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Protein

Calmodulin-regulated spectrin-associated protein 1

Gene

CAMSAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19508979, PubMed:21834987, PubMed:24486153, PubMed:24706919, PubMed:24117850). Specifically recognizes growing microtubule minus-ends and stabilizes microtubules (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation (PubMed:24486153, PubMed:24706919). Through interaction with spectrin may regulate neurite outgrowth (PubMed:24117850).5 Publications

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • microtubule minus-end binding Source: UniProtKB
  • spectrin binding Source: UniProtKB

GO - Biological processi

  • cytoskeleton organization Source: UniProtKB
  • microtubule cytoskeleton organization Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • regulation of cell morphogenesis Source: UniProtKB
  • regulation of microtubule polymerization Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-regulated spectrin-associated protein 1
Gene namesi
Name:CAMSAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000130559.18
HGNCiHGNC:19946 CAMSAP1
MIMi613774 gene
neXtProtiNX_Q5T5Y3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi885 – 888LEEK → AAAA: Loss of interaction with SPTBN1. 1 Publication4

Organism-specific databases

DisGeNETi157922
OpenTargetsiENSG00000130559
PharmGKBiPA134866541

Polymorphism and mutation databases

BioMutaiCAMSAP1
DMDMi166991445

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168281 – 1602Calmodulin-regulated spectrin-associated protein 1Add BLAST1602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei217PhosphoserineCombined sources1
Modified residuei371PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei512PhosphothreonineCombined sources1
Modified residuei563PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei589PhosphoserineCombined sources1
Modified residuei629PhosphoserineCombined sources1
Modified residuei722PhosphoserineCombined sources1
Modified residuei728PhosphoserineBy similarity1
Modified residuei738PhosphoserineBy similarity1
Modified residuei740PhosphoserineBy similarity1
Modified residuei1080PhosphoserineCombined sources1
Modified residuei1398PhosphoserineCombined sources1
Modified residuei1427PhosphoserineBy similarity1
Modified residuei1537PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5T5Y3
MaxQBiQ5T5Y3
PaxDbiQ5T5Y3
PeptideAtlasiQ5T5Y3
PRIDEiQ5T5Y3

PTM databases

iPTMnetiQ5T5Y3
PhosphoSitePlusiQ5T5Y3

Expressioni

Gene expression databases

BgeeiENSG00000130559
CleanExiHS_CAMSAP1
GenevisibleiQ5T5Y3 HS

Organism-specific databases

HPAiHPA020527
HPA024161

Interactioni

Subunit structurei

Interacts with spectrin via SPTBN1; the interaction is direct (PubMed:24117850). Interacts with calmodulin; calcium-dependent it prevents interaction with spectrin (PubMed:24117850).1 Publication

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB
  • microtubule binding Source: UniProtKB
  • microtubule minus-end binding Source: UniProtKB
  • spectrin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127632, 24 interactors
IntActiQ5T5Y3, 30 interactors
MINTiQ5T5Y3
STRINGi9606.ENSP00000374183

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5M54electron microscopy8.00C1473-1589[»]
5M5Celectron microscopy4.80C1472-1589[»]
ProteinModelPortaliQ5T5Y3
SMRiQ5T5Y3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini216 – 331Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST116
Domaini1463 – 1597CKKPROSITE-ProRule annotationAdd BLAST135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni871 – 892Sufficient for interaction with SPTBN11 PublicationAdd BLAST22
Regioni903 – 922Sufficient for interaction with calmodulinAdd BLAST20

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili873 – 909Sequence analysisAdd BLAST37
Coiled coili1016 – 1048Sequence analysisAdd BLAST33
Coiled coili1291 – 1343Sequence analysisAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi536 – 541Poly-Glu6
Compositional biasi1210 – 1213Poly-Ser4

Domaini

The CKK domain binds microtubules.PROSITE-ProRule annotation1 Publication

Sequence similaritiesi

Belongs to the CAMSAP1 family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3654 Eukaryota
ENOG4111D0B LUCA
GeneTreeiENSGT00390000010026
HOVERGENiHBG107572
InParanoidiQ5T5Y3
KOiK17493
OMAiWICAKAY
OrthoDBiEOG091G00FI
PhylomeDBiQ5T5Y3
TreeFamiTF315529

Family and domain databases

Gene3Di1.10.418.10, 1 hit
3.10.20.360, 1 hit
InterProiView protein in InterPro
IPR032940 CAMSAP
IPR031372 CAMSAP_CC1
IPR022613 CAMSAP_CH
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR038209 CKK_dom_sf
IPR014797 CKK_domain
IPR011033 PRC_barrel-like_sf
PANTHERiPTHR21595 PTHR21595, 1 hit
PfamiView protein in Pfam
PF17095 CAMSAP_CC1, 1 hit
PF11971 CAMSAP_CH, 1 hit
PF08683 CAMSAP_CKK, 1 hit
SMARTiView protein in SMART
SM01051 CAMSAP_CKK, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF50346 SSF50346, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51508 CKK, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T5Y3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVDASGRAAA EGWRKMEAPP DGAADLVPLD RYDAARAKIA ANLQWICAKA
60 70 80 90 100
YGRDNIPEDL RDPFYVDQYE QEHIKPPVIK LLLSSELYCR VCSLILKGDQ
110 120 130 140 150
VAALQGHQSV IQALSRKGIY VMESDDTPVT ESDLSRAPIK MSAHMAMVDA
160 170 180 190 200
LMMAYTVEMI SIEKVVASVK RFSTFSASKE LPYDLEDAMV FWINKVNLKM
210 220 230 240 250
REITEKEVKL KQQLLESPAH QKVRYRREHL SARQSPYFPL LEDLMRDGSD
260 270 280 290 300
GAALLAVIHY YCPEQMKLDD ICLKEVTSMA DSLYNIRLLR EFSNEYLNKC
310 320 330 340 350
FYLTLEDMLY APLVLKPNVM VFIAELFWWF ENVKPDFVQP RDVQELKDAK
360 370 380 390 400
TVLHQKSSRP PVPISNATKR SFLGSPAAGT LAELQPPVQL PAEGCHRHYL
410 420 430 440 450
HPEEPEYLGK GTAAFSPSHP LLPLRQKQQK SIQGEDIPDQ RHRSNSLTRV
460 470 480 490 500
DGQPRGAAIA WPEKKTRPAS QPTPFALHHA ASCEVDPSSG DSISLARSIS
510 520 530 540 550
KDSLASNIVN LTPQNQPHPT ATKSHGKSLL SNVSIEDEEE ELVAIVRADV
560 570 580 590 600
VPQQADPEFP RASPRALGLT ANARSPQGQL DTSESKPDSF FLEPLMPAVL
610 620 630 640 650
KPAKEKQVIT KEDERGEGRP RSIVSRRPSE GPQPLVRRKM TGSRDLNRTF
660 670 680 690 700
TPIPCSEFPM GIDPTETGPL SVETAGEVCG GPLALGGFDP FPQGPSTDGF
710 720 730 740 750
FLHVGRADED TEGRLYVSCS KSPNSHDSEP WTLLRQDSDS DVVDIEEAEH
760 770 780 790 800
DFMGEAHPVV FSRYIGEEES AKLQEDMKVK EHEDKDDASG RSSPCLSTAS
810 820 830 840 850
QMSSVSMASG SVKMTSFAER KLQRLNSCET KSSTSSSQKT TPDASESCPA
860 870 880 890 900
PLTTWRQKRE QSPSQHGKDP ASLLASELVQ LHMQLEEKRR AIEAQKKKME
910 920 930 940 950
ALSARQRLKL GKAAFLHVVK KGKAEAAPPL RPEHFAKEYS QHNGEDCGDA
960 970 980 990 1000
VSKTEDFLVK EEQREELLHE PQDVDKESLA FAQQHKAKDP VALHELERNK
1010 1020 1030 1040 1050
VISAALLEDT VGEVVDVNEC DLSIEKLNET ISTLQQAILK ISQQQEQLLM
1060 1070 1080 1090 1100
KSPTVPVPGS KNNSQDHKVK APVHFVEPLS PTGVAGHRKA PRLGQGRNSR
1110 1120 1130 1140 1150
SGRPAELKVP KDRPQGSSRS KTPTPSVETL PHLRPFPASS HPRTPTDPGL
1160 1170 1180 1190 1200
DSALEPSGDP HGKCLFDSYR LHDESNQRTL TLSSSKDANI LSEQMSLKEV
1210 1220 1230 1240 1250
LDASVKEVGS SSSDVSGKES VPVEEPLRSR ASLIEVDLSD LKAPDEDGEL
1260 1270 1280 1290 1300
VSLDGSADLV SEGDQKPGVG FFFKDEQKAE DELAKKRAAF LLKQQRKAEE
1310 1320 1330 1340 1350
ARVRKQQLEA EVELKRDEAR RKAEEDRVRK EEEKARRELI KQEYLRRKQQ
1360 1370 1380 1390 1400
QILEEQGLGK PKSKPKKPRP KSVHREESCS DSGTKCSSTP DNLSRTQSGS
1410 1420 1430 1440 1450
SLSLASAATT EPESVHSGGT PSQRVESMEA LPILSRNPSR STDRDWETAS
1460 1470 1480 1490 1500
AASSLASVAE YTGPKLFKEP SSKSNKPIIH NAISHCCLAG KVNEPHKNSI
1510 1520 1530 1540 1550
LEELEKCDAN HYIILFRDAG CQFRALYCYY PDTEEIYKLT GTGPKNITKK
1560 1570 1580 1590 1600
MIDKLYKYSS DRKQFNLIPA KTMSVSVDAL TIHNHLWQPK RPAVPKKAQT

RK
Length:1,602
Mass (Da):177,972
Last modified:February 20, 2007 - v2
Checksum:i184823C2A151F8A7
GO
Isoform 2 (identifier: Q5T5Y3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-278: Missing.

Note: No experimental confirmation available.
Show »
Length:1,324
Mass (Da):146,546
Checksum:iFA81C36B97C9F87F
GO
Isoform 3 (identifier: Q5T5Y3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-222: K → KSPSKWYWKLVP

Note: No experimental confirmation available.
Show »
Length:1,613
Mass (Da):179,345
Checksum:i2B088369A51DB53D
GO

Sequence cautioni

The sequence AAI30581 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti823Q → H in AAH12778 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038398476A → V. Corresponds to variant dbSNP:rs35639321Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0308001 – 278Missing in isoform 2. 1 PublicationAdd BLAST278
Alternative sequenceiVSP_030801222K → KSPSKWYWKLVP in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ519841 mRNA Translation: CAD58627.1
AL158822 Genomic DNA No translation available.
AL353636 Genomic DNA No translation available.
AL355574 Genomic DNA No translation available.
BC010646 mRNA Translation: AAH10646.1
BC012778 mRNA Translation: AAH12778.2
BC130580 mRNA Translation: AAI30581.1 Different initiation.
AL117634 mRNA Translation: CAB56024.2
AL834528 mRNA Translation: CAD39184.1
CCDSiCCDS35176.2 [Q5T5Y3-1]
PIRiT17334
RefSeqiNP_056262.3, NM_015447.3 [Q5T5Y3-1]
XP_005263453.1, XM_005263396.3 [Q5T5Y3-3]
XP_005263454.1, XM_005263397.1 [Q5T5Y3-2]
UniGeneiHs.522493

Genome annotation databases

EnsembliENST00000312405; ENSP00000312463; ENSG00000130559 [Q5T5Y3-2]
ENST00000389532; ENSP00000374183; ENSG00000130559 [Q5T5Y3-1]
ENST00000409386; ENSP00000386420; ENSG00000130559 [Q5T5Y3-3]
GeneIDi157922
KEGGihsa:157922
UCSCiuc004cgr.5 human [Q5T5Y3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCAMP1_HUMAN
AccessioniPrimary (citable) accession number: Q5T5Y3
Secondary accession number(s): A1L4L2
, B2REB2, B2REB3, Q70W33, Q8NCY0, Q96E80, Q96FM3, Q9UFJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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