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Q5T4B2

- GT253_HUMAN

UniProt

Q5T4B2 - GT253_HUMAN

Protein

Probable inactive glycosyltransferase 25 family member 3

Gene

CERCAM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity.2 Publications

    GO - Biological processi

    1. cell adhesion Source: MGI
    2. cellular component movement Source: ProtInc
    3. leukocyte cell-cell adhesion Source: ProtInc
    4. lipopolysaccharide biosynthetic process Source: InterPro

    Keywords - Biological processi

    Cell adhesion

    Protein family/group databases

    CAZyiGT25. Glycosyltransferase Family 25.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable inactive glycosyltransferase 25 family member 3
    Alternative name(s):
    Cerebral endothelial cell adhesion molecule
    Gene namesi
    Name:CERCAM
    Synonyms:CEECAM1, GLT25D3, KIAA1502
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:23723. CERCAM.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162382177.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 595573Probable inactive glycosyltransferase 25 family member 3PRO_0000309544Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ5T4B2.
    PaxDbiQ5T4B2.
    PRIDEiQ5T4B2.

    PTM databases

    PhosphoSiteiQ5T4B2.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highly expressed in secretory and nervous tissues.2 Publications

    Gene expression databases

    ArrayExpressiQ5T4B2.
    BgeeiQ5T4B2.
    CleanExiHS_CERCAM.
    GenevestigatoriQ5T4B2.

    Organism-specific databases

    HPAiHPA021657.
    HPA051595.

    Interactioni

    Protein-protein interaction databases

    BioGridi119332. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5T4B2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi592 – 5954Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyltransferase 25 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3306.
    HOVERGENiHBG058097.
    InParanoidiQ5T4B2.
    OMAiEHRYGYM.
    OrthoDBiEOG7060RC.
    PhylomeDBiQ5T4B2.
    TreeFamiTF313826.

    Family and domain databases

    InterProiIPR002654. Glyco_trans_25.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF01755. Glyco_transf_25. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5T4B2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL    50
    GALERLDYPR ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG 100
    EPRFYPDEEG PKHWTKERHQ FLMELKQEAL TFARNWGADY ILFADTDNIL 150
    TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF WCGITPQGYY RRTAEYFPTK 200
    NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW PFDDIIVFAY 250
    ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR 300
    MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV 350
    VDAVDGWMLN SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE 400
    EVVARGLARV LVFEDDVRFE SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ 450
    VNPEKETAVE GLPGLVVAGY SYWTLAYALR LAGARKLLAS QPLRRMLPVD 500
    EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA GDAEWLSDTE 550
    TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL 595
    Length:595
    Mass (Da):67,592
    Last modified:December 21, 2004 - v1
    Checksum:iFC2157AD8C7AC558
    GO
    Isoform 2 (identifier: Q5T4B2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-78: Missing.

    Show »
    Length:517
    Mass (Da):59,100
    Checksum:i8E8C443064488AE7
    GO

    Sequence cautioni

    The sequence AAH98432.2 differs from that shown. Reason: Erroneous initiation.
    The sequence AAI19699.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAI19700.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA96026.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC11040.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAI13495.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti209 – 2091R → H in AAI19700. (PubMed:15489334)Curated
    Sequence conflicti356 – 3583GWM → AGW in AAD51367. (PubMed:10608765)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7878Missing in isoform 2. 3 PublicationsVSP_029234Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177203 mRNA. Translation: AAD51367.1.
    AK074519 mRNA. Translation: BAC11036.1.
    AK074523 mRNA. Translation: BAC11040.1. Different initiation.
    AL359091 Genomic DNA. Translation: CAI13494.1.
    AL359091 Genomic DNA. Translation: CAI13495.1. Sequence problems.
    AL359091 Genomic DNA. Translation: CAI13496.1.
    CH471090 Genomic DNA. Translation: EAW87787.1.
    CH471090 Genomic DNA. Translation: EAW87789.1.
    BC011811 mRNA. Translation: AAH11811.2.
    BC098432 mRNA. Translation: AAH98432.2. Different initiation.
    BC108698 mRNA. Translation: AAI08699.1.
    BC119698 mRNA. Translation: AAI19699.1. Different initiation.
    BC119699 mRNA. Translation: AAI19700.1. Different initiation.
    AB040935 mRNA. Translation: BAA96026.1. Different initiation.
    CCDSiCCDS6901.2. [Q5T4B2-1]
    CCDS69675.1. [Q5T4B2-2]
    RefSeqiNP_001273689.1. NM_001286760.1. [Q5T4B2-2]
    NP_057258.3. NM_016174.4. [Q5T4B2-1]
    XP_005252092.1. XM_005252035.1. [Q5T4B2-1]
    XP_005252094.1. XM_005252037.1. [Q5T4B2-2]
    XP_006717200.1. XM_006717137.1. [Q5T4B2-2]
    UniGeneiHs.495230.

    Genome annotation databases

    EnsembliENST00000372838; ENSP00000361929; ENSG00000167123. [Q5T4B2-1]
    ENST00000372842; ENSP00000361933; ENSG00000167123. [Q5T4B2-2]
    GeneIDi51148.
    KEGGihsa:51148.
    UCSCiuc004buy.1. human. [Q5T4B2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177203 mRNA. Translation: AAD51367.1 .
    AK074519 mRNA. Translation: BAC11036.1 .
    AK074523 mRNA. Translation: BAC11040.1 . Different initiation.
    AL359091 Genomic DNA. Translation: CAI13494.1 .
    AL359091 Genomic DNA. Translation: CAI13495.1 . Sequence problems.
    AL359091 Genomic DNA. Translation: CAI13496.1 .
    CH471090 Genomic DNA. Translation: EAW87787.1 .
    CH471090 Genomic DNA. Translation: EAW87789.1 .
    BC011811 mRNA. Translation: AAH11811.2 .
    BC098432 mRNA. Translation: AAH98432.2 . Different initiation.
    BC108698 mRNA. Translation: AAI08699.1 .
    BC119698 mRNA. Translation: AAI19699.1 . Different initiation.
    BC119699 mRNA. Translation: AAI19700.1 . Different initiation.
    AB040935 mRNA. Translation: BAA96026.1 . Different initiation.
    CCDSi CCDS6901.2. [Q5T4B2-1 ]
    CCDS69675.1. [Q5T4B2-2 ]
    RefSeqi NP_001273689.1. NM_001286760.1. [Q5T4B2-2 ]
    NP_057258.3. NM_016174.4. [Q5T4B2-1 ]
    XP_005252092.1. XM_005252035.1. [Q5T4B2-1 ]
    XP_005252094.1. XM_005252037.1. [Q5T4B2-2 ]
    XP_006717200.1. XM_006717137.1. [Q5T4B2-2 ]
    UniGenei Hs.495230.

    3D structure databases

    ProteinModelPortali Q5T4B2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119332. 3 interactions.

    Protein family/group databases

    CAZyi GT25. Glycosyltransferase Family 25.

    PTM databases

    PhosphoSitei Q5T4B2.

    Proteomic databases

    MaxQBi Q5T4B2.
    PaxDbi Q5T4B2.
    PRIDEi Q5T4B2.

    Protocols and materials databases

    DNASUi 51148.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000372838 ; ENSP00000361929 ; ENSG00000167123 . [Q5T4B2-1 ]
    ENST00000372842 ; ENSP00000361933 ; ENSG00000167123 . [Q5T4B2-2 ]
    GeneIDi 51148.
    KEGGi hsa:51148.
    UCSCi uc004buy.1. human. [Q5T4B2-1 ]

    Organism-specific databases

    CTDi 51148.
    GeneCardsi GC09P131174.
    H-InvDB HIX0008429.
    HGNCi HGNC:23723. CERCAM.
    HPAi HPA021657.
    HPA051595.
    neXtProti NX_Q5T4B2.
    PharmGKBi PA162382177.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG3306.
    HOVERGENi HBG058097.
    InParanoidi Q5T4B2.
    OMAi EHRYGYM.
    OrthoDBi EOG7060RC.
    PhylomeDBi Q5T4B2.
    TreeFami TF313826.

    Miscellaneous databases

    ChiTaRSi CERCAM. human.
    GenomeRNAii 51148.
    NextBioi 54025.
    PROi Q5T4B2.

    Gene expression databases

    ArrayExpressi Q5T4B2.
    Bgeei Q5T4B2.
    CleanExi HS_CERCAM.
    Genevestigatori Q5T4B2.

    Family and domain databases

    InterProi IPR002654. Glyco_trans_25.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF01755. Glyco_transf_25. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cerebral cell adhesion molecule: a novel leukocyte adhesion determinant on blood-brain barrier capillary endothelium."
      Starzyk R.M., Rosenow C., Frye J., Leismann M., Rodzinski E., Putney S., Tuomanen E.I.
      J. Infect. Dis. 181:181-187(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Brain capillary.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-595 (ISOFORM 1).
      Tissue: Embryo.
    3. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-595 (ISOFORMS 1/2).
      Tissue: Muscle, Ovary and PNS.
    6. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-561 (ISOFORM 2).
      Tissue: Brain.
    7. "Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
      Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
      Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, LACK OF GALACTOSYLTRANSFERASE ACTIVITY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGT253_HUMAN
    AccessioniPrimary (citable) accession number: Q5T4B2
    Secondary accession number(s): A7MD00
    , C4AMA2, Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5, Q9P226, Q9UMW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3