Q5T4B2 (GT253_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inactive glycosyltransferase 25 family member 3

Alternative name(s):
Cerebral endothelial cell adhesion molecule

Gene names

Name:	CERCAM
Synonyms:	CEECAM1, GLT25D3, KIAA1502

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity. Ref.1 Ref.7
Subcellular location	Endoplasmic reticulum lumen Potential.
Tissue specificity	Ubiquitous. Highly expressed in secretory and nervous tissues. Ref.1 Ref.7
Sequence similarities	Belongs to the glycosyltransferase 25 family.
Caution	Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.
Sequence caution	The sequence AAH98432.2 differs from that shown. Reason: Erroneous initiation. The sequence AAI19699.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI19700.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA96026.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11040.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI13495.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Traceable author statement Ref.1. Source: ProtInc leukocyte cell-cell adhesion Traceable author statement Ref.1. Source: ProtInc lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Non-traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5T4B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5T4B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 595	573	Probable inactive glycosyltransferase 25 family member 3		PRO_0000309544
Regions
	Motif	592 – 595	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	237	1	N-linked (GlcNAc...) Potential
	Glycosylation	360	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 78	78	Missing in isoform 2.		VSP_029234
Experimental info
	Sequence conflict	209	1	R → H in AAI19700. Ref.5
	Sequence conflict	356 – 358	3	GWM → AGW in AAD51367. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: FC2157AD8C7AC558	FASTA	595	67,592
	10 20 30 40 50 60 MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL GALERLDYPR 70 80 90 100 110 120 ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG EPRFYPDEEG PKHWTKERHQ 130 140 150 160 170 180 FLMELKQEAL TFARNWGADY ILFADTDNIL TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF 190 200 210 220 230 240 WCGITPQGYY RRTAEYFPTK NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW 250 260 270 280 290 300 PFDDIIVFAY ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR 310 320 330 340 350 360 MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV VDAVDGWMLN 370 380 390 400 410 420 SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE EVVARGLARV LVFEDDVRFE 430 440 450 460 470 480 SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ VNPEKETAVE GLPGLVVAGY SYWTLAYALR 490 500 510 520 530 540 LAGARKLLAS QPLRRMLPVD EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA 550 560 570 580 590 GDAEWLSDTE TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL
	Isoform 2 [UniParc]. Checksum: 8E8C443064488AE7	FASTA	517	59,100
	10 20 30 40 50 60 MLQEWLAAVG DDYAAVVWRP EGEPRFYPDE EGPKHWTKER HQFLMELKQE ALTFARNWGA 70 80 90 100 110 120 DYILFADTDN ILTNNQTLRL LMGQGLPVVA PMLDSQTYYS NFWCGITPQG YYRRTAEYFP 130 140 150 160 170 180 TKNRQRRGCF RVPMVHSTFL ASLRAEGADQ LAFYPPHPNY TWPFDDIIVF AYACQAAGVS 190 200 210 220 230 240 VHVCNEHRYG YMNVPVKSHQ GLEDERVNFI HLILEALVDG PRMQASAHVT RPSKRPSKIG 250 260 270 280 290 300 FDEVFVISLA RRPDRRERML ASLWEMEISG RVVDAVDGWM LNSSAIRNLG VDLLPGYQDP 310 320 330 340 350 360 YSGRTLTKGE VGCFLSHYSI WEEVVARGLA RVLVFEDDVR FESNFRGRLE RLMEDVEAEK 370 380 390 400 410 420 LSWDLIYLGR KQVNPEKETA VEGLPGLVVA GYSYWTLAYA LRLAGARKLL ASQPLRRMLP 430 440 450 460 470 480 VDEFLPIMFD QHPNEQYKAH FWPRDLVAFS AQPLLAAPTH YAGDAEWLSD TETSSPWDDD 490 500 510 SGRLISWSGS QKTLRSPRLD LTGSSGHSLQ PQPRDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cerebral cell adhesion molecule: a novel leukocyte adhesion determinant on blood-brain barrier capillary endothelium." Starzyk R.M., Rosenow C., Frye J., Leismann M., Rodzinski E., Putney S., Tuomanen E.I. J. Infect. Dis. 181:181-187(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Brain capillary.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-595 (ISOFORM 1). Tissue: Embryo.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-595 (ISOFORMS 1/2). Tissue: Muscle, Ovary and PNS.
[6]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-561 (ISOFORM 2). Tissue: Brain.
[7]	"Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases." Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T. Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, LACK OF GALACTOSYLTRANSFERASE ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177203 mRNA. Translation: AAD51367.1. AK074519 mRNA. Translation: BAC11036.1. AK074523 mRNA. Translation: BAC11040.1. Different initiation. AL359091 Genomic DNA. Translation: CAI13494.1. AL359091 Genomic DNA. Translation: CAI13495.1. Sequence problems. AL359091 Genomic DNA. Translation: CAI13496.1. CH471090 Genomic DNA. Translation: EAW87787.1. CH471090 Genomic DNA. Translation: EAW87789.1. BC011811 mRNA. Translation: AAH11811.2. BC098432 mRNA. Translation: AAH98432.2. Different initiation. BC108698 mRNA. Translation: AAI08699.1. BC119698 mRNA. Translation: AAI19699.1. Different initiation. BC119699 mRNA. Translation: AAI19700.1. Different initiation. AB040935 mRNA. Translation: BAA96026.1. Different initiation.
IPI	IPI00295595. IPI00941005.
RefSeq	NP_057258.3. NM_016174.4.
UniGene	Hs.495230.
3D structure databases
ProteinModelPortal	Q5T4B2.
ModBase	Search...
Protein family/group databases
CAZy	GT25. Glycosyltransferase Family 25.
PTM databases
PhosphoSite	Q5T4B2.
Polymorphism databases
DMDM	74744901.
Proteomic databases
PaxDb	Q5T4B2.
PRIDE	Q5T4B2.
Protocols and materials databases
DNASU	51148.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372838; ENSP00000361929; ENSG00000167123. ENST00000372842; ENSP00000361933; ENSG00000167123. ENST00000420512; ENSP00000416676; ENSG00000167123.
GeneID	51148.
KEGG	hsa:51148.
UCSC	uc004buy.1. human.
Organism-specific databases
CTD	51148.
GeneCards	GC09P131174.
H-InvDB	HIX0008429.
HGNC	HGNC:23723. CERCAM.
HPA	HPA021657.
neXtProt	NX_Q5T4B2.
PharmGKB	PA162382177.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG3306.
HOVERGEN	HBG058097.
InParanoid	Q5T4B2.
OMA	EYFPTKN.
OrthoDB	EOG4640BH.
Gene expression databases
ArrayExpress	Q5T4B2.
Bgee	Q5T4B2.
CleanEx	HS_CERCAM.
Genevestigator	Q5T4B2.
Family and domain databases
InterPro	IPR002654. Glyco_trans_25. [Graphical view]
Pfam	PF01755. Glyco_transf_25. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CERCAM. human.
GenomeRNAi	51148.
NextBio	54025.

Entry information

Entry name

GT253_HUMAN

Accession

Primary (citable) accession number: Q5T4B2
Secondary accession number(s): A7MD00 , C4AMA2, Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5, Q9P226, Q9UMW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.