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Q5T4B2 (GT253_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable inactive glycosyltransferase 25 family member 3
Alternative name(s):
Cerebral endothelial cell adhesion molecule
Gene names
Name:CERCAM
Synonyms:CEECAM1, GLT25D3, KIAA1502
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity. Ref.1 Ref.7

Subcellular location

Endoplasmic reticulum lumen Potential.

Tissue specificity

Ubiquitous. Highly expressed in secretory and nervous tissues. Ref.1 Ref.7

Sequence similarities

Belongs to the glycosyltransferase 25 family.

Caution

Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.

Sequence caution

The sequence AAH98432.2 differs from that shown. Reason: Erroneous initiation.

The sequence AAI19699.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAI19700.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA96026.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC11040.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI13495.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5T4B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5T4B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 595573Probable inactive glycosyltransferase 25 family member 3
PRO_0000309544

Regions

Motif592 – 5954Prevents secretion from ER Potential

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation2371N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 7878Missing in isoform 2.
VSP_029234

Experimental info

Sequence conflict2091R → H in AAI19700. Ref.5
Sequence conflict356 – 3583GWM → AGW in AAD51367. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: FC2157AD8C7AC558

FASTA59567,592
        10         20         30         40         50         60 
MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL GALERLDYPR 

        70         80         90        100        110        120 
ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG EPRFYPDEEG PKHWTKERHQ 

       130        140        150        160        170        180 
FLMELKQEAL TFARNWGADY ILFADTDNIL TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF 

       190        200        210        220        230        240 
WCGITPQGYY RRTAEYFPTK NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW 

       250        260        270        280        290        300 
PFDDIIVFAY ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR 

       310        320        330        340        350        360 
MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV VDAVDGWMLN 

       370        380        390        400        410        420 
SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE EVVARGLARV LVFEDDVRFE 

       430        440        450        460        470        480 
SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ VNPEKETAVE GLPGLVVAGY SYWTLAYALR 

       490        500        510        520        530        540 
LAGARKLLAS QPLRRMLPVD EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA 

       550        560        570        580        590 
GDAEWLSDTE TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL 

« Hide

Isoform 2 [UniParc].

Checksum: 8E8C443064488AE7
Show »

FASTA51759,100

References

« Hide 'large scale' references
[1]"Cerebral cell adhesion molecule: a novel leukocyte adhesion determinant on blood-brain barrier capillary endothelium."
Starzyk R.M., Rosenow C., Frye J., Leismann M., Rodzinski E., Putney S., Tuomanen E.I.
J. Infect. Dis. 181:181-187(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Brain capillary.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-595 (ISOFORM 1).
Tissue: Embryo.
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-595 (ISOFORMS 1/2).
Tissue: Muscle, Ovary and PNS.
[6]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-561 (ISOFORM 2).
Tissue: Brain.
[7]"Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, LACK OF GALACTOSYLTRANSFERASE ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177203 mRNA. Translation: AAD51367.1.
AK074519 mRNA. Translation: BAC11036.1.
AK074523 mRNA. Translation: BAC11040.1. Different initiation.
AL359091 Genomic DNA. Translation: CAI13494.1.
AL359091 Genomic DNA. Translation: CAI13495.1. Sequence problems.
AL359091 Genomic DNA. Translation: CAI13496.1.
CH471090 Genomic DNA. Translation: EAW87787.1.
CH471090 Genomic DNA. Translation: EAW87789.1.
BC011811 mRNA. Translation: AAH11811.2.
BC098432 mRNA. Translation: AAH98432.2. Different initiation.
BC108698 mRNA. Translation: AAI08699.1.
BC119698 mRNA. Translation: AAI19699.1. Different initiation.
BC119699 mRNA. Translation: AAI19700.1. Different initiation.
AB040935 mRNA. Translation: BAA96026.1. Different initiation.
CCDSCCDS6901.2. [Q5T4B2-1]
CCDS69675.1. [Q5T4B2-2]
RefSeqNP_001273689.1. NM_001286760.1. [Q5T4B2-2]
NP_057258.3. NM_016174.4. [Q5T4B2-1]
XP_005252092.1. XM_005252035.1. [Q5T4B2-1]
XP_005252094.1. XM_005252037.1. [Q5T4B2-2]
XP_006717200.1. XM_006717137.1. [Q5T4B2-2]
UniGeneHs.495230.

3D structure databases

ProteinModelPortalQ5T4B2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119332. 3 interactions.

Protein family/group databases

CAZyGT25. Glycosyltransferase Family 25.

PTM databases

PhosphoSiteQ5T4B2.

Proteomic databases

MaxQBQ5T4B2.
PaxDbQ5T4B2.
PRIDEQ5T4B2.

Protocols and materials databases

DNASU51148.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000372838; ENSP00000361929; ENSG00000167123. [Q5T4B2-1]
ENST00000372842; ENSP00000361933; ENSG00000167123. [Q5T4B2-2]
GeneID51148.
KEGGhsa:51148.
UCSCuc004buy.1. human. [Q5T4B2-1]

Organism-specific databases

CTD51148.
GeneCardsGC09P131174.
H-InvDBHIX0008429.
HGNCHGNC:23723. CERCAM.
HPAHPA021657.
HPA051595.
neXtProtNX_Q5T4B2.
PharmGKBPA162382177.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG3306.
HOVERGENHBG058097.
InParanoidQ5T4B2.
OMAEHRYGYM.
OrthoDBEOG7060RC.
PhylomeDBQ5T4B2.
TreeFamTF313826.

Gene expression databases

ArrayExpressQ5T4B2.
BgeeQ5T4B2.
CleanExHS_CERCAM.
GenevestigatorQ5T4B2.

Family and domain databases

InterProIPR002654. Glyco_trans_25.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF01755. Glyco_transf_25. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCERCAM. human.
GenomeRNAi51148.
NextBio54025.
PROQ5T4B2.

Entry information

Entry nameGT253_HUMAN
AccessionPrimary (citable) accession number: Q5T4B2
Secondary accession number(s): A7MD00 expand/collapse secondary AC list , C4AMA2, Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5, Q9P226, Q9UMW5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM