry name

DCAF8_HUMAN

Accession

Primary (citable) accession number: Q5TAQ9
Secondary accession number(s): D3DVE6 , Q12839, Q4QQI6, Q53F14, Q66K50, Q68CS7, Q96E00

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	December 21, 2004
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.