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Protein

Small RNA 2'-O-methyltransferase

Gene

HENMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + small RNA = S-adenosyl-L-homocysteine + small RNA containing a 3'-terminal 2'-O-methylnucleotide.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60S-adenosyl-L-methionineBy similarity1
Binding sitei78S-adenosyl-L-methionineBy similarity1
Binding sitei114S-adenosyl-L-methionineBy similarity1
Metal bindingi132MagnesiumBy similarity1
Metal bindingi135MagnesiumBy similarity1
Metal bindingi136Magnesium; via tele nitrogenBy similarity1
Metal bindingi181Magnesium; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Metal-binding, RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Small RNA 2'-O-methyltransferase (EC:2.1.1.n8)
Alternative name(s):
HEN1 methyltransferase homolog 1
Gene namesi
Name:HENMT1
Synonyms:C1orf59
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26400. HENMT1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi113802.
OpenTargetsiENSG00000162639.
PharmGKBiPA128394748.

Polymorphism and mutation databases

BioMutaiHENMT1.
DMDMi74745527.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003041391 – 393Small RNA 2'-O-methyltransferaseAdd BLAST393

Proteomic databases

EPDiQ5T8I9.
MaxQBiQ5T8I9.
PaxDbiQ5T8I9.
PeptideAtlasiQ5T8I9.
PRIDEiQ5T8I9.

PTM databases

iPTMnetiQ5T8I9.
PhosphoSitePlusiQ5T8I9.

Expressioni

Gene expression databases

BgeeiENSG00000162639.
CleanExiHS_C1orf59.
ExpressionAtlasiQ5T8I9. baseline and differential.
GenevisibleiQ5T8I9. HS.

Organism-specific databases

HPAiHPA028464.
HPA028497.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
taxP140793EBI-9675710,EBI-9675698From a different organism.
TRAF2Q129333EBI-9675710,EBI-355744

Protein-protein interaction databases

BioGridi125261. 9 interactors.
IntActiQ5T8I9. 2 interactors.
STRINGi9606.ENSP00000359049.

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 46Combined sources16
Beta strandi49 – 54Combined sources6
Helixi60 – 65Combined sources6
Beta strandi72 – 79Combined sources8
Helixi82 – 84Combined sources3
Helixi89 – 91Combined sources3
Helixi95 – 99Combined sources5
Beta strandi102 – 104Combined sources3
Beta strandi107 – 112Combined sources6
Helixi120 – 122Combined sources3
Beta strandi126 – 132Combined sources7
Helixi134 – 136Combined sources3
Helixi139 – 149Combined sources11
Turni150 – 153Combined sources4
Beta strandi156 – 163Combined sources8
Helixi165 – 167Combined sources3
Helixi187 – 200Combined sources14
Beta strandi203 – 210Combined sources8
Helixi218 – 220Combined sources3
Beta strandi223 – 231Combined sources9
Beta strandi250 – 257Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XCXX-ray2.84A14-262[»]
ProteinModelPortaliQ5T8I9.
SMRiQ5T8I9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1045. Eukaryota.
ENOG410XSD6. LUCA.
GeneTreeiENSGT00390000004798.
HOGENOMiHOG000049068.
HOVERGENiHBG097349.
KOiK20798.
PhylomeDBiQ5T8I9.
TreeFamiTF315178.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026610. Hen1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21404. PTHR21404. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5T8I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENNLQCSS VVDGNFEEVP RETAIQFKPP LYRQRYQFVK NLVDQHEPKK
60 70 80 90 100
VADLGCGDTS LLRLLKVNPC IELLVGVDIN EDKLRWRGDS LAPFLGDFLK
110 120 130 140 150
PRDLNLTITL YHGSVVERDS RLLGFDLITC IELIEHLDSG DLARFPEVVF
160 170 180 190 200
GYLSPSMIVI STPNSEFNPL FPSVTLRDSD HKFEWTRMEF QTWALYVANR
210 220 230 240 250
YDYSVEFTGV GEPPAGAENV GYCTQIGIFR KNGGKATESC LSEQHDQHVY
260 270 280 290 300
KAVFTTSYPS LQQERFFKLV LVNEVSQQVE SLRVSHLPRR KEQAGERGDK
310 320 330 340 350
PKDIGGSKAP VPCFGPVFTE VEKAKIENSP TPFCVGDKFF VPLQRLLAYP
360 370 380 390
KLNRLCANEE MMRSVIADSI PLSSDGSAVV ADLRNYFDEQ FEF
Length:393
Mass (Da):44,525
Last modified:December 21, 2004 - v1
Checksum:i897A46664A44F6C5
GO

Sequence cautioni

The sequence CAI12930 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI12931 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti154S → F in BAB70852 (PubMed:14702039).Curated1
Sequence conflicti365V → A in BAB70852 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035017129T → A.Corresponds to variant rs9988420dbSNPEnsembl.1
Natural variantiVAR_035018230R → Q.Corresponds to variant rs35974434dbSNPEnsembl.1
Natural variantiVAR_035019361M → I.1 PublicationCorresponds to variant rs17850887dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055087 mRNA. Translation: BAB70852.1.
AL160171 Genomic DNA. Translation: CAI12929.1.
AL160171 Genomic DNA. Translation: CAI12930.1. Sequence problems.
AL160171 Genomic DNA. Translation: CAI12931.1. Sequence problems.
CH471122 Genomic DNA. Translation: EAW56326.1.
BC012198 mRNA. Translation: AAH12198.1.
BC088366 mRNA. Translation: AAH88366.1.
CCDSiCCDS787.1.
RefSeqiNP_001096062.1. NM_001102592.1.
NP_653185.2. NM_144584.2.
UniGeneiHs.7962.

Genome annotation databases

EnsembliENST00000370032; ENSP00000359049; ENSG00000162639.
ENST00000402983; ENSP00000385655; ENSG00000162639.
GeneIDi113802.
KEGGihsa:113802.
UCSCiuc001dvt.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055087 mRNA. Translation: BAB70852.1.
AL160171 Genomic DNA. Translation: CAI12929.1.
AL160171 Genomic DNA. Translation: CAI12930.1. Sequence problems.
AL160171 Genomic DNA. Translation: CAI12931.1. Sequence problems.
CH471122 Genomic DNA. Translation: EAW56326.1.
BC012198 mRNA. Translation: AAH12198.1.
BC088366 mRNA. Translation: AAH88366.1.
CCDSiCCDS787.1.
RefSeqiNP_001096062.1. NM_001102592.1.
NP_653185.2. NM_144584.2.
UniGeneiHs.7962.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XCXX-ray2.84A14-262[»]
ProteinModelPortaliQ5T8I9.
SMRiQ5T8I9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125261. 9 interactors.
IntActiQ5T8I9. 2 interactors.
STRINGi9606.ENSP00000359049.

PTM databases

iPTMnetiQ5T8I9.
PhosphoSitePlusiQ5T8I9.

Polymorphism and mutation databases

BioMutaiHENMT1.
DMDMi74745527.

Proteomic databases

EPDiQ5T8I9.
MaxQBiQ5T8I9.
PaxDbiQ5T8I9.
PeptideAtlasiQ5T8I9.
PRIDEiQ5T8I9.

Protocols and materials databases

DNASUi113802.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370032; ENSP00000359049; ENSG00000162639.
ENST00000402983; ENSP00000385655; ENSG00000162639.
GeneIDi113802.
KEGGihsa:113802.
UCSCiuc001dvt.5. human.

Organism-specific databases

CTDi113802.
DisGeNETi113802.
GeneCardsiHENMT1.
HGNCiHGNC:26400. HENMT1.
HPAiHPA028464.
HPA028497.
MIMi612178. gene.
neXtProtiNX_Q5T8I9.
OpenTargetsiENSG00000162639.
PharmGKBiPA128394748.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1045. Eukaryota.
ENOG410XSD6. LUCA.
GeneTreeiENSGT00390000004798.
HOGENOMiHOG000049068.
HOVERGENiHBG097349.
KOiK20798.
PhylomeDBiQ5T8I9.
TreeFamiTF315178.

Enzyme and pathway databases

ReactomeiR-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Miscellaneous databases

ChiTaRSiHENMT1. human.
GenomeRNAii113802.
PROiQ5T8I9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162639.
CleanExiHS_C1orf59.
ExpressionAtlasiQ5T8I9. baseline and differential.
GenevisibleiQ5T8I9. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026610. Hen1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21404. PTHR21404. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHENMT_HUMAN
AccessioniPrimary (citable) accession number: Q5T8I9
Secondary accession number(s): A8MRR6
, B1AM16, B1AM17, Q96EJ7, Q96NN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.