Q5TA89 (HES5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor HES-5

Alternative name(s):
Class B basic helix-loop-helix protein 38
Short name=bHLHb38
Hairy and enhancer of split 5

Gene names

Name:	HES5
Synonyms:	BHLHB38

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional repressor of genes that require a bHLH protein for their transcription By similarity.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in fetal heart and brain tumors. Ref.4
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG) By similarity. The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins By similarity.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from mutant phenotype PubMed 19682396PubMed 19855400. Source: UniProtKB S-shaped body morphogenesis Inferred from electronic annotation. Source: Compara astrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB auditory receptor cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB auditory receptor cell fate determination Inferred from electronic annotation. Source: Compara camera-type eye development Inferred from sequence or structural similarity. Source: UniProtKB cartilage development Inferred from direct assay PubMed 17093926. Source: UniProtKB cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB cell maturation Inferred from sequence or structural similarity. Source: UniProtKB central nervous system myelination Inferred from sequence or structural similarity. Source: UniProtKB comma-shaped body morphogenesis Inferred from electronic annotation. Source: Compara forebrain radial glial cell differentiation Inferred from electronic annotation. Source: Compara glial cell fate commitment Inferred from sequence or structural similarity. Source: UniProtKB metanephric nephron tubule morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of astrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of forebrain neuron differentiation Inferred from electronic annotation. Source: Compara negative regulation of inner ear receptor cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of oligodendrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of pro-B cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of stem cell differentiation Inferred from mutant phenotype PubMed 19682396. Source: UniProtKB neural tube development Inferred from sequence or structural similarity. Source: UniProtKB neuronal stem cell maintenance Inferred from expression pattern PubMed 19682396. Source: UniProtKB positive regulation of BMP signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Notch signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from genetic interaction PubMed 19855400. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from sequence or structural similarity. Source: UniProtKB protein complex assembly Inferred from sequence or structural similarity. Source: UniProtKB regulation of myelination Inferred from sequence or structural similarity. Source: UniProtKB smoothened signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB specification of loop of Henle identity Inferred from electronic annotation. Source: Compara telencephalon development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from sequence or structural similarity. Source: UniProtKB chromatin binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara transcription factor binding Non-traceable author statement PubMed 17611704. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Transcription factor HES-5		PRO_0000269175
Regions
	Domain	16 – 72	57	bHLH
	Domain	88 – 119	32	Orange
	Motif	163 – 166	4	WRPW motif
	Compositional bias	14 – 31	18	Arg/Lys-rich (basic)
	Compositional bias	122 – 142	21	Pro-rich
	Compositional bias	147 – 153	7	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q5TA89 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 6B0ECF2482EBE43B	FASTA	166	18,226
	10 20 30 40 50 60 MAPSTVAVEL LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH QPNSKLEKAD 70 80 90 100 110 120 ILEMAVSYLK HSKAFVAAAG PKSLHQDYSE GYSWCLQEAV QFLTLHAASD TQMKLLYHFQ 130 140 150 160 RPPAAPAAPA KEPKAPGAAP PPALSAKATA AAAAAHQPAC GLWRPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Wistow G. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Identification and characterization of human HES2, HES3, and HES5 genes in silico." Katoh M., Katoh M. Int. J. Oncol. 25:529-534(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ272660 mRNA. Translation: ABB83829.1. AL139246 Genomic DNA. Translation: CAX30820.1. CH471183 Genomic DNA. Translation: EAW56096.1.
IPI	IPI00373977.
RefSeq	NP_001010926.1. NM_001010926.3.
UniGene	Hs.57971.
3D structure databases
ProteinModelPortal	Q5TA89.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000367714.
PTM databases
PhosphoSite	Q5TA89.
Polymorphism databases
DMDM	74745795.
Proteomic databases
PaxDb	Q5TA89.
PRIDE	Q5TA89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378453; ENSP00000367714; ENSG00000197921.
GeneID	388585.
KEGG	hsa:388585.
UCSC	uc001ajn.3. human.
Organism-specific databases
CTD	388585.
GeneCards	GC01M002450.
HGNC	HGNC:19764. HES5.
MIM	607348. gene.
neXtProt	NX_Q5TA89.
PharmGKB	PA134967985.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG83691.
HOGENOM	HOG000236346.
HOVERGEN	HBG005960.
InParanoid	Q5TA89.
KO	K06055.
OMA	QFLTLHS.
OrthoDB	EOG495ZT5.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q5TA89.
CleanEx	HS_HES5.
Genevestigator	Q5TA89.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. IPR003650. Orange. [Graphical view]
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	388585.
NextBio	102194.
SOURCE	Search...

Entry information

Entry name

HES5_HUMAN

Accession

Primary (citable) accession number: Q5TA89
Secondary accession number(s): B9DI85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.