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Protein

DDB1- and CUL4-associated factor 12

Gene

DCAF12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:G66-31879-MONOMER.
SignaLinkiQ5T6F0.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 12
Alternative name(s):
Centrosome-related protein TCC52
Testis cancer centrosome-related protein
WD repeat-containing protein 40A
Gene namesi
Name:DCAF12
Synonyms:KIAA1892, TCC52, WDR40A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:19911. DCAF12.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi368R → A: Reduces association with DDB1. 1 Publication1

Organism-specific databases

DisGeNETi25853.
OpenTargetsiENSG00000198876.
PharmGKBiPA165585735.

Polymorphism and mutation databases

BioMutaiDCAF12.
DMDMi74756352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068401 – 453DDB1- and CUL4-associated factor 12Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5T6F0.
MaxQBiQ5T6F0.
PaxDbiQ5T6F0.
PeptideAtlasiQ5T6F0.
PRIDEiQ5T6F0.

PTM databases

iPTMnetiQ5T6F0.
PhosphoSitePlusiQ5T6F0.

Expressioni

Tissue specificityi

Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis.1 Publication

Gene expression databases

BgeeiENSG00000198876.
CleanExiHS_WDR40A.
ExpressionAtlasiQ5T6F0. baseline and differential.
GenevisibleiQ5T6F0. HS.

Organism-specific databases

HPAiHPA016596.

Interactioni

Subunit structurei

Interacts with DDB1.1 Publication

Protein-protein interaction databases

BioGridi117377. 19 interactors.
DIPiDIP-48760N.
IntActiQ5T6F0. 2 interactors.
STRINGi9606.ENSP00000355114.

Structurei

Secondary structure

1453
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 55Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I7PX-ray3.00B45-57[»]
ProteinModelPortaliQ5T6F0.
SMRiQ5T6F0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5T6F0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati81 – 122WD 1Add BLAST42
Repeati123 – 175WD 2Add BLAST53
Repeati176 – 242WD 3Add BLAST67
Repeati243 – 286WD 4Add BLAST44
Repeati287 – 331WD 5Add BLAST45
Repeati332 – 366WD 6Add BLAST35

Sequence similaritiesi

Belongs to the WD repeat DCAF12 family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IGRG. Eukaryota.
ENOG410XTH6. LUCA.
GeneTreeiENSGT00390000001132.
HOVERGENiHBG055605.
InParanoidiQ5T6F0.
KOiK11803.
OMAiYNCKVRA.
OrthoDBiEOG091G05P2.
PhylomeDBiQ5T6F0.
TreeFamiTF323731.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5T6F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKVVSRKR KAPASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL
60 70 80 90 100
KNREVRLQNE TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH
110 120 130 140 150
RQVVCGTKCN TLFVVDVQTS QITKIPILKD REPGGVTQQG CGIHAIELNP
160 170 180 190 200
SRTLLATGGD NPNSLAIYRL PTLDPVCVGD DGHKDWIFSI AWISDTMAVS
210 220 230 240 250
GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL KDIPKEDTNP
260 270 280 290 300
DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV
310 320 330 340 350
CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE
360 370 380 390 400
HIITVGTGQG SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW
410 420 430 440 450
LNHDETWRNY FSDIDFFPNA VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG

LWS
Length:453
Mass (Da):50,517
Last modified:December 21, 2004 - v1
Checksum:i07F3738BA6BB5355
GO

Sequence cautioni

The sequence BAA91106 differs from that shown. Reason: Frameshift at position 27.Curated
The sequence BAB67785 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI15300 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI15302 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149N → H in BAA91106 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035322131R → Q.1 PublicationCorresponds to variant rs11557154dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ518909 mRNA. Translation: ABG24246.1.
AB067479 mRNA. Translation: BAB67785.1. Different initiation.
AK000354 mRNA. Translation: BAA91106.1. Frameshift.
AK292935 mRNA. Translation: BAF85624.1.
AL354989 Genomic DNA. Translation: CAI15300.1. Sequence problems.
AL354989 Genomic DNA. Translation: CAI15301.1.
AL354989 Genomic DNA. Translation: CAI15302.1. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58472.1.
CH471071 Genomic DNA. Translation: EAW58473.1.
BC008893 mRNA. Translation: AAH08893.2.
BC063823 mRNA. Translation: AAH63823.1.
AL117646 mRNA. Translation: CAB56028.2.
CCDSiCCDS6549.1.
PIRiT17338.
RefSeqiNP_056212.1. NM_015397.3.
XP_011516145.1. XM_011517843.1.
UniGeneiHs.493750.
Hs.741270.

Genome annotation databases

EnsembliENST00000361264; ENSP00000355114; ENSG00000198876.
GeneIDi25853.
KEGGihsa:25853.
UCSCiuc003ztt.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ518909 mRNA. Translation: ABG24246.1.
AB067479 mRNA. Translation: BAB67785.1. Different initiation.
AK000354 mRNA. Translation: BAA91106.1. Frameshift.
AK292935 mRNA. Translation: BAF85624.1.
AL354989 Genomic DNA. Translation: CAI15300.1. Sequence problems.
AL354989 Genomic DNA. Translation: CAI15301.1.
AL354989 Genomic DNA. Translation: CAI15302.1. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58472.1.
CH471071 Genomic DNA. Translation: EAW58473.1.
BC008893 mRNA. Translation: AAH08893.2.
BC063823 mRNA. Translation: AAH63823.1.
AL117646 mRNA. Translation: CAB56028.2.
CCDSiCCDS6549.1.
PIRiT17338.
RefSeqiNP_056212.1. NM_015397.3.
XP_011516145.1. XM_011517843.1.
UniGeneiHs.493750.
Hs.741270.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I7PX-ray3.00B45-57[»]
ProteinModelPortaliQ5T6F0.
SMRiQ5T6F0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117377. 19 interactors.
DIPiDIP-48760N.
IntActiQ5T6F0. 2 interactors.
STRINGi9606.ENSP00000355114.

PTM databases

iPTMnetiQ5T6F0.
PhosphoSitePlusiQ5T6F0.

Polymorphism and mutation databases

BioMutaiDCAF12.
DMDMi74756352.

Proteomic databases

EPDiQ5T6F0.
MaxQBiQ5T6F0.
PaxDbiQ5T6F0.
PeptideAtlasiQ5T6F0.
PRIDEiQ5T6F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361264; ENSP00000355114; ENSG00000198876.
GeneIDi25853.
KEGGihsa:25853.
UCSCiuc003ztt.3. human.

Organism-specific databases

CTDi25853.
DisGeNETi25853.
GeneCardsiDCAF12.
H-InvDBHIX0169338.
HGNCiHGNC:19911. DCAF12.
HPAiHPA016596.
neXtProtiNX_Q5T6F0.
OpenTargetsiENSG00000198876.
PharmGKBiPA165585735.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGRG. Eukaryota.
ENOG410XTH6. LUCA.
GeneTreeiENSGT00390000001132.
HOVERGENiHBG055605.
InParanoidiQ5T6F0.
KOiK11803.
OMAiYNCKVRA.
OrthoDBiEOG091G05P2.
PhylomeDBiQ5T6F0.
TreeFamiTF323731.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:G66-31879-MONOMER.
SignaLinkiQ5T6F0.

Miscellaneous databases

EvolutionaryTraceiQ5T6F0.
GenomeRNAii25853.
PROiQ5T6F0.

Gene expression databases

BgeeiENSG00000198876.
CleanExiHS_WDR40A.
ExpressionAtlasiQ5T6F0. baseline and differential.
GenevisibleiQ5T6F0. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCA12_HUMAN
AccessioniPrimary (citable) accession number: Q5T6F0
Secondary accession number(s): A8KA70
, D3DRL6, Q5T6E9, Q5T6F1, Q6P3V0, Q7L4F8, Q96PZ5, Q9NXA9, Q9UFJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.