Q5T848 (GP158_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor 158

Gene names

Name:	GPR158
Synonyms:	KIAA1136

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Orphan receptor.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.
Sequence caution	The sequence AAS18315.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein localization to plasma membrane Inferred from electronic annotation. Source: Compara regulation of G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 1215	1192	Probable G-protein coupled receptor 158		PRO_0000012969
Regions
	Topological domain	24 – 417	394	Extracellular Potential
	Transmembrane	418 – 438	21	Helical; Name=1; Potential
	Topological domain	439 – 451	13	Cytoplasmic Potential
	Transmembrane	452 – 472	21	Helical; Name=2; Potential
	Topological domain	473 – 483	11	Extracellular Potential
	Transmembrane	484 – 506	23	Helical; Name=3; Potential
	Topological domain	507 – 528	22	Cytoplasmic Potential
	Transmembrane	529 – 549	21	Helical; Name=4; Potential
	Topological domain	550 – 579	30	Extracellular Potential
	Transmembrane	580 – 600	21	Helical; Name=5; Potential
	Topological domain	601 – 613	13	Cytoplasmic Potential
	Transmembrane	614 – 634	21	Helical; Name=6; Potential
	Topological domain	635 – 643	9	Extracellular Potential
	Transmembrane	644 – 664	21	Helical; Name=7; Potential
	Topological domain	665 – 1215	551	Cytoplasmic Potential
Amino acid modifications
	Modified residue	904	1	Phosphoserine By similarity
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Cross-link	774		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
Natural variations
	Natural variant	425	1	A → G. Ref.1 Corresponds to variant rs2480345 [ dbSNP | Ensembl ].		VAR_049285
	Natural variant	1209	1	I → V. Corresponds to variant rs10828833 [ dbSNP | Ensembl ].		VAR_049286
Experimental info
	Sequence conflict	620 – 630	11	ELIISAIFHTI → SWIVNSMNSHF Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5T848 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: E0B565E573B3ADE1	FASTA	1,215	135,489
	10 20 30 40 50 60 MGAMAYPLLL CLLLAQLGLG AVGASRDPQG RPDSPRERTP KGKPHAQQPG RASASDSSAP 70 80 90 100 110 120 WSRSTDGTIL AQKLAEEVPM DVASYLYTGD SHQLKRANCS GRYELAGLPG KWPALASAHP 130 140 150 160 170 180 SLHRALDTLT HATNFLNVML QSNKSREQNL QDDLDWYQAL VWSLLEGEPS ISRAAITFST 190 200 210 220 230 240 DSLSAPAPQV FLQATREESR ILLQDLSSSA PHLANATLET EWFHGLRRKW RPHLHRRGPN 250 260 270 280 290 300 QGPRGLGHSW RRKDGLGGDK SHFKWSPPYL ECENGSYKPG WLVTLSSAIY GLQPNLVPEF 310 320 330 340 350 360 RGVMKVDINL QKVDIDQCSS DGWFSGTHKC HLNNSECMPI KGLGFVLGAY ECICKAGFYH 370 380 390 400 410 420 PGVLPVNNFR RRGPDQHISG STKDVSEEAY VCLPCREGCP FCADDSPCFV QEDKYLRLAI 430 440 450 460 470 480 ISFQALCMLL DFVSMLVVYH FRKAKSIRAS GLILLETILF GSLLLYFPVV ILYFEPSTFR 490 500 510 520 530 540 CILLRWARLL GFATVYGTVT LKLHRVLKVF LSRTAQRIPY MTGGRVMRML AVILLVVFWF 550 560 570 580 590 600 LIGWTSSVCQ NLEKQISLIG QGKTSDHLIF NMCLIDRWDY MTAVAEFLFL LWGVYLCYAV 610 620 630 640 650 660 RTVPSAFHEP RYMAVAVHNE LIISAIFHTI RFVLASRLQS DWMLMLYFAH THLTVTVTIG 670 680 690 700 710 720 LLLIPKFSHS SNNPRDDIAT EAYEDELDMG RSGSYLNSSI NSAWSEHSLD PEDIRDELKK 730 740 750 760 770 780 LYAQLEIYKR KKMITNNPHL QKKRCSKKGL GRSIMRRITE IPETVSRQCS KEDKEGADHG 790 800 810 820 830 840 TAKGTALIRK NPPESSGNTG KSKEETLKNR VFSLKKSHST YDHVRDQTEE SSSLPTESQE 850 860 870 880 890 900 EETTENSTLE SLSGKKLTQK LKEDSEAEST ESVPLVCKSA SAHNLSSEKK TGHPRTSMLQ 910 920 930 940 950 960 KSLSVIASAK EKTLGLAGKT QTAGVEERTK SQKPLPKDKE TNRNHSNSDN TETKDPAPQN 970 980 990 1000 1010 1020 SNPAEEPRKP QKSGIMKQQR VNPTTANSDL NPGTTQMKDN FDIGEVCPWE VYDLTPGPVP 1030 1040 1050 1060 1070 1080 SESKVQKHVS IVASEMEKNP TFSLKEKSHH KPKAAEVCQQ SNQKRIDKAE VCLWESQGQS 1090 1100 1110 1120 1130 1140 ILEDEKLLIS KTPVLPERAK EENGGQPRAA NVCAGQSEEL PPKAVASKTE NENLNQIGHQ 1150 1160 1170 1180 1190 1200 EKKTSSSEEN VRGSYNSSNN FQQPLTSRAE VCPWEFETPA QPNAGRSVAL PASSALSANK 1210 IAGPRKEEIW DSFKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete coding sequence of GPR158." Bonner T.I., Nagle J.W., Kauffman D. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-425. Tissue: Brain.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain." Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O. DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 620-1215. Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells." Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X. Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-774, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY528411 mRNA. Translation: AAS18315.1. Different initiation. AL161654, AL139821, AL355587 Genomic DNA. Translation: CAI16106.1. AL355587, AL139821, AL161654 Genomic DNA. Translation: CAI15725.1. AL139821, AL161654, AL355587 Genomic DNA. Translation: CAI16833.1. AB032962 mRNA. Translation: BAA86450.2.
IPI	IPI00412541.
RefSeq	NP_065803.2. NM_020752.2.
UniGene	Hs.499108.
3D structure databases
ProteinModelPortal	Q5T848.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5T848. 2 interactions.
STRING	9606.ENSP00000365529.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q5T848.
Polymorphism databases
DMDM	67461010.
Proteomic databases
PaxDb	Q5T848.
PRIDE	Q5T848.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000376351; ENSP00000365529; ENSG00000151025.
GeneID	57512.
KEGG	hsa:57512.
UCSC	uc001isj.3. human.
Organism-specific databases
CTD	57512.
GeneCards	GC10P025504.
HGNC	HGNC:23689. GPR158.
HPA	HPA013185.
MIM	614573. gene.
neXtProt	NX_Q5T848.
PharmGKB	PA134939018.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG328237.
HOGENOM	HOG000049235.
HOVERGEN	HBG080352.
InParanoid	Q5T848.
KO	K08469.
OMA	YKPGWLV.
OrthoDB	EOG4SBDX6.
Gene expression databases
Bgee	Q5T848.
CleanEx	HS_GPR158.
Genevestigator	Q5T848.
Family and domain databases
InterPro	IPR017978. GPCR_3_C. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. [Graphical view]
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57512.
NextBio	63874.
SOURCE	Search...

Entry information

Entry name

GP158_HUMAN

Accession

Primary (citable) accession number: Q5T848
Secondary accession number(s): Q6QR81, Q9ULT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.