Q5T6F0 (DCA12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DDB1- and CUL4-associated factor 12

Alternative name(s):
Centrosome-related protein TCC52
Testis cancer centrosome-related protein
WD repeat-containing protein 40A

Gene names

Name:	DCAF12
Synonyms:	KIAA1892, TCC52, WDR40A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.8
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DDB1. Ref.8
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome Ref.9.
Tissue specificity	Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis. Ref.9
Sequence similarities	Belongs to the WD repeat DCAF12 family. Contains 4 WD repeats.
Sequence caution	The sequence BAA91106.1 differs from that shown. Reason: Frameshift at position 27. The sequence BAB67785.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI15300.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15302.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Domain	Repeat WD repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination Inferred by curator Ref.8. Source: UniProtKB
Cellular component	CUL4 RING ubiquitin ligase complex Inferred from direct assay Ref.8. Source: UniProtKB centrosome Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 453

453

DDB1- and CUL4-associated factor 12

PRO_0000306840

Regions

Repeat

138 – 178

41

WD 1

Repeat

182 – 220

39

WD 2

Repeat

250 – 289

40

WD 3

Repeat

338 – 375

38

WD 4

Natural variations

Natural variant

131

1

R → Q. Ref.6
Corresponds to variant rs11557154 [ dbSNP | Ensembl ].

VAR_035322

Experimental info

Mutagenesis

368

1

R → A: Reduces association with DDB1. Ref.8

Sequence conflict

149

1

N → H in BAA91106. Ref.3

Secondary structure

1

.

.

.

453

Helix Strand Turn

Details...

Helix

47 – 55

9

Sequences

	Sequence		Length	Mass (Da)
	Q5T6F0 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 07F3738BA6BB5355	FASTA	453	50,517
	10 20 30 40 50 60 MARKVVSRKR KAPASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL KNREVRLQNE 70 80 90 100 110 120 TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH RQVVCGTKCN TLFVVDVQTS 130 140 150 160 170 180 QITKIPILKD REPGGVTQQG CGIHAIELNP SRTLLATGGD NPNSLAIYRL PTLDPVCVGD 190 200 210 220 230 240 DGHKDWIFSI AWISDTMAVS GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL 250 260 270 280 290 300 KDIPKEDTNP DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV 310 320 330 340 350 360 CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE HIITVGTGQG 370 380 390 400 410 420 SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW LNHDETWRNY FSDIDFFPNA 430 440 450 VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG LWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	He D., Li S., Hu X., Luo C., Ceng C. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:179-187(2001) [PubMed: 11572484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hepatoma and Trachea.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-131. Tissue: Lymph and PNS.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-453. Tissue: Testis.
[8]	"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006) [PubMed: 16949367] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDB1, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF ARG-368.
[9]	"Novel centrosome protein, TCC52, is a cancer-testis antigen." Li S., Hu X., Cui S., He D. Cancer Sci. 99:2274-2279(2008) [PubMed: 18957058] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ518909 mRNA. Translation: ABG24246.1.
AB067479 mRNA. Translation: BAB67785.1. Different initiation.
AK000354 mRNA. Translation: BAA91106.1. Frameshift.
AK292935 mRNA. Translation: BAF85624.1.
AL354989 Genomic DNA. Translation: CAI15300.1. Sequence problems.
AL354989 Genomic DNA. Translation: CAI15301.1.
AL354989 Genomic DNA. Translation: CAI15302.1. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58472.1.
CH471071 Genomic DNA. Translation: EAW58473.1.
BC008893 mRNA. Translation: AAH08893.2.
BC063823 mRNA. Translation: AAH63823.1.
AL117646 mRNA. Translation: CAB56028.2.

IPI

IPI00411753.

PIR

T17338.

RefSeq

NP_056212.1. NM_015397.3.

UniGene

Hs.493750.
Hs.726117.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I7P	X-ray	3.00	B	45-57	[»]

ProteinModelPortal

Q5T6F0.

SMR

Q5T6F0. Positions 143-169.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48760N.

IntAct

Q5T6F0. 2 interactions.

STRING

Q5T6F0.

PTM databases

PhosphoSite

Q5T6F0.

Polymorphism databases

DMDM

74756352.

Proteomic databases

PRIDE

Q5T6F0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361264; ENSP00000355114; ENSG00000198876.

GeneID

25853.

KEGG

hsa:25853.

UCSC

uc003ztt.1. human.

Organism-specific databases

CTD

25853.

GeneCards

GC09M034079.

H-InvDB

HIX0007987.
HIX0028448.

HGNC

HGNC:19911. DCAF12.

HPA

HPA016596.

neXtProt

NX_Q5T6F0.

PharmGKB

PA134885697.
PA165585735.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG14161.

GeneTree

ENSGT00390000001132.

HOVERGEN

HBG055605.

InParanoid

Q5T6F0.

OMA

DETWRSY.

OrthoDB

EOG4PK27V.

PhylomeDB

Q5T6F0.

Gene expression databases

ArrayExpress

Q5T6F0.

Bgee

Q5T6F0.

CleanEx

HS_WDR40A.

Genevestigator

Q5T6F0.

Family and domain databases

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K11803.

Pfam

PF00400. WD40. 1 hit.
[Graphical view]

SMART

SM00320. WD40. 4 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

47204.

Entry information

Entry name

DCA12_HUMAN

Accession

Primary (citable) accession number: Q5T6F0
Secondary accession number(s): A8KA70 , D3DRL6, Q5T6E9, Q5T6F1, Q6P3V0, Q7L4F8, Q96PZ5, Q9NXA9, Q9UFJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 21, 2004
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.