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Protein

Small RNA 2'-O-methyltransferase

Gene

HENMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.By similarity

Catalytic activityi

S-adenosyl-L-methionine + small RNA = S-adenosyl-L-homocysteine + small RNA containing a 3'-terminal 2'-O-methylnucleotide.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei55S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication1
Binding sitei78S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei83S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei115S-adenosyl-L-methionine; via amide nitrogenCombined sources1
Binding sitei131S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1
Metal bindingi132MagnesiumBy similarity1
Metal bindingi135MagnesiumBy similarity1
Metal bindingi136Magnesium; via tele nitrogenBy similarity1
Metal bindingi181Magnesium; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processRNA-mediated gene silencing
LigandMetal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Small RNA 2'-O-methyltransferase (EC:2.1.1.n8By similarity)
Alternative name(s):
HEN1 methyltransferase homolog 1
Gene namesi
Name:HENMT1
Synonyms:C1orf59
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162639.15
HGNCiHGNC:26400 HENMT1
MIMi612178 gene
neXtProtiNX_Q5T8I9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi113802
OpenTargetsiENSG00000162639
PharmGKBiPA128394748

Polymorphism and mutation databases

BioMutaiHENMT1
DMDMi74745527

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003041391 – 393Small RNA 2'-O-methyltransferaseAdd BLAST393

Proteomic databases

EPDiQ5T8I9
MaxQBiQ5T8I9
PaxDbiQ5T8I9
PeptideAtlasiQ5T8I9
PRIDEiQ5T8I9

PTM databases

iPTMnetiQ5T8I9
PhosphoSitePlusiQ5T8I9

Expressioni

Gene expression databases

BgeeiENSG00000162639
CleanExiHS_C1orf59
ExpressionAtlasiQ5T8I9 baseline and differential
GenevisibleiQ5T8I9 HS

Organism-specific databases

HPAiHPA028464
HPA028497

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi125261, 10 interactors
IntActiQ5T8I9, 2 interactors
MINTiQ5T8I9
STRINGi9606.ENSP00000359049

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 46Combined sources16
Beta strandi49 – 54Combined sources6
Helixi60 – 65Combined sources6
Beta strandi72 – 79Combined sources8
Helixi81 – 84Combined sources4
Helixi89 – 91Combined sources3
Helixi95 – 99Combined sources5
Beta strandi102 – 104Combined sources3
Beta strandi106 – 112Combined sources7
Helixi120 – 122Combined sources3
Beta strandi126 – 132Combined sources7
Helixi134 – 136Combined sources3
Helixi139 – 149Combined sources11
Turni150 – 153Combined sources4
Beta strandi156 – 164Combined sources9
Helixi165 – 167Combined sources3
Helixi178 – 180Combined sources3
Helixi187 – 201Combined sources15
Beta strandi203 – 210Combined sources8
Helixi218 – 220Combined sources3
Beta strandi223 – 231Combined sources9
Beta strandi250 – 258Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XCXX-ray2.84A14-262[»]
5WY0X-ray2.00A31-258[»]
ProteinModelPortaliQ5T8I9
SMRiQ5T8I9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1045 Eukaryota
ENOG410XSD6 LUCA
GeneTreeiENSGT00390000004798
HOGENOMiHOG000049068
HOVERGENiHBG097349
KOiK20798
PhylomeDBiQ5T8I9
TreeFamiTF315178

Family and domain databases

InterProiView protein in InterPro
IPR026610 Hen1
IPR029063 SAM-dependent_MTases
PANTHERiPTHR21404 PTHR21404, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q5T8I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENNLQCSS VVDGNFEEVP RETAIQFKPP LYRQRYQFVK NLVDQHEPKK
60 70 80 90 100
VADLGCGDTS LLRLLKVNPC IELLVGVDIN EDKLRWRGDS LAPFLGDFLK
110 120 130 140 150
PRDLNLTITL YHGSVVERDS RLLGFDLITC IELIEHLDSG DLARFPEVVF
160 170 180 190 200
GYLSPSMIVI STPNSEFNPL FPSVTLRDSD HKFEWTRMEF QTWALYVANR
210 220 230 240 250
YDYSVEFTGV GEPPAGAENV GYCTQIGIFR KNGGKATESC LSEQHDQHVY
260 270 280 290 300
KAVFTTSYPS LQQERFFKLV LVNEVSQQVE SLRVSHLPRR KEQAGERGDK
310 320 330 340 350
PKDIGGSKAP VPCFGPVFTE VEKAKIENSP TPFCVGDKFF VPLQRLLAYP
360 370 380 390
KLNRLCANEE MMRSVIADSI PLSSDGSAVV ADLRNYFDEQ FEF
Length:393
Mass (Da):44,525
Last modified:December 21, 2004 - v1
Checksum:i897A46664A44F6C5
GO

Sequence cautioni

The sequence CAI12930 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI12931 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti154S → F in BAB70852 (PubMed:14702039).Curated1
Sequence conflicti365V → A in BAB70852 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035017129T → A. Corresponds to variant dbSNP:rs9988420Ensembl.1
Natural variantiVAR_035018230R → Q. Corresponds to variant dbSNP:rs35974434Ensembl.1
Natural variantiVAR_035019361M → I1 PublicationCorresponds to variant dbSNP:rs17850887Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK055087 mRNA Translation: BAB70852.1
AL160171 Genomic DNA Translation: CAI12929.1
AL160171 Genomic DNA Translation: CAI12930.1 Sequence problems.
AL160171 Genomic DNA Translation: CAI12931.1 Sequence problems.
CH471122 Genomic DNA Translation: EAW56326.1
BC012198 mRNA Translation: AAH12198.1
BC088366 mRNA Translation: AAH88366.1
CCDSiCCDS787.1
RefSeqiNP_001096062.1, NM_001102592.1
NP_653185.2, NM_144584.2
UniGeneiHs.7962

Genome annotation databases

EnsembliENST00000370032; ENSP00000359049; ENSG00000162639
ENST00000402983; ENSP00000385655; ENSG00000162639
GeneIDi113802
KEGGihsa:113802
UCSCiuc001dvt.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHENMT_HUMAN
AccessioniPrimary (citable) accession number: Q5T8I9
Secondary accession number(s): A8MRR6
, B1AM16, B1AM17, Q96EJ7, Q96NN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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