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Protein

Probable inactive glycosyltransferase 25 family member 3

Gene

CERCAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Has apparently no beta-galactosyltransferase activity.2 Publications

GO - Biological processi

  1. cell adhesion Source: MGI
  2. leukocyte cell-cell adhesion Source: ProtInc
  3. lipopolysaccharide biosynthetic process Source: InterPro
  4. movement of cell or subcellular component Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Protein family/group databases

CAZyiGT25. Glycosyltransferase Family 25.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable inactive glycosyltransferase 25 family member 3
Alternative name(s):
Cerebral endothelial cell adhesion molecule
Gene namesi
Name:CERCAM
Synonyms:CEECAM1, GLT25D3, KIAA1502
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:23723. CERCAM.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382177.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 595573Probable inactive glycosyltransferase 25 family member 3PRO_0000309544Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ5T4B2.
PaxDbiQ5T4B2.
PRIDEiQ5T4B2.

PTM databases

PhosphoSiteiQ5T4B2.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in secretory and nervous tissues.2 Publications

Gene expression databases

BgeeiQ5T4B2.
CleanExiHS_CERCAM.
ExpressionAtlasiQ5T4B2. baseline and differential.
GenevestigatoriQ5T4B2.

Organism-specific databases

HPAiHPA021657.
HPA051595.

Interactioni

Protein-protein interaction databases

BioGridi119332. 14 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ5T4B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi592 – 5954Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase 25 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3306.
GeneTreeiENSGT00550000074427.
HOVERGENiHBG058097.
InParanoidiQ5T4B2.
OMAiEHRYGYM.
OrthoDBiEOG7060RC.
PhylomeDBiQ5T4B2.
TreeFamiTF313826.

Family and domain databases

InterProiIPR002654. Glyco_trans_25.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01755. Glyco_transf_25. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T4B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL
60 70 80 90 100
GALERLDYPR ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG
110 120 130 140 150
EPRFYPDEEG PKHWTKERHQ FLMELKQEAL TFARNWGADY ILFADTDNIL
160 170 180 190 200
TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF WCGITPQGYY RRTAEYFPTK
210 220 230 240 250
NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW PFDDIIVFAY
260 270 280 290 300
ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR
310 320 330 340 350
MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV
360 370 380 390 400
VDAVDGWMLN SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE
410 420 430 440 450
EVVARGLARV LVFEDDVRFE SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ
460 470 480 490 500
VNPEKETAVE GLPGLVVAGY SYWTLAYALR LAGARKLLAS QPLRRMLPVD
510 520 530 540 550
EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA GDAEWLSDTE
560 570 580 590
TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL
Length:595
Mass (Da):67,592
Last modified:December 21, 2004 - v1
Checksum:iFC2157AD8C7AC558
GO
Isoform 2 (identifier: Q5T4B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:517
Mass (Da):59,100
Checksum:i8E8C443064488AE7
GO

Sequence cautioni

The sequence AAH98432.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAI19699.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAI19700.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA96026.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC11040.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI13495.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091R → H in AAI19700 (PubMed:15489334).Curated
Sequence conflicti356 – 3583GWM → AGW in AAD51367 (PubMed:10608765).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7878Missing in isoform 2. 3 PublicationsVSP_029234Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177203 mRNA. Translation: AAD51367.1.
AK074519 mRNA. Translation: BAC11036.1.
AK074523 mRNA. Translation: BAC11040.1. Different initiation.
AL359091 Genomic DNA. Translation: CAI13494.1.
AL359091 Genomic DNA. Translation: CAI13495.1. Sequence problems.
AL359091 Genomic DNA. Translation: CAI13496.1.
CH471090 Genomic DNA. Translation: EAW87787.1.
CH471090 Genomic DNA. Translation: EAW87789.1.
BC011811 mRNA. Translation: AAH11811.2.
BC098432 mRNA. Translation: AAH98432.2. Different initiation.
BC108698 mRNA. Translation: AAI08699.1.
BC119698 mRNA. Translation: AAI19699.1. Different initiation.
BC119699 mRNA. Translation: AAI19700.1. Different initiation.
AB040935 mRNA. Translation: BAA96026.1. Different initiation.
CCDSiCCDS6901.2. [Q5T4B2-1]
CCDS69675.1. [Q5T4B2-2]
RefSeqiNP_001273689.1. NM_001286760.1. [Q5T4B2-2]
NP_057258.3. NM_016174.4. [Q5T4B2-1]
XP_005252092.1. XM_005252035.1. [Q5T4B2-1]
XP_005252094.1. XM_005252037.1. [Q5T4B2-2]
XP_006717200.1. XM_006717137.1. [Q5T4B2-2]
UniGeneiHs.495230.

Genome annotation databases

EnsembliENST00000372838; ENSP00000361929; ENSG00000167123. [Q5T4B2-1]
ENST00000372842; ENSP00000361933; ENSG00000167123. [Q5T4B2-2]
GeneIDi51148.
KEGGihsa:51148.
UCSCiuc004buy.1. human. [Q5T4B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177203 mRNA. Translation: AAD51367.1.
AK074519 mRNA. Translation: BAC11036.1.
AK074523 mRNA. Translation: BAC11040.1. Different initiation.
AL359091 Genomic DNA. Translation: CAI13494.1.
AL359091 Genomic DNA. Translation: CAI13495.1. Sequence problems.
AL359091 Genomic DNA. Translation: CAI13496.1.
CH471090 Genomic DNA. Translation: EAW87787.1.
CH471090 Genomic DNA. Translation: EAW87789.1.
BC011811 mRNA. Translation: AAH11811.2.
BC098432 mRNA. Translation: AAH98432.2. Different initiation.
BC108698 mRNA. Translation: AAI08699.1.
BC119698 mRNA. Translation: AAI19699.1. Different initiation.
BC119699 mRNA. Translation: AAI19700.1. Different initiation.
AB040935 mRNA. Translation: BAA96026.1. Different initiation.
CCDSiCCDS6901.2. [Q5T4B2-1]
CCDS69675.1. [Q5T4B2-2]
RefSeqiNP_001273689.1. NM_001286760.1. [Q5T4B2-2]
NP_057258.3. NM_016174.4. [Q5T4B2-1]
XP_005252092.1. XM_005252035.1. [Q5T4B2-1]
XP_005252094.1. XM_005252037.1. [Q5T4B2-2]
XP_006717200.1. XM_006717137.1. [Q5T4B2-2]
UniGeneiHs.495230.

3D structure databases

ProteinModelPortaliQ5T4B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119332. 14 interactions.

Protein family/group databases

CAZyiGT25. Glycosyltransferase Family 25.

PTM databases

PhosphoSiteiQ5T4B2.

Proteomic databases

MaxQBiQ5T4B2.
PaxDbiQ5T4B2.
PRIDEiQ5T4B2.

Protocols and materials databases

DNASUi51148.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372838; ENSP00000361929; ENSG00000167123. [Q5T4B2-1]
ENST00000372842; ENSP00000361933; ENSG00000167123. [Q5T4B2-2]
GeneIDi51148.
KEGGihsa:51148.
UCSCiuc004buy.1. human. [Q5T4B2-1]

Organism-specific databases

CTDi51148.
GeneCardsiGC09P131174.
H-InvDBHIX0008429.
HGNCiHGNC:23723. CERCAM.
HPAiHPA021657.
HPA051595.
neXtProtiNX_Q5T4B2.
PharmGKBiPA162382177.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG3306.
GeneTreeiENSGT00550000074427.
HOVERGENiHBG058097.
InParanoidiQ5T4B2.
OMAiEHRYGYM.
OrthoDBiEOG7060RC.
PhylomeDBiQ5T4B2.
TreeFamiTF313826.

Miscellaneous databases

ChiTaRSiCERCAM. human.
GenomeRNAii51148.
NextBioi54025.
PROiQ5T4B2.

Gene expression databases

BgeeiQ5T4B2.
CleanExiHS_CERCAM.
ExpressionAtlasiQ5T4B2. baseline and differential.
GenevestigatoriQ5T4B2.

Family and domain databases

InterProiIPR002654. Glyco_trans_25.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01755. Glyco_transf_25. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cerebral cell adhesion molecule: a novel leukocyte adhesion determinant on blood-brain barrier capillary endothelium."
    Starzyk R.M., Rosenow C., Frye J., Leismann M., Rodzinski E., Putney S., Tuomanen E.I.
    J. Infect. Dis. 181:181-187(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain capillary.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-595 (ISOFORM 1).
    Tissue: Embryo.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-595 (ISOFORMS 1/2).
    Tissue: Muscle, Ovary and PNS.
  6. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-561 (ISOFORM 2).
    Tissue: Brain.
  7. "Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
    Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
    Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, LACK OF GALACTOSYLTRANSFERASE ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGT253_HUMAN
AccessioniPrimary (citable) accession number: Q5T4B2
Secondary accession number(s): A7MD00
, C4AMA2, Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5, Q9P226, Q9UMW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: March 4, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.