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Q5T5C0 (STXB5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-binding protein 5
Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1
Gene names
Name:STXBP5
Synonyms:LLGL3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1151 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane. Competes with STXBP1 for STX1 binding By similarity.

Subunit structure

Interacts with STX1A and STX1B via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1. Part of a complex that contains STXBP5, STX4A and SNAP23 By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein By similarity. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle By similarity. Cell junctionsynapse By similarity. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles By similarity.

Sequence similarities

Belongs to the WD repeat L(2)GL family.

Contains 1 v-SNARE coiled-coil homology domain.

Contains 14 WD repeats.

Sequence caution

The sequence BAC03475.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5T5C0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5T5C0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     716-751: Missing.
Isoform 3 (identifier: Q5T5C0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     716-768: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11511151Syntaxin-binding protein 5
PRO_0000051244

Regions

Repeat61 – 9434WD 1
Repeat101 – 14040WD 2
Repeat145 – 18137WD 3
Repeat200 – 23435WD 4
Repeat240 – 27233WD 5
Repeat294 – 33643WD 6
Repeat344 – 37835WD 7
Repeat400 – 47778WD 8
Repeat505 – 619115WD 9
Repeat633 – 69563WD 10
Repeat794 – 85158WD 11
Repeat860 – 93475WD 12
Repeat939 – 98345WD 13
Repeat997 – 102024WD 14
Domain1086 – 114661v-SNARE coiled-coil homology
Compositional bias22 – 276Poly-Gln
Compositional bias584 – 5907Poly-Ser

Amino acid modifications

Modified residue7231Phosphoserine; by PKA By similarity
Modified residue7591Phosphoserine Ref.5
Modified residue7621Phosphothreonine Ref.4
Modified residue7821Phosphoserine By similarity
Modified residue7851Phosphoserine Ref.6

Natural variations

Alternative sequence716 – 76853Missing in isoform 3.
VSP_016204
Alternative sequence716 – 75136Missing in isoform 2.
VSP_016205
Natural variant4361N → S.
Corresponds to variant rs1039084 [ dbSNP | Ensembl ].
VAR_035235

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 247083A17DEA4811

FASTA1,151127,573
        10         20         30         40         50         60 
MRKFNIRKVL DGLTAGSSSA SQQQQQQHPP GNREPEIQET LQSEHFQLCK TVRHGFPYQP 

        70         80         90        100        110        120 
SALAFDPVQK ILAVGTQTGA LRLFGRPGVE CYCQHDSGAA VIQLQFLINE GALVSALADD 

       130        140        150        160        170        180 
TLHLWNLRQK RPAILHSLKF CRERVTFCHL PFQSKWLYVG TERGNIHIVN VESFTLSGYV 

       190        200        210        220        230        240 
IMWNKAIELS SKSHPGPVVH ISDNPMDEGK LLIGFESGTV VLWDLKSKKA DYRYTYDEAI 

       250        260        270        280        290        300 
HSVAWHHEGK QFICSHSDGT LTIWNVRSPA KPVQTITPHG KQLKDGKKPE PCKPILKVEF 

       310        320        330        340        350        360 
KTTRSGEPFI ILSGGLSYDT VGRRPCLTVM HGKSTAVLEM DYSIVDFLTL CETPYPNDFQ 

       370        380        390        400        410        420 
EPYAVVVLLE KDLVLIDLAQ NGYPIFENPY PLSIHESPVT CCEYFADCPV DLIPALYSVG 

       430        440        450        460        470        480 
ARQKRQGYSK KEWPINGGNW GLGAQSYPEI IITGHADGSV KFWDASAITL QVLYKLKTSK 

       490        500        510        520        530        540 
VFEKSRNKDD RPNTDIVDED PYAIQIISWC PESRMLCIAG VSAHVIIYRF SKQEVITEVI 

       550        560        570        580        590        600 
PMLEVRLLYE INDVETPEGE QPPPLPTPVG GSNPQPIPPQ SHPSTSSSSS DGLRDNVPCL 

       610        620        630        640        650        660 
KVKNSPLKQS PGYQTELVIQ LVWVGGEPPQ QITSLAVNSS YGLVVFGNCN GIAMVDYLQK 

       670        680        690        700        710        720 
AVLLNLGTIE LYGSNDPYRR EPRSPRKSRQ PSGAGLCDIS EGTVVPEDRC KSPTSGSSSP 

       730        740        750        760        770        780 
HNSDDEQKMN NFIEKVKTKS RKFSKMVAND IAKMSRKLSL PTDLKPDLDV KDNSFSRSRS 

       790        800        810        820        830        840 
SSVTSIDKES REAISALHFC ETFTRKTDSS PSPCLWVGTT LGTVLVIALN LPPGGEQRLL 

       850        860        870        880        890        900 
QPVIVSPSGT ILRLKGAILR MAFLDTTGCL IPPAYEPWRE HNVPEEKDEK EKLKKRRPVS 

       910        920        930        940        950        960 
VSPSSSQEIS ENQYAVICSE KQAKVISLPT QNCAYKQNIT ETSFVLRGDI VALSNSICLA 

       970        980        990       1000       1010       1020 
CFCANGHIMT FSLPSLRPLL DVYYLPLTNM RIARTFCFTN NGQALYLVSP TEIQRLTYSQ 

      1030       1040       1050       1060       1070       1080 
ETCENLQEML GELFTPVETP EAPNRGFFKG LFGGGAQSLD REELFGESSS GKASRSLAQH 

      1090       1100       1110       1120       1130       1140 
IPGPGGIEGV KGAASGVVGE LARARLALDE RGQKLGDLEE RTAAMLSSAE SFSKHAHEIM 

      1150 
LKYKDKKWYQ F 

« Hide

Isoform 2 [UniParc].

Checksum: 2F1874662D9B30F6
Show »

FASTA1,115123,492
Isoform 3 [UniParc].

Checksum: B8EAE43977084610
Show »

FASTA1,098121,597

References

[1]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Cerebellum.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 373-1151 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 670-1151 (ISOFORM 2).
Tissue: Brain.
[4]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-762, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-759, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-785, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL356415, AL355365, AL590709 Genomic DNA. Translation: CAI20239.1.
AL356415, AL355365, AL590709 Genomic DNA. Translation: CAI20240.1.
AL356415, AL355365, AL590709 Genomic DNA. Translation: CAI20241.1.
AL355365, AL356415, AL590709 Genomic DNA. Translation: CAI40423.1.
AL355365, AL356415, AL590709 Genomic DNA. Translation: CAI40424.1.
AL355365, AL356415, AL590709 Genomic DNA. Translation: CAI40425.1.
AL590709, AL355365, AL356415 Genomic DNA. Translation: CAI40739.1.
AL590709, AL355365, AL356415 Genomic DNA. Translation: CAI40740.1.
AL590709, AL355365, AL356415 Genomic DNA. Translation: CAI40741.1.
BC113382 mRNA. Translation: AAI13383.1.
AK055484 mRNA. Translation: BAB70930.1.
AK090549 mRNA. Translation: BAC03475.1. Different initiation.
CCDSCCDS47499.1. [Q5T5C0-1]
CCDS5211.1. [Q5T5C0-2]
RefSeqNP_001121187.1. NM_001127715.2. [Q5T5C0-1]
NP_640337.3. NM_139244.4. [Q5T5C0-2]
UniGeneHs.736439.
Hs.86320.

3D structure databases

ProteinModelPortalQ5T5C0.
SMRQ5T5C0. Positions 1087-1143.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126417. 5 interactions.
IntActQ5T5C0. 1 interaction.
STRING9606.ENSP00000321826.

PTM databases

PhosphoSiteQ5T5C0.

Polymorphism databases

DMDM74762236.

Proteomic databases

MaxQBQ5T5C0.
PaxDbQ5T5C0.
PRIDEQ5T5C0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000321680; ENSP00000321826; ENSG00000164506. [Q5T5C0-1]
ENST00000367480; ENSP00000356450; ENSG00000164506. [Q5T5C0-3]
ENST00000367481; ENSP00000356451; ENSG00000164506. [Q5T5C0-2]
GeneID134957.
KEGGhsa:134957.
UCSCuc003qlz.3. human. [Q5T5C0-1]
uc010khz.2. human. [Q5T5C0-2]

Organism-specific databases

CTD134957.
GeneCardsGC06P147566.
H-InvDBHIX0019766.
HGNCHGNC:19665. STXBP5.
HPAHPA039991.
HPA049727.
MIM604586. gene.
neXtProtNX_Q5T5C0.
PharmGKBPA134954258.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG289584.
HOGENOMHOG000110929.
HOVERGENHBG083064.
InParanoidQ5T5C0.
KOK08518.
OMARYTHDEA.
OrthoDBEOG7DVD96.
PhylomeDBQ5T5C0.
TreeFamTF314585.

Enzyme and pathway databases

SignaLinkQ5T5C0.

Gene expression databases

ArrayExpressQ5T5C0.
BgeeQ5T5C0.
CleanExHS_STXBP5.
GenevestigatorQ5T5C0.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSPR00962. LETHAL2GIANT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 6 hits.
PROSITEPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSTXBP5. human.
GeneWikiSTXBP5.
GenomeRNAi134957.
NextBio83439.
PROQ5T5C0.
SOURCESearch...

Entry information

Entry nameSTXB5_HUMAN
AccessionPrimary (citable) accession number: Q5T5C0
Secondary accession number(s): Q14DF3 expand/collapse secondary AC list , Q5T5C1, Q5T5C2, Q8NBG8, Q96NG9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM