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Protein

DDB1- and CUL4-associated factor 12

Gene

DCAF12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ5T6F0.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 12
Alternative name(s):
Centrosome-related protein TCC52
Testis cancer centrosome-related protein
WD repeat-containing protein 40A
Gene namesi
Name:DCAF12
Synonyms:KIAA1892, TCC52, WDR40A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:19911. DCAF12.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi368 – 3681R → A: Reduces association with DDB1. 1 Publication

Organism-specific databases

PharmGKBiPA165585735.

Polymorphism and mutation databases

BioMutaiDCAF12.
DMDMi74756352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453DDB1- and CUL4-associated factor 12PRO_0000306840Add
BLAST

Proteomic databases

EPDiQ5T6F0.
MaxQBiQ5T6F0.
PaxDbiQ5T6F0.
PRIDEiQ5T6F0.

PTM databases

iPTMnetiQ5T6F0.
PhosphoSiteiQ5T6F0.

Expressioni

Tissue specificityi

Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis.1 Publication

Gene expression databases

BgeeiQ5T6F0.
CleanExiHS_WDR40A.
ExpressionAtlasiQ5T6F0. baseline and differential.
GenevisibleiQ5T6F0. HS.

Organism-specific databases

HPAiHPA016596.

Interactioni

Subunit structurei

Interacts with DDB1.1 Publication

Protein-protein interaction databases

BioGridi117377. 19 interactions.
DIPiDIP-48760N.
IntActiQ5T6F0. 2 interactions.
STRINGi9606.ENSP00000355114.

Structurei

Secondary structure

1
453
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi47 – 559Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I7PX-ray3.00B45-57[»]
ProteinModelPortaliQ5T6F0.
SMRiQ5T6F0. Positions 254-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5T6F0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati81 – 12242WD 1Add
BLAST
Repeati123 – 17553WD 2Add
BLAST
Repeati176 – 24267WD 3Add
BLAST
Repeati243 – 28644WD 4Add
BLAST
Repeati287 – 33145WD 5Add
BLAST
Repeati332 – 36635WD 6Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat DCAF12 family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IGRG. Eukaryota.
ENOG410XTH6. LUCA.
GeneTreeiENSGT00390000001132.
HOVERGENiHBG055605.
InParanoidiQ5T6F0.
KOiK11803.
OMAiYNCKVRA.
OrthoDBiEOG73NG34.
PhylomeDBiQ5T6F0.
TreeFamiTF323731.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5T6F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKVVSRKR KAPASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL
60 70 80 90 100
KNREVRLQNE TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH
110 120 130 140 150
RQVVCGTKCN TLFVVDVQTS QITKIPILKD REPGGVTQQG CGIHAIELNP
160 170 180 190 200
SRTLLATGGD NPNSLAIYRL PTLDPVCVGD DGHKDWIFSI AWISDTMAVS
210 220 230 240 250
GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL KDIPKEDTNP
260 270 280 290 300
DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV
310 320 330 340 350
CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE
360 370 380 390 400
HIITVGTGQG SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW
410 420 430 440 450
LNHDETWRNY FSDIDFFPNA VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG

LWS
Length:453
Mass (Da):50,517
Last modified:December 21, 2004 - v1
Checksum:i07F3738BA6BB5355
GO

Sequence cautioni

The sequence BAA91106.1 differs from that shown. Reason: Frameshift at position 27. Curated
The sequence BAB67785.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI15300.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAI15302.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491N → H in BAA91106 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti131 – 1311R → Q.1 Publication
Corresponds to variant rs11557154 [ dbSNP | Ensembl ].
VAR_035322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ518909 mRNA. Translation: ABG24246.1.
AB067479 mRNA. Translation: BAB67785.1. Different initiation.
AK000354 mRNA. Translation: BAA91106.1. Frameshift.
AK292935 mRNA. Translation: BAF85624.1.
AL354989 Genomic DNA. Translation: CAI15300.1. Sequence problems.
AL354989 Genomic DNA. Translation: CAI15301.1.
AL354989 Genomic DNA. Translation: CAI15302.1. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58472.1.
CH471071 Genomic DNA. Translation: EAW58473.1.
BC008893 mRNA. Translation: AAH08893.2.
BC063823 mRNA. Translation: AAH63823.1.
AL117646 mRNA. Translation: CAB56028.2.
CCDSiCCDS6549.1.
PIRiT17338.
RefSeqiNP_056212.1. NM_015397.3.
XP_011516145.1. XM_011517843.1.
UniGeneiHs.493750.
Hs.741270.

Genome annotation databases

EnsembliENST00000361264; ENSP00000355114; ENSG00000198876.
GeneIDi25853.
KEGGihsa:25853.
UCSCiuc003ztt.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ518909 mRNA. Translation: ABG24246.1.
AB067479 mRNA. Translation: BAB67785.1. Different initiation.
AK000354 mRNA. Translation: BAA91106.1. Frameshift.
AK292935 mRNA. Translation: BAF85624.1.
AL354989 Genomic DNA. Translation: CAI15300.1. Sequence problems.
AL354989 Genomic DNA. Translation: CAI15301.1.
AL354989 Genomic DNA. Translation: CAI15302.1. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58472.1.
CH471071 Genomic DNA. Translation: EAW58473.1.
BC008893 mRNA. Translation: AAH08893.2.
BC063823 mRNA. Translation: AAH63823.1.
AL117646 mRNA. Translation: CAB56028.2.
CCDSiCCDS6549.1.
PIRiT17338.
RefSeqiNP_056212.1. NM_015397.3.
XP_011516145.1. XM_011517843.1.
UniGeneiHs.493750.
Hs.741270.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I7PX-ray3.00B45-57[»]
ProteinModelPortaliQ5T6F0.
SMRiQ5T6F0. Positions 254-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117377. 19 interactions.
DIPiDIP-48760N.
IntActiQ5T6F0. 2 interactions.
STRINGi9606.ENSP00000355114.

PTM databases

iPTMnetiQ5T6F0.
PhosphoSiteiQ5T6F0.

Polymorphism and mutation databases

BioMutaiDCAF12.
DMDMi74756352.

Proteomic databases

EPDiQ5T6F0.
MaxQBiQ5T6F0.
PaxDbiQ5T6F0.
PRIDEiQ5T6F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361264; ENSP00000355114; ENSG00000198876.
GeneIDi25853.
KEGGihsa:25853.
UCSCiuc003ztt.3. human.

Organism-specific databases

CTDi25853.
GeneCardsiDCAF12.
H-InvDBHIX0169338.
HGNCiHGNC:19911. DCAF12.
HPAiHPA016596.
neXtProtiNX_Q5T6F0.
PharmGKBiPA165585735.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGRG. Eukaryota.
ENOG410XTH6. LUCA.
GeneTreeiENSGT00390000001132.
HOVERGENiHBG055605.
InParanoidiQ5T6F0.
KOiK11803.
OMAiYNCKVRA.
OrthoDBiEOG73NG34.
PhylomeDBiQ5T6F0.
TreeFamiTF323731.

Enzyme and pathway databases

UniPathwayiUPA00143.
SignaLinkiQ5T6F0.

Miscellaneous databases

EvolutionaryTraceiQ5T6F0.
GenomeRNAii25853.
NextBioi47204.
PROiQ5T6F0.

Gene expression databases

BgeeiQ5T6F0.
CleanExiHS_WDR40A.
ExpressionAtlasiQ5T6F0. baseline and differential.
GenevisibleiQ5T6F0. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. He D., Li S., Hu X., Luo C., Ceng C.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:179-187(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Hepatoma and Trachea.
  4. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-131.
    Tissue: Lymph and PNS.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-453.
    Tissue: Testis.
  8. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."
    Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C.
    Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DDB1, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF ARG-368.
  9. "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery."
    Angers S., Li T., Yi X., MacCoss M.J., Moon R.T., Zheng N.
    Nature 443:590-593(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Novel centrosome protein, TCC52, is a cancer-testis antigen."
    Li S., Hu X., Cui S., He D.
    Cancer Sci. 99:2274-2279(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDCA12_HUMAN
AccessioniPrimary (citable) accession number: Q5T6F0
Secondary accession number(s): A8KA70
, D3DRL6, Q5T6E9, Q5T6F1, Q6P3V0, Q7L4F8, Q96PZ5, Q9NXA9, Q9UFJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: December 21, 2004
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.